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Identification and evolution-ary implication of four novel box H/ACA snoRNAs from Giardia lamblia

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Chinese Science Bulletin 2006年第20期51卷 2451-2456页

作者： LUO jun zhou hui CHEN Chongjian LI Yan CHEN Yueqin QU Lianghu Key Laboratory of Gene Engineering of the Ministry of Education StateKey Laboratory for Biocontrol Zhongshan University Guangzhou510275 China

From a specialized cDNA library, four novel box H/ACA snoRNAs, named GLsR22, GLsR23, GLsR24 and GLsR25, were identified from the primitive eukaryote, Giardia lamblia. Bioinformat- ics analyses indicated that all of them can be poten- tially folded into double hairpins, the typical secon- dary structures of box H/ACA snoRNAs. GLsR24 and GLsR25 are predicted to guide the site-specific pseudouridylation at U1753 and U2396 on 23S rRNA, respectively, while GLsR22 and GLsR23 belong to the family of “orphan” snoRNAs. All of the four novel snoRNAs are encoded by single copy genes and located in small intergenic regions. Interestingly, compared with the counterparts previously reported in Archaea and other unicellular protozoan, the box H/ACA snoRNAs identified from G. lamblia have unique structural features, implying that snoRNAs evolved from prokaryotes to eukaryotes in different ways.

关键词：贾第虫兰氏鞭毛虫基因工程核糖核酸

Photodegradation of α-naphthaleneacetic acid in aqueous solution

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Journal of Environmental Sciences 2001年第3期13卷 361-364页

作者： LIU hui jun, zhou Zhu fei, LIU Wei ping (Environmental Science Institute, Zhejiang University, Hangzhou 310027, China. Environmental Science Institute Zhejiang University Hangzhou 310027 China.

Kinetic processes of a-naphthaleneacetic acid (NAA) photolysis were studied under different conditions. The results showed that the ultraviolet light was more effective than fluorescent light in promoting degradation, and the degradation of NAA under ultraviolet light followed the first order kinetics with the photolysis rate constant of 1.15 x 10-2 min-1 and half-life time (t1/2) of 60 min. Further, it was proved that the photolysis rate was higher in the presence of oxygen, titanium dioxide (TiO2), and low pH (acidic solution). At last, two photolysis intermediates were identified by GC-MS and possible photolysis pathways were proposed.

关键词： Acids Degradation Fluorescence Photolysis Solutions

Rapidly counting atomic planes of ultra-thin MoSe2 nanosheets(1≤n≤4)on SiO2/Si substrate

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Rare Metals 2016年第8期35卷 632-636页

作者： Yi-Ping Wang hui-jun zhou Gui-Hua Zhao Tian-Long Xia Lei Wang Le Wang Li-Yuan Zhang Department of Physics Renmin University of ChinaBeijing 100872China Beijing Key Laboratory of Opto-electronic Functional Materialsand Micro-nano Devices Renmin University of China Department of Power and Electrical Engineering Northwest A&F UniversityYangling 712100ShaanxiChina

The optical, thermal and electrical properties of ultra-thin two-dimensional （2D） crystal materials are highly related to their thickness. Therefore, identifying the atomic planes of few-layer crystal materials rapidly is crucial to fundamental study. Here, a simple technique was demonstrated based on optical contrast for counting atomic planes （n） of few-layer MoSe2 on SiO2/Si substrates. It is found that the optical contrast of single-layer MoSe2 depends on light wavelength and thickness of SiO2 on Si substrate. The data calculated based on a Fresnel law-based model as well as atomic force microscopy （AFM） mea- surements fit well with the values measured by spectro- scopic ellipsometer. Furthermore, the calculated and measured contrasts were integral and plotted, which can be used to determine the MoSe2 atomic planes （1 ≤ n ≤ 4） accurately and rapidly.

