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scRNA-seq reveals that origin recognition complex subunit 6 regulates mouse spermatogonial cell proliferation and apoptosis via activation of Wnt/β-catenin signaling

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Asian Journal of Andrology 2024年第1期26卷 46-56页

作者： Shi-Wei Liu Jia-Qiang Luo Liang-Yu Zhao Ning-Jing Ou Chao-Yang Yu-Xiang Zhang Hao-Wei Bai Hong-Fang Sun Jian-Xiong Zhang Chen-Cheng Yao Peng Li Ru-Hui Tian Zheng Li Zi-jue zhu Department of Andrology Center for Men's HealthUrologic Medical CenterShanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China State Key Lab of Reproductive Medicine Nanjing Medical UniversityNanjing 211166China Shanghai Key Lab of Reproductive Medicine Shanghai Jiao Tong University School of MedicineShanghai 200080China Department of Urology Guangdong Provincial Key Laboratory of Biomedical ImagingThe Fifth Affiliated HospitalSun Yat-sen UniversityZhuhai 519000China Department of Urology First Affiliated Hospital of Nanjing Medical UniversityNanjing 210029China

The regulation of spermatogonial proliferation and apoptosis is of great significance for maintaining *** single-cell RNA sequencing(scRNA-seq)analysis of the testis was performed to identify genes upregulated in *** scRNAseq analysis,we identified the spermatogonia upregulated gene origin recognition complex subunit 6(Orc6),which is involved in DNA replication and cell cycle regulation;its protein expression in the human and mouse testis was detected by western blot and *** explore the potential function of Orc6 in spermatogonia,the C18-4 cell line was transfected with control or Orc6 ***,5-ethynyl-2-deoxyuridine(EdU)and terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL)assays,flow cytometry,and western blot were used to evaluate its effects on proliferation and *** was revealed that ORC6 could promote proliferation and inhibit apoptosis of C18-4 *** RNA sequencing and bioinformatics analysis indicated that Orc6 was involved in the activation of wingless/integrated(Wnt)/β-catenin *** blot revealed that the expression ofβ-catenin protein and its phosphorylation(Ser675)were significantly decreased when silencing the expression of *** findings indicated that Orc6 was upregulated in spermatogonia,whereby it regulated proliferation and apoptosis by activating Wnt/β-catenin signaling.

关键词： cell proliferation ORCG scRNA-seq analysis spermatogonia Wnt/β-catenin signaling

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硝酸根添加剂提升钠金属电池循环稳定性

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电源技术 2024年第7期48卷 1161-1169页

作者：朱珏梁叔全曹鑫鑫中南大学材料科学与工程学院湖南长沙410083

钠金属电池由于金属钠具有的高比能量密度和低电极电势特性极具发展潜力,然而,传统的碳酸酯类电解液与钠金属之间持续反应,导致电池的循环稳定性能不理想。添加剂策略是一种提升电解液性能的简单有效策略,通过盐包盐策略成功制备了NaNO_... 详细信息

钠金属电池由于金属钠具有的高比能量密度和低电极电势特性极具发展潜力,然而,传统的碳酸酯类电解液与钠金属之间持续反应,导致电池的循环稳定性能不理想。添加剂策略是一种提升电解液性能的简单有效策略,通过盐包盐策略成功制备了NaNO_(3)作为添加剂的酯类电解液。DFT计算和NMR测试结果表明,NO_(3)^(-)将进入Na+溶剂化鞘层内侧,在钠金属负极优先还原。对于钠金属界面的SEM和XPS表征结果及电化学测试显示,NO_(3)^(-)的还原构建了含氮的SEI界面,助力均匀稳定的钠金属沉积剥离行为。Na_(3)V_(2)(PO_(4))_(3)(NVP)与钠金属负极组装的钠离子电池在NO_(3)^(-)改性的酯类电解液中可获得超长稳定循环,在2 C(1 C=110 mA/g)下循环了2000次后容量保持率高达89.9%,在5 C下循环1000次后容量保持率为97.4%。

关键词：钠金属电池酯类电解液硝酸根添加剂固态电解质界面

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热腐蚀和热冲击下炭化釜Q245R钢本构关系的研究

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高压物理学报 2024年第3期38卷 139-148页

作者：刘志远陈文飞谢作然蒋昊成李进朱珏宁波大学压力容器与管道安全浙江省工程研究中心浙江宁波315211 宁波大学冲击与安全工程教育部重点实验室浙江宁波315211 宁大•宏日UHPC力学联合研究院浙江宁波315211 宁波市特种设备检验研究院浙江宁波315211 宁波市特种设备智能检验与监测重点实验室浙江宁波315211 浙江省市场监管特种设备智能检验与监测重点实验室浙江宁波315211

采取电化学加速腐蚀试验对Q245R钢试样进行处理,以模拟炭化釜的实际腐蚀工况,通过电化学腐蚀拉伸试验,发现腐蚀不仅改变试样的几何尺寸,而且导致材料力学性能退化。对Q245R钢材料进行不同温度、腐蚀率、应变率(10^(-3)~1 s^(-1)低应变率... 详细信息

