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M1巨噬细胞回输治疗小鼠肝癌的研究

空军军医大学学报 2024年第7期45卷 749-755页

作者：卢羿同杨嘉蒙魏调霞赵俊龙秦鸿雁空军军医大学基础医学院医学遗传与发育生物学教研室陕西西安710032

目的研究回输M1型骨髓来源的巨噬细胞(BMDMs)对原位肝肿瘤小鼠模型的治疗作用。方法①分离野生小鼠的骨髓细胞并体外诱导成BMDMs(即M0型巨噬细胞),在IFN-γ+LPS诱导下极化为M1型巨噬细胞。通过肝内接种Luciferase-Hepa1-6细胞建立原位... 详细信息

目的研究回输M1型骨髓来源的巨噬细胞(BMDMs)对原位肝肿瘤小鼠模型的治疗作用。方法①分离野生小鼠的骨髓细胞并体外诱导成BMDMs(即M0型巨噬细胞),在IFN-γ+LPS诱导下极化为M1型巨噬细胞。通过肝内接种Luciferase-Hepa1-6细胞建立原位小鼠肝癌模型。在接种肿瘤7 d后,经尾静脉回输PBS(对照组)、M0型和M1型巨噬细胞,通过小动物活体成像技术检测小鼠肝脏肿瘤的生长状态。接种21 d后,分离并观察肝脏肿瘤大小及称取质量,确定回输不同类型巨噬细胞对小鼠肝脏肿瘤进展的影响。利用流式细胞仪分析肝脏肿瘤癌旁组织内巨噬细胞亚群变化。②分离GFP转基因小鼠的骨髓细胞,诱导分化成M0型和M1型巨噬细胞后经尾静脉回输至肝癌小鼠体内,利用流式细胞仪及免疫荧光等方法检测回输1、3、7 d后肝脏内GFP阳性的BMDMs细胞数。结果与对照组相比,回输M0型和M1型BMDMs均促进了肝肿瘤的生长(P<0.05)。回输M0型或M1型BMDMs后,癌旁组织中枯否细胞增多,骨髓单核来源的炎性巨噬细胞增多(P<0.05)。经尾静脉回输1×10^(6)个M0型或M1型GFP+BMDMs,1 d后约3×10^(3)个细胞驻留于肝脏中,并随着时间增长逐渐减少。结论回输M1型巨噬细胞并未能达到治疗肝肿瘤的目的,反而促进肝肿瘤的生长。

关键词：肝癌巨噬细胞细胞治疗肿瘤相关巨噬细胞

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南梁油田华64区沉积微相分析技术研究

石油学报 2008年第2期29卷 274-276,283页

作者：赵军龙西安石油大学油气资源学院陕西西安710065

根据油田资料,利用多种沉积相识别标志,确定了华64区沉积相划分方案。依照沉积微相研究内容和要求,确定了合理的技术路线,应用小波分析技术、灰色关联分析技术、交会图技术和测井相分析等方法,研究了沉积微相模式。该研究成果为油田今... 详细信息

根据油田资料,利用多种沉积相识别标志,确定了华64区沉积相划分方案。依照沉积微相研究内容和要求,确定了合理的技术路线,应用小波分析技术、灰色关联分析技术、交会图技术和测井相分析等方法,研究了沉积微相模式。该研究成果为油田今后开发调整方案的制定提出了依据。

关键词：沉积微相识别技术小波分析灰色关联分析取心井沉积微相模式测井相分析

Myeloid-specific targeting of Notch ameliorates murine renal fibrosis via reduced infiltration and activation of bone marrowderived macrophage

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Protein & Cell 2019年第3期10卷 196-210页

作者： Yali Jiang Yuanyuan Wang Pengfei Ma Dongjie An junlong zhao Shiqian Liang Yuchen Ye Yingying Lu Peng Zhang Xiaowei Liu Hua Han Hongyan Qin State Key Laboratory of Cancer Biology Department of Medical Genetics and Developmental BiologyFourth Military Medical UniversityXi'an 710032China Department of Nephrology Xijing HospitalFourth Military Medical UniversityChang-Le Xi Street#15Xi'an 710032China

Macrophages play critical roles in renal ***,macrophages exhibit ontogenic and functional heterogeneities,and which population of macrophages contributes to renal fibrosis and the underlying mechanisms remain *** this study,we genetically targeted Notch signaling by disrupting the transcription factor recombination signal binding protein-JK(RBP-J),to reveal its role in regulation of macrophages during the unilateral ureteral obstruction(UUO)-induced murine renal ***-specific disruption of RBP-J attenuated renal fibrosis with reduced extracellular matrix deposition and myofibroblast activation,as well as attenuated epithelial-mesenchymal transition,likely owing to the reduced expression of TGF-β.Mean while,RBP-J deletion significantly hampered macrophage infiltration and activation in fibrotic kidney,although their proliferation appeared *** using macrophage clearance experiment,we found that kidney resident macrophages made negligible contribution,but bone marrow(BM)-derived macrophages played a major role in renal *** mechanistic analyses showed that Notch blockade reduced monocyte emigration from BM by down-regulating CCR2 ***,we found that myeloid-specific Notch activation aggravated renal fibrosis,which was mediated by CCR2^+macrophages *** summary,our data have unveiled that myeloid-specific targeting of Notch could ameliorate renal fibrosis by regulating BM-derived macrophages recruitment and activation,providing a novel strategy for intervention of this disease.

