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Cyclin Dl antisense oligodexoyneucleotides inhibits growth and enhances chemosensitivity in gastric carcinoma cells

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World Journal of Gastroenterology 2006年第11期12卷 1766-1769页

作者： Xiao-Ming Shuai Gao-Xiong Han guo-Bin Wang jun-hua Chen Department of Gastroenterologic Surgery Union HospitalTongji Medical College Huazhong University of Science and Technology Wuhan 430022 Hubei Province China

AIM： To examine the effects of cyclin D1 antisense oligodexoyneucleotides （ASODN） on growth and chemosensitivity of gastric carcinoma cell lines SGC7901 and its mechanism. METHODS： Phosphorothioate modified cyclin D1 ASODN was encapsulated by LipofectAMINE2000 （LF2000） and transfected into cells, the dose-effect curves and growth curves were observed. 5-FU, MTX, CDDP of different concentrations were given after transfecting cells with cyclin D1 ASODN for 24 h, the dose-effect responses were observed and IC50s were calculated. The mRNA expression of cyclin D1, thymidylate synthase （TS）, thymidine phosphorylase （TP） and dihydrofolate reductase （DHFR） was detected by reverse transcription-PCR （RTPCR） at 24 h and 48 h after transfection. RESULTS： Dose-dependent inhibitory effect was caused by cyclin D1 ASODN in SGC7901 cells. Transfecting gastric carcinoma cells with 0.2 μmol/L cyclin D1 ASODN for 24 h could inhibit growth significantly and reduce expression of cyclin D1 mRNA. Cyclin D1 ASODN could increase the chemosensitivity to 5-FU, MTX, CDDP in cells, The IC50s of different chemotherapeutic agents in ASODN plus chemotherapy groups were significantly lower than those in controls. Transfection with cyclin D1 ASODN leaded to an increase in TS and DHFR mRNA and a decrease in TP mRNA as determined by RT-PCR at 24 h, the alterations were more significant at 48 h. CONCLUSIONS： Cyclin D1 ASODN can decrease mRNA expression of cyclin D1, inhibit growth and enhance the chemosensitivity by changing the expression of enzymes related to metabolism of chemotherapeutic agents in SGC7901 gastric carcinoma cells.

关键词： Gastric carcinoma Cyclin D1 Antisense oligodexoyneucleotides Chemosensitivity

Identification of bis-benzylisoquinoline alkaloids as SARS-CoV-2 entry inhibitors from a library of natural products

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Signal Transduction and Targeted Therapy 2021年第4期6卷 1113-1115页

作者： Chang-Long He Lu-Yi huang Kai Wang Chen-Jian gu Jie Hu gui-Ji Zhang Wei Xu You-hua Xie Ni Tang Ai-Long huang Key Laboratory of Molecular Biology for Infectious Diseases(Ministry of Education) Institute for Viral HepatitisDepartment of Infectious DiseasesThe Second Affiliated HospitalChongqing Medical UniversityChongqingChina Key Laboratory of Medical Molecular Virology(MOE/NHC/CAMS) Department of Medical Microbiology and ParasitologySchool of Basic Medical SciencesShanghai Medical CollegeFudan UniversityShanghaiChina Children's Hospital Fudan UniversityShanghaiChina

Dear Editor,Coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is a major public health *** spike(S)protein mutation D614G became dominant in SARS-CoV-2 during a global pandemic,which displayed increased infectivity.1 Entry of a virus into host cells is one of the most critical steps in the viral life *** blockade of the entry process is a promising therapeutic option for COVID-19,research attention has been focused on the discovery of viral entry *** SARS-CoV-2 entry inhibitor development is very attractive,no candidates have progressed into clinical trials yet.