关键词： Thickness identification Optical contrast MoSe2 Optical microscopy

Bioinformatics and Expression Analysis of NAC Transcription Factor Genes in Scutellaria baicalensis

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World Journal of Traditional Chinese Medicine 2018年第2期4卷 37-42页

作者： Juan Liu Ti-Ying Chen Yuan Yuan jun-hui zhou Yu-Yang Zhao Lu-Qi Huang Department in Chinese Academy of Chinese Medical Sciences State Key Laboratory Breeding Base of Dao-di HerbsChinese Academy of Chinese Medical Sciences

Background:NAC,as a unique transcription factor to plants,plays important roles in multiple biological functions,such as regulation of plant growth and development,hormone levels,and responses to various kinds of ***,there is a lack of research of NAC genes in Chinese ***:The study aimed to evaluate the potential functions of NAC genes in Scutellaria baicalensis by bioinformatics and expression analysis,and provide evidence of the molecular regulation mechanism involved in flavonoid biosynthesis in ***:The genes of NAC transcription factors in *** were obtained from cDNA library and their functions were explored using bioinformatic *** NAC genes were screened from the cDNA library of *** using BLAST comparison ***,the open reading frame(ORF) finder online tool was used to predict the full-length ORFs of NAC genes and their protein characteristics were explored by bioinformatic *** expression of NAC genes was then detected by quantitative polymerase chain reaction in different parts of *** and different leaves treated by gibberellin GA3 ***:Six genes of NAC transcription factors were cloned,two of which had complete *** genes cloned in this study were mainly expressed in the flowers of *** expression levels of NAC2,NAC3,NAC4,NAC5,NAC6 were increased firstly and then decreased gradually after 100 μM GA3 ***,some NACs and PAL2 in *** showed strong ***:This study suggested that NACs cloned in this study were mainly regulated the flavonoid biosynthesis in the flowers of ***;NAC6 in *** might be involved in the regulation of PAL2 transcription and affected the accumulation of flavonoids in the root of *** results provided a basis for further understanding the molecular regulation mechanism of flavonoid biosynthesis in ***.

关键词： Bioinformatics expression analysis NAC transcription factors Scutellaria baicalensis

GC-MS STUDY ON DIURETICS IN URINE Ⅱ.DETECTION METHOD USING TRIMETHYLSILYLATION

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Chinese Chemical Letters 1991年第1期2卷 19-22页

作者： Ya Wei LI jun LI Tong hui zhou Institute of Materia Medica,Chinese Academy of Medical Sciences Beijing 100050,China

A reliable method is described for the detection of amitoride,triamterene. canrenone and spironolactone in human urine using GC-MS analysis after *** mass spectrometric and metabotic features of the compounds are disc... 详细信息

关键词： mode GC-MS STUDY ON DIURETICS IN URINE DETECTION METHOD USING TRIMETHYLSILYLATION OC MS

SCC-UEFAS, an urban-ecological-feature based assessment system for sponge city construction

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Environmental Science and Ecotechnology 2022年第4期12卷 8-17页

作者： Zi-Tong Zhao Hou-Ming Cheng Sheng Wang Hai-Yan Liu Zi-Ming Song jun-hui zhou Ji-Wei Pang Shun-Wen Bai Shan-Shan Yang Jie Ding Nan-Qi Ren State Key Laboratory of Urban Water Resource and Environment Harbin Institute of TechnologyHarbin150000China Central&Southern China Municipal Engineering Design and Research Institute Co. LtdWuhan430010China China Energy Conservation and Environmental Protection Group Beijing100089China

An effective sponge city construction evaluation system plays a crucial role in evaluating sponge city construction *** construction of a sponge city evaluation system still faces challenges related to incomplete index selection and unscientific weight *** studies have focused on the comprehensive assessment of sponge city construction in the early *** study constructed a scientific assessment indicator system and a quantitative indicator weight at all levels by literature review and statistical analysis methods from an objective *** demonstrate how to utilize our evaluation methods,three construction schemes randomly generated by MATLAB were evaluated under evaluation states of constant weight and variable weight,*** 3 had the highest score of 0.638 under the constant weight assessment,but it cannot practically be the final construction scheme due to the imbalance between *** to the constant weight assessment,a variable weight assessment can effectively balance the states of the evaluation index with changes in the decision *** the three schemes,Scheme 2 is the best choice with a value of 0.0355 under variable weight evaluation due to punishment and incentives in the variable weight *** concept of“punishing”a disadvantageous indicator and“motivating”an advantageous indicator increases the relative advantages of the indices,ultimately affecting the assessment results of schemes and leading to a more balanced *** study provides reasonable analysis and decision-making mechanisms to support decision-making and guide the scientific selection of a construction scheme.

