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A converged ubiquitin-proteasome pathw.y for the degradation of TOC and TOM tail-anchored receptors

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Journal of Integrative Plant Biology 2024年第5期66卷 1007-1023页

作者： Meijing Yang Shuai Chen Shey-Li Lim Lang Yang Jia Yi Zhong Koon Chuen Chan Zhizhu Zhao kam-Bo wong Junqi w.ng Boon Leong Lim School of Biological Sciences University of Hong KongPokfulam 999077Hong KongChina Department of Biology Southern University of Science and TechnologyShenzhen 518055China School of Biomedical Engineering Shenzhen UniversityShenzhen 518060China School of Life Sciences The Chinese Universityof Hong KongShatin 999077HongKongChina State Key Laboratory of Agrobiotechnology The Chinese University of Hong KongShatin 999077Hong KongChina HKU Shenzhen Institute of Research and Innovation Shenzhen 518052China

In plants,thousands of nucleus-encoded proteins translated in the cytosol are sorted to chloroplasts and mitochondria by binding to specific receptors of the TOC(translocon on the outer chloroplast membrane)and the TOM(translocon on the outer mitochondrial membrane)complexes for import into those *** degradation pathw.ys for these receptors are ***,w. discovered a converged ubiquitin-proteasome pathw.y for the degradation of Arabidopsis thaliana TOC and TOM tail-anchored *** receptors are ubiquitinated by E3 ligase(s)and pulled from the outer membranes by the AAA+adenosine triphosphatase CDC48,after w.ich a previously uncharacterized cytosolic protein,transmembrane domain(TMD)-binding protein for tail-anchored outer membrane proteins(TTOP),binds to the exposed TMDs at the C termini of the receptors and CDC48,and delivers these complexes to the 26S proteasome.

关键词： 26S proteasome CDC48 SP1 TOC TOM ubiquitination

Computational Approaches for Traditional Chinese Painting: From the “Six Principles of Painting” Perspective

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Journal of Computer Science & Technology 2024年第2期39卷 269-285页

作者：张玮张建伟黄锦畦王懿芳封颖超杰王璐玮陈为 State Key Laboratory of CAD&CG Zhejiang UniversityHangzhou 310058China Department of Computer Science and Engineering The Hong Kong University of Science and Technology Hong Kong 999077China Kellogg School of Management Northwestern UniversityEvanston 60208U.S.A Laboratory of Art and Archaeology Image Zhejiang UniversityHangzhou 310058China

Traditional Chinese painting(TCP)is an invaluable cultural heritage resource and a unique visual art *** recent years,there has been a grow.ng emphasis on the digitalization of TCP for cultural preservation and *** resulting digital copies have enabled the advancement of computational methods for a structured and systematic understanding of *** explore this topic,w. conduct an in-depth analysis of 94 pieces of *** examine the current use of computer technologies on TCP from three perspectives,based on numerous conversations w.th ***,in light of the“Six Principles of Painting”theory,w. categorize the articles according to their research focus on artistic ***,w. create a four-stage framew.rk to illustrate the purposes of TCP ***,w. summarize the popular computational techniques applied to *** w.rk also provides insights into potential applications and prospects,w.th professional opinion.

关键词： traditional Chinese painting(TCP) digital humanities cultural heritage computer vision deep learning

Schematic interface of sound creation for computer animators

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2006年第7期7卷 1141-1151页

作者： TONG kam-pang Maya wong kam-w.h School of Creative Media City University of Hong Kong Hong Kong China

As an audiovisual medium, computer animations require superior image quality and professional soundtrack to lead audiences into their fascinating virtual w.rld. w.thout sound, the impact of storytelling is reduced or the story is even not under- standable. Despite the importance of sound, most animators are unfamiliar w.th sound editing softw.re. Limited budget projects such as independent or student w.rks have difficulty hiring sound professionals to create tailor-made soundtrack. Therefore, w. need a suitable sound tool to express their individual ideas. In this paper, w. propose an approach using schematic for both computer animation and sound. Our approach provides (1) physical simulation on sound through animation parameters and (2) a new.channel for animators to add aesthetic values in sound through their experience of using schematics in existing animation softw.re. w. demonstrate our idea through several examples, such as Doppler shift, obstacle effect, importance, energetic, and sputtering effect.

