检索结果-南通市图书馆

Chinese Medical Journal 2019年第12期132卷 1478-1481页

作者： U Po lam Edmundo Patricio Lopes Lao kan Chit lam Mario Evora Na-Qiong Wu Department of Cardiology Centro Hospital Conde de Sao JanuarioMacaoChina University of Michigan Ann ArborMichigan 48104USA Department of Cardiology Center of Endocrinology & CardiometabolismNational Center for Cardiovascular DiseasesFuwai HospitalBeijing 100037China

Compared with trans-femoral artery access (TFA), trans-radial artery access (TRA) for percutaneous coronary interventions (PCI) has been shown to significantly reduce 30-day mortality, in-hospital major adverse cardiac and cardiovascular events (a composite of 30-day mortality and in-hospital myocardial re-infarction, target vessel revascularization, and cerebrovascular events), major bleeding, and access site complications.[1] TRA is more easily compressible, minimizing hematoma risk, and lower crossover rates to another access. Patients may ambulate immediately after procedure, that hospitalization can be shortened significantly. In some centers, patient with TRA can be discharged on the same day of procedure. It is also superior to TFA with closure devices, and becomes the preferred access site for recent PCI.[2]

关键词： Trans-brachial artery access coronary artery procedures

Anaerobic bacteria and intrahepatic stones: detections of Clostridium sp. and Bacteroides fragilis

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2000年第9期113卷 90-93页

作者： LIU Yanlei kan lam Yajarayma J Tang Paul H Gumerlock Dong Ki Lee Myung Hwa Kim Sum P Lee Joseph SilvaJr Joseph W Leung Division of Gastroenterology and Mr & MrsCWLaw Biofilm Research Center University of CaliforniaDavis MedicalCenterSacramentoCA95817USA

To detect anaerobic bacteria Clostridium sp . and Bacteroides fragilis in intrahepatic stones by molecular genetic method Methods DNA was extracted from 59 stone samples and subjected to polymerase chain reaction (PCR) amplification targeting the 16S rRNA gene of Clostridium sp . and the glutamine synthetase gene of Bacteroides fragilis Single-strand conformational polymorphism (SSCP) analysis was performed to identify the Clostridium sp Results 16S rRNA gene sequences for Clostridium sp. were identified in 49 stones (83%, 49/59) The two most common groups were detected in 19 (41%) and 17 (37%) of the 46 samples using SSPC analysis, and 25/59 (42%) stones were tested positive for Bacteroides fragilis Conclusions Anaerobes such as Clostridium sp and Bacteroides fragilis present in intrahepatic stones and may play a role in stone formation PCR is a useful technique to detect fastidious pathogens, which are difficult to culture SSCP of PCR products is a rapid method in differentiating bacterial species

关键词： intrahepatic stone anaerobic bacteria PCR single-strand confromational polymorphism

Microstructure and Internal Friction Behavior of Laser 3D Printed Fe-Based Amorphous Composites

在线全文

学校读者我要写书评

暂无评论

Acta Metallurgica Sinica(English Letters) 2024年第1期37卷 196-204页

作者： Shuilong Huang Qingjun Chen Li Ji kan Wang Guosheng Huang School of Material Science and Engineering Nanchang Hangkong UniversityNanchang330063China State Key Laboratory for Marine Corrosion and Protection Luoyang Ship Material Research Institute(LSMRI)Qingdao266237China

Laser 3D printing,also known as laser additive manufacturing(lam),is favored for its ability to form bulk metallic glass(BMG)and its composite materials(BMGcs)with freeform *** this work,two different kinds of Fe_(41)Co_(7)Cr_(15)Mo_(14)C_(15)B_(6)Y_(2)amorphous coatings(A and B)were prepared by using lam technology under air-and water-cooled conditions,respectively;meanwhile,to reduce the cracks generated due to the residual thermal stresses,coating C obtained by air-sweep annealing of B with a low energy-density *** morphology and amorphous content and microstructure of the coatings were investigated,the results show many cracks in coating B deposited under water-cooled conditions,and its microstructure shows an amorphous-crystal-nanocrystalline mixed *** was suppressed in coating C,obtained by air-sweep annealing based on coating B,but the amorphous content was reduced from 32.6 to 13.4%.And the hardness and corrosion resistance of the coating will increase with the increase in the amorphous ***,the internal friction behavior of a BMGcs was prepared on the basis of the process of sample C is compared with that of as-cast amorphous *** results show that the low temperature internal friction behavior of BMGcs is affected by the defects produced during printing,and the high temperature internal friction behavior is affected by the precipitated hard phase.

