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Asymmetric cell division in plant development

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Journal of Integrative Plant Biology 2023年第2期65卷 343-370页

作者： Yi Zhang Tongda Xu juan dong Plant Synthetic Biology Center Haixia Institute of Science and Technologyand College of Life SciencesFujian Agriculture and Forestry UniversityFuzhou 350002 China The Waksman Institute of Microbiology Rutgersthe State University of New JerseyPiscatawayNJ 08854USA Department of Plant Biology Rutgersthe State University of New JerseyNew BrunswickNJ 08891USA

Asymmetric cell division(ACD) is a fundamental process that generates new cell types during development in eukaryotic *** plant development,post-embryonic organogenesis driven by ACD is universal and more important than in animals,in which organ pattern is preset during ***,plant development provides a powerful system to study molecular mechanisms underlying *** the past decade,tremendous progress has been made in our understanding of the key components and mechanisms involved in this important process in ***,we present an overview of how ACD is determined and regulated in multiple biological processes in plant development and compare their conservation and specificity among different model cell *** also summarize the molecular roles and mechanisms of the phytohormones in the regulation of plant ***,we conclude with the overarching paradigms and principles that govern plant ACD and consider how new technologies can be exploited to fill the knowledge gaps and make new advances in the field.

关键词： asymmetric cell division peptide signaling phytohormonal signaling polarity proteins plant development transcription factors

Study on the Interaction of Pd-TMPyP with ctDNA by Solid-substrate Room Temperature Phosphorescence

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Chinese Chemical Letters 2006年第2期17卷 253-254页

作者： Li Min GUO Wen juan dong Chuan dong School of Chemistry and Chemical Engineering Shanxi University Taiyuan 030006

The interaction between calf thymus DNA （ctDNA） and Pd （Ⅱ） meso-tetralds- （4-N-methyl pyridiniumyl） porphyrin （Pd-TMPyP） was investigated by steady solid-substrate room temperature phosphorescence （SS-RTP）. The SS-RTP intensity and lifetime of Pd-TMPyP enhanced with the increasing ctDNA. The anion quenching experiment of Pd-TMPyP indicated that Pd-TMPyP intercalated into ctDNA, which was also approved by UV-Vis spectra. Keywords： Pd-TMPyP, ctDNA, SS-RTP, intercalation

关键词： Pd-TMPyP ctDNA SS-RTP intercalation.

Cell polarity: Regulators and mechanisms in plants

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Journal of Integrative Plant Biology 2020年第1期62卷 132-147页

作者： Kezhen Yang Lu Wang Jie Le juan dong Key Laboratory of Plant Molecular Physiology CAS Center for Excellence in Molecular Plant SciencesInstitute of BotanyChinese Academy of SciencesBeijing 100093China Waksman Institute of Microbiology RutgersThe State University of New JerseyPiscatawayNew Jersey 08854.USA Department of Plant Biology RutgersThe State University of New JerseyPiscatawayNJ 08901USA

Cell polarity plays an important role in a wide range of biological processes in plant growth and *** polarity is manifested as the asymmetric distribution of molecules,for example,proteins and lipids,at the plasma membrane and inside of a ***,we summarize a few polarized proteins that have been characterized in plants and we review recent advances towards understanding the molecular mechanism for them to polarize at the plasma *** mechanisms,including membrane trafficking,cytoskeletal activities,and protein phosphorylation,and so forth define the polarized plasma membrane *** discoveries suggest that the polar positioning of the proteo-lipid membrane domain may instruct the formation of polarity complexes in *** this review,we highlight the factors and regulators for their functions in establishing the membrane asymmetries in plant ***,we discuss a few outstanding questions to be addressed to better understand the mechanisms by which cell polarity is regulated in plants.

关键词： polarity polarized instru

Global Helically Symmetric Solutions to 3D MHD Equations

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Acta Mathematicae Applicatae Sinica 2014年第2期30卷 347-358页

作者： Wen GAO Zhen-hua GUO dong-juan NIU Northwest University School of Mathematical Sciences Capital Normal University

We prove the existence and uniqueness of global strong solutions to the Cauchy problem of the three-dimensional magnetohydrodynamic equations in R3 when initial data are helically symmetric. Moreover, the large-time b... 详细信息

关键词： magnetohydrodynamic equations helically symmetric solutions large-time behavior of solutions

Isolated myeloid sarcoma in the pancreas and orbit:A case report and review of literature

