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Differential transcriptional regulation of the NANOG gene in chicken primordial germ cells and embryonic stem cells

Journal of Animal Science and Biotechnology 2021年第3期12卷 877-890页

作者： hee jung choi So Dam Jin Deivendran Rengaraj Jin Hwa Kim Bertrand Pain Jae Yong Han Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences Seoul National UniversitySeoul 08826South Korea Univ Lyon UniversitéLyon 1INSERMINRAEStem Cell and Brain Research InstituteU1208USC136169500 BronFrance Institute for Biomedical Sciences Shinshu UniversityMinamiminowaNagano 399-4598Japan

Background:NANOG is a core transcription factor(TF)in embryonic stem cells(ESCs)and primordial germ cells(PGCs).Regulation of the NANOG gene by TFs,epigenetic factors,and autoregulatory factors is well characterized in ESCs,and transcriptional regulation of NANOG is well established in these *** NANOG plays a key role in germ cells,the molecular mechanism underlying its transcriptional regulation in PGCs has not been ***,we investigated the mechanism that regulates transcription of the chicken NANOG(cNANOG)gene in PGCs and ***:We first identified the transcription start site of cNANOG by 5′-rapid amplification of cDNA ends PCR ***,we measured the promoter activity of various 5′flanking regions of cNANOG in chicken PGCs and ESCs using the luciferase reporter *** expression required transcriptional regulatory elements,which were positively regulated by POU5F3(OCT4)and SOX2 and negatively regulated by TP53 in *** proximal region of the cNANOG promoter contains a positive transcriptional regulatory element(CCAAT/enhancer-binding protein(CEBP)-binding site)in ***,small interfering RNA-mediated knockdown demonstrated that POU5F3,SOX2,and CEBP played a role in cell type-specific transcription of ***:We show for the first time that different trans-regulatory elements control transcription of cNANOG in a cell type-specific *** finding might help to elucidate the mechanism that regulates cNANOG expression in PGCs and ESCs.

关键词： Chicken Embryonic stem cells NANOG gene Primordial germ cells Regulatory elements

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Extracts from Paeonia lactiflora Pallas, Rehmannia Glutinosa var. Purpurea Makino, Perilla Frutescens var. Acuta Kudo may increase the endometrial receptivity through expression of leukemia inhibitory factor and adhesion molecules

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Journal of Traditional Chinese Medicine 2019年第1期39卷 15-25页

作者： Eun-Yeong Kim Tae-Wook Chung hee-jung choi Yeon-Seop jung Syng-Ook Lee Jun-Yong choi Hyung Sik Kim Sooseong You Myeong Soo Lee Ki-Tae Ha School of Korean Medicine and Korean Medical Research Center for Healthy Aging Pusan National UniversityYangsan 50612Republic of Korea Graduate Training Program of Korean Medicine for Healthy-aging Pusan National UniversityYangsan 50612Republic of Korea Department of Food Science and Technology Keimyung UniversityDaegu 42601Republic of Korea National Clinical Research Center for Korean Medicine Korean Medicine Hospital of Pusan National UniversityYangsan 50612Republic of Korea Laboratory of Molecular Toxicology School of PharmacySungKyunKwan UniversitySuwon 16419Republic of Korea Korea Institute of Oriental Medicine Daejeon 34054Republic of Korea

OBJECTIVE: To find out the combination of the extracts from Paeonia lactiflora Pallas(PL), Rehmannia Glutinosa var. Purpurea Makino(RG), Perilla Frutescens var. Acuta Kudo(PF) to increase endometrial ***: Herbal medicines were extracted with boiling water and polysaccharides were *** examined the effect of PL, RG, and PF(PRP), a most effective herbal formula deduced from constitutive ingredient herbs of Antai Yin which is composed of PRP, on the leukemia inhibitory factor(LIF) expression and endometrial ***: The combination of the extracts from PRP induced the LIF expression in Ishikawa cells and increased the adhesion between Ishikawa and JAr cells. In addition, PRP-induced attachment of JAr cells onto Ishikawa cells and expression of adhesion molecules, ITGAV, ITGB5, CD44 s, and Lselectin, are significantly reduced by knock-down of LIF ***: Induced by the combination of the PRP extracts, the adhesion between trophoblast and endometrial cells are mediated by expression of LIF and adhesion molecules. Thus, we suggest the combination of the PRP extracts may be a novel therapy for enhancing embryo implantation rate.

