Differential transcriptional regulation of the NANOG gene in chicken primordial germ cells and embryonic stem cells

作     者：Hee Jung Choi So Dam Jin Deivendran Rengaraj Jin Hwa Kim Bertrand Pain Jae Yong Han Hee Jung Choi;So Dam Jin;Deivendran Rengaraj;Jin Hwa Kim;Bertrand Pain;Jae Yong Han

作者机构：Department of Agricultural Biotechnology and Research Institute of Agriculture and Life SciencesSeoul National UniversitySeoul 08826South Korea Univ LyonUniversitéLyon 1INSERMINRAEStem Cell and Brain Research InstituteU1208USC136169500 BronFrance Institute for Biomedical SciencesShinshu UniversityMinamiminowaNagano 399-4598Japan 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2021年第12卷第3期

页      面：877-890页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 

基　　金：This work was supported by a National Research Foundation of Korea(NRF)grant funded by the Korea government(MSIP)[2015R1A3A2033826]and[2018R1D1A1B07049376] 

主　　题：Chicken Embryonic stem cells NANOG gene Primordial germ cells Regulatory elements 

摘      要：Background:NANOG is a core transcription factor(TF)in embryonic stem cells(ESCs)and primordial germ cells(PGCs).Regulation of the NANOG gene by TFs,epigenetic factors,and autoregulatory factors is well characterized in ESCs,and transcriptional regulation of NANOG is well established in these *** NANOG plays a key role in germ cells,the molecular mechanism underlying its transcriptional regulation in PGCs has not been ***,we investigated the mechanism that regulates transcription of the chicken NANOG(cNANOG)gene in PGCs and ***:We first identified the transcription start site of cNANOG by 5′-rapid amplification of cDNA ends PCR ***,we measured the promoter activity of various 5′flanking regions of cNANOG in chicken PGCs and ESCs using the luciferase reporter *** expression required transcriptional regulatory elements,which were positively regulated by POU5F3(OCT4)and SOX2 and negatively regulated by TP53 in *** proximal region of the cNANOG promoter contains a positive transcriptional regulatory element(CCAAT/enhancer-binding protein(CEBP)-binding site)in ***,small interfering RNA-mediated knockdown demonstrated that POU5F3,SOX2,and CEBP played a role in cell type-specific transcription of ***:We show for the first time that different trans-regulatory elements control transcription of cNANOG in a cell type-specific *** finding might help to elucidate the mechanism that regulates cNANOG expression in PGCs and ESCs.