关键词： renal fibrosis Notch signaling,macrophages heterogeneity EMT

Iron(Ⅲ) phthalocyanine chloride-catalyzed oxidation–aromatization of α,β-unsaturated ketones with hydrazine hydrate: Synthesis of 3,5-disubstituted 1H-pyrazoles

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Chinese Journal of Catalysis 2016年第4期37卷 571-578页

作者：赵军龙邱骏苟小锋花成文陈邦西北大学化学与材料科学学院，合成与天然功能分子化学教育部重点实验室陕西西安710069

We have developed an iron（III） phthalocyanine chloride‐catalyzed oxidation–aromatization ofα,β‐unsaturated ketones with hydrazine hydrate. Various 3,5‐disubstituted 1H‐pyrazoles were obtained in good to excellent yields. This method offers several advantages, including room‐tem‐perature conditions, short reaction time, high yields, simple work‐up procedure, and use of air as an oxidant. The catalyst can be recovered and reused five times without loss of activity.

关键词： Iron(III) phthalocyanine chloride Aromatization Pyrazole Michael addition Recyclable catalyst Green chemistry

“新医科”背景下的医学遗传学课程教学改革与探索

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中国优生与遗传杂志 2024年第3期32卷 644-648页

作者：胡昳旸丰帆梁世倩赵俊龙王涛王亮曹秀丽秦鸿雁中国人民解放军空军军医大学基础医学院医学遗传与发育生物学教研室陕西西安710032 中国人民解放军空军军医大学西京医院消化外科陕西西安710032

以人工智能、基因编辑为代表的科学技术飞速发展,带来医疗方式和医疗行为的巨大变革。“新医科”是适应当前科技产业革命和教学模式发展下的对医学教育体系和理念的重塑。在其引领下,针对医学遗传学授课内容陈旧、教学理念滞后、考核方... 详细信息

以人工智能、基因编辑为代表的科学技术飞速发展,带来医疗方式和医疗行为的巨大变革。“新医科”是适应当前科技产业革命和教学模式发展下的对医学教育体系和理念的重塑。在其引领下,针对医学遗传学授课内容陈旧、教学理念滞后、考核方式单一等问题,不断进行教学改革与探索,提出了针对性的解决方案。基于学科交叉的“医学遗传学+X”教学内容重构,培养综合运用知识解决复杂问题的能力;加强网络平台建设拓展学科深度和广度,提升科研技能和思维;通过教学形式的多样化,考核方式的多维度,激发学生学习热情,注重专业能力和素质培养。一系列的教学改革与探索,旨在提升教学质量,培养适应于现代信息科技条件下的能够为国家需求服务的创新型医学人才。

关键词：新医科医学遗传学教学改革

CuPc@C catalyzed decomposition of tosylhydrazones as a highly selective synthesis of N-alkyl substituted hydrazones

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CuPc@C catalyzed decomposition of tosylhydrazones as a highl...

中国化学会第四届卟啉与酞菁学术研讨会

作者： Jiaodan Wang Huiui duan junlong zhao Xiaofeng Gou Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education College of Chemistry ＆ Materials ScienceNorthwest University College of Science Xi’an University of Architecture and Technology

Metallophthalocyanines have many interesting properties and applications in several important technological fields,such as conductivity,chemical catalysis,photoelectric effect and nonlinear optical *** are very well known to catalyze a variety of organic reactions,so investigation into the redox properties of MPcs continue to be active areas of ***,due to their strong tendency towards aggregation and difficulty in separation from reaction mixture,immobilization of MPcs on activated carbon supports is carried out to make catalysts recoverable and *** group is interested in one-pot synthesis of heterocyclws via metallophthalocyanines-catalyzed addition/cyclization reactions,which provide economic,convenient and efficient approaches to potentially useful heterocyclic *** we report a highly selective one pot synthesis of functionalized N-alkyl substituted hydrazones by a simple condensation reaction of decomposing two equivalents of tosylhydrazones in the presence of a catalytic amount of CuPc@C was carried out and resulted in high product *** procedure proves to be advantageous in terms of low cost,high efficiency,high yield,simple operation,and wide application range,with a recyclable and therefore environmentally beneficial catalyst species.

关键词： Tosylhydrazones N-alkylation CuPc@C

MicroRNA-210 negative regulates Notch signaling mediatedinflammatory response in murine bone marrow derived macrophage

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MicroRNA-210 negative regulates Notch signaling mediatedinfl...

