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clinically relevant factors associated with quantitative optical coherence tomography angiography metrics in deep capillary plexus in patients with diabetes

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Eye and Vision 2020年第1期7卷 64-74页

作者： Fang Yao Tang Erica O.chan Zihan Sun Raymond Wong Jerry Lok Simon szeto Jason c.chan Alexander Lam clement c.Tham Danny S.Ng carol Y.cheung Department of Ophthalmology and Visual Sciences The Chinese University of Hong KongHong Kong Special Administrative RegionKowloonChina Hong kong Eye Hospital Hong Kong Special Administrative RegionKowloonChina cUHk Eye centre Hong Kong Eye Hospital147K Argyle StreetKlnKowloonHong KongChina

Background:To test clinically relevant factors associated with quantitative artifact-free deep capillary plexus(DcP)metrics in patients with diabetes mellitus(DM).Methods:563 eligible eyes(221 with no diabetic retinopathy[DR],135 with mild DR,130 with moderate DR,and 77 with severe DR)from 334 subjects underwent optical coherence tomography-angiography(OcT-A)with a swept-source OcT(Triton DRI-OcT,Topcon,Inc.,Tokyo,Japan).Strict criteria were applied to exclude from analysis those DcP images with artifacts and of poor quality,including projection artifacts,motion artifacts,blurriness,signal loss,B-scan segmentation error,or low-quality score.A customized MATLAB program was then used to quantify DcP morphology from the artifact-free DcP images by calculating three metrics:foveal avascular zone(FAZ),vessel density(VD),and fractal dimension(FD).Results:166(29.5%)eyes were excluded after quality control,leaving in the analysis 397 eyes(170 with no DR,101 with mild DR,90 with moderate DR,36 with severe DR)from 250 *** the multiple regression models,larger FAZ area was associated with more severe DR(β=0.687;p=0.037),shorter axial length(AL)(β=−0.171;p=0.003),thinner subfoveal choroid thickness(β=−0.122;p=0.031),and lower body mass index(BMI)(β=−0.090;p=0.047).Lower VD was associated with more severe DR(β=−0.842;p=0.001),shorter AL(β=0.107;p=0.039),and poorer visual acuity(VA)(β=−0.133;p=0.021).Lower FD was associated with more severe DR(β=−0.891;pconclusions:Quantitative artifact-free DcP metrics are associated with VA,DR severity,AL,subfoveal choroidal thickness,age,and BMI in diabetic *** effects of ocular and systemic factors should be considered for meaningful interpretations of DcP changes in DM patients.

关键词： Optical coherence tomography angiography Diabetic retinopathy Deep capillary plexus Visual acuity

Vesicle-bound and free microRNAs in spent cell culture medium and biological fluids

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Advances in Bioscience and Biotechnology 2012年第8期3卷 1091-1095页

作者： Gang Wang ka-Bik Lai Bonnie ching-Ha kwan kai-Ming chow Philip kam-Tao Li cheuk-chun szeto Department of Medicine & Therapeutics The Chinese University of Hong Kong Hong Kong China

Background: In addition to the control of gene transla- tion intra-cellularly, microRNAs (miRNAs) have been found to exist extracellularly. However, extracellular miRNA identified in previous studies were largely confined to microvesicles. It remains uncertain whether free extracellular miRNA exists. Methods: We quantify a panel of miRNAs (miRNA 200 family, miR-205, and miR-192) in spent culture medium of renal tubular epithelial cells, as well as serum and urine from patients with systemic lupus erythematosus and healthy controls. Microvesicle bound and free miRNA were separated by ultracentrifugation. Results: In spent cell culture medium, we found substantial amount of miR-200a, miR-200b, miR-200c, miR-141, miR-429, miR-205, and miR-192 in microvesicles, as well as the presence of miR-200a, miR-200b, miR-429, miR-205 and miR-192, but not miR-200c or miR-141 as non-vesicle bound free form. In healthy individuals, we found substantial amount of miR-200a, miR-200b, miR-200c, miR-141 and miR-192 in microvesicles from serum and urine, while only miR-141 exists as free form in both serum and urine. There was no significant difference in serum or urinary free miR-141 levels between SLE patients and healthy controls. In SLE patients, urinary free miR-141 level was signifiantly higher than serum. Neither serum nor urinary free miR-141 levels correlated with lupus disease activity. conclusion: We found miRNA is present extracellularly, both within microvesicles and as free form, in spent cell culture medium, serum and urine. The biological role of extracellular miRNA requires further study.