关键词： Paeonia Rehmannia Perilla Embryo implantation Endometrium Leukemia inhibitory factor Cell adhesion molecules

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Opuntia humifusa aqueous extract alleviates ethanol-induced gastric ulcer in a mouse model

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Asian Pacific Journal of Tropical Biomedicine 2020年第9期10卷 403-410页

作者： Chi-Yeol Yoo Hyeong-U Son Alshammari Fanar hee-jung choi Md Badrul Alam Sang-Han Lee Department of Food Science and Biotechnology Kyungpook National UniversityDaegu 41566Korea Food and Bio-Industry Research Institute Kyungpook National UniversityDaegu 41566Korea

Objective:To investigate the effect of Opuntia humifusa aqueous extract on gastric ***:An ethanol-induced model was used to examine the protective effect of Opuntia humifusa against gastric *** gastric ulcer index was evaluated via clinical observation and image *** inflammatory indicators were determined by RTPCR and Western blotting ***:The gastric ulcer index was reduced to 8%in the group treated with Opuntia humifusa aqueous extract compared with that in the control ***-PCR analysis revealed that MUC5 AC expression was reduced to 39%in the control group compared with the non-treated group,whereas the omeprazole and Opuntia humifusa aqueous extract-treated groups increased the expression to 95%and 79%,***,the expressions of various cytokines including TNF-α,IL-1β,and IL-6 were increased in the control group,while decreasing in Opuntia humifusa aqueous extract-treated *** humifusa aqueous extract also suppressed the expressions of iN OS,COX-2,and its transcription factor NF-κB and increased mucus content considerably as compared to the control ***:These results suggest that Opuntia humifusa aqueous extract is suitable as an alternative remedy for gastric ulcer treatment.

关键词： Opuntia humifusa Gastric ulcer Gastric lesions Image analysis Mucin

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Endovascular treatment of traumatic renal artery pseudoaneurysm with a Stanford type A intramural haematoma:A case report

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World Journal of Clinical Cases 2022年第27期10卷 9760-9767页

作者： Yook Kim Jin Young Lee Jin Suk Lee Jin Bong Ye Se Heon Kim Young Hoon Sul Su Young Yoon jung hee choi Hanlim choi Department of Radiology Chungbuk National University HospitalCheongju-si 28644Chungcheongbuk-doSouth Korea Department of Trauma Surgery Chungbuk National University HospitalCheongju-si 28644Chungcheongbuk-doSouth Korea Department of Trauma Surgery College of MedicineChungbuk National UniversityCheongju-si 28644Chungcheongbuk-doSouth Korea Cardiovascular and Thoracic Surgery Chungbuk National University HospitalCheongju-si 28644Chungcheongbuk-doSouth Korea Anesthesiology and Pain Medicine Chungbuk National University HospitalCheongju-si 28644Chungcheongbuk-doSouth Korea Department of Surgery Chungbuk National University HospitalCheongju-si 28644Chungcheongbuk-doSouth Korea