关键词： Laser 3D printing Amorphous coating Microstructure Corrosion resistance Internal friction behavior

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

香港经济转型与税收改革研究

香港经济转型与税收改革研究

作者：林拾根（lam SAP kan）华侨大学

学位级别：硕士

香港99年财赤预算高达数百亿。政府提出当务之急是解决财政赤字,否则会阻碍经济复苏的机会。同时敬告市民需要共同承担政府财赤的责任。说明了未来加税无可避免的事,有急切进行税制改革,开徴以开支为本的“消费税”项。香港经济正处于... 详细信息

香港99年财赤预算高达数百亿。政府提出当务之急是解决财政赤字,否则会阻碍经济复苏的机会。同时敬告市民需要共同承担政府财赤的责任。说明了未来加税无可避免的事,有急切进行税制改革,开徴以开支为本的“消费税”项。香港经济正处于低迷和转型期间,假若港府在缺乏理据和充足谘询,仓卒推行开徵“消费税”,只会造成经济的苦况雪上加霜,严重甚至引起市民不满和经济问题政治化。香港财政赤字的成因,来源于政府内部失控的开支和依重非经常性卖地的收益。港府开源考虑增加税收,首应研究现有税制如何把税基扩阔,采取简单和可行的方法增加税收。香港企业所得税的徴税原则[利润地域来源地],建议将商贸结付过程加入懲税原则内,可以扩阔企业所得税税网和增加税收。同时,实施[用者自负]原则的使用塑胶袋的环保税,环保税不但增加库房收入兼具推动环保。港府节流减少开支方面,考虑相对较容易的“私营化”计划,将公共服务“私营化”以及公营公司上市,也可为财政增加收入和节省公务开支。港府提出税制改革非减灭财赤之良方,应该着重于经济发展方面,经济产业结构迅速顺利转型,失业问题解决和企业利润提高,税收必然随着经济复苏增加,公共财赤自然相应地减少。自80年代制造业的不断北移,香港经济产业结构已了形成[工业空心]。香港要继续保持”比较优势”和当今世界工业发展的潮流,制造业实际面临的任务,是将劳动或资本密集型产业转变为资本和技术密集型产业。香港今后单方向发展服务业,则很可能在整体竞争力方面不及其他对手,反而动摇香港在金融业、港口运输服务等方面的优势地位。因此,香港未来经济采取”双轨式”的发展路向较具合理。香港的科技行业方面的发展起步较晚,这鉴于香港政府一直奉行[积极不干预]和缺乏长远的工业政策。香港经济产业结构转型成功,需要提出之“积极不干预”原则,应以客观情势变迁而予以修正,和掌握目前的优势和机遇。再将“积极不干预”作为金科玉律,不但既不符合正在变化中的事实,而且会妨碍正确地观察和处理香港经济出现的紧迫问题。

关键词：税收改革税制改革积极不干预产业结构转型企业所得税资本密集型产业工业制造经济发展港口运输当今世界工业

Peptidic defective interfering gene nanoparticles against Omicron,Delta SARS-CoV-2 variants and influenza A virus in vivo

同方学位论文库评论

在线全文

同方学位论文库

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2022年第9期7卷 3484-3493页

作者： Hanjun Zhao Chuyuan Zhang Hoiyan lam Xinjie Meng Zheng Peng Man Lung Yeung Jasper Fuk-Woo Chan Kelvin Kai-Wang To Kwok-Yung Yuen State Key Laboratory of Emerging Infectious Diseases Li Ka Shing Faculty of MedicineThe University of Hong KongHong Kong Special Administrative RegionChina Department of Microbiology School of Clinical MedicineLi Ka Shing Faculty of MedicineThe University of Hong KongHong Kong Special Administrative RegionChina Centre for Virology Vaccinology and TherapeuticsHong Kong Science and Technology ParkHong Kong Special Administrative RegionChina Department of Infectious Disease and Microbiology The University of Hong Kong-Shenzhen HospitalShenzhenGuangdong ProvinceChina Carol Yu Centre for Infection The University of Hong KongPokfulamHong Kong Special Administrative RegionChina Guangzhou Laboratory GuangzhouGuangdong ProvinceChina

