检索结果-南通市图书馆

Degradation of intestinal mRNA:A matter of treatment

World Journal of Gastroenterology 2015年第12期21卷 3499-3508页

作者： Sabine Heumüller-Klug Carsten Sticht karin kaiser Elvira Wink Cornelia Hagl Lucas Wessel Karl-Herbert Schafer Department of Pediatric Surgery Mannheim Medical University of Heidelberg Medical Research Centre Mannheim Medical University of Heidelberg University of Applied Sciences kaiserslautern

AIM: To characterize the influence of location, species and treatment upon RNA degradation in tissue samples from the gastrointestinal tract. METHODS: The intestinal samples were stored in different medium for different times under varyingconditions: different species(human and rat), varying temperature(storage on crushed ice or room temperature), time point of dissection of the submucous-mucous layer from the smooth muscle(before or after storage), different rinsing methods(rinsing with Medium, PBS, RNALater or without rinsing at all) and different regions of the gut(proximal and distal small intestine, caecum, colon and rectum). The total RNA from different parts of the gut(rat: proximal and distal small intestine, caecum, colon and rectum, human: colon and rectum) and individual gut layers(muscle and submucosal/mucosal) was extracted. The quality of the RNA was assessed by micro capillary electrophoresis. The RNA quality was expressed by the RNA integrity number which is calculated from the relative height and area of the 18 S and 28 S RNA peaks. From rat distal small intestine q PCR was performed for neuronal and glial ***: RNA obtained from smooth muscle tissue is much longer stable than those from submucosal/mucosal tissue. At RT muscle RNA degrades after one day, on ice it is stable at least three days. Cleaning and separation of gut layers before storage and use of RNALater, maintains the stability of muscle RNA at RT for much longer periods. Different parts of the gut show varying degradation periods. RNA obtained from the submucosal/mucosal layer always showed a much worse amplification rate than RNA from muscle tissue. In general RNA harvested from rat tissue, either smooth muscle layer or submucosal/mucosal layer is much longer stable than RNA from human gut tissue, and RNA obtained from smooth muscle tissue shows an increased stability compared to RNA from submucosal/mucosal tissue. At RT muscle RNA degrades after one day, while the stabilit

关键词： Key words:Intestinal RNA Degradation Storing conditions Flushing Cooling

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Comparisons of oxbow lake fish assemblages in relation to bigheaded carp establishment in the lower White River, Arkansas

引用

Ecological Processes 2024年第1期13卷 188-205页

作者： Michael A.Eggleton Cody J.Salzmann Joseph E.kaiser Shannon C.F.Smith Department of Aquaculture and Fisheries University of Arkansas at Pine Bluff Arkansas Game and Fish Commission Virginia Institute of Marine Science

Background Since the late 1990s, bigheaded carps(largely silver carp [Hypophthalmichthys molitrix] but also bighead carp [H. nobilis]) have established throughout the lower Mississippi River basin. Using previously studied oxbow lakes in the lower White River basin, Arkansas, we compared current(2017, “post-carp” establishment) fish assemblages to historical(2002, “pre-carp” establishment) fish assemblages. Fish assemblages were comprehensively assessed using multiple gears, including boat electrofishing, mini-fyke nets, and experimental small-mesh gill *** T-tests suggested that fish assemblage indices of richness, diversity, evenness, and dominance were often greater(P 0.05) between the pre-carp and post-carp period with fish assemblages depicted using mini-fyke nets. Nonmetric multidimensional scaling analyses indicated that fish assemblages differed structurally between pre-carp and post-carp periods. Assemblage differences were linked to both small and large abundance changes for more than 20 species. Abundances of gizzard shad(Dorosoma cepedianum), emerald shiner(Notropis atherinoides), pugnose minnow(Opsopoeodus emiliae), crappies(Pomoxis spp.), bluegill(Lepomis macrochirus), orangespotted sunfish(L. humilis), and yellow bass(Morone mississippiensis) declined between the pre-carp and postcarp periods. Conversely, abundances of weed shiner(N. texanus), pallid shiner(Hybopsis amnis), longear sunfish(L. megalotis), buffalofishes(Ictiobus spp.), and gars(Lepisosteus spp.) generally increased during the same *** Although not possible to conclude assemblage shifts were entirely related to bigheaded carps due to the absence of an appropriate reference system where carps did not establish, the wide establishment of these carps is one of the most pervasive changes to have occurred in the lower White