关键词： acute respiratory attractive

Case for abdominal migraine in child patient treated by acupuncture

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World Journal of Acupuncture-Moxibustion 2020年第4期30卷 311-313页

作者： Dong-mei gu Jian-hua SUN Affiliated Hospital of Nanjing University of Chinese Medicine Nanjing 210029China

A girl patient,seven years old,complaining intermittent periumbilical pain accompanied with headache and vomiting for almost one *** was adopted in *** were Bǎihui(百会GV20),Yintang(印堂EX-HN3),Zhongwan(中脘CV12),Tàiyáng(太阳EX-HN5),Wangu(完骨GB12),Hegu(合谷LI4),Tianshū(天枢ST25),Zusānli(足三里ST36),Sānyinjiao(三阴交SP6)and TAichong(太冲LR3),electroacupuncture,warming needling moxibustion and infrared irradiation were *** and moxibustion were given once every two days and 10 treatments were as one *** one course of treatment,the child patient narrated that the attack frequency of headache and abdominal pain was reduced,the severity alleviated and the persistent duration *** two courses of treatment,abdominal pain never occurred and the child patient was recovered and had a normal life and school *** disease was not recurred in one-year follow-up.

关键词： Acupuncture Electroacupuncture Abdominal migraine Brain-gut axis

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车辆动力学仿真的可信度评估方法研究

北京理工大学学报 2015年第1期35卷 44-47页

作者：李军苑士华北京理工大学机械与车辆学院北京100081 中国兵器科学研究院北京100089

针对我国车辆动力学仿真中缺少可信度评估方法的问题,通过系统分析车辆动力学仿真特点和其他领域可信度评估方法,提出了基于相似度概念,综合应用层次分析法、模糊综合评判法的可信度评估方法,通过对仿真数据与试验数据的离散处理,以及... 详细信息

针对我国车辆动力学仿真中缺少可信度评估方法的问题,通过系统分析车辆动力学仿真特点和其他领域可信度评估方法,提出了基于相似度概念,综合应用层次分析法、模糊综合评判法的可信度评估方法,通过对仿真数据与试验数据的离散处理,以及对其相似元数、每个相似元的相似度、各相似元对系统相似度影响的权系数等分析计算,得到仿真可信度值,实现了车辆动力学仿真可信度的定量评估.

关键词：动力学仿真可信度评估

Clinical significance of PHPT1 protein expression in lung cancer

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Chinese Medical Journal 2010年第22期123卷 3247-3251页

作者： XU An-jian XIA Xiang-hou DU Song-tao gu jun-chao Beijing Tropical Disease Institute Beijing Friendship Hospital Capital Medical University Beijing 100050 China Department of General Surgery Beijing Friendship Hospital Capital Medical University Beijing 100050 China

Background In our previous studies, we found the expression of 14-kD phosphohistidine phosphatase （PHPT1） was associated with lung cancer cells migration and invasion, and PHPT1 mRNA expression level in lung cancer tissues clinically correlated with lymph node metastasis. In the present study, we aimed to further investigate the expression of PHPT1 protein in lung cancer. Methods Expression of PHPT1 protein in tissue samples from 146 lung cancers and 30 normal tissues adjacent to lung cancers was assessed using immunohistochemical method. Fisher＇s exact test was used to analyze expression patterns of PHPT1 protein in these tissue types. Meanwhile, we studied the correlation between expression of PHPT1 protein and clinicopathological features in lung cancer. Results Significantly higher expression levels of PHPT1 protein were found in lung cancer samples （53.42%） than in normal tissues adjacent to lung cancer （23.33%）（P=0.003）. Fisher＇s exact test showed that lung cancer stage positively correlated with expression of PHPT1 protein （P=-0.02）, and lung cancer samples with lymph node metastasis showed higher PHPT1 protein expression （P=-0.016） than the samples without lymph node metastasis. Conclusions The results of this study agree with findings from our previous study of PHPT1 mRNA expression in lung cancer tissues, and strongly suggest that PHPT1 protein is closely associated with the carcinogenesis and metastasis of lung cancer. Thus, therapy targeting PHPT1 （inhibition or silencing） could be potentially benefited for lung cancer patients.

