检索结果-南通市图书馆

Genetic diversity and population structure analysis of Pistacia species revealed by phenylalanine ammonia-lyase gene markers and implications for conservation

引用

Journal of Forestry Research 2018年第4期29卷 986-996页

作者： Setareh Mirzavand karim sorkheh Mohammad Reza Siahpoosh Department of Agronomy and Plant Breeding Faculty of AgricultureShahid Chamran University of Ahvaz

Information on population genetic structure and crop genetic diversity is important for genetically improving crop species and conserving threatened species. The PAL gene sequence is part of a multigene family that can be utilized to design DNA marker systems for genetic diversity and population structure investigation. In the current study, genetic diversity and population structure of 100 accessions of wild Pistacia species were investigated with 78 PAL markers. A protocol for using PAL sequences as DNA markers was developed. A total of 313 PAL loci were recognized, showing 100% polymorphism for PAL markers. The PAL markers produced relatively more observed and effective alleles in Pistacia falcata and Pistacia atlantica, with a higher Shannon＇s information index and expected heterozygosity in P. atlantica, Pistacia vera and Pistacia mutica. Pairwise assessment of Nei＇s genetic distance and genetic identity between populations revealed a close association between geographically iso- lated populations of Pistacia khinjuk and Pistacia chinensis. The accessions of wild Pistacia species had more genetic relationship among studied groups of species. Analysis of molecular variance indicated 19% among- population variation and 81% within-population variation for the PAL gene based DNA marker. Population structure analysis based on PAL revealed four groups with high genetic admixture among populations. The results establish PAL markers as a functional DNA marker system and provide important genetic information about accessions from wild populations of Pistacia species.

关键词： Genetic relationship Classification Geneticresources PAL Phenylalanine ammonia-lyase gene

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Novel in silico EST-SSR markers and bioinformatic approaches to detect genetic variation among peach(Prunus persica L.)germplasm

引用

Journal of Forestry Research 2020年第4期31卷 1359-1370页

作者： Mehrana Koohi Dehkordi Tayebeh Beigzadeh karim sorkheh Department of Agronomy Faculty of AgriculturePayame-Noor UniversityP.O.Box 19395-3697TehranIran Department of Agronomy and Plant Breeding Faculty of AgricultureShahid Chamran University of AhvazP.O.Box 61355/144AhvazIran

Because there are thousands of peach cultivars,cultivar classification is a critical step before starting a breeding *** molecular markers such as simple sequence repeats(SSRs)can be *** this study,67 polymorphic primers produced 302 *** values for SI index(1.903)suggested higher genetic variability in the genotype under *** values for observed alleles(Na),expected heterozygosity(He),effective alleles(Ne),Nei’s information index(h),and polymorphic information content(PIC)were 4.5,0.83,5.45,0.83,and 0.81,*** dendrogram constructed based on Jaccard’s similarity coefficients outlined four distinct clusters in the entire *** addition,an analysis of molecular variance(AMOVA)showed that70.68%of the total variation was due to within-population variation,while 29.32%was due to variation among *** to this research,all primers were successfully used for the peach *** EST-SSR markers should be useful in peach breeding programs and other research.

关键词： Expressed sequenced tags(EST) Simple sequence repeats(SSR) Prunus persica L. Genetic diversityl

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Evaluation of seed quality and oil parameters in native Iranian almond(Prunus ***.) species

引用

Journal of Forestry Research 2015年第1期26卷 115-122页

作者： Soghra Kiani Shakiba Rajabpoor karim sorkheh Sezai Ercisli Department of Agriculture Payame Noor University Department of Biology Payame Noor University Department of Agronomy and Plant Breeding Faculty of Agriculture Shahid Chamran University of Ahvaz Department of Horticulture Agricultural FacultyAtaturk University

We assessed chemical composition and variation in oil content and seed weight of 40 wild-growing almonds（Prunus L. spp.） accessions collected from different parts of Iran. There were significant differences in kernel weight and oil parameters. Accessions ranged from0.20 to 1.5 g in kernel weight, 0.2–3.0 mm in shell thickness, and 16–55 % in oil content. The predominant vegetable oil components of kernels were 4.6–9.5 % palmitic acid, 0.4–0.8 % palmitoleic acid, 1.0–3.4 % stearic acid,48.8–88.4 % oleic acid and 11.3–33.2 % linoleic *** acid was detected in 15 accessions. High heritability was recorded for all studied traits and was maximum for shell thickness（98.5 %） and minimum for oil content（97.1 %）. Maximum and minimum ‘Euclidean＇pair wise dissimilarities were 17.9 and 0.5, *** 40 accessions were grouped into two major clusters.

关键词： Genetic diversity Kernel quality Oil parameters Wild almond(Prunus L.spp.)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Changes induced by osmotic stress in the morphology, biochemistry, physiology, anatomy and stomatal parameters of almond species(Prunus L. spp.) grown in vitro

引用

Journal of Forestry Research 2014年第3期25卷 523-534页

作者： Shakiba Rajabpoor Soghra Kiani karim sorkheh Farahnaz Tavakoli Department of Biology Payam e Noor University Department of Agriculture Payam e Noor University Department of Agronomy and Plant Breeding Faculty of Agriculture Shahid Chamran University of Ahwaz Department of Plant Breeding and Biotechnology Faculty of Agriculture Shahrekord University