关键词： Bigheaded carp Silver carp Aquatic nuisance species Fish assemblage Oxbow lake Large river

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Dual-Functional Electrode Promoting Dendrite-Free and CO_(2) Utilization Enabled High-Reversible Symmetric Na-CO_(2) Batteries

引用

Energy & Environmental Materials 2024年第3期7卷 123-132页

作者： Changfan Xu Jiajia Qiu Yulian Dong Yueliang Li Yonglong Shen Huaping Zhao Ute kaiser Guosheng Shao Yong Lei Fachgebiet Angewandte Nanophysik Institut für Physik&IMN MacroNanoTechnische Universität Ilmenau 98693Ilmenau Germany Central Facility for Electron Microscopy Electron Microscopy Group of Materials ScienceUlm UniversityUlm 89081Germany School of Materials Science and Engineering Zhengzhou UniversityZhengzhou 450001China

Sodium-carbon dioxide(Na-CO_(2))batteries are regarded as promising energy storage technologies because of their impressive theoretical energy density and CO_(2)reutilization,but their practical applications are restricted by uncontrollable sodium dendrite growth and poor electrochemical kinetics of CO_(2)*** suitable multifunctional electrodes for dendritefree anodes and kinetics-enhanced CO_(2)cathodes is considered one of the most important ways to advance the practical application of Na-CO_(2)***,RuO2 nanoparticles encapsulated in carbon paper(RuCP)are rationally designed and employed as both Na anode host and CO_(2)cathode in Na-CO_(2)*** outstanding sodiophilicity and high catalytic activity of RuCP electrodes can simultaneously contribute to homogenous Na+distribution and dendrite-free sodium structure at the anode,as well as strengthen discharge and charge kinetics at the *** morphological evolution confirmed the uniform deposition of Na on RuCP anode with dense and flat interfaces,delivering enhanced Coulombic efficiency of 99.5%and cycling stability near 1500 ***,Na-CO_(2)batteries with RuCP cathode demonstrated excellent cycling stability(>350 cycles).Significantly,implementation of a dendrite-free RuCP@Na anode and catalytic-site-rich RuCP cathode allowed for the construction of a symmetric Na-CO_(2)battery with long-duration cyclability,offering inspiration for extensive practical uses of Na-CO_(2)batteries.

关键词： CO_(2)cathode dendrite free electrocatalysis Na metal anode symmetric CO_(2)batteries

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Turning Trash into Treasure: Developing an Intelligent Bin for Plastic Bottle Recycling

引用

Journal of Social Computing 2024年第1期5卷 1-14页

作者： Sirajam Munira Niloy Paul Md Ashraful Alam Md.Mahmudur Rahman M.Shamim kaiser Institute of Information Technology Jahangirnagar UniversityDhaka 1342Bangladesh

Plastic pollution has emerged as a major global concern due to its enduring nature and limited recycling *** response to this critical challenge,this paper presents a novel approach utilizing a Detection-Based Reward System(DBRS)alongside an innovative business model to promote effective plastic waste management,reduce plastic waste accumulation in the nature,and uphold environmental *** the YOLOv5 algorithm for its exceptional accuracy,speed,and open-source availability,plastic bottle detection becomes a pivotal aspect of this *** seamlessly enroll in the system,triggering an automated detection process that computes reward points corresponding to their deposited plastic *** reward points are meticulously stored within a centralized *** its operational facets,this comprehensive system encompasses a robust business model,strategically poised to capture widespread engagement with waste disposal practices,thereby contributing to the realization of Sustainable Development Goals(SDGs)geared towards fostering a healthier ***,the DBRS attains cutting-edge performance in plastic bottle detection,boasting an impressive mean Average Precision(mAP)of 0.973,underscoring its efficacy in tackling plastic pollution.