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World Journal of Clinical Cases 2018年第11期6卷 477-482页

作者： Ting Zhu Xu-Yan Xi Hong-juan dong Department of Digestive Disease Weinan Central Hospital Department of Surgical Oncology Weinan Central Hospital Department of Haematology Xijing Hospitalthe Military Medical University of the PLA Air Force

Myeloid sarcoma(MS) is a type of extramedullary solid haematological tumour. Myeloid sarcoma is classified into two types based on whether onset of the disease is complicated by haematologic diseases: extramedullary infiltration of leukaemia(leukaemic MS) and isolated myeloid sarcoma. The incidence of isolated myeloid sarcoma is low. In particular, isolated myeloid sarcoma involving the pancreas is extremely rare and prone to misdiagnosis. This case report describes the long and eventful diagnostic process of a case of myeloid sarcoma involving the pancreas and orbit. Due to a lack of typical clinical manifestations and imaging characteristics, the patient underwent several rounds of treatment without a confirmed diagnosis. Eventually, the final diagnosis was pathologically confirmed using several types of biopsies and immunohistochemical detection. To date, this type of disease has not been reported in the literature. This case report describes the detailed diagnostic process and discusses the strategies used for diagnosis, which will facilitate the diagnosis of such diseases in the future.

关键词： Immunohistochemistry Isolated myeloid sarcoma Granulocytic sarcoma Pancreas Orbit

An analytical model for predicting sheet springback after V-bending

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2007年第2期8卷 237-244页

作者： ZHANG dong-juan CUI Zhen-shan CHEN Zhi-ying RUAN Xue-yu National Mold ＆ Die CAD Engineering Research Center Shanghai Jiao Tong University Shanghai 200030 China

Springback is caused by the redistribution of stress in sheet material after the tooling is removed. Precise prediction of sheet springback is very important in die design. Based on Hill’s yielding criterion and plane strain condition, an analytical model is proposed in this paper which takes into account the effects of contact pressure, the length of bending arm between the punch and die, transverse stress, neutral surface shifting and sheet thickness thinning on the sheet springback of V-bending. The predicted results by this analytical model indicated that the contact pressure and transverse stress have much effect on the springback when the bending ratio (the ratio of punch radius to sheet thickness) is less than five. The contact pressure declined when the length of bending arm goes up, which means that shorter length of bending arm will result in larger springback. The effect of neutral surface shifting on the springback is less than that of contact pressure and decreases with the bending ratio. However, this research showed that the influence of thickness thinning on the springback can be ignored. Comparison with finite element method (FEM) simu-lating results shows that the predicted results by the analytical model accord well with simulation results by FEM. In addition to that, the bending ability—the limit bending ratio for a given sheet thickness and material properties was also determined.

关键词： Springback V-bending Contact pressure Neutral surface shifting Transverse stress Bending ratio

Effect of electroacupuncture on glial fibrillary acidic protein and nerve growth factor in the hippocampus of rats with hyperlipidemia and middle cerebral artery thrombus

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Neural Regeneration Research 2021年第1期16卷 137-142页

作者： Na-Ying Xue dong-Yu Ge Rui-juan dong Hyung-Hwan Kim Xiu-Jun Ren Ya Tu School of Acupuncture-Moxibustion and Tuina Beijing University of Chinese MedicineBeijingChina School of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijingChina Neurovascular Research Laboratory Department of RadiologyMassachusetts General Hospital and Harvard Medical SchoolCharlestownMAUSA

Electroacupuncture(EA)has been shown to reduce blood lipid level and improve cerebral ischemia in rats with hyperlipemia complicated by cerebral ***,there are few studies on the results and mechanism of the effect of EA in reducing blood lipid level or promoting neural repair after stroke in hyperlipidemic *** this study,EA was applied to a rat model of hyperlipidemia and middle cerebral artery thrombosis and the condition of neurons and astrocytes after hippocampal injury was *** for the normal group,rats in other groups were fed a high-fat diet throughout the whole *** models were established in rats fed a high-fat diet for 6 *** cerebral artery thrombus models were induced by pasting 50%FeCl3 filter paper on the left middle cerebral artery for 20 minutes on day 50 as the model ***1 group rats received EA at bilateral ST40(Fenglong)for 7 days before the *** in the EA1 and EA2 groups received EA at GV20(Baihui)and bilateral ST40 for 14 days after model *** health was assessed by hematoxylin-eosin staining in the *** was assessed by biochemical methods that measured total cholesterol,triglyceride,low-density lipoprotein and high-density lipoprotein in blood *** analysis was used to confirm the establishment of the *** methods were used to detect the expression of glial fibrillary acidic protein and nerve growth factor in the hippocampal CA1 *** results demonstrated that,compared with the model group,blood lipid levels significantly decreased,glial fibrillary acidic protein immunoreactivity was significantly weakened and nerve growth factor immunoreactivity was significantly enhanced in the EA1 and EA2 *** repair effect was superior in the EA1 group than in the EA2 *** findings confirm that EA can reduce blood lipid,inhibit glial fibrillary acidic protein expression and promote nerve growth factor

