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Effects of photoperiod and temperature on diapause induction in Conogethes punctiferalis （Lepidoptera： Pyralidae）

Insect Science 2014年第5期21卷 556-563页

作者： Li-Rong Xu Xinzhi Ni Zhen-Ying Wang kang-lai he The State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences China USDA-ARS Crop Genetics and Breeding Research Unit University of Georgia-Tifton Campus Tifton GeorgiaUSA

The yellow peach moth, Conogethes punctiferalis （Guenee）, a multivoltine species that overwinters as diapausing larvae, is one of the most serious insect pests on maize in China. Effect of photoperiod and temperature on larval diapause was examined under empirical laboratory conditions. Short-day treatments caused larval diapause at 25℃, and the critical photoperiod was between 12 and 13 h （or 12 h 51 min） light per day. No sensitive instar was identified for diapause induction under alternated short- （L ： D 11 ： 13 h） and long-day （L ： D 14 ： 10 h） treatments at different larval stages. However, accumulative treatment of three instars and 10 d under short-day treatment was required for the induction of 50% larval diapause. All larvae entered diapause at 20℃, whereas less than 3% did so at 30℃, irrespective of the long- or short-day treatment. Furthermore, under the short-day treatment, more than 90% of larvae went into diapause with temperatures ≤ 25℃, but less than 17% did so at 28℃. In contrast, under the long-day treatment, less than 19% of larvae went into diapause with temperatures ≥23 ℃. The forward shift （5℃） of critical temperature under the long-day regime demonstrated the compensatory effect of temperature and photoperiod on diapause induction. In conclusion, C. punctiferalis had a temperature-dependent type Ⅰ photoperiodic diapause response; there was no sensitive instar for diapause determination, but the photoperiodic accumulation time countermeasures both of the short-day cycles and the number ofinstars exposed, and the photoperiodic diapause response, was a temperature-compensated phenomenon.

关键词： Conogethes punctiferalis diapause induction photoperiod sensitive larval instar temperature dependence yellow peach moth

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Does Bt maize expressing CrylAc protein have adverse effects on the parasitoid Macrocentrus cingulum （Hymenoptera： Braconidae）？

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Insect Science 2017年第4期24卷 599-612页

作者： Zeng-Xia Wang Yun-he Li kang-lai he Shu-Xiong Bai Tian-Tao Zhang Wan-Zhi Cai Zhen-Ying Wang State Key Laboratory for Biology of Plant Diseases and Insect Pests MOA - CABI Joint Laboratory for Bio-safety Institute of Plant Protection Chinese Academy of Agricultural Science Beijing China Department of Entomology China Agriculture University Beijing China

The potential effects of insect-resistant, genetically engineered （GE） crops on non-target organisms, especially on predators and parasitoids, must be evaluated before their commercial cultivation. The effects of GE maize that produces CrylAc toxin on the parasitoid Macrocentrus cingulum were assessed by direct bioassay and indirect bioassay. In the indirect bioassay, parasitism rate, cocoon weight and the number of M. cingu- lure progeny produced per host were significantly reduced when M. cingulum-parasitized CrylAc-susceptible Ostriniafurnacalis were fed a diet containing purified CrylAc; how- ever, life-table parameters of M. cingulum were not adversely affected when the same assay was performed with Cry 1Ac-resistant （9. furnacalis. These results indicated that the detrimental effects detected with a CrylAc-susceptible host were mediated by poor host quality. In a direct bioassay, no difference in life-table parameters were detected when M. cingulum adults were directly fed a 20% honey solution with or without CrylAc; however, survival and longevity were significantly reduced when M. cingulum adults were fed a honey solution containing potassium arsenate, which was used as a positive control. The stability and bioactivity of CrylAc toxin in the food sources and CrylAc toxin uptake by the host insect and parasitoid were confirmed by enzyme-linked immunosorbent assay and sensitive-insect bioassays. Our results demonstrate that M. cingulum is not sensitive to CrylAc toxin at concentrations exceeding those encountered in Bacillus thuringiensis maize fields. This study also demonstrates the power of using resistant hosts when assess- ing the risk of genetically modified plants on non-target organisms and will be useful for assessing other non-target impacts.