采取电化学加速腐蚀试验对Q245R钢试样进行处理,以模拟炭化釜的实际腐蚀工况,通过电化学腐蚀拉伸试验,发现腐蚀不仅改变试样的几何尺寸,而且导致材料力学性能退化。对Q245R钢材料进行不同温度、腐蚀率、应变率(10^(-3)~1 s^(-1)低应变率、10~10^(2) s^(-1)中应变率、10^(3) s^(-1)高应变率)的拉伸试验,并运用MATLAB在Johnson-Cook本构方程的基础上进行拟合,增加特征强度与热处理温度、腐蚀率的关系,从而确定了材料的本构关系。结果显示,本构曲线与真实拉伸试验数据吻合较好,拟合效果良好。

关键词：炭化釜 Q245R钢腐蚀 Johnson-Cook本构方程

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EQUIVALENT MODEL OF EXPANSION OF CEMENT MORTAR UNDER SULPHATE EROSION

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Acta Mechanica Solida Sinica 2008年第4期21卷 327-332页

作者： jue zhu Minqiang Jiang Jiankang Chen Mechanics and Materials Science Research Center Ningbo University Ningbo 315211 China State Key Laboratory of Nonlinear Mechanics （LNM） Institute of Mechanics Chinese Academy of Sciences Beijing 100080 China

The expansion property of cement mortar under the attack of sulfate ions is studied by experimental and theoretical methods. First, cement mortars are fabricated with the ratio of water to cement of 0.4, 0.6, and 0.8. Secondly, the expansion of specimen immerged in sulphate solution is measured at different times. Thirdly, a theoretical model of expansion of cement mortar under sulphate erosion is suggested by virtue of represent volume element method. In this model, the damage evolution due to the interaction between delayed ettringite and cement mortar is taken into account. Finally, the numerical calculation is results indicate that the model perfectly describes performed. The numerical and experimental the expansion of the cement mortar.

关键词： size effect microvoids expansion sodium sulfate attack

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TRANSVERSAL INERTIAL EFFECT ON RELAXATION/RETARDATION TIME OF CEMENT MORTAR UNDER HARMONIC WAVE

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Acta Mechanica Solida Sinica 2008年第1期21卷 44-50页

作者： jue zhu Yonghui Cao Jiankang Chen Mechanics and Materials Science Research Center Ningbo University Ningbo 315211 China

Under dynamic loading, the constitutive relation of the cement mortar will be significantly affected by the transversal inertial effect of specimens with large diameters. In this paper, one-dimensional theoretical analysis is carried out to determine the transversal inertial effect on the relaxation/retardation time of the cement mortar under the harmonic wave. Relaxation time or retardation time is obtained by means of the wave velocity, attenuation coefficient and the frequency of the harmonic wave. Thus, the transversal inertial effect on the relaxation time from Maxwell model, as well as on retardation time from Voigt model is analyzed. The results show that the transversal inertial effect may lead to the increase of the relaxation time, but induce the decrease of the retardation time. Those should be taken into account when eliminating the transversal inertial effect in applications.

关键词： cement mortar harmonic wave viscoelastic model relaxation/retardation time

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Underlying principle of efficient propulsion in flexible plunging foils

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Acta Mechanica Sinica 2014年第6期30卷 839-845页

作者： Xiao-jue zhu Guo-Wei He Xing Zhang LNM Institute of MechanicsChinese Academy of Sciences

Passive flexibility was found to enhance propulsive efficiency in swimming *** this study,we numerically investigate the roles of structural resonance and hydrodynamic wake resonance in optimizing efficiency of a flexible plunging *** results indicates that（1）optimal efficiency is not necessarily achieved when the driving frequency matches the structural eigenfrequency;（2）optimal efficiency always occurs when the driving frequency matches the wake resonant frequency of the time averaged velocity ***,the underlying principle of efficient propulsion in flexible plunging foil is the hydrodynamic wake resonance,rather than the structural *** addition,we also found that whether the efficiency can be optimized at the structural resonant point depends on the strength of the leading edge vortex relative to that of the trailing edge *** result of this work provides new insights into the role of passive flexibility in flapping-based propulsion.

关键词： Flexibility Propulsive efficiency Structural resonance Hydrodynamic wake resonance

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Transcriptome research on spermatogenic molecular drive in mammals

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Asian Journal of Andrology 2015年第6期17卷 961-971,I0009页

作者： Zi-jue zhu Shi Yang Zheng Li Department of Urology Ren Ji Hospital School of Medicine Shanghai Jiao Tong University Shanghai 200127 China Department of Andrology Urologic Medical CenterShanghai General Hospital Shanghai Jiao Tong University Shanghai 200080 China Shanghai Key Laboratory of Reproductive Medicine Shanghai 200025 China.

It is known that spermatogenic disorders are associated with genetic deficiency, although the primary mechanism is still unclear. It is difficult to demonstrate the molecular events occurring in testis, which contains germ cells at different developmental stages. However, transcriptomic methods can help us reveal the molecular drive of male gamete generation. Many transcriptomic studies have been performed on rodents by utilizing the timing of the first wave of spermatogenesis, which is not a suitable strategy for research in fertile men. With the development of separation methods for male germ cells, transcriptome research on the molecular drive of spermatogenesis in fertile men has seen great progress, and the results could be ultimately applied to improve the diagnosis and treatment for male infertility.