BACKGROUND Aortic intramural hematoma(IMH) associated with aortic branch tear and intramurally located pseudoaneurysm after blunt trauma has not been ***,we report a case of progressive type A aortic IMH associated with a pseudoaneurysm arising from the injured proximal renal artery after blunt *** SUMMARY During logging operations,a 66-year-old man experienced blunt force trauma after being injured by a fallen *** arrived at our trauma center with a left flank pain *** tomography(CT) revealed a pseudoaneurysm arising from the proximal renal artery(localized within the aortic media) and Stanford type A IMH.A covered stent was deployed along the left main renal artery,bridging the pseudoaneurysm and covering the parent artery,successfully excluding the pseudoaneurysm as confirmed using ***,although the degree of the pseudoaneurysm decreased,follow-up CT revealed remnant pseudoaneurysm,likely caused by an ***,a covered stent was additionally installed through the previously deployed covered *** exclusion of the pseudoaneurysm was confirmed using final *** the 7-mo follow-up CT scan,the IMH and pseudoaneurysm completely disappeared with no evidence of stent-related *** Endovascular treatment such as stent-graft placement can be an effective and safe treatment for traumatic renal artery injury.

关键词： Aortic intramural hematoma Blunt trauma Intimomedial tear Renal arterial injury Stent-graft Case report

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Production of germline chimeric quails following spermatogonial cell transplantation in busulfan-treated testis

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Asian Journal of Andrology 2018年第4期20卷 414-416页

作者： Young Min Kim Jin Se Park Jong Won Yoon hee jung choi Kyung Je Park Tamao Ono Jae Yong Han Department of Agricultural Biotechnology Research Institute of Agriculture and Life Sciences College of Agriculture and Life Sciences Seoul National University Seou108826 Korea Division of Animal Science Faculty of Agriculture Shinshu University Minamiminowa Nagano 399-4598 Japan Institute for Biomedical Sciences Shinshu University Minamiminowa Nagano 399-4598 Japan

Dear Editor, The merits of using quaff as an avian experimental model include high egg production, low maintenance cost, small body size, and short generation period （approximately 6-8 weeks）. Indeed, these characteristics make quail an ideal species for many biological areas including transgenic research. Methods of primordial germ cell （PGC）-mediated germline chimera production are considered very reliable and have been used in avian transgenesis.

关键词： COTURNIX-JAPONICA TRANSGENIC QUAIL TESTICULAR CELLS JAPANESE-QUAIL STEM-CELLS SPERMATOGENESIS TRANSMISSION CHICKEN

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ELK3-ID4 axis governs the metastatic features of triple negative breast cancer

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Oncology Research 2024年第1期32卷 127-138页

作者： JIN-HO choi JOO DONG PARK SEUNG hee choi EUN-SU KO HYE jung JANG KYUNG-SOON PARK Department of Biomedical Science CHA UniversitySeongnamKorea

Purpose:Cancer cell metastasis is a multistep process,and the mechanism underlying extravasation remains ***3 is a transcription factor that plays a crucial role in regulating various cellular processes,including cancer *** on the finding that ELK3 promotes the metastasis of triple-negative breast cancer(TNBC),we investigated whether ELK3 regulates the extravasation of TNBC by forming the ELK3-ID4 ***4 functions as a transcriptional regulator that interacts with other transcription factors,inhibiting their activity and subsequently influencing various biological processes associated with cell differentiation,survival,growth,and ***:We assessed the correlation between the expression of ELK3 and that of ID4 in TNBCs using bioinformatics analyses,QRT-PCR,western blot analysis,luciferase reporter assays,and chromatin ***,adhesion,invasion,and lung metastasis assays were employed to determine whether the ELK3-ID4 axis regulates the metastatic features of ***:We found that ELK3 binds directly to a binding motif close to the ID4 promoter to repress promoter *** expression of E-cadherin in TNBC was regulated by the ELK3-ID4 *** vitro and in vivo analyses showed that inhibiting ID4 expression in ELK3-knockdown MDA-MB-231(ELK3KD)cells restored the ability to extravasate and ***:The results indicate that the ELK3 regulates ID4 promoter activity,and that the ELK3-ID4 axis regulates the metastatic characteristics of TNBC ***,the data suggest that the ELK3-ID4 axis regulates metastasis of TNBCs by modulating expression of E-cadherin.