关键词： CrylAc toxin ELISA environmental risk assessment non-target effects parasitoids prey/host quality-mediated effects

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Comparison of larval performance and oviposition preference of Spodoptera frugiperda among three host plants:Potential risks to potato and tobacco crops

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Insect Science 2021年第3期28卷 602-610页

作者： Jing-Fei Guo Meng-Di Zhang Zu-Peng Gao Deng-Jie Wang kang-lai he Zhen-Ying Wang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingChina Engineering Research Center of Natural Enemy Insects/Institute of Biological Control Jilin Agricultural UniversityChangchunChina Mianyang Academy of Agricultural Sciences MianyangSichuan ProvinceChina

Fall armyworm(FAW),Spodoptera frugiperda(***),is a majorpolyphagous pest with the potential to seriously damage various crops.A better under-standing of FAW's performance on different hosts may help to predict which plants willbe attacked when the preferred host is absent,and facilitate the development of effectivepest management *** compared the larval performance of FAW fed on maizewith that of FAW fed on potato and tobacco,which are important crops in China,usingan age-stage two-sex life table and adult female oviposition preference *** experiments with potato,tobacco,or maize as the host,FAW reared on maize exhib-ited the strongest performance with shorter developmental time in thc larval stage,longerlongevity,and a higher reproductive rate in *** oviposited on maize in pref-erence to potato or *** with larvae fed on maize,those fed on potato andtobacco exhibited significantly lower survival,with only 31.61%and 8.13%developing tothe adult stage,*** life table parameters,including the mean generationtime(T),net reproductive rate(R^(0)),finite rate of increase(λ),and intrinsic rate of nat-ural increase(r)were negatively affected in FAW fed on potato and *** resultssupport the preference-performance hypothesis,that is,that herbivore females maximizefitness by choosing host plants associated with strong larval *** lar-vae and adults performed poorly on potato and tobacco,their offspring will be capable ofcstablishing populations on them,posing a potential threat to these crops in China.

关键词： fall armyworm host suitability maize potato tobacco

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Initial detections and spread of invasive Spodoptera frugiperda in China and comparisons with other noctuid larvae in cornfields using molecular techniques

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Insect Science 2020年第4期27卷 780-790页

作者： Da-Peng Jing Jing-Fei Guo Yu-Ying Jiang Jian-Zhou Zhao Amit Sethi kang-lai he Zhen-Ying Wang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingChina China National AgroTech Extension and Service Center BeijingChina Corteva Agriscience JohnstonIAUSA

The fall armyworm,Spodoptera frugiperda,is a species native to the Americas and has spread to many countries in Africa and Asia in recent *** actions for potential invasion of *** to China coordinated by government agencies and agricultural extension systems resulted in timely detection in January 2019 in Yunnan province neighboring onto *** extensive monitoring in southern provinces of China since February 2019 resulted in dynamic tracking of *** spreading to 13 provincial regions in China within 4 months by May 10,2019,which is crucial for timely management actions in the *** first detections of ***(corn strain)in China were confirmed using cytochrome oxidase subunit 1(COl)and triosephosphate isomerase(Tpi)genes molecular marker *** addition to ***,larvae of three other noctuid species with similar morphological appearance(***,*** and Mythimna separata)can occur simultaneously and cause similar damage in cornfields in southern ***,we can use both morphological and molecular marker methods to compare larval stages of four noctuid ***,we discuss the risk of potential spread of invasive *** to other regions and impact on corn production in China.

关键词： com molecular identification Mythimna separata,Spodoptera exigua Spodoptera frugiperda',Spodoptera litura

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Seasonal and geographical variation in diapause and cold hardiness of the Asian corn borer, Ostrinia furnacalis

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Insect Science 2015年第4期22卷 578-586页

作者： Hai-Cui Xie Dun-Song Li Hong-Gang Zhang Charles E. Mason Zhen-Ying Wang Xin Lu Wan-Zhi Cai kang-lai he The State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 Department of Entomology China Agriculture University Beijing 100193 The Plant Protection Research Institute Guangdong Academy of Agricultural Sciences Guangzhou 510640 China Department of Entomology and Wildlife Ecology University of Delaware 531 S College Ave Rm 250 Newark DE 19716-2160 USA Institute of Plant Protection Jilin Academy of Agricultural Sciences Gongzhuling 136100 Jilin China

Asian corn borer, Ostrinia furnacalis （Guenee）, is a key corn pest in the Asian-Western Pacific countries. It overwinters as flail-grown larvae in plant stalks or in a spun-silk covering located in the plant debris in the temperate regions of China. Supercooling point （SCP） and survival rate after low sub-zero temperature treatment were assessed for field-collected populations in the laboratory using a cool bath with a 1 ℃/min cooling rate until -40℃. Mean SCPs were varied among geographical populations, with a significant decline from -22.7℃ of Haikou, the multivoltine tropical population in the south, to -28.5℃ of Gongzhuling, the univoltine temperate population in the northeast of China. In addition, there was more than 1℃ difference in SCP between Gongzhuling univoltine and bivoltine populations that were from the same geographic origin. Mean SCPs of the Guangzhou population fluctuated over the year, with significantly lower SCPs in winter than in other seasons, which correlated with a significantly higher proportion of diapausing larvae in winter than in other seasons. Over 41% of overwintering larvae from the northeast population could withstand to be supercooled for a few minutes to the low sub-zero temperature of-40℃, but only 6.7% of their southern counterparts did so. The findings from this study suggest that O. furnacalis mostly takes advantage of freeze avoidance as diapausing larvae for overwintering in the southern region, whereas it exhibits freeze tolerance in diapause in the northeastern region.