关键词： cell Signaling Fibrosis cytokine

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母乳成分在-80℃保存过程中的稳定性研究

食品研究与开发 2017年第4期38卷 5-9页

作者：方芳李婷司徒文佑云战友内蒙古伊利实业集团股份有限公司内蒙古呼和浩特010110

通过对母乳脂肪酸、维生素、活性成分以及p H值和酸度在超低温冷冻条件下的稳定性研究,最终制定伊利母乳研究实物库的母乳质量标准,以确保伊利母乳研究实物库中的母乳样本在-80℃保存过程中营养成分的损失率最小。试验结果表明,母乳在-8... 详细信息

通过对母乳脂肪酸、维生素、活性成分以及p H值和酸度在超低温冷冻条件下的稳定性研究,最终制定伊利母乳研究实物库的母乳质量标准,以确保伊利母乳研究实物库中的母乳样本在-80℃保存过程中营养成分的损失率最小。试验结果表明,母乳在-80℃冷冻保存12个月的过程中,随着保存时间的延长,维生素含量显著降低,脂肪酸、核苷酸含量以及p H值和酸度没有发生显著性变化。母乳-80℃保存3个月时,V_B_2和V_A的含量分别减少为新鲜母乳的35%和29%,V_c含量无法检出;-80℃保存12个月时,烟酸含量减少为新鲜母乳的69%。因此,从现有检测指标来看,母乳库的质量标准应根据分析研究的母乳成分而定,若分析稳定性较差的营养成分如维生素尽量使用新鲜母乳。

关键词：母乳成分稳定性

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乳酸菌对酸奶保鲜作用的研究

食品安全质量检测学报 2017年第2期8卷 601-608页

作者：孙婷张兴昌司徒文佑陈世贤内蒙古伊利实业集团股份有限公司伊利创新中心呼和浩特010110 内蒙古乳业技术研究院有限责任公司呼和浩特010110

目的比较4种具有保鲜作用的乳酸菌对酸奶中霉菌的抑制效果及在发酵和贮藏过程中对酸奶品质的影响。方法将4种乳酸菌添加到发酵乳中,比较对酸乳中霉菌的抑制作用,并对酸奶酸度、活菌数、质构和感官品质进行检测。结果按厂家推荐量和相同... 详细信息

目的比较4种具有保鲜作用的乳酸菌对酸奶中霉菌的抑制效果及在发酵和贮藏过程中对酸奶品质的影响。方法将4种乳酸菌添加到发酵乳中,比较对酸乳中霉菌的抑制作用,并对酸奶酸度、活菌数、质构和感官品质进行检测。结果按厂家推荐量和相同活菌数(2.0×10~6 cFU/m L)添加4种乳酸菌均对霉菌的生长具有抑制作用,其中,乳酸菌A和c的抑菌效果较好;按照厂家推荐量添加4种乳酸菌发现,乳酸菌A和B的添加对酸奶的发酵时间、贮藏期内的酸度、活菌数、质构和感官均无影响,而乳酸菌c和D的添加会对酸奶贮藏期内的酸度和感官产生影响。结论乳酸菌A能够有效抑制酸奶中霉菌滋生,同时不会对酸奶的品质产生影响。

关键词：乳酸菌酸奶保鲜霉菌

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中国城市13~36月龄幼儿挑食行为与自报患病的关联分析

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中国食物与营养 2022年第9期28卷 75-81页

作者：李静文赵艾蓝航莲张健任中夏李玭司徒文佑王培玉张玉梅北京大学公共卫生学院营养与食品卫生学系北京100191 清华大学万科公共卫生与健康学院北京100084 内蒙古伊利实业集团股份有限公司呼和浩特010020 北京大学公共卫生学院社会医学与健康教育系北京100191

目的:描述中国城市13~36月龄幼儿挑食行为现状,探讨挑食行为与自报患病情况的关系。方法:于2019-2020年对中国10城市924名13~36月龄幼儿开展横断面的营养与健康调查,问卷法收集主要喂养人自报幼儿的挑食与否、挑食食物种类及近3个月患... 详细信息

目的:描述中国城市13~36月龄幼儿挑食行为现状,探讨挑食行为与自报患病情况的关系。方法:于2019-2020年对中国10城市924名13~36月龄幼儿开展横断面的营养与健康调查,问卷法收集主要喂养人自报幼儿的挑食与否、挑食食物种类及近3个月患病情况,使用χ~2检验和Logistic回归法分析挑食行为自报疾病的关系。结果:被调查城市幼儿总体挑食率为36.0%,幼儿居住地为北京市、24~36月龄、首胎出生、父亲文化程度在大专及以上者挑食率较低(Pc、维生素E、铁元素、硒元素、铜元素、锰元素摄入水平低于非挑食者(PcI:1.374~2.980)。挑食豆类(OR=2.294,95%cI:1.072~4.911)、蔬菜(OR=2.288,95%cI:1.401~3.735)、水果(OR=3.643,95%cI:1.481~8.959)、蛋类(OR=2.838,95%cI:1.095~7.356)与便秘相关。挑食谷类(OR=2.451,95%cI:1.003~5.992)和水果(OR=3.007,95%cI:1.203~7.521)与过敏性疾病的发生有关。结论:中国城市13~36月龄幼儿挑食率较高,挑食行为与幼儿自报便秘、过敏性疾病等存在一定关联。

关键词：幼儿挑食患病

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