关键词： smart environment reward system YOLOv5 deep learning business model

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Research and development of VUV optical coatings for micro mirror applications

引用

光学精密工程 2005年第4期13卷 465-470页

作者： Alexandre Gatto Norbert kaiser Fraunhofer IOF Albert-Einstein-Str. 7 D-07745 Jena GermanyFraunhofer IOF Albert-Einstein-Str. 7 D-07745 Jena Germany

This paper deals with vacuum UV optical coatings for micro mirror applications. High reflecting low-stress optical coatings have been developed for the next-generation of micro mechanical mirrors. The optimized metal systems are applicable in the VUV spectral region and can be integrated in the technology of MOEMS, such as spatial light modulators (SLM) and micro scanning mirrors.

关键词： VUV 光学涂覆技术微镜技术薄膜调节器

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A review of refinery complexity applications

引用

Petroleum Science 2017年第1期14卷 167-194页

作者： Mark J. kaiser Center for Energy Studies Louisiana State UniversityEnergy Coast and Environment Building Baton RougeLA 70803 USA

Refinery complexity quantifies the sophistication and capital intensity of a refinery and has found widespread application in facility classification, cost estimation, sales price models, and other uses. Despite the ubiquity and widespread use of refining complexity, however, surprisingly little material has been written on its applications. The pur- pose of this review is to describe the primary applications of refinery complexity and some recent extensions. A secondary purpose of this review is to provide a framework that unifies complexity applications and suggests avenues for future research. Examples illustrate the applications considered.

关键词： Cost estimation Functional complexityInverse problem Replacement cost Refineryclassification Sales price models

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Evolution and future of laparoscopic colorectal surgery

引用

World Journal of Gastroenterology 2014年第41期20卷 15119-15124页

作者： Andreas M kaiser Department of Colorectal Surgery Keck School of MedicineUniversity of Southern California

The advances of laparoscopic surgery since the early 1990 s have caused one of the largest technical revolutions in medicine since the detection of antibiotics(1922,Flemming),the discovery of DNA structure(1953,Watson and Crick),and solid organ transplantation(1954,Murray).Perseverance through a rocky start and increased familiarity with the chop-stick surgery in conjunction with technical refinements has resulted in a rapid expansion of the indications for minimally invasive ***-related factors initially contributed to this success and included the improved postoperative recovery and cosmesis,fewer wound complications,lower risk for incisional hernias and for subsequent adhesionrelated small bowel obstructions; the major breakthrough however came with favorable long-term outcomes data on oncological *** future will have to determine the specific role of various technical approaches,define prognostic factors of success and true progress,and consider directing further innovation while potentially limiting approaches that do not add to patient outcomes.

关键词： Laparoscopic Laparoscopy Open surgery Colorectal surgery Colectomy Robot

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

An operational approach to ground control in deep mines

引用

Journal of Rock Mechanics and Geotechnical Engineering 2022年第1期14卷 67-81页

作者： A.Moss P.K.kaiser Sonal Mining Technology Inc. 2488 Shadbolt LaneWest VancouverBCV7S 3J1Canada Laurentian University SudburyONP3E 2C6Canada GeoK Inc. 680 Ramsey Lake RoadSudburyONP3E 6H5Canada

As mines go deeper and get larger,mine designs become more fragile largely due to the response of the rock mass to *** control and rock support become important levers in the mine construction schedule,production performance,and excavation *** example,in cave mines,the production footprint together with associated mine infrastructure are significant investments in a modern caving *** investment must be protected and maintained to reduce the risk of ground-related production *** is necessary to preserve the health of these excavations and their maintenance through an effective rock support *** support thus becomes a strategic element in asset *** article focuses on support design for brittle ground when displacements induced by stress-fracturing consume much of the support’s *** deals with the functionality of the support in deforming *** interlinked concepts are important when assessing excavation *** must not only account for load equilibrium but also for deformation compatibility and capacity *** importantly,the support’s displacement capacity is being consumed when the rock mass is deformed after support ***,it is necessary to design for the support capacity remaining at the time when the support is *** support capacity can be consumed,it can also be restored by means of preventive support maintenance(PSM).This concept for cost-effective ground control is introduced and illustrated on operational ***,how design can impact construction costs and schedule are *** is installed to provide a safe environment and preserve an operationally functional *** also must assure senior management that investments in high quality support and its maintenance will substantially reduce delays and with it,*** is demonstrated that the use of‘gabion-like’support systems can achieve these goals.A technical summary of th