中国生物化学与分子生物学会第十一次会员代表大会暨2014年全国学术会议

作者： Fei Huang junlong zhao Hongyan Qin Hua Han Department of Medical Genetics and Developmental Biology Fourth Military Medical University

macrophage polarizationMacrophages participate in innate immune responses through different activation patterns,resulting in inflammatory reactions, tissue repairs, and other different biological *** preliminary studies using tumor models, we found that activating Notch signalingpromotes M1 polarization, while blocking Notch signaling promotes M2 polarization, suggestingthat Notch signaling is an important factor in the regulation of macrophage ***(miRNAs) are highly conversed, small non-coding RNA molecules thatplay an essential role in the regulation of gene expression and functions. By microarrayanalysis between the macrophages obtained from RBP-J, the key transcription factor ofNotch signaling, knockout mice and normal mice, we found that there were a set of differentiallyexpressed miRNAs, which suggested that the regulation of macrophage activationby Notch signaling may work through miRNA. In our present study, we found that miR-210, a Notch positive-regulated miRNA in murine bone marrow derived macrophage underinflammatory stimuli, worked as a negative regulator in inflammatory response mediatedby Notch *** real-time quantitative PCR, we found that miR-210 increased in M1 polarization inducedby LPS and IFN-r, but significantly downregulated in Lyz2-Cre-Rbpj-/- *** miR-210 mimic transfection, LPS/IFN-r-induced expression of proinflammatory cytokines,such as TNF-a, IL-6 and iNOS, were significantly reduced, which were significantlyincreased after miR-210 inhibitor transfection. Meanwhile, Bacterial phagocytic and killingexperiments showed that after miR-210 mimic transfection, the ability of macrophae uponphagocytic and bacterial killing were significantly decreased. Mixed lymphocyte reactionshowed that antigen-presenting ability of macrophages were significantly reduced afteroverexpression of miR-210. Assay of luciferase reporter gene expression demonstratedthat transfection of miR-210 mimics significantly inhibited

关键词： MicroRNA-210 Notch

Metallophthalocyanines-Catalyzed Aerobic C(sp2)-H Functionalization for C-N Bond Formation: efficient synthesis of 3,5-disubstituted 1H-pyrazoles

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Metallophthalocyanines-Catalyzed Aerobic C(sp2)-H Functional...

中国化学会第九届全国有机化学学术会议

作者： Qiu Jun Hua Chengwen Chen Bang zhao junlong Gou Xiaofeng College of Chemistry & Materials Science Northwest University

Pyrazoles constitute an important class of heterocyclic compounds exhibiting a wide range of biological activities1]and they also act as vital building blocks of many pharmaceuticals and natural products2].The importa...

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Preliminary study on the function of Toxoplasma gondii α-amylase 1 and its protective immunity against acute and chronic toxoplasmosis

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Preliminary study on the function of Toxoplasma gondii α-am...

中国畜牧兽医学会兽医寄生虫学分会第一届青年科学家学术论坛

作者： Jing Yang Chenghang Yang Jiahui Qian junlong zhao Rui Fang State Key Laboratory of Agricultural Microbiology College of Veterinary MedicineHuazhong Agricultural University

As a vitalparasiticprotozoan,the energy metabolism of Toxoplasma gondii(***)bradyzoitesis different from tachyzoites,and the most typical feature is massive amylopectin accumulated in the cytoplasm of *** is supposed that the amylopectin granule may be an energy reserve for the transformation of cysts and oocysts into tachyzoites.a-amylase 1(a-amyl) is one of the enzymes involved in the digestion of the amylopectin,and may be phosphorylated by *** the present study,ME49△α-amy1 mutant was generated using CRISPR/CAS9-mediated homologous gene replacement system to explore the specific biological functions of a-amyl in amylopectin metabolic pathway,and then intracellular replication assay and plaque assay were used to measure the proliferation ability of tachyzoites in *** staining was performed to identify the starch synthesis of ME49△α-amy1 mutant,and the virulence and formation of brain cysts formation were *** results obtained from our study revealed that the deletion of the a-amyl gene had no significant effect on the reproduction and starch synthesis in vitro,but contributed to virulence attenuation and reduction of the formation of brain cysts in ***,a mouse model was established to explore the protective immunity of ME49△α-awy1 mutant,and demonstrated that ME49△α-amy1 mutant could provide short-term and long-term protection against acute and chronic infections that survival rates were nearly 100%.From the experimental results,it can be assumed that other enzymes in combination with a-amyl may be involved in digestion of the amylopectin,and ME49△α-amyl attenuated strain exert a superior protective effect on both acute and chronic infections,suggesting that ME49 Aa-amyl mutant may possess the possibility of potential vaccine candidate for toxoplasmosis.

关键词： Toxoplasma gondii α-amylase 1 amylopectin metabolism protection vaccine