Defective interfering genes(DIGs)are short viral genomes and interfere with wild-type viral ***,we demonstrate that the new designed SARS-CoV-2 DIG(CD3600)can significantly inhibit the replication of SARS-CoV-2 including Alpha,Delta,Kappa and Omicron variants in human HK-2 cells and influenza DIG(PAD4)can significantly inhibit influenza virus replication in human A549 *** dose of influenza DIGs prophylactically protects 90%mice from lethal challenge of A(H1N1)pdm09 virus and CD3600 inhibits SARS-CoV-2 replication in hamster lungs when DIGs are administrated to lungs one day before viral *** further investigate the gene delivery vector in the respiratory tract,a peptidic TAT2-P1&LAH4,which can package genes to form small spherical nanoparticles with high endosomal escape ability,is demonstrated to dramatically increase gene expression in the lung ***2-P1&LAH4,with the dual-functional TAT2-P1(gene-delivery and antiviral),can deliver CD3600 to significantly inhibit the replication of Delta and Omicron SARS-CoV-2 in hamster *** peptide-based nanoparticle system can effectively transfect genes in lungs and deliver DIGs to inhibit SARS-CoV-2 variants and influenza virus in vivo,which provides the new insight into the drug delivery system for gene therapy against respiratory viruses.

关键词： influenza vivo Delta

自来水中的药物:中国人类健康风险评估及监测建议框架

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

环境与职业医学 2013年第10期30卷 773-773页

作者： Ho Wing Leung Ling Jin Si Wei Mirabelle Mei Po Tsui Bingsheng Zhou Liping Jiao Pak Chuen Cheung Yiu kan Chun Margaret Burkhardt Murphy Paul Kwan Sing lam

[背景]在世界范围内,已知药品会污染自来水,但是在中国尚无相关的人类健康风险评估。[目的]监测中国自来水中的32种药品,并评估暴露的终身人类健康风险,以便为未来的优先级管理和风险管理提供信息。[方法]作者分析来自13个城市的样本(n=... 详细信息

[背景]在世界范围内,已知药品会污染自来水,但是在中国尚无相关的人类健康风险评估。[目的]监测中国自来水中的32种药品,并评估暴露的终身人类健康风险,以便为未来的优先级管理和风险管理提供信息。[方法]作者分析来自13个城市的样本(n=113),并将检测到的浓度与现有或新得出的安全水平进行比较,以评估除产前期以外不同生命阶段的风险商数(RQs)。[结果]在89%的样本中检测到17种药品,大多数(92%)检出浓度<50ng/L。在≥20%的样本中检测到咖啡因(中等～最大浓度,24.4～564ng/L)、甲硝唑(1.8～19.3ng/L)、水杨酸(16.6～41.2ng/L)、氯贝酸(1.2～3.3ng/L)、卡马咪嗪(1.3～6.7ng/L)、二甲硝咪唑(6.9～14.7ng/L)。由于自来水中检测到药品水平及阳性频率均高,长江中下游地区城市和广州被视为受药品污染的重点区域。在检测到的17种药品中,13种为极低风险水平,但4种药品(二甲硝咪唑、甲砜霉素、磺胺二甲基嘧啶和克拉霉素)至少在一个生命阶段的RQ≥0.01,尤其是婴幼儿和儿童阶段,应被视为管理的高度优先级。本文提出了一个基于指标的监测框架,为中国的药品来源识别、水处理效果及水安全管理提供信息。[结论]中国的自来水是人体药品暴露另外的一个路径,特别是二甲硝咪唑,尽管基于当前的毒性数据,它对人体健康是低风险的。药品检测和监测建议框架可用于在中国和其他地方的水源保护和风险管理。

