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Isolation and Characterization of Mercury Resistant Trichoderma Strains from Soil with High Levels of Mercury and Its Effects on Arabidopsis thaliana Mercury Uptake

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Advances in Microbiology 2018年第7期8卷 600-613页

作者： JoséLuis Hernández-Flores JoséGerardo Bernal Melo Andrés Cruz Hernández Miguel A.Ramos López carlos Saldana Gutiérrez Sergio Romero Gomez Victor Pérez Moreno Rosa P.Calvillo Medina JoséHumberto Valenzuela Soto Sergio Pacheco Hernández George H.Jones Sergio Casas Flores juan Campos-Guillen Laboratorio de Bioseguridad y Análisis de Riesgo Departamento de Ingeniería Genética de PlantasCentro de Investigación y de Estudios Avanzados del IPNIrapuatoGto.México Facultad de Química Universidad Autónoma de QuerétaroCerro de las Campanas s/nQuerétaroQro.México Escuela de Agronomía Universidad De La Salle Bajíocampus Campestre.LeónGto.México Facultad de Ciencias Naturales Universidad Autónoma de QuerétaroAv.De las Ciencias S/NQuerétaroQro.México Centro de Investigación en Química Aplicada Blvd.Enrique Reyna Hermosillo No.140SaltilloCoahuilaMéxico Department of Biology Emory UniversityAtlantaGeorgiaUSA IPICYT División de Biología Molecular.Camino a la presa San JoséNo.2055Colonia Lomas 4a sección.C.P.San Luis PotosíMexico

Traditional mining activities are usually correlated with high levels of soil pollution, which is a major environmental concern. Extensive mining activities have taken place in the San Joaquin region in the State of Querétaro, México resulting in high levels of mercury soil pollution (up to 1532 ± 300 mg/kg). We isolated mercury-resistant fungal strains from the San Joaquin region soils and identified them through morphologic characteristics and ITS rDNA region sequence analysis. We determined that fungi isolated belong to the genus Trichoderma. All the isolates selected showed the ability to catalyze the volatilization of Hg. For air sampling, an active sampling device was constructed and using acid KMnO4 as an absorbent, the concentration of mercury in solution was determined through the cold vapor atomic absorption method. The results show mercury volatilization from the fungal species assay, with a maximum of 213.04 ± 32.6 μg/m3 while mycelium accumulation ranged from less than 17.5 ± 2.9 to 20.0 ± 3.4 μg/g. The fungal isolates were also evaluated for their ability to reduce mercury uptake in Arabidopsis thaliana. These observations suggest the utility of Trichoderma for the mobilization of mercury in those contaminated soils.

关键词： Volatilization Mercury Trichoderma Soil Bioremediation

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Artificial intelligence for renal cancer:From imaging to histology and beyond

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Asian Journal of Urology 2022年第3期9卷 243-252页

作者： Karl-Friedrich Kowalewski Luisa Egen Chanel E.Fischetti Stefano Puliatti Gomez Rivas juan Mark Taratkin Rivero Belenchon Ines Marie Angela Sidoti Abate Julia Muhlbauer Frederik Wessels Enrico Checcucci Giovanni Cacciamani Department of Urology and Urological Surgery University Medical Centre MannheimMannheimGermany Department of Emergency Medicine Brigham and Women’s HospitalHarvard Medical SchoolBostonMAUSA Urology Department University of Modena&Reggio EmiliaModenaItaly ORSI Academy MelleBelgium Department of Urology Hospital Clinico San CarlosMadridSpain Institute for Urology and Reproductive Health Sechenov UniversityMoscowRussia Urology and Nephrology Department Virgen del Rocı´o University HospitalManuel Siurot s/nSevilleSpain Department of Surgery Candiolo Cancer InstituteFPO-IRCCSCandioloTurinItaly USC Institute of Urology University of Southern CaliforniaLos AngelesCAUSA

Artificial intelligence(AI)has made considerable progress within the last decade and is the subject of contemporary *** trend is driven by improved computational abilities and increasing amounts of complex data that allow for new approaches in analysis and *** cell carcinoma(RCC)has a rising incidence since most tumors are now detected at an earlier stage due to improved *** creates considerable challenges as approximately 10%e17%of kidney tumors are designated as benign in histopathological evaluation;however,certain co-morbid populations(the obese and elderly)have an increased peri-interventional *** offers an alternative solution by helping to optimize precision and guidance for diagnostic and therapeutic *** narrative review introduced basic principles and provide a comprehensive overview of current AI techniques for ***,AI applications can be found in any aspect of RCC management including diagnostics,perioperative care,pathology,and *** commonly applied models include neural networks,random forest,support vector machines,and ***,for implementation in daily practice,health care providers need to develop a basic understanding and establish interdisciplinary collaborations in order to standardize datasets,define meaningful endpoints,and unify interpretation.