We investigated the influence of different levels of osmotic stress on growth and development in selected wild almond species （eight Prunus spp.） grown in vitro. The study, while endorsing the efficacy of in vitro screening of auxiliary buds of wild almond for osmotic stress tolerance, showed species variability in its response to osmotic stress. Osmotic stress reduced growth and development of all the species. How-ever, the putative tolerant Prunus spp. showed better performance than the putative susceptible genotypes. On average there was an 80% de-crease in shoot dry weight at -1.2 MPa. Reduction in shoot weight was more common in osmotic stress-susceptible species in the section labeled‘Euamygdalus’. The tolerant Prunus species produced smaller changes in biochemical responses than the sensitive cultivars for malondialdehyde content, catalase activity, relative permeability of protoplast membranes, and net photosynthetic rate. The tolerant species maintained cell integrity better than drought sensitive species. Wild almond species in the section labeled ‘Spartioides’ （Prunus arabica （Olivier） Neikle, Prunus glauca＆amp;nbsp;（Browicz） A.E. Murray, Prunus scoparia Spach） and ‘Lycioides’ （Pru-nus lycioides Spach, Prunus reuteri Bossi. et Bushe） were best adapted to osmotic stress. Increase in chlorophyll concentration and leaf thickness under high osmotic stress can be considered as preliminary selection parameters for osmotic stress tolerance in Prunus spp. The study con-firmed the efficacy of the in vitro method for screening of large number of genotypes for osmotic stress tolerance in wild almond species.

关键词： Prunus spp. proline osmotic stress leaf character malon-dialdehyde protoplast membrane permeability

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Genetic diversity and phylogenetic relationships between and within wild Pistacia species populations and implications for its conservation

引用

Journal of Forestry Research 2016年第3期27卷 685-697页

作者： Parvaneh Iranjo Daryuoosh Nabati Ahmadi karim sorkheh Hamid Rajabi Memeari Sezai Ercisli Department of Agronomy and Plant Breeding Faculty of Agriculture Shahid Chamran University of Ahwaz Department of Horticulture Agricultural Faculty Ataturk University

Although cultivation and utilization of Pistacia are fully exploited, the evolutionary history of the Pistacia genus and the relationships among the species and accessions is still not well understood. The aim of this study was to analyze random amplified polymorphic DNA（RAPD） in a total of 50 accessions of wild pistachio species, which included five populations Pistacia vera, Pistacia khinjuk,Pistacia atlantica, Pistacia mutica, and Pistacia *** levels of genetic diversity were detected within wild pistachio accessions, as revealed by using the unweighted pair-group method with arithmetic averaging and supported via analysis of molecular variance. The objectives of this investigation were to estimate marker indices, polymorphic information contents（PICs）, and genetic similarities（GS）for RAPD markers; assess the genetic diversity of Pistacia species, using GS estimated from RAPD fingerprints and molecular characterization; and facilitate the use of markers in inter-specific introgression and cultivar *** of the 149 polymerase chain reaction fragments that were scored, 146（97.98 %） were polymorphic. Genetic similarities ranged from 0.3 to 0.86 %, marker indices ranged from 2.98 to 17.74 %, and PICs ranged from 0.80 to0.99 %. Our results provided great molecular identification of all assayed genotypes, which have shown that there is large quantity of genetic diversity among the pistachio accessions. This finding might render striking information in breeding management strategies for genetic conservation and cultivar development.

关键词： Pistachio Pistacia Phylogenetic RAPD Population structure Genetic diversity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Discriminating ability of molecular markers and morphological characterization in the establishment of genetic relationships in cultivated genotypes of almond and related wild species

引用

Journal of Forestry Research 2009年第3期20卷 183-194,285页

作者： karim sorkheh Behrouz Shiran Soghra Kiani Nazanin Amirbakhtiar Sadegh Mousavi Vahid Rouhi Shahram Mohammady-D Thomas M.Gradziel Lyudmyla V.Malysheva-Otto Pedro Martínez-Gómez Department of Agronomy and Plant Breeding Faculty of Agriculture Shahrekord University Scientific assistance of Payam-e-Nour University Department of Plant Science University of California Institute of Plant Genetics and Crop Plant Research (IPK) Department of Plant Breeding

A total 23 morphological traits, 19 AFLP-primer combinations, 80 RAPD primers and 32 SSR primer pair were used to compare the informativeness and efficiency of random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers in establishing genetic relationships among 29 almond cultivars and three related wild species. SSRs presented a high level of polymorphism and greater information content, as assessed by the expected hetrozygosity, compared to AFLPs and RAPDs. The lowest values of expected hetrozygosity were obtained for AFLPs; however AFLPs showed the highest efficiency, owing to their capacity to reveal large numbers of bands per reaction, which led to high values for various types of indices of diversity. All the three techniques discriminated almond genotypes very effectively, except that SSRs failed to discriminate between ‘Monagha’ and ‘Sefied’ almond genotypes. The correlation coefficients of similarity were statistically significant for all the three marker systems, but were lower for the SSR data than for RAPDs and AFLPs. For all the markers, high similarity in dendrogram topologies was obtained, although some differences were observed. All the dendrograms, including that obtained by the combined use of all the marker data, reflect relationships for most of cultivars according to their geographic diffusion. AMOVA detected more variation among cultivated and related wild species of almond within each geographic group. Bootstrap analysis revealed that the number of markers used was sufficient for reliable estimation of genetic similarity and for meaningful comparisons of marker types.

关键词： Amplified Fragment Length Polymorphisms (AFLPs) Random Amplified Polymorphic DNA (RAPDs) Simple-Sequence Repeats (SSRs) germplasm genetic relationships breeding prunus dulcis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：