关键词：中国指标生命阶段药品风险管理自来水

在线全文

学校读者我要写书评

暂无评论

甘薯糖蛋白的免疫调节作用研究

西南农业大学学报（自然科学版） 2000年第3期22卷 257-260页

作者：阚建全阎磊陈宗道贺稚非王光慈西南农业大学食品科学学院重庆400716

初步研究了经DEAE5 2和SephadexG1 0 0柱层析纯化的北京 2号甘薯糖蛋白的免疫调节作用。结果表明甘薯糖蛋白浓度达 5 0 μg/ml时可促进PHA人外周血淋巴细胞转化 ,1 0 0 μg/ml或者 1 5 0 μg/ml时间可显著提高PHA刺激的人外周血淋巴细... 详细信息

初步研究了经DEAE5 2和SephadexG1 0 0柱层析纯化的北京 2号甘薯糖蛋白的免疫调节作用。结果表明甘薯糖蛋白浓度达 5 0 μg/ml时可促进PHA人外周血淋巴细胞转化 ,1 0 0 μg/ml或者 1 5 0 μg/ml时间可显著提高PHA刺激的人外周血淋巴细胞转化 ,刺激指数分别达到 6 .5和 4.5 (P <0 .0 5 )。腹腔注射甘薯糖蛋白 80mg/kg·d可促进小鼠腹腔巨噬细胞的吞噬功能 ,吞噬指数和吞噬百分数均高于共它组 (P <0 .0 5 ) ;另外 ,小鼠脾指数、胸腺指数的测定也得到同样的结果 ;小鼠脾脏、胸腺光镜和电镜下观察发现 ,随着甘薯糖蛋白剂量增加 ,脾淋巴小结增多扩大 ,胸腺T细胞线粒体增多。这些结果表明甘薯糖蛋白有明显的增强免疫调节的功能。

关键词：甘薯糖蛋白免疫调节功能综合利用保健食品

DNA引物酶抑制剂碘化-3,3′-二乙基-9-甲基-硫杂羰花青诱导人白血病HL-60细胞凋亡(英文)

在线全文

学校读者我要写书评

暂无评论

药学学报 2006年第10期41卷 978-984页

作者：李志铭姜文奇管忠震朱孝峰周军民谢冰芬冯公侃朱振宇刘宗潮中山大学肿瘤防治中心华南肿瘤学国家重点实验室广东广州510060

目的研究DNA引物酶抑制剂碘化-3,3′-二乙基-9-甲基-硫杂羰花青(DMTCC I)诱导人粒细胞性白血病HL-60细胞凋亡并探索其机制。方法分别采用不同浓度的DMTCC I处理培养于RPM I-1640培养基的HL-60细胞。采用MTT法检测DMTCC I对HL-60细胞的... 详细信息

目的研究DNA引物酶抑制剂碘化-3,3′-二乙基-9-甲基-硫杂羰花青(DMTCC I)诱导人粒细胞性白血病HL-60细胞凋亡并探索其机制。方法分别采用不同浓度的DMTCC I处理培养于RPM I-1640培养基的HL-60细胞。采用MTT法检测DMTCC I对HL-60细胞的生长抑制作用。采用流式细胞仪和DNA琼脂糖凝胶电泳方法检测细胞凋亡。采用蛋白免疫印迹(W estern b lotting)法观察凋亡相关蛋白survivin,Bc l-xL,Bad,Bax,Bc l-2,caspase-9,caspase-3,caspase-6,PARP,DFF45和lam in B的表达。采用ApoA lert Caspase-3分析试剂盒检测caspase-3的活性。结果DMTCC I具有抑制人白血病HL-60细胞增殖的作用,其IC50值为0.24μmol.L-1。流式细胞仪和DNA琼脂糖凝胶电泳结果显示,DMTCC I可诱导HL-60细胞凋亡。在经DMTCC I处理的HL-60细胞中,survivin和Bc l-xL蛋白的表达水平下调,Bad和Bax蛋白的表达水平上调,Bc l-2蛋白的表达水平无变化,caspase-9,caspase-3,caspase-6,PARP,DFF45和lamin B被分别裂解,产生相应裂解产物。在HL-60细胞中,caspase-3的活性在1μmol.L-1DMTCCI处理3 h时明显升高,在处理12 h时达到最高峰。结论DMTCCI可抑制人白血病HL-60细胞的增殖并诱导其发生细胞凋亡。Bcl-2家族蛋白、survivin和caspases家族蛋白可能参与了上述诱导HL-60细胞凋亡的过程。

关键词： DNA引物酶 DMTCCI 细胞凋亡 HL-60