关键词： Kidney cancer Imaging Technology Artificial intelligence Machine learning

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SYBR GreenⅠ染料法定量PCR检测疟原虫感染及

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分子诊断与治疗杂志 2013年第4期5卷 227-231页

作者：陈江涛刘配芬钟德善潘雪峰杨辉杨立业陆志为杨惠钿林敏 Santiago-m Monte-Nguba juan carlos Salas Ehapo Urbano Monsuy Eyi 广东省惠州市中心人民医院检验中心广东惠州516001 中国第25批援赤道几内亚医疗队广东广州510000 广东省惠州市中医医院广东惠州516001 南方医科大学附属潮州市中心医院中心实验室广东潮州521000 潮州市公安局法医鉴定中心广东潮州521000 Hospital Regional De Malabo

目的评价一种可以快速定量检测疟原虫及虫种鉴别的荧光定量PCR法。方法将SYBR Green Ⅰ法与TaqMan探针法检测疟原虫进行比对,评价定量的准确性及方法的敏感度。以PCR-DNA测序法为金标准,评价SYBR Green Ⅰ法分型的准确性。结果两个方法... 详细信息

目的评价一种可以快速定量检测疟原虫及虫种鉴别的荧光定量PCR法。方法将SYBR Green Ⅰ法与TaqMan探针法检测疟原虫进行比对,评价定量的准确性及方法的敏感度。以PCR-DNA测序法为金标准,评价SYBR Green Ⅰ法分型的准确性。结果两个方法的定量拷贝检测数无显著性差异(P>0.05)。SYBR Green Ⅰ法检测疟原虫的最低检测下限为1×102copy/mL至5×102copy/mL之间。与PCR-DNA测序法比较,SYBR Green Ⅰ法能准确的对感染的疟原虫虫种做准确的鉴定。结论 SYBR Green I染料法定量可同时进行人体疟原虫的定量检测和虫种鉴别,且成本低廉,操作简便快速,可以在疟疾的高发地区推广使用。

关键词：疟疾荧光定量PCR SYBR Green Ⅰ 虫种鉴别

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Cesarean Scar Pregnancy with Iniencephaly and Progression to Placenta Accreta Due to Early Management Rejection

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Maternal-Fetal Medicine 2020年第1期2卷 56-58页

作者： Albaro José Nieto-Calvache María Camila López-Girón Jaime López-Tenorio juan carlos Quintero-Mejía María Andrea Zambrano-Regalado Harry Pachajoa-Londoño María Fernanda Escobar-Vidarte Abnormally Invasive Placenta Clinic Fundación Valle del LiliCali 760032Colombia Clinical postgraduate department Universidad IcesiCali 760032Colombia Clinical Research Center Fundación Valle del LiliCali 760032Colombia Department of Ginecology and Obstetrics Centro médico ImbanacoCali 760032Colombia Department of Genetics Fundación Valle del LiliCali 760032Colombia

To editor:Cesarean scar pregnancy(CSP)is a rare pathology,with an increasingly clear association with morbidly adherent placenta(MAP).1 Although the most recommended treatment is pregnancy termination by cesarean section and scar resection shortly after diagnosis.2 The final decision regarding management depends on the patient's choice,medical advice,the fertility desire,and the social,religious and emotional background that should also be taken into account.

关键词： Placenta accreta Cesarean scar pregnancy Morbidity adherent placenta Pregnancy termination Ultrasonography Prenatal

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Presenilin mutations and their impact on neuronal differentiation in Alzheimer’s disease

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Neural Regeneration Research 2022年第1期17卷 31-37页

作者： Mercedes A.Hernández-Sapiéns Edwin E.Reza-Zaldívar Ana L.Márquez-Aguirre Ulises Gómez-Pinedo Jorge Matias-Guiu Ricardo R.Cevallos juan C.Mateos-Díaz Víctor J.Sánchez-González Alejandro A.Canales-Aguirre Unidad de Evaluación Preclínica Unidad de Biotecnología Médica y FarmacéuticaCentro de Investigación y Asistencia en Tecnología y Diseño del Estado de JaliscoGuadalajaraMéxico Instituto de Neurociencias IdISSCHospital Clínico San CarlosMadridEspaña Biochemistry and Molecular Genetics Department University of AlabamaBirminghamAlabama Unidad de Biotecnología Industrial Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de JaliscoGuadalajaraMéxico Centro Universitario de los Altos Universidad de GuadalajaraGuadalajaraMéxico