关键词： PHPT1 protein lung cancer immunohistochemistry

Development of UAV-based shot seeding device for rice planting

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International Journal of Agricultural and Biological Engineering 2022年第6期15卷 1-7页

作者： Wei Liu Shuaishuai Zou Xuelang Xu Qingyu gu Weizhuo He jun huang junhao huang Zichen Lyu Jianqin Lin Zhiyan Zhou Rui Jiang Xiwen Luo College of Engineering South China Agricultural University and Guangdong Laboratory for Lingnan Modern AgricultureGuangzhou 510642China guangdong Provincial Key Laboratory of Agricultural Artificial Intelligence(GDKL-AAI) Guangzhou 510642China guangdong Engineering Research Center for Agricultural Aviation Application(ERCAAA) Guangzhou 510642China Key Laboratory of Key Technology on Agricultural Machine and Equipment(South China Agricultural University) Ministry of EducationGuangzhou 510642China

In order to realize the high-quality row seeding operation with unmanned aerial vehicle(UAV)in paddy field,a shot seeding device that can sow five rows of pelleted rice seeds at the same time was *** shot seeding device mainly consists of an external grooved wheel seed metering device and five shot seeding *** designed external grooved wheel seed metering device can take the seeds out of the seed box and divide the seeds into five *** shot seeding module can accelerate the pelleted rice seeds to reduce the impact of the UAV wind on the direction of seed ***,an angle adjustment mechanism for the shot seeding module was used to change the row *** seed metering device test verified that when the speed of the seed metering wheel was 15 r/min,the coefficient of variation of the discharge rate of each row(C.V1)and total seed discharge rate stability(C.V2)were 1.70%and 1.04%,*** processing technique was used to test the UAV seeding *** distribution characteristics of seeds on the ground showed that the number of seeds in each row gradually increased from both sides to the middle in the width *** to the statistics,there were 60%-70%of the seeds in each row in the 100 mm width *** field test showed that when the working height was 1.5 m and the seeding quantity was 38.56 kg/hm^(2),the performance of sowing in rows was obvious,the deviation rate of seeding quantity was 1.89%.After 16 d of sowing,the seeds’emergence rate was stable,and the average emergence rate was 82.63%and the yield was 6775.50 kg/hm^(2).

关键词： unmanned aerial vehicle rice direct seeding row seeding pelleted rice seeds

Recent advances in interfacial modification of zinc anode for aqueous rechargeable zinc ion batteries

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能源化学:英文版 2023年第8期83卷 287-303,I0009页

作者： Qing Wen Hao Fu Ru-de Cui He-Zhang Chen Rui-Han Ji Lin-Bo Tang Cheng Yan Jing Mao Ke-hua Dai Xia-Hui Zhang jun-Chao Zheng School of Metallurgy and Environment Central South UniversityChangsha 410083HunanChina Engineering Research Center of the Ministry of Education for Advanced Battery Materials Central South UniversityChangsha 410083HunanChina School of Chemistry and Chemical Engineering Hunan University of Science and TechnologyXiangtan 411201HunanChina School of Mechanical Medical and Process EngineeringQueensland University of TechnologyBrisbaneQueensland 4001Australia School of Materials Science and Engineering Zhengzhou UniversityZhengzhou 450001HenanChina College of Chemistry Tianjin Normal UniversityTianjin 300387China School of Mechanical and Materials Engineering Washington State UniversityPullman 99164WashingtonUnited States

To tackle energy crisis and achieve sustainable development, aqueous rechargeable zinc ion batteries have gained widespread attention in large-scale energy storage for their low cost, high safety, high theoretical capacity, and environmental compatibility in recent years. However, zinc anode in aqueous zinc ion batteries is still facing several challenges such as dendrite growth and side reactions(e.g., hydrogen evolution), which cause poor reversibility and the failure of batteries. To address these issues, interfacial modification of Zn anodes has received great attention by tuning the interaction between the anode and the electrolyte. Herein, we present recent advances in the interfacial modification of zinc anode in this review. Besides, the challenges of reported approaches of interfacial modification are also ***, we provide an outlook for the exploration of novel zinc anode for aqueous zinc ion *** hope that this review will be helpful in designing and fabricating dendrite-free and hydrogenevolution-free Zn anodes and promoting the practical application of aqueous rechargeable zinc ion batteries.