关键词： astrocytes CA1 cerebral ischemia electroacupuncture glial fibrillary acidic protein hematoxylin-eosin staining hippocampus hyperlipidemia immunohistochemistry nerve growth factor

Characterization of a Novel Rice Metallothionein Gene Promoter:Its Tissue Specificity and Heavy Metal Responsiveness

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Journal of Integrative Plant Biology 2010年第10期52卷 914-924页

作者： Chun-juan dong Yun Wang Shi-Shi Yu Jin-Yuan Liu Laboratory of Molecular Biology and MOE Laboratory of Protein Science School of Life Sciences Tsinghua University Beijing 100084China

The rice （Oryza sativa L.） metallothionein gene OsMT-I-4b has previously been identified as a type I MT gene. To elucidate the regulatory mechanism involved in its tissue specificity and abiotic induction, we isolated a 1 730 bp fragment of the OsMT-I-4b promoter region. Histochemical /^-glucuronidase （GUS） staining indicated a precise spacial and temporal expression pattern in transgenic Arabidopsis. Higher GUS activity was detected in the roots and the buds of flower stigmas, and relatively lower GUS staining in the shoots was restricted to the trichomes and hydathodes of leaves. No activity was observed in the stems and seeds. Additionally, in the root of transgenic plants, the promoter activity was highly upregulated by various environmental signals, such as abscisic acid, drought, dark, and heavy metals including Cu2＋, Zn2~, Pb2~ and Al3＋. Slight induction was observed in transgenic seedlings under salinity stress, or when treated with Co2＋ and Cd2＋. Promoter analysis of 5＇-deletions revealed that the region -583/-1 was sufficient to drive strong GUS expression in the roots but not in the shoots. Furthermore, deletion analysis indicated important promoter regions containing different metal- responsive c/s-elements that were responsible for responding to different heavy metals. Collectively, these findings provided important insight into the transcriptional regulation mechanisms of the OsMT-I- 4b promoter, and the results also gave us some implications for the potential application of this promoter in plant genetic engineering.

关键词： PLANT gene expression PLANT genetics METALLOTHIONEIN PROMOTERS (Genetics) HEAVY metals PLANT genetic engineering TRANSGENIC rice ARABIDOPSIS PLANT cells & tissues GENETIC regulation in plants

Deformation Behavior of Ti-5.6Al-4.8Sn-2.0Zr-1.0Mo-0.35Si-0.85Nd Alloy in β/Quasi-β Forging Process

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Journal of Iron and Steel Research International 2016年第12期23卷 1297-1302页

作者： Ke-lu WANG Shi-qiang LU Xian-juan dong Xin LI De-lai OUYANG School of Aeronautical Manufacturing Engineering Nanchang Hangkong University

The hot deformation behavior of Ti 5.6Al-4.8Sn-2.0Zr-1.0Mo 0.35Si 0.85Nd alloy in β/quasi-β forging process was studied using isothermal compression tests over temperature range from 1040℃ to 1 100 ℃ and strain rates form 0. 001 s-1 to 70 s -1. The results show that the flow stress and mierostrueture are sensitive to thermomechanical parameters. The processing maps based on the dynamic materials model at strain of 0.3 and 0.7 were established. The optimum deformation thermomechanical parameters at a strain of 0.7 have two regions that exhibit the peak of power dissipation efficiency. One is the region of 1062-1100 ℃ and 10- 3 10-1.5 s -1 ; and another which represents dynamic recrystallization is 1040-1045 ℃ and 10-1.8 10- 0.5 s -1. The instable region is located where the strain rate is larger than 1 s 1 which corresponds to the mechanical instability.

关键词： titanium alloy β/quasi-β forging deformation behavior processing map process parametersoptim ization