The presenilin genes(PSEN1 and PSEN2)are mainly responsible for causing early-onset familial Alzheimer’s disease,harboring~300 causative mutations,and representing~90%of all mutations associated with a very aggressive disease *** 1 is the catalytic core of theγ-secretase complex that conducts the intramembranous proteolytic excision of multiple transmembrane proteins like the amyloid precursor protein,Notch-1,N-and E-cadherin,LRP,Syndecan,Delta,Jagged,CD44,ErbB4,and *** 1 plays an essential role in neural progenitor maintenance,neurogenesis,neurite outgrowth,synaptic function,neuronal function,myelination,and ***,an imbalance caused by mutations in presenilin 1/γ-secretase might cause aberrant signaling,synaptic dysfunction,memory impairment,and increased Aβ42/Aβ40 ratio,contributing to neurodegeneration during the initial stages of Alzheimer’s disease *** review focuses on the neuronal differentiation dysregulation mediated by PSEN1 mutations in Alzheimer’s ***,we emphasize the importance of Alzheimer’s disease-induced pluripotent stem cells models in analyzing PSEN1 mutations implication over the early stages of the Alzheimer’s disease pathogenesis throughout neuronal differentiation impairment.

关键词： familial Alzheimer’s disease familial Alzheimer’s disease-induced pluripotent stem cells models induced pluripotent stem cells neurogenesis neuronal differentiation Notch presenilin 1 PSEN1 mutations γ-secretase complex

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Application of Design of Experiments and Simulation to Improve the Deep Drawing Process of Sheet Metal Forming

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材料科学与工程（中英文B版） 2012年第12期2卷 626-633页

作者： juan carlos Cisneros Ortega Femando Torres Leza Emilio Royo Vazquez Ma Jose Oliveros Colay Aurelio Horacio Heredia Jimenez Department of Engineerings Universidad Popular Aut6noma del Estado de Puebla Puebla 72410 Mdxico Department of Design and Manufacturing Engineering University of Zaragoza Zaragoza 50018 Spain

The objective of this work was the planning, simulation and evaluation of the behavior and interactions of diverse factors in the deep drawing process, in order to prevent defects on formed metal sheet parts. The material used is austenitic stainless steel of 0.4 mm of thickness. Due to the potential high number of experiments, a preliminary selection of cylindrical and hemispherical cup drawing geometries was carried out, as well as a parametric analysis of parts and tools. The influence of the punch and die transition radii, as well as the force of the binder, on the maximum thinning suffered by the material, was studied. The tests were planned by means of CAD/CAE and the design of experiments method. After running the simulation with AutoForm, they were evaluated with FLD, Regression, ANOVA, Six Sigma and Pareto analysis. This sequence allowed us to analyze the influence and sensitivity of several tool and process design parameters and to optimize the process. The results of this investigation have also proven to be useful to simplify and accelerate the design of deep drawing tools.

关键词：金属板材成形模拟设计成形过程实验深拉伸工艺应用拉深奥氏体不锈钢

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The Administration of Ocoxin Increases the Quality of Life of Patients with Advanced Epithelial or Metastatic Ovarian Cancer Undergoing Neoadjuvant Chemotherapy

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Journal of Cancer Therapy 2022年第9期13卷 561-584页

作者： Karen López Miguel Daniel Ricardo Martínez Ávila Lliset Álvarez Pérez Gisselle Martínez Rabasa Alejandro Linchenat Lambert Roberto Esperón Noa Lisandra Heredia Manzano Margarita De Quesada Amigó Rafael I. Reyes Velázquez Danay corrales Otero Ramon Ropero Toirac Idelmis Curbelo Heredia Mircea Betancourt Cabeza Tania A. Agrelo Ramos Rosa María Muñoz Reyes juan Jesús Lence Anta Rosalía Fuentes Sosa Karelia Silvera Candó Kirenia Silva Rodríguez Zaida Lastre Sosa Mayte Robaina García Eduardo Sanz David Marquez Department of Medical Oncology INOR La Habana Cuba Department of Gynaeco-Oncology INOR La Habana Cuba Department of Anatomical Pathology INOR La Habana Cuba Department of Imaging INOR La Habana Cuba Clinical Research Section INOR La Habana Cuba Department of Onco-Psychology INOR La Habana Cuba Clinical Laboratory Department INOR La Habana Cuba Clinical Trials Pharmacy Department INOR La Habana Cuba Department of Biostatistics CENCEC La Habana Cuba Scientific Department CATALYSIS Madrid Spain

