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Genetics,resistance mechanism,and breeding of powdery mildew resistance in cucumbers(Cucumis sativus L.)

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Horticultural Plant Journal 2023年第4期9卷 603-615页

作者： Jingtao Nie Qi Yuan Wanlu Zhang junsong pan College of Horticulture Science Zhejiang Agriculture&Forestry UniversityHangzhouZhejiang 311300China Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable Ministry of Agriculture and Rural AffairsHangzhouZhejiang 311300China Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province College of Horticulture ScienceZhejiang A&F UniversityHangzhouZhejiang 311300China School of Agriculture and Biology Shanghai Jiao Tong UniversityShanghai 200241China

Cucumber is an important vegetable worldwide,and powdery mildew(PM)is a common and serious disease of *** disease-resistant cucumber varieties is the most advantageous strategy to control this *** recent years,exploration and identification of cucumber PM resistance genes have achieved great advancement,and many genes have been cloned and verified using different ***,the resistance mechanism of cucumber PM is still unclear,and many ambiguities need to be elucidated *** this review,we summarized the research advances in PM resistance in cucumbers,including genetic analysis,quantitative trait locus mapping,map-based cloning,transcriptomics,mlo-mediated PM resistance,and mining of noncoding RNAs involved in ***,the research directions and the problems that need to be solved in the future were discussed.

关键词： Cucumber Powdery mildew resistance Gene mapping Resistance mechanism Molecular breeding Mildew resistance locus O(MLO)

Asynchronous meiosis in Cucumis hystrix–cucumber synthetic tetraploids resulting in low male fertility

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The Crop Journal 2016年第4期4卷 275-279页

作者： Yonghua Han junsong pan Paradee Thammapichai Zongyun Li Yiqun Weng Institute of Integrative Plant Biology School of Life Sciences Jiangsu Normal University Department of Horticulture University of Wisconsin School of Agriculture and Biology Shanghai Jiao Tong University United States Department of Agriculture/Agricultural Research Service Vegetable Crops Research Unit

Interspecific hybridization and allopolyploidization contribute to the improvement of many important crops. Recently, we successfully developed an amphidiploid from an interspecific cross between cucumber(Cucumis sativus, 2n = 2x = 14) and its relative C. hystrix(2n = 2x = 24) followed by chemical induction of chromosome doubling. The resulting allotetraploid plant was self-pollinated for three generations. The fertility and seed set of the amphidiploid plants were very low. In this study, we investigated the meiotic chromosome behavior in pollen mother cells with the aid of fluorescence in situ hybridization, aiming to identify the reasons for the low fertility and seed set in the amphidiploid plants. Homologous chromosome pairing appeared normal, but chromosome laggards were common, owing primarily to asynchronous meiosis of chromosomes from the two donor genomes. We suggest that asynchronous meiotic rhythm between the two parental genomes is the main reason for the low fertility and low seed set of the C. hystrix–cucumber amphidiploid plants.

关键词： Cucumber Cucumis hystrix Amphidiploid Meiosis Asynchrony

Quantitative trait locus analysis of lateral branch-related traits in cucumber(Cucumis sativus L.)using recombinant inbred lines

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Science China(Life Sciences) 2008年第9期51卷 833-841页

作者： JIANG Su YUAN XiaoJun pan junsong HE HuanLe CAI Run School of Agriculture and Biology Shanghai Jiaotong UniversityShanghai 200240China

A group of 224 recombinant inbred lines (RILs) was derived from a narrow cross between 2 cucumber (Cucumis sativus L.) lines, namely, S94 (Northern China type with weak lateral branch growth potential and early lateral branch sprouting time) and S06 (Northern European type with strong lateral branch growth potential and late lateral branch sprouting time). These lines were then used for investigating lateral branch-related traits. A total of 36 quantitative trait loci (QTLs) were detected for the following 4 lateral branch-related traits: lateral branch average length (LBAL), lateral branch total length (LBTL), lateral branch number (LBN), and first lateral branch node (FLBN). Further, each QTL explained 3.1% (lbtl2.1, spring) to 32.3% (lbn2.3, spring) of the observed phenotypic variance. Eleven QTLs (lbal1.1, lbtl1.1, lbn1.2, flbn1.2, etc.) for different traits were found to be clustered on the e23m18d-ME23EM6c section (7.4 cM) of linkage group (LG) 1; further, 15 QTLs (lbal2.1, lbtl2.1, lbn2.1, flbn2.1, etc.) were found to be clustered on the S94A1-ME4SA4a section (13.9 cM) of LG2. Twenty-one QTLs explained more than 10% of the phenotypic variance. Moreover, lbtl1.3 (autumn, 26.2%, logarithm of odds (LOD) = 17.4; spring, 26.9%, LOD = 17.9) had stable position and contribution in both seasons. Several se-quence-anchor markers (CMBR40, F, CS30, S94A1, CSWTA11B, etc.) were closely linked with some QTLs for LBAL, LBTL, LBN, and FLBN, which can be used for the marker-assisted selection to improve the plant architecture in cucumber breeding.