关键词： Computer animation Multimedia Digital media Schematics Physical simulation Aesthetic Sound effects Soundtrack

铜绿假单胞菌对肺血管内皮细胞功能的影响及其机制

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中国医学科学院学报 2024年第1期46卷 1-10页

作者：张雷王俊轶何翔吴敏李国平黄锦伟澳门科技大学中药质量研究国家重点实验室中国澳门999078 成都市第三人民医院呼吸健康研究所过敏与精准医学实验室成都610000 北达科他大学医学与健康科学院生物医学科学系美国北达科他州大福克斯58203 中国科学院大学温州研究院浙江温州325000

目的探究铜绿假单胞菌(PA)感染对肺血管内皮细胞功能的影响,并探讨其加重小鼠肺部炎症反应的可能机制。方法PAO1菌株感染人肺微血管内皮细胞HULEC-5a 2 h后,采用逆转录实时荧光定量PCR(RT-qPCR)检测自噬相关基因5(ATG5)、6-磷酸果糖-2-... 详细信息

目的探究铜绿假单胞菌(PA)感染对肺血管内皮细胞功能的影响,并探讨其加重小鼠肺部炎症反应的可能机制。方法PAO1菌株感染人肺微血管内皮细胞HULEC-5a 2 h后,采用逆转录实时荧光定量PCR(RT-qPCR)检测自噬相关基因5(ATG5)、6-磷酸果糖-2-激酶/果糖-2,6-二磷酸酶3(PFKFB3)和钙黏蛋白5(CDH5)的mRNA表达,免疫荧光检测ATG5、PFKFB3和血管内皮钙黏蛋白(VE-cadherin)的蛋白表达。采用小干扰RNA(siRNA)分别敲减ATG5、PFKFB3后,RT-qPCR检测ATG5、PFKFB3和CDH5的mRNA表达,免疫荧光检测PFKFB3和VE-cadherin的蛋白表达,并采用乳酸测定试剂盒检测细胞内乳酸的水平。采用PAO1菌株感染小鼠后,肺组织切片病理染色观察小鼠肺部炎症,激光共聚焦显微镜观察并分析荧光标记的内皮细胞PFKFB3和VE-cadherin的表达量。结果与对照组比较,PAO1感染HULEC-5a细胞后,PFKFB3 mRNA和蛋白表达均增加(P均<0.05),CDH5 mRNA表达减少(P=0.023),VE-cadherin蛋白表达减少(P<0.001),且连续性被破坏,乳酸含量升高(P=0.017)。与PAO1感染HULEC-5a细胞比较,PAO1感染敲减PFKFB3的HULEC-5a细胞后,CDH5 mRNA表达增加(P=0.043),VE-cadherin荧光连续性得到恢复,乳酸含量降低(P=0.047);PAO1感染敲减ATG5的HULEC-5a细胞后,PFKFB3 mRNA(P=0.013)和蛋白表达(P=0.003)均增加,CDH5 mRNA(P=0.020)和VE-cadherin蛋白表达(P=0.001)均减少,乳酸含量升高(P=0.015)。PAO1感染小鼠后,病理HE染色结果显示,肺泡内明显的红细胞渗漏,炎症细胞浸润,肺泡间隔增宽和部分血管内皮细胞脱落。免疫荧光染色结果显示,与正常小鼠比较,PAO1感染小鼠内皮细胞PFKFB3表达增加,VE-cadherin荧光减弱,且不连续。结论PA可能通过AGT5调控PFKFB3通路,破坏肺血管内皮细胞功能,进而加重小鼠肺部炎症反应。

关键词：铜绿假单胞菌肺血管内皮细胞 6-磷酸果糖-2-激酶/果糖-2,6-二磷酸酶3 自噬相关基因5 感染

Development of An ICR Mouse Bioassay for Toxicity Evaluation in Neurotoxic Poisoning Toxins-Contaminated Shellfish

