Effect of electroacupuncture on glial fibrillary acidic protein and nerve growth factor in the hippocampus of rats with hyperlipidemia and middle cerebral artery thrombus

作     者：Na-Ying Xue Dong-Yu Ge Rui-Juan Dong Hyung-Hwan Kim Xiu-Jun Ren Ya Tu Na-Ying Xue;Dong-Yu Ge;Rui-Juan Dong;Hyung-Hwan Kim;Xiu-Jun Ren;Ya Tu

作者机构：School of Acupuncture-Moxibustion and TuinaBeijing University of Chinese MedicineBeijingChina School of Traditional Chinese MedicineBeijing University of Chinese MedicineBeijingChina Neurovascular Research LaboratoryDepartment of RadiologyMassachusetts General Hospital and Harvard Medical SchoolCharlestownMAUSA 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2021年第16卷第1期

页      面：137-142页

核心收录：

学科分类：1005[医学-中医学] 100512[医学-针灸推拿学] 10[医学] 

基　　金：This study was funded by the National Natural Science Foundation of China No.81470200(to XJR) 

主　　题：astrocytes CA1 cerebral ischemia electroacupuncture glial fibrillary acidic protein hematoxylin-eosin staining hippocampus hyperlipidemia immunohistochemistry nerve growth factor 

摘      要：Electroacupuncture(EA)has been shown to reduce blood lipid level and improve cerebral ischemia in rats with hyperlipemia complicated by cerebral ***,there are few studies on the results and mechanism of the effect of EA in reducing blood lipid level or promoting neural repair after stroke in hyperlipidemic *** this study,EA was applied to a rat model of hyperlipidemia and middle cerebral artery thrombosis and the condition of neurons and astrocytes after hippocampal injury was *** for the normal group,rats in other groups were fed a high-fat diet throughout the whole *** models were established in rats fed a high-fat diet for 6 *** cerebral artery thrombus models were induced by pasting 50%FeCl3 filter paper on the left middle cerebral artery for 20 minutes on day 50 as the model ***1 group rats received EA at bilateral ST40(Fenglong)for 7 days before the *** in the EA1 and EA2 groups received EA at GV20(Baihui)and bilateral ST40 for 14 days after model *** health was assessed by hematoxylin-eosin staining in the *** was assessed by biochemical methods that measured total cholesterol,triglyceride,low-density lipoprotein and high-density lipoprotein in blood *** analysis was used to confirm the establishment of the *** methods were used to detect the expression of glial fibrillary acidic protein and nerve growth factor in the hippocampal CA1 *** results demonstrated that,compared with the model group,blood lipid levels significantly decreased,glial fibrillary acidic protein immunoreactivity was significantly weakened and nerve growth factor immunoreactivity was significantly enhanced in the EA1 and EA2 *** repair effect was superior in the EA1 group than in the EA2 *** findings confirm that EA can reduce blood lipid,inhibit glial fibrillary acidic protein expression and promote nerve growth factor