关键词： Ground control Rock support Deformation-based support design(DBSD) Gabion panel Deep mining Support consumption Preventive support maintenance(PSM) Asset management

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Influence of degree of interlock on confined strength of jointed hard rock masses

引用

Journal of Rock Mechanics and Geotechnical Engineering 2020年第6期12卷 1152-1170页

作者： Navid Bahrani Peter K.kaiser a Department of Civil and Resource Engineering Dalhousie UniversityHalifaxNova ScotiaCanada b Bharti School of Engineering Laurentian UniversitySudburyOntarioCanada

The strength of jointed rock mass is strongly controlled by the degree of interlock between its constituent rock *** degree of interlock constrains the kinematic freedom of individual rock blocks to rotate and slide along the block forming *** HoekeBrown(HB)failure criterion and the geological strength index(GSI)were developed based on experiences from mine slopes and tunneling projects in moderately to poorly interlocked jointed rock *** has since then been demonstrated that the approach to estimate the HB strength parameters based on the GSI strength scaling equations(called the‘GSI strength equations’)tends to underestimate the confined peak strength of highly interlocked jointed rock masses(***>65),where the rock mass is often non-persistently jointed,and the intact rock blocks are strong and *** estimation of the confined strength of such rock masses is relevant when designing mine pillars and abutments at great depths,where the confining pressure is high enough to prevent block rotation and free sliding on block *** this article,a grain-based distinct element modeling approach is used to simulate jointed rock masses of various degrees of interlock and to investigate the influences of block shape,joint persistence and joint surface condition on the confined peak *** focus is on non-persistently jointed and blocky(persistently jointed)rock masses,consisting of hard and homogeneous rock blocks devoid of any strength degrading defects such as *** results from this investigation confirm that the GSI strength equations underestimate the confined strength of highly interlocked and non-persistently jointed rock ***,the GSI strength equations are found to be valid to estimate the confined strength of persistently jointed rock masses with smooth and non-dilatant joint surfaces.

关键词： Rock mass strength Degree of interlock Non-persistently jointed rock mass Blocky rock mass Geological strength index(GSI) GSI strength equations

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Deformation-based support design for highly stressed ground with a focus on rockburst damage mitigation

引用

Journal of Rock Mechanics and Geotechnical Engineering 2022年第1期14卷 50-66页

作者： P.K.kaiser A.Moss Laurentian University SudburyONP3E 2C6Canada GeoK Inc.680 Ramsey Lake Road SudburyONP3E 6H5Canada Sonal Mining Technology Inc. 2488 Shadbolt LaneWest VancouverBCV7S 3J1Canada

As mines go deeper,mine designs become more fragile and effective rock support becomes a strategic element for ground control to facilitate timely construction and cost-effective access for uninterrupted *** article focuses on the design of integrated support systems for brittle ground when large displacements due to gradual bulking of stress-fractured rock or sudden violent bulking during rockbursts are induced by static and dynamic *** provides an overview of support design principles for a rational approach to ground control in deep mines when large deformations are anticipated near *** designs must not only account for load equilibrium but also for deformation *** importantly,the design approach must account for the fact that the support’s displacement capacity is being consumed as it is deformed after support *** is therefore necessary to design for the remnant support capacity,*** capacity remaining when the support is ***,if the support capacity can be consumed,it can also be restored by means of preventive support maintenance(PSM).The PSM concept for cost-effective ground control is introduced and illustrated by quantitative and operational *** to other design approaches,the deformation-based support design(DBSD)approach provides the capacity of an integrated support system as a function of imposed *** in this support capacity due to mininginduced deformation renders excavations increasingly more vulnerable if located within the influence of active mining and seismic *** deformation measurements are robust indicators of the decay in support capacity,scanning and other displacement monitoring technologies enable measurements to verify the DBSD approach,to assess the remnant safety margin of the deformed support,and to make operational support maintenance decisions.

关键词： Deep mining Deformation-based support design(DBSD) Cost-effective support Support consumption Preventive support maintenance(PSM)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：