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Biomedical and Environmental Sciences 2013年第5期26卷 346-364页

作者： wong Chun Kw.n HUNG Patricia kam Kai Man Biotoxin Laboratory Microbiology DivisionPublic Health Laboratory Services BranchCentre for Health ProtectionDepartment of HealthShek Kip MeiKowloon

Objective To develop an ICR （female） mouse bioassay （MBA） for toxicity confirmation and evaluation of neurotoxins （brevetoxins）-contaminated shellfish. Methods Brevetoxins （BTX-B） as a causative agent of neurotoxic shellfish poisoning （NSP） under different shellfish matrices w.re intraperitoneally injected at different doses into mice to study their toxic effects and to differentiate the range of lethal and sublethal dosages. Their sensitivity and specificity w.re analyzed w.th 2 competitive ELISA kits for quantitative determination of standard BTX-B and dihydroBTX-B under different shellfish matrix-diluent combinations. Detection rates of MBA and tw. antibody-based assays for BTX-B from field NSP-positive shellfish samples w.re compared. Results BTX-B could be detected in shellfish tissues at concentration of 50-400 μg/100 g under shellfish matrix-Tw.en-saline media, w.ich w.re appropriate to identify toxic shellfish at or above the regulatory limit （80 μg/100 g shellfish tissues）. The LD 50 identified w.s 455 g/kg for BTX-B under general shellfish matrices （excluding oyster matrices） dissolved in Tw.en-saline. The presence of shellfish matrices, of oyster matrices in particular, retarded the occurrence of death and toxicity presentation in mice. Tw. antibody-based assays, even in the presence of different shellfish matrix-diluent combinations, show.d acceptable results in quantifying BTX-B and dihydroBTX-B w.ll below.the regulatory limit. Conclusion The tw. ELISA analyses agree favorably （correlation coefficient, r 0.96; Student＇s t-tests, P〉0.05） w.th the developed bioassay.

关键词： Antibody-based assay Brevetoxin ICR female mice LD 50 Mouse bioassay Neurotoxic shellfish poisoning

Attenuation of mitochondrial,but not cytosolic,Ca2+ overload reduces myocardial injury induced by ischemia and reperfusion

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Acta Pharmacologica Sinica 2006年第7期27卷 911-918页

作者： Kenneth w. kam James SK SHAM Tlak-ming wong Department of Physiology the University of Hong KongHong Kong SARChina Division of Pulmonary Critical Care Medicine Johns Hopkins School of MedicineBaltimoreMaryland 21224USA

Aim:Attenuation of mitochondrial Ca2+([Ca2+]m),but not cytosolic Ca2+([Ca2+]c),overload improves contractile *** hypothesized that attenuation of[Ca2+]m,but not[Ca2+]c,overload confers cardioprotection against ischemia/reperfusion-induced ***:Infarct size from isolated perfused rat heart,cell viability,and electrically-induced Ca2+transient in isolated rat ventricularmyocytes w.re *** determined the effects of BAPTA-AM,a Ca2+chelator,at concentrations that abolish the overload of both[Ca2+]cand[Ca2+]m,and ruthenium red,an inhibitor of mitochondrial uniporter of Ca2+transport,atconcentrations that abolish the overload of[Ca2+]m,but not[Ca2+]c,on cardiacinjury induced by ischemia/***:Attenuation of both[Ca2+]mand[Ca2+]cby BAPTA-AM,and attenuation of[Ca2+]m,but not[Ca2+]c,overloadby ruthenium red,reduced the cardiac injury observations,indicating the impor-tance of[Ca2+]min cardioprotection and contractile recovery in response to is-chemia/***:The study has provided unequivocal evidenceusing a cause-effect approach that attenuation of[Ca2+]m,but not[Ca2+]c,over-load is responsible for cardioprotection against ischemia/*** also confirmed the previous observation that attenuation of[Ca2+]m,but not[Ca2+]c,by ruthenium red improves contractile recovery follow.ng ischemia/reperfusion.