关键词： cold hardiness diapause geographical population Ostriniafurnacalis overwintering seasonality

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Regulation of insect behavior by non-coding RNAs

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Science China(Life Sciences) 2024年第6期67卷 1106-1118页

作者： Jing he Le kang State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of ZoologyChinese Academy of SciencesBeijing 100101China Beijing Institutes of Life Sciences Chinese Academy of SciencesBeijing 100101China College of Life Science Hebei UniversityBaoding 071002China

The adaptation of insects to environments relies on a sophisticated set of behaviors controlled by molecular and physiological *** the past several decades,accumulating studies have unveiled the roles of non-coding RNAs(nc RNAs)in regulating insect *** RNAs assume particularly pivotal roles in the behavioral plasticity of insects by rapidly responding to environmental *** RNAs also contribute to the maintenance of homeostasis of insects by fine-tuning the expression of target ***,a comprehensive review of nc RNAs'roles in regulating insect behaviors has yet to be ***,we present the recent progress in our understanding of how nc RNAs regulate various insect behaviors,including flight and movement,social behavior,reproduction,learning and memory,and *** refine the intricate mechanisms by which nc RNAs modulate the function of neural,motor,reproductive,and other physiological systems,as well as gene expression in insects like fruit flies,social insects,locusts,and ***,we discuss potential avenues for future studies in nc RNA-mediated insect behaviors.

关键词： ncRNAs insect behavior plasticity fine-tuning environmental adaptation

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Dual-functional poly(2-methyl-2-oxazoline)/poly(2-(dimethylamine)ethyl methacrylate)mixed brushes with switchable protein adsorption and antibacterial properties

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Chinese Journal of Chemical Engineering 2024年第2期66卷 19-30页

作者： kang he Liangyu Zhu Yanmei Wang CAS Key Laboratory of Soft Matter Chemistry Department of Polymer Science and EngineeringUniversity of Science and Technology of ChinaHefei 230026China

herein,binary mixed brushes consisting of poly(2-methyl-2-oxazoline)(PMOXA)and poly(2-(dimethylamine)ethyl methacrylate)(PDMAEMA)with different chain lengths were fabricated by successive grafting of NH_(2)-terminated PMOXA and SH-terminated PDMAEMA onto polydopamine-anchored *** mixed-brush coating was characterized by variable-angle spectroscopic ellipsometry,X-ray photoelectron spectroscopy,Fourier transform infrared spectroscopy,zeta potential measurements,water contact angle,and atomic force *** mixed brushes showed tunable surface charge,wettability,and surface roughness,depending on the degree of PDMAEMA swelling under varying pH and ionic strength(Ⅰ).Then the adsorption behaviors of pepsin,bovine serum albumin(BSA),γ-globulin,and lysozyme,four very different proteins with regard to isoelectric point,on the mixed brushes coating were studied by using fluorescence microscopy and surface plasmon *** the chain length of PDMAEMA was about twice as long as PMOXA,the mixed brushes not only had high adsorption capacity for pepsin,BSA,and y-globulin but also had a desorption efficiency of 86.9%,87.1%,and 93.5%,*** is explained that electrostatic attraction between the protonated PDMAEMA and positively charged acidic proteins(pepsin and BSA,whose isoelectric points were below the pK_(a) of PDMAEMA)would drive the intensive adsorption(at pH 3,I=10^(-3)mol·L^(-1)for pepsin,and pH 5,I=10^(-5)mol·L^(-1)for BSA),while desorption was dominated by the hydrophilic PMOXA when PDMAEMA was shrinking(at pH 7,I=10^(-1)mol·L^(-1)for pepsin,and pH 9,I=10^(-1)mol·L^(-1)for BSA).Furthermore,the isoelectric precipitation led to the adsorption of neutral protein(γ-globulin,whose isoelectric point was near the pK_a of PDMAEMA)at pH 7,I=10^(-5)mol·L^(-1),while electrostatic repulsion and antifouling PMOXA triggered the desorption of y-globulin at pH 3,I-10^(-1)mol·L^(-1).However,alkaline protein(lysozyme,whose isoelectric point was higher than

关键词： Switchable conformation transition Controllable protein adsorption Mixed brushes Isoelectric point Antibacterial surface

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Tribological Behaviors of Electroless Nickel-Boron Coating on Titanium Alloy Surface

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Chinese Journal of Mechanical Engineering 2024年第1期37卷 309-320页