关键词： Cucumis sativus L. lateral branching recombinant inbred lines quantitative trait loci

Reporter-based screen for Arabidopsis mutants compromised in nonhost resistance

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Chinese Science Bulletin 2008年第7期53卷 1027-1034页

作者： CHEN HuaMin pan junsong ZHAO XiuXiang ZHOU JianMin CAI Run School of Agriculture and Biology Shanghai Jiaotong University Shanghai 200240 China National Institute of Biological Sciences Beijing 102206 China College of Plant Protection Shenyang Agricultural University Shenyang 110161 China

Plants are exposed to many potentially pathogenic microbes in the environment, but each species is only susceptible to a limited number of pathogens. The broad resistance is referred to as nonhost re-sistance. To date, little is known about the underlying mechanism of nonhost resistance and the sig-naling transduction process. Here we describe a simple method for isolating Arabidopsis nonhost re-sistance mutants against a nonadapted bacterial pathogen. A RAP2.6 promoter-driven LUC reporter system was developed to replace the tedious bacterial growth assay during the primary screening. The RAP2.6-LUC reporter gene is normally induced by the virulent bacterium Pseudomonas syringae pv tomato but not the nonadapted bacterium P. syringae pv phaseolicola. By using this method we iso-lated 4 mutants displaying strong reporter activity in response to P. syringae pv phaseolicola, which were characterized in some details. ebs1, ebs2, ebs3, and ebs4 (enhanced bacterial susceptibility) were compromised in resistance against P. syringae pv phaseolicola and/or P. syringae pv tomato. In addi-tion, ebs4 showed enhanced hypersensitive response to the incompatible bacterium P. syringae pv tomato (avrB). These results demonstrated that the method is suited for large scale screening for nonhost resistance mutants.

关键词：突变体抗寄生植物电阻基因

Construction of a cucumber genetic linkage map with SRAP markers and location of the genes for lateral branch traits

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Science China(Life Sciences) 2005年第3期48卷 213-220页

作者： WANG Gang pan junsong LI Xiaozun HE Huanle WU Aizhong CAI Run Agriculture and Biology School of Shanghai Jiaotong University Shanghai201101China

Using SRAP(sequence-related amplified polymorphism)markers a genetic linkage map of cucumber was constructed with a population consisting of 138 F_(2) individuals derived from a cross of the two cucumber lines,S06 and *** the survey of parental polymorphisms with 182 primer combinations,64 polymorphism-revealing primer pairs were screened out,which generated totally 108 polymorphic bands with an average of 1.7 bands per primer pair and at most 6 bands from one primer *** constructed molecular linkage map included 92 loci,distributed in seven linkage groups and spanning 1164.2 cM in length with an average genetic distance of 12.6 cM between two neighboring *** on this linkage map,the quantitative trait loci(QTL)for the lateral branch number(lbn)and the lateral branch average length(lbl)in cucumber were identified by QTLMapper1.6.A major QTL lbn1 located between ME11SA4B and ME5EM5 in LG2 could explain 10.63%of the total variation with its positively effecting allele from S06.A major QTL lbl1 located between DC1OD3 and DC1EM14 in LG2 could account for 10.38%of the total variation with its positively effecting allele from S_(06).

关键词： cucumber SRAP(sequence-related amplified polymorphism) molecular linkage map lateral branch gene quan-titative trait loci(QTL).

Efficient Transposition of the Retrotransposon Tnt1 in Cucumber(Cucumis sativus L.)

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Horticultural Plant Journal 2018年第3期4卷 111-116页

作者： ZHANG Qi DU Hui LV Duo XIAO Tingting pan Jian HE Huanle WANG Gang CAI Run WENG Yiqun pan junsong Plant Science Department School of Agriculture and BiologyShanghai Jiao Tong University USDA-ARS Vegetable crops Research UnitHorticulture DepartmentUniversity of Wisconsin

Tnt1 is an active retrotransposon originally identified in tobacco(Nicotiana tabacum L.)(Grandbastien et al.,1989),but its transposition activity could be activated through tissue culture in other plant *** insertions are stable and inheritable in the progeny,which has made it a valuable and versatile tool for developing insertional mutagenesis libraries in several plant ***,we explored its utility for mutagenesis in cucumber(Cucumis sativus L.).T_3 Tnt1 transgenic cucumber plants were subjected to tissue culture to regenerate self-pollinated *** PCR and analyses and Southern hybridization,we found regenerated plants maintained the original Tnt1 insertion and created new insertions suggesting characteristic re-transposition activity of Tnt1 during this *** genome walking,some flanking sequences of Tnt1 insertions were recovered in regenerated *** results demonstrated that Tnt1 could be stably inherited and re-transposable during tissue culture in cucumber and that it is feasible to use for developing an insertional mutagenesis library for cucumber.