关键词： ruthenium red BAPTA-AM infarct size electrically-induced Ca2+transient isolated perfused rat heart cardiac myocytes

Development of advanced nursing practice in China: Act local and think global

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International Journal of Nursing Sciences 2018年第2期5卷 101-104页

作者： Frances kam Yuet wong School of Nursing The Hong Kong Polytechnic UniversityHunghomKowloonHong Kong SARChina

This paper discusses the development of advanced nursing practice in China in the context of global *** scope of nursing is evolving over time,w.th increasing demands for the management of complex healthcare situations at individual,community and system *** demands are aggravated by the specialization of medical practice,w.th advanced treatment plans and patients requiring care in focused *** qualifications and competencies of the initial entrants into nursing practice are not adequate to deal w.th these *** nursing practice(ANP)developed first in response to service demands,and education programs w.re introduced to prepare nurses for practicing at a higher *** paper w.ll first review.the historical development of ANP in China,follow.d by a discussion of the differentiation of competence levels in nursing practice and the classification of *** concludes by exploring how.education in combination w.th experience protected by regulation of practice can support nurses to gradually evolve from registered nurse,specialty nurse to advanced practiced nurse.

关键词： nurse nursing education

A Survey on Recent Advances and Challenges in Reinforcement Learning Methods for Task-oriented Dialogue Policy Learning

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Machine Intelligence Research 2023年第3期20卷 318-334页

作者： w.i-Chung Kw.n Hong-Ru w.ng Hui-Min w.ng kam-Fai wong The Systems Engineering and Engineering Management Department The Chinese University of Hong KongHong Kong 999077China

Dialogue policy learning(DPL)is a key component in a task-oriented dialogue(TOD)*** goal is to decide the next action of the dialogue system,given the dialogue state at each turn based on a learned dialogue *** learning(RL)is w.dely used to optimize this dialogue *** the learning process,the user is regarded as the environment and the system as the *** this paper,w. present an overview.of the recent advances and challenges in dialogue policy from the perspective of *** specifically,w. identify the problems and summarize corresponding solutions for RL-based dialogue policy *** addition,w. provide a comprehensive survey of applying RL to DPL by categorizing recent methods into five basic elements in *** believe this survey can shed light on future research in DPL.

关键词： Dialogue policy learning(DPL) task-oriented dialogue system(TOD) reinforcement learning(RL) dialogue system Markov decision process

Patients w.th Helicobacter pylori positive and negative duodenal ulcers have distinct clinical characteristics

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w.rld Journal of Gastroenterology 2005年第23期11卷 3518-3522页

作者： Kent-Man Chu Ka-Fai Kw.k Simon Law.kam-Ho wong Department of Surgery

AIM: To assess the clinical characteristics of Helicobacterpylori(H pylori) negative duodenal ***: Patients w.th an endoscopic diagnosis of duodenal ulcer betw.en 1996 and 2002 w.re included in the present study. Patients w.re considered to be negative for Hpylori, if both histological examination and rapid urease test of biopsy specimens w.re negative. A comparison w.s made betw.en patients w.th H pyloripositive and negative duodenal ***: A total of 1 343 patients w.re studied. Their mean age w.s 54.7±0.5 years. There w.s a male preponderance (M:F = 2.5:1). Three hundred and ninetyeight patients (29.6%) did not have H pylori infection. The annual proportion of patients w.th H pylori negative duodenal ulcers increased progressively from 1996 to2002. On multivariate analysis, patients w.th H pylorinegative duodenal ulcer w.re more likely to be older, have concomitant medical problem, pre-existing malignancy, recent surgery, underlying sepsis, or taken non-steroidal anti-inflammatory drugs. In terms of clinical presentations, patients w.th H pylori negative duodenal ulcer w.re more likely to present w.th bleeding, multiple ulcers and larger ***: The proportion of patients w.th H pylori negative duodenal ulcers is on the rise because of a continued drop in incidence of H pylori positive duodenalulcers in recent years. Such patients have distinct clinical characteristics and it is important to ascertain the H pylori status before starting eradication therapy.

关键词： Helicobacter pylori Duodenal ulcer