Attenuation of mitochondrial,but not cytosolic,Ca2+ overload reduces myocardial injury induced by ischemia and reperfusion

作     者：Kenneth WL KAM James SK SHAM Tlak-ming WONG 

作者机构：Department of Physiologythe University of Hong KongHong Kong SARChina Division of Pulmonary Critical Care MedicineJohns Hopkins School of MedicineBaltimoreMaryland 21224USA 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2006年第27卷第7期

页      面：911-918页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Project supported by the Cardiovascular Physiology Research Fund donated by LCST(Holdings)Ltd WYY was supported by a Studentship from the University of Hong Kong 

主　　题：ruthenium red BAPTA-AM infarct size electrically-induced Ca2+transient isolated perfused rat heart cardiac myocytes 

摘      要：Aim:Attenuation of mitochondrial Ca2+([Ca2+]m),but not cytosolic Ca2+([Ca2+]c),overload improves contractile *** hypothesized that attenuation of[Ca2+]m,but not[Ca2+]c,overload confers cardioprotection against ischemia/reperfusion-induced ***:Infarct size from isolated perfused rat heart,cell viability,and electrically-induced Ca2+transient in isolated rat ventricularmyocytes were *** determined the effects of BAPTA-AM,a Ca2+chelator,at concentrations that abolish the overload of both[Ca2+]cand[Ca2+]m,and ruthenium red,an inhibitor of mitochondrial uniporter of Ca2+transport,atconcentrations that abolish the overload of[Ca2+]m,but not[Ca2+]c,on cardiacinjury induced by ischemia/***:Attenuation of both[Ca2+]mand[Ca2+]cby BAPTA-AM,and attenuation of[Ca2+]m,but not[Ca2+]c,overloadby ruthenium red,reduced the cardiac injury observations,indicating the impor-tance of[Ca2+]min cardioprotection and contractile recovery in response to is-chemia/***:The study has provided unequivocal evidenceusing a cause-effect approach that attenuation of[Ca2+]m,but not[Ca2+]c,over-load is responsible for cardioprotection against ischemia/*** also confirmed the previous observation that attenuation of[Ca2+]m,but not[Ca2+]c,by ruthenium red improves contractile recovery following ischemia/reperfusion.