关键词： cucumber Tnt1 retrotransposon tissue culture mutagenesis library

Comparative Transcriptome Analyses Reveal Genes Related to Spine Development in Cucumber (Cucumis sativus)

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Phyton-International Journal of Experimental Botany 2023年第4期92卷 1059-1070页

作者： Yue Chen Huan Wang Taibai Xu Peng Zhou junsong pan Weiwei She Weiwei Zhang Department of Plant Science and Technology Shanghai Vocational College of Agriculture and ForestryShanghai201699China School of Agriculture and Biology Shanghai Jiaotong UniversityShanghai200240China

Fruit spine is an important quality trait of *** better understand the molecular basis of cucumber spine development and function,RNA-Seq was performed to identify differentially expressed genes(DEGs)in fruit spines of different development stages,namely,8 days before anthesis(SpBA8),anthesis(SpA)and 8 days after anthesis(SpAA8).Stage-wise comparisons obtained 2,259(SpBA8 ***),4,551(SpA ***8),and 5,290(SpBA8 ***8)*** the DEGs were classified into eight expression clusters by trend *** these DEGs,in addition to the Mict,Tril,CsTTG1,CsMYB6,NS,and Tu genes that have been reported to regulate fruit spine formation,we found that the CsHDG11,CsSCL8,CsSPL8,CsZFP6 and CsZFP8 may also be involved in spine development in *** study provides a theoretical basis for further research on molecular mechanisms of spine development in cucumber.

关键词： Cucumber transcriptome spine development differential expression genes

Photoexcited CRYPTOCHROME 1 Interacts Directly with G-Protein 13 Subunit AGB1 to Regulate the DNA-Binding Activity of HY5 and Photomorphogenesis in Arabidopsis

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Molecular Plant 2018年第10期11卷 1248-1263页

作者： Hongli Lian Pengbo Xu Shengbo He Jun Wu Jian pan Wenxiu Wang Feng Xu Sheng Wang junsong pan Jirong Huang Hong-Quan Yang School of Agriculture and Biology Shanghai Jiao Tong University Shanghai 200240 China School of Life Sciences and Biotechnology Shanghai Jiao Tong University Shanghai 200240 China College of Life and Environmental Sciences Shanghai Normal University Shanghai 200234 China State Key Laboratory of Genetic Engineering Collaborative Innovation Center for Genetics and Development School of Life Sciences Fudan University Shanghai200438 China These authors contributed equally to this article.

Light and the heterotrimeric G-protein are known to antagonistically regulate photomorphogenesis in Arabidopsis. However, whether light and G-protein coordinate the regulation of photomorphogenesis is largely unknown. Here we show that the blue light photoreceptor cryptochrome 1 （CRY1） physically inter-acts with the G-protein β subunit, AGB1, in a blue light-dependent manner. We also show that AGB1 directly interacts with HY5, a basic leucine zipper transcriptional factor that acts as a critical positive regulator of photomorphogenesis, to inhibit its DNA-binding activity. Genetic studies suggest that CRY1 acts partially through AGB1, and AGB1 acts partially through HY5 to regulate photomorphogenesis. Moreover, we demonstrate that blue light-triggered interaction of CRY1 with AGB1 promotes the dissociation of HY5 from AGB1. Our results suggest that the CRY1 signaling mechanism involves positive regulation of the DNA-binding activity of HY5 mediated by the CRY1-AGB1 interaction, which inhibits the association of AGB1 with HY5. We propose that the antagonistic regulation of HY5 DNA-binding activity by CRY1 and AGB1 may allow plants to balance light and G-protein signaling and optimize photomorphogenesis.

关键词： Arabidopsis cryptochrome heterotrimeric G-protein β subunit AGB1 HY5 photomorphogenesis

Construction of a genetic map with SRAP markers and localization of the gene responsible for the first-flower-node trait in cucumber (Cucumis sativus L.)

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Progress in Natural Science:Materials International 2005年第5期15卷 407-413页

作者： pan junsong*, WANG Gang*, LI Xiaozun, HE Huanle, WU Aizhong and CAI Run**(Agriculture and Biology School, Shanghai Jiaotong University, Shanghai 201101, China) Agric. and Biology Sch. Shanghai Jiaotong Univ. Shanghai 201101 China

With an F 2 population from the cross of two cucumber inbred lines, S06 and S52, sequence-related amplified polymorphism (SRAP) was used to construct a genetic linkage map in cucumber (Cucumis sativus L.). Sixty-four SRAP primer combinations generated 108 polymorphic bands in the F 2 population analysis. The average of polymorphic bands produced by one primer pair was 1.5, and the maximum was 5. Using Mapmaker 3.0, a linkage map was constructed, which consisted of 77 SRAP markers distributed in nine linkage groups (LOD≥3.0) and spanned 1114.2 cM with an average interval of 14.5 cM between markers. The gene for the first-flower-node trait, termed ffn, was mapped to linkage group Ⅸ, flanked by DC1EM5 and ME7EM2A at 10.3 cM and 12.1 cM distance, respectively.

关键词： cucumber (Cucumis sativus L.) SRAP (sequence-related amplified polymorphism) linkage map first flower node.