Efficient Transposition of the Retrotransposon Tnt1 in Cucumber(Cucumis sativus L.)

作     者：ZHANG Qi DU Hui LV Duo XIAO Tingting PAN Jian HE Huanle WANG Gang CAI Run WENG Yiqun PAN Junsong 

作者机构：Plant Science DepartmentSchool of Agriculture and BiologyShanghai Jiao Tong University USDA-ARSVegetable crops Research UnitHorticulture DepartmentUniversity of Wisconsin 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2018年第4卷第3期

页      面：111-116页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0902[农学-园艺学] 090102[农学-作物遗传育种] 090202[农学-蔬菜学] 

基　　金：supported by the National Natural Science Foundation of China(No.31471156) the Agri-X Project of Shanghai Jiao Tong University(Agri-X2015002) the Shanghai Graduate Education and Innovation Program(Horticulture) 

主　　题：cucumber Tnt1 retrotransposon tissue culture mutagenesis library 

摘      要：Tnt1 is an active retrotransposon originally identified in tobacco(Nicotiana tabacum L.)(Grandbastien et al.,1989),but its transposition activity could be activated through tissue culture in other plant *** insertions are stable and inheritable in the progeny,which has made it a valuable and versatile tool for developing insertional mutagenesis libraries in several plant ***,we explored its utility for mutagenesis in cucumber(Cucumis sativus L.).T_3 Tnt1 transgenic cucumber plants were subjected to tissue culture to regenerate self-pollinated *** PCR and analyses and Southern hybridization,we found regenerated plants maintained the original Tnt1 insertion and created new insertions suggesting characteristic re-transposition activity of Tnt1 during this *** genome walking,some flanking sequences of Tnt1 insertions were recovered in regenerated *** results demonstrated that Tnt1 could be stably inherited and re-transposable during tissue culture in cucumber and that it is feasible to use for developing an insertional mutagenesis library for cucumber.