关键词： Zinc ion batteries Zinc anode Interfacial modification Functional coating

New yellow Ba_(0.93)Eu_(0.07)Al_(2)O_(4) phosphor for warm-white light-emitting diodes through single-emitting-center conversion

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Light(Science & Applications) 2013年第1期2卷 226-233页

作者： Xufan Li John D Budai Feng Liu Jane Y Howe Jiahua Zhang Xiao-jun Wang Zhanjun gu Chengjun Sun Richard S Meltzer Zhengwei Pan College of Engineering University of GeorgiaAthensGA 30602USA Department of Physics and Astronomy University of GeorgiaAthensGA 30602USA Materials Science and Technology Division Oak Ridge National LaboratoryOak RidgeTN 37831USA State Key Laboratory of Luminescence and Applications Changchun Institute of OpticsFine Mechanics and PhysicsChinese Academy of SciencesChangchun 130033China Department of Physics Georgia Southern UniversityStatesboroGA 30460USA Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China X-ray Science Division Argonne National LaboratoryArgonneIL 60439USA

Phosphor-converted white light-emitting diodes for indoor illumination need to be warm-white(i.e.,correlated color temperature,4000 K)with good color rendition(i.e.,color rendering index.80).However,no single-phosphor,single-emitting-center-converted white light-emitting diodes can simultaneously satisfy the color temperature and rendition requirements due to the lack of sufficient red spectral component in the phosphors’emission ***,we report a new yellow Ba_(0.93)Eu_(0.07)Al_(2)O_(4) phosphor that has a new orthorhombic lattice structure and exhibits a broad yellow photoluminescence band with sufficient red spectral ***-white emissions with correlated color temperature,4000 K and color rendering index.80 were readily achieved when combining the Ba_(0.93)Eu_(0.07)Al_(2)O_(4) phosphor with a blue light-emitting diode(440–470 nm).This study demonstrates that warm-white light-emitting diodes with high color rendition(i.e.,color rendering index.80)can be achieved based on single-phosphor,single-emitting-center conversion.

关键词： luminescence single-phosphor-conversion warm-white light-emitting diode yellow phosphor

Estimation of Iron Physiological Requirement in Chinese Children using Single Stable Isotope Tracer Technique

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Biomedical and Environmental Sciences 2022年第8期35卷 681-687页

作者： ZHANG Yu LIU Xiao Bing REN Tong Xiang CAI Jie LU Jia Xi NIU Ran PIAO Jian hua YANG Li Chen WANG jun YANG Xiao guang Chinese Center for Disease Control and Prevention Beijing 102206China Gansu Center for Disease Control and Prevention Gansu 73000LanzhouChina Key Laboratory of Trace Element Nutrition MOHNational Institute for Nutrition and HealthChinese Center for Disease Control and PreventionBeijing 100050China National Institute of Metrology Bejing 100029China Department of General Surgery Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBejing 100730China

Objective This study is to obtain precise data on iron physiological requirements in Chinese children using single stable isotope *** Thirty boys(10.6±0.2 years)and 27 girls(10.4±0.2 years)were received oral 6 mg ^(57)Fe each day for 5 consecutive *** blood samples were subsequently drawn to examine the change of total iron concentration and ^(57)Fe abundance at day 0,14,28,60,90,180,360,450,540,630,*** iron physiological requirement was calculated by iron loss combined with iron circulation rate once ^(57)Fe abundance stabilized in human *** The iron physiological requirement was significantly lower in boys than those values in girls(16.88±7.12 vs.18.40±8.81μg/kg per day,P<0.05).Correspondingly,the values were calculated as 722.46±8.43μg/day for boys and 708.40±7.55μg/day for girls,*** nearly 10%iron absorption rate,the estimated average iron physiological requirement was 6.0 mg/day in boys and 6.2 mg/day in *** This study indicate that iron physiological requirement could require more daily iron intake in girls as compare with the values in boys having the same body *** findings would be facilitate to the new revised dietary reference intakes.

关键词： Iron absorption rate Physiological requirement Chinese children Stale isotope tracer Dietary reference intakes