关键词： GeneChip germ cell RNA-Seq sorting spermatogenesis transcriptome

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EXPERIMENTAL EVALUATION ON MODULUS OF EQUIVALENT HOMOGENEOUS ETTRINGITE

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Acta Mechanica Solida Sinica 2009年第4期22卷 320-327页

作者： Minghua Zhang Jiankang Chen jue zhu Jiangying Chen Department of Mechanics and Engineering Science Ningbo University Ningbo 315211 China

In the present study, the average modulus of delayed ettringite is evaluated by an experimental method combined with theoretical analysis. Firstly, the delayed ettringite crystal is synthesized by chemical reaction of Aluminum sulfate and calcium hydroxide. Secondly, specimens are obtained by compressing the delayed ettringite crystal under different pre-loads. Thirdly, the variation of the modulus of the specimen with different pre-loads is tested using Instron material test machine and the SHPB technique, respectively. It is found that the experimental data may be suitably fitted by Boltzmann Function. Finally, the porosity of the specimen is detected using the saturation method, and the effect of the porosity on the modulus is analyzed by the Eshelby＇s equivalent inclusion method and the Mori-Tanaka＇s scheme. The static and dynamic modulli of the equivalent homogeneous ettringite obtained in present study are approximately 10.64 GPa and 24.61 GPa, respectively.

关键词： ettringite equivalent homogeneous materials Boltzmann Function modulus

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Effect of Indirect Transformation of Retained Austenite During Tempering on the Charpy Impact Toughness of a Low-Alloy Cr-Mo-V Steel

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Acta Metallurgica Sinica(English Letters) 2020年第10期33卷 1346-1358页

作者： Yong-Han Li Zhong-Hua Jiang Zhen-Dan Yang jue-Shun zhu Shenyang National Laboratory for Materials Science Institute of Metal ResearchChinese Academy of Sciences72 Wenhua RoadShenyang 110016China

A modified tempering treatment has been designed in order to avoid the direct transformation of retained austenite(Ar)during tempering of a low-alloy Cr-Mo-V *** of the direct transformation of Ar into ferrite and M23C6 carbides during conventional tempering at 700℃,transformation into aggregate of ferrite and cementite has been forced by a pre-tempering at 455℃ before conventional *** have been performed on specimens quenched with cooling rates 1.5,3 and 12℃/s,providing different types of Ar within the as-quenched *** results show that the tempering modification does not improve the Charpy impact toughness at the highest quenching rate of 12℃/s,where the specimens cannot incur cleavage cracking induced from fine and discontinuous M23C6 carbides along lath *** the lowest quenching rate 1.5℃/s,the Charpy impact toughness can be improved,and the failure is dominated by carbide aggregates,which originate from the decomposed products of blocky *** is because the tempering modification effectively suppresses the formation of coarse M23C6 carbides at interfaces between the carbide aggregate and bainitic matrix,thereby resulting in a relatively homogeneous distribution of M23C6 carbides inside carbide ***,the tempering modification is recommended for large-scale forgings,in which relatively high quenching rates are difficult to achieve.

关键词： Bainite steel Retained austenite Impact toughness

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DEVELOPMENT OF EXPERIMENTAL METHODS FOR IMPACT TESTING BY COMBINING HOPKINSON PRESSURE BAR WITH OTHER TECHNIQUES

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Acta Mechanica Solida Sinica 2014年第4期27卷 331-344页

作者： Lili Wang Shisheng Hu Liming Yang Zijian Sun jue zhu Huawei Lai Yuanyuan Ding Mechanics and Material Science Research Center Ningbo University Department of Modern Mechanics University of Science & Technology of China Huafeng Faculty Ningbo Polytechnic Department of Municipal Engineering Zhejiang University of Water Resources and Electric Power

The split Hopkinson pressure bar（SHPB） technique and the wave propagation inverse analysis（WPIA） technique are both extensively used to experimentally investigate the impact behavior of materials, although neither of them alone provides a fully satisfactory analysis. In the present paper, attention is given to new experimental techniques by incorporating a damagemodified constitutive model into the SHPB technique and combining the Hopkinson pressure bar（HPB） technique with WPIA. First, to distinguish the response due to dynamic constitutive behavior and the response due to dynamic damage evolution, the SHPB method incorporating a damage-modified constitutive model is developed, including an explicit damage-modified zhu–Wang–Tang model and an implicit damage-modified constitutive model. Second, when the SHPB results become invalid, a method of combining new Lagrange inverse analyses with the HPB technique is developed, including cases of the HPB arranged in front of a long specimen and behind the specimen. As examples of these new methods, typical results are given for nonlinear viscoelastic polymers and concretes considering damage evolution, a super-elastic Ti–Ni alloy with phase transformation and an aluminum foam with shock waves propagating within it.

关键词： Hopkinson pressure bar SHPB Lagrangian analysis high strain rate rate-dependent constitutive relation damage evolution

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