关键词： E-cadherin Extravasation Colonization

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Correlation between hydration properties and electrochemical performances on Ln cation size effect in layered perovskite for protonic ceramic fuel cells

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Journal of Energy Chemistry 2024年第1期88卷 1-9,I0001页

作者： Inhyeok Cho Jiwon Yun Boseok Seong Junseok Kim Sun hee choi Ho-Il Ji Sihyuk choi Department of Mechanical Engineering Kumoh National Institute of TechnologyGumi 39177GyeongbukRepublic of Korea Department of Aeronautics Mechanical and Electronic Convergence EngineeringKumoh National Institute of TechnologyGumi 39177GyeongbukRepublic of Korea Center for Energy Materials Research Korea Institute of Science and Technology(KIST)Seoul 02792Republic of Korea Center for Hydrogen and Fuel Cell Research Clean Technology Research DivisionKorea Institute of Science and Technology(KIST)Seoul 02792Republic of Korea Department of Energy and Environmental Engineering University of Science and TechnologySeoul 02792Republic of Korea Nanomaterials Science and Engineering Korea University of Science and Technology(UST)KIST CampusSeoul 02792Republic of Korea

PrBa_(0.5)Sr_(0.5)Co_(1.5)Fe_(0.5)O_(5+δ)(PrBSCF) has attracted much research interest as a potential triple ionic and electronic conductor(TIEC) electrode for protonic ceramic fuel cells(PCFCs). The chemical formula for Pr BSCF is AA'B_(2)O_(5+δ), with Pr(A-site) and Ba/Sr(A'-site) alternately stacked along the c-axis. Due to these structural features, the bulk oxygen ion diffusivity is significantly enhanced through the disorder-free channels in the PrO layer;thus, the A site cations(lanthanide ions) play a pivotal role in determining the overall electrochemical properties of layered perovskites. Consequently, previous research has predominantly focused on the electrical properties and oxygen bulk/surface kinetics of Ln cation effects,whereas the hydration properties for PCFC systems remain unidentified. Here, we thoroughly examined the proton uptake behavior and thermodynamic parameters for the hydration reaction to conclusively determine the changes in the electrochemical performances depending on LnBa_(0.5)Sr_(0.5)Co_(1.5)Fe_(0.5)O_(5+δ)(LnBSCF,Ln=Pr, Nd, and Gd) cathodes. At 500 ℃, the quantitative proton concentration of PrBSCF was 2.04 mol% and progressively decreased as the Ln cation size decreased. Similarly, the Gibbs free energy indicated that less energy was required for the formation of protonic defects in the order of Pr BSCF < Nd BSCF < Gd BSCF. To elucidate the close relationship between hydration properties and electrochemical performances in LnBSCF cathodes, PCFC single cell measurements and analysis of the distribution of relaxation time were further investigated.

关键词： Protonic ceramic fuel cell Cathode Triple ionic and electronic conductor Hydration property Proton uptake Gibbs free energy

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Omnidirectional color wavelength tuning of stretchable chiral liquid crystal elastomers

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Light(Science & Applications) 2024年第6期13卷 1062-1074页

作者： Seungmin Nam Wontae jung Jun Hyuk Shin Su Seok choi Department of Electrical Engineering Pohang University of Science and Technology(POSTECH)PohangKorea

Wavelength-tunable structural colors using stimuli-responsive materials,such as chiral liquid crystals(CLCs),have attracted increasing attention owing to their high functionality in various tunable photonic ***,on-demand omnidirectional wavelength control is highly desirable from the perspective of wavelength-tuning ***,despite numerous previous research efforts on tunable CLC structural colors,only mono-directional wavelength tuning toward shorter wavelengths has been employed in most studies to *** this study,we report the ideally desired omnidirectional wavelength control toward longer and shorter wavelengths with significantly improved tunability over a broadband wavelength *** using areal expanding and contractive strain control of dielectric elastomer actuators(DEAs)with chiral liquid crystal elastomers(CLCEs),simultaneous and omnidirectional structural color-tuning control was *** breakthrough in omnidirectional wavelength control enhances the achievable tuning freedom and versatility,making it applicable to a broad range of high-functional photonic applications.