作者： Yao Jia Jianping lai Jiaxin Yu Huimin Qi Yafeng Zhang Hongtu he Key Laboratory of Testing Technology for Manufacturing Process in Ministry of Education State Key Laboratory of Environment-Friendly Energy MaterialsSouthwest University of Science and TechnologyMianyang 621010China

Titanium alloys are excellent structural materials in engineering fields,but their poor tribological properties limit their further *** plating is an effective method to enhance the tribological performance of alloys,but it is difficult to efficiently apply to titanium alloys,due to titanium alloy’s strong chemical *** this work,the electroless Nickel-Boron(Ni-B)coating was successfully deposited on the surface of titanium alloy(Ti-6AL-4V)via a new pre-treatment ***,linearly reciprocating sliding wear tests were performed to evaluate the tribological behaviors of titanium alloy and its electroless Ni-B *** was found that the Ni-B coatings can decrease the wear rate of the titanium alloy from 19.89×10^(−3)mm^(3)to 0.41×10^(−3)mm^(3),which attributes to the much higher hardness of Ni-B *** heat treatment,the hardness of Ni-B coating further increases corresponding to coating crystallization and hard phase ***,heat treatment does not improve the tribological performance of Ni-B coating,due to the fact that higher brittleness and more severe oxidative wear exacerbate the damage of heat-treated ***,the Ni-B coatings heat-treated both in air and nitrogen almost present the same tribological *** finding of this work on electroless coating would further extend the practical applications of titanium alloys in the engineering fields.

关键词： Electroless coating Titanium alloy Tribology Wear heat treatment Nanoindentation

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Machine learning and neural network supported state of health simulation and forecasting model for lithium-ion battery

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Frontiers in Energy 2024年第2期18卷 223-240页

作者： Nan QI kang YAN Yajuan YU Rui LI Rong HUANG lai CheN Yuefeng SU Beijing Institute of Technology Chongqing Innovation Center Chongqing 401120China Department of Energy and Environmental Materials School of Materials Science and EngineeringBeijing Institute of TechnologyBeijing 100081China Beijing Electric Vehicle Co. Ltd.Beijing 100176China

As the intersection of disciplines deepens,the field of battery modeling is increasingly employing various artificial intelligence(AI)approaches to improve the efficiency of battery management and enhance the stability and reliability of battery *** paper reviews the value of AI methods in lithium-ion battery health management and in particular analyses the application of machine learning(ML),one of the many branches of AI,to lithium-ion battery state of health(SOH),focusing on the advantages and strengths of neural network(NN)methods in ML for lithium-ion battery SOH simulation and *** is one of the important branches of ML,in which the application of NNs such as backpropagation NN,convolutional NN,and long short-term memory NN in SOH estimation of lithium-ion batteries has received wide *** so far have shown that the utilization of NN to model the SOH of lithium-ion batteries has the advantages of high efficiency,low energy consumption,high robustness,and scalable *** the future,NN can make a greater contribution to lithium-ion battery management by,first,utilizing more field data to play a more practical role in health feature screening and model building,and second,by enhancing the intelligent screening and combination of battery parameters to characterize the actual lithium-ion battery SOH to a greater *** in-depth application of NN in lithium-ion battery SOH will certainly further enhance the science,reliability,stability,and robustness of lithium-ion battery management.

关键词： machine learning lithium-ion battery state of health neural network artificial intelligence

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CFD-DEM study on the synergistic effect of coke consumption and particle dynamics in the blast furnace raceway

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Particuology 2024年第6期89卷 270-278页

作者： Peng Xu Peng Han Zhijun he Xizhong An kang Sun Lihua Gao Key Laboratory of Green Low-Carbon and Intelligent Metallurgy School of Materials and MetallurgyUniversity of Science and Technology LiaoningAnshan 114000China Key Laboratory for Ecological Metallurgy of Multimetallic Mineral of Ministry of Education School of MetallurgyNortheastern UniversityShenyang110819China

In this study,the gas-solid flow process in the blast furnace raceway is numerically simulated using coupled computational fluid dynamics and the discrete element method(CFD-DEM).The coke reaction kinetics data are imported into the DEM model to reproduce the consumption process of each coke *** effects of inlet gas velocity and angle on the morphology of the raceway,coke consumption rate,coke bed temperature,and particle size distribution in the blast process are systematically investigated and *** results show that the consumption of coke particles promotes the formation of raceways during the blast *** the same time,a coke mixture layer is produced at the edge of the *** higher the inlet gas velocity,the thicker the coke mixture layer in the middle and upper parts of the raceway region,and the larger the proportion of small particles in the coke mixture *** effect of the inlet gas angle on the raceway region is less than the inlet gas ***,with the increase in the inlet gas angle,the high-temperature region of the coke bed extends downward gradually,which is conducive to activating the hearth.

关键词： Blast furnace Raceway Multiphase flow CFD-DEM Modeling

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