关键词： Sponge city evaluation system Principal component analysis Factor analysis Variable weight theory

A functional study of low molecular weight IgM from patients with autoimmune disease

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Journal of Microbiology and Immunology 2006年第3期4卷 207-213页

作者： hui jun zhou XIAO PING YANG WAN SHENG CHEN PETER ROBERTS THOMSON HU JI XU Department of Laboratory Science the Ninth People＇s Hospital School of Medicine Shanghai fiaotongUniversity Shanghai P. R. China Department of Rheumatology and Immunology Department of Pharmacology Changzheng Hospitalthe Second Military Medical University Shanghai P. R. China Department of Immunology Allergy and Arthritis Flinders Medical Centre Australia

High levels of low molecular weight (LMW) IgM in certain diseases are associated with clinical and laboratory indices which reflect the severity of the disease. These associations suggest that LMW IgM may play an important role in the immunopathogenesis of these diseases. To further approach the question concerning the functional activity of LMW IgM in disease, a panel of LMW IgM and high molecular weight (HMW) IgM preparations with or without rheumatoid factor (RF) activity were used to investigate their antibody binding activity and their effector function. It was found that LMW IgM-RF and HMW IgM-RF had a similar binding capacity to Fc fragment as there was no significant difference in the affinity index between them. It further showed that the rate of activation and total amount of utilization of complement by LMW IgM and HMW IgM was similar, although the mean fluorescence of C3 deposition by IgM-RF and HMW IgM-RF was slightly higher than that of LMW IgM-RF and other control RF antibodies. However, the current study demonstrated that LMW IgM had strong neutrophil activating properties when compared with HMW IgM. These findings suggest that one mechanism of LMW IgM contributing to the immunopathogenesis of RA may be due to the formation of circulating immune complex ( CIC) by LMW IgM with subsequent activation of neutrophils. Whether LMW IgM has other functional activity in disease is unclear and needs further investigation.

关键词： Low molecular weight IgM Antoimmune diseases Neutrophil Rheumatoid factor

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PVD涂层刀具切削砂岩的磨损面积研究

组合机床与自动化加工技术 2017年第9期 59-61,65页

作者：杨小军周慧包头钢铁职业技术学院内蒙古包头014010

研究切削参数对PVD涂层刀具磨损面积的影响。利用正交试验进行切削试验,采用网格划分方法计算刀具磨损面积,分析刀具磨损面积与主轴转速、进给速度和切削深度之间的关系,根据试验结果得到:提高主轴转速能够降低刀具磨损面积,增加进给速... 详细信息

研究切削参数对PVD涂层刀具磨损面积的影响。利用正交试验进行切削试验,采用网格划分方法计算刀具磨损面积,分析刀具磨损面积与主轴转速、进给速度和切削深度之间的关系,根据试验结果得到:提高主轴转速能够降低刀具磨损面积,增加进给速度与切削深度将使刀具磨损面积增大。根据经验公式,利用最小二乘法建立刀具磨损面积预测模型,通过F检验可知模型具有较高的显著性,并得到切削参数中影响刀具磨损面积的主次关系为切削深度、主轴转速、进给速度。

关键词：刀具磨损面积切削参数网格划分方法

Rational modulation of fluorophosphate cathode by anionic groups to reduce the polarization behavior for fast-charging sodium-ion batteries

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Journal of Energy Chemistry 2025年第1期100卷 509-521页

作者： Xinyuan Wang Fan Zhang Xingyu zhou Qian Wang Changyu Liu Yangyang Liu hui Wang Xiaojie Liu Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education College of Chemistry & Materials Science Northwest University

Na3V2（PO4）2O2F （VP） is recognized as a promising cathode material for sodium-ion batteries due to its stable structural framework and high specific *** functional theory （DFT） and finite element simulations show that incorporating SO42-into VP decreases its band gap,lowers the migration energy barrier,and ensures a uniform Na+concentration gradient and stress distribution during charge and discharge ***,the average Na+diffusion coefficient of Na3V2（PO4）1.95（SO4）0.05O2F（VPS-1） is roughly double that of VP,leading to enhanced rate capability (80 C,75.5 mAh g-1) and cycling stability (111.0 mAh g-1capacity after 1000 cycles at 10 C current density) for ***-1 exhibits outstanding fast-charging capabilities,achieving an 80%state of charge in just 8.1 *** assembled VPS-1//SbSn/NPC full cell demonstrated stable cycling over 200 cycles at a high 5 C current,maintaining an average coulombic efficiency of 95.35%.

关键词： Anionic group modulation Polarization behavior Fast-charging Sodium-ion battery Fluorophosphate Cathode