关键词： tuning tunable directional

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Development of the Guideline for HIV Testing in South Korea with Low Prevalence of HIV-1 Infection Based on the Expert Consensus

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World Journal of AIDS 2011年第4期1卷 155-164页

作者： hee jung choi Kyoung Ae Kong jung Won Min Hun-Jae Lee Hae-Sook Park 不详

Purpose: This study was conducted to develop a guideline for HIV testing in South Korea where the HIV prevalence is very low. It is necessary to make the recommendations presented here based on the epidemiology of opportunistic infection and cancer in the community. Methods: The development of a guideline for HIV testing was conducted using literature reviews and the agreement of Korean experts for HIV patients using a two-round Delphi consensus technique. Each expert was asked to independently answer to each item related to HIV risk. Ten experts participated in the two-round survey. Results: The individual items were determined based on the following categories: high risk behavior, symptom and diseases, colo-rectal diseases, uro-genital diseases and gynecology, skin and oral diseases. The final guideline consisted of 4-scale agreement rating (strongly recommended, recommended, not routinely recommended, no recommendation for routine provision). Discussion: We have developed the guideline for HIV testing in a country where the HIV prevalence is low based on a systematic investigation and expert consensus.

关键词： HIV Testing Guideline Consensus Delphi Studies

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Health risks of pest control and disinfection workers after the COVID-19 outbreak in South Korea

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Journal of Environmental Sciences 2024年第5期139卷 350-363页

作者： Yun-hee choi Da-An Huh Lita Kim Sang ji Lee Kyong Whan Moon Department of Health and Safety Convergence Science Korea UniversityAnam-ro 145Seongbuk-guSeoul 02841South Korea BK21 FOUR R&E Center for Learning Health System Korea UniversityAnam-ro 145Seongbuk-guSeoul 02841South Korea Institute of Sciences Korea UniversityAnam-ro 145Seongbuk-guSeoul 02841South Korea Department of Health and Environmental Science Korea UniversityAnam-ro 145Seongbuk-guSeoul 02841South Korea

The exposure patterns of pest control and disinfection workers have changed after the coronavirus disease 2019(COVID-19)outbreak,but the health risks of chemical exposure have not been *** identified these workers’chemical exposure patterns and risks before and after the COVID-19 *** used data conducted between 2018(pre-pandemic)and 2021(post-pandemic)from three-year cross-sectional surveys on pest control and disinfection *** and dermal exposure concentrations were estimated using equations based on a biocidal product risk assessment model from the Korean National Institute of Environmental *** non-carcinogenic and carcinogenic risks of chemicals were calculated using the United States Environmental Protection Agency risk assessment *** found that the annual work frequency(50^(th)percentile)of foggers using disinfectants increased the most among all the work types,from 140 uses/year to 176 uses/year after the COVID-19 ***,all chemicals’non-carcinogenic and carcinogenic risks increased regardless of exposure *** the worst scenario(95^(th)percentile),the margin of exposure for citric acid,benzethonium chloride,benzyl-C12-16-alkyldimethyl chlorides,and sodium chlorite of inhalation exposure,and isopropyl alcohol and benzyl-C12-16-alkyldimethyl chlorides of dermal exposure were acceptable(>100)before the COVID-19outbreak but became unacceptable(10^(-6))before and after the COVID-19 outbreak but comparatively high after the COVID-19 ***,significantly more workers experienced health symptoms after the COVID-19outbreak(p<0.05),with the most common being muscle lethargy(31%),skin/face stinging(28.7%),and breath shortness/neck pain(24.1%).

关键词： Biocide Disinfection Health risk assessment Occupational exposure Pest control

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