Background: 70% of ovarian cancer cases are diagnosed at an advanced stage (III or IV) of the disease and, in turn, with a high prevalence of peritoneal carcinosis and ascites, which leads to progressive malnutrition in patients, with the consequent deterioration of their general condition. There is a very important relationship between nutritional status, quality of life, survival, and the ability to tolerate multidisciplinary treatment of peritoneal carcinosis. Methods: A phase II, open-label, single-center, non-randomised clinical trial was conducted that included 36 patients with advanced disease who were administered the nutritional supplement Ocoxin, 30 ml twice a day, beginning one week before chemotherapy (CT) based on carboplatin/paclitaxel, of which they receive three cycles with neoadjuvant intent. Ocoxin treatment was continued during chemotherapy and for three weeks after completion of the last cycle, as well as during any periods for which this treatment was discontinued due to toxicity. The effect of Ocoxin on the quality of life was assessed through the QLQ C30 and QLQ OV28 questionnaires from the start of treatment until the end of the follow-up period. In addition, the Karnofsky Index and nutritional parameters were assessed. Results: There were no significant differences between adverse events versus baseline values, except in leukocytes, lymphocytes, neutrophils, ALT, and AST. There was no deterioration of the QoL scales, except for those related to the effects of chemotherapy and alopecia. Conclusions: Ocoxin as an adjuvant to chemotherapy appears to improve better tolerance to chemotherapy, showed a good safety profile, and improved quality of life. For further information on Ocoxin neoadjuvant therapy benefits, a phase III clinical trial will be needed.

关键词： Chemotherapy Radiotherapy Ovarian Cancer Oncology Quality of Life Ad-juvant Therapy Supportive Care

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赤道几内亚Bioko岛的恶性疟原虫多药抗性基因(pfMDR-1)的研究

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分子诊断与治疗杂志 2014年第3期6卷 152-156页

作者：卢丹洁陈江涛谢东德杨辉詹小芬杨惠钿杨立业陆志为 Santiago-m Monte-Nguba juan carlos Salas Ehapo Urbano Monsuy Eyi 林敏南方医科大学附属潮州市中心医院检验实验中心广东潮州521000 中国第25批援赤道几内亚医疗队广东广州510000 广东省惠州市中心医院检验中心广东惠州516001 中国第26批援赤道几内亚医疗队广东广州510000 潮州市公安局法医鉴定中心广东潮州521000 Malabo Regional Hospital Malabothe Republic of Equatorial Guinea

目的对赤道几内亚Bioko岛上恶性疟原虫多药抗性基因(pfMDR-1)进行分析,为Bioko岛的疟疾防控和治疗提供依据。方法2012年雨季期间采集的恶性疟原虫感染患者样本151份,用巢式PCR技术特异性扩增N86Y、E130K、Y184F、S1034C、N1042D、V1109I... 详细信息

目的对赤道几内亚Bioko岛上恶性疟原虫多药抗性基因(pfMDR-1)进行分析,为Bioko岛的疟疾防控和治疗提供依据。方法2012年雨季期间采集的恶性疟原虫感染患者样本151份,用巢式PCR技术特异性扩增N86Y、E130K、Y184F、S1034C、N1042D、V1109I、D1246Y耐药分子标记的pfMDR-1基因片段,然后进行测序分析。结果虫株中共发现了4种不同的单倍型:YEY/SNVD、NEF/SNVD、YEF/SNVD和NEY/SNVD。91.39%(138/151)的样本发现了耐药性位点突变,包括3.31%(5/151)的86Y,29.80%的184F,和58.29%(88/151)的双重突变86Y/184F。结论结果表明赤道几内亚Bioko岛上的恶性疟原虫株存在较高比例耐药基因突变和耐药复合基因突变,为当地的抗疟疾选用药物提供指导。

关键词：恶性疟原虫多药抗性基因(pfMDR-1) 耐药突变 Bioko岛

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哥伦比亚重大在建地下基础设施工程的技术描述

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非开挖技术 2016年第2期 19-23页

作者： juan carlos Gutierrez 刘恒伟乌效鸣

在南美哥伦比亚地区正在建设一项十分重大的地下基础设施工程，本文描述该工程出现的一些技术特征．．这些工程，其中包括了：“Centro Parrilla”，对麦德林市中心的污水和饮用水管线网络进行修复和更新：“Parques del Rio”，以另一... 详细信息

在南美哥伦比亚地区正在建设一项十分重大的地下基础设施工程，本文描述该工程出现的一些技术特征．．这些工程，其中包括了：“Centro Parrilla”，对麦德林市中心的污水和饮用水管线网络进行修复和更新：“Parques del Rio”，以另一条地下公路取代地表主要干线公路，与“Boston BigDig”同类工程相比较，这项工程涉及的规模较小；麦德林市的“North Interceptor”工程，一项极具挑战性的采用非开挖中微型隧道技术的工程。为了应对这类重大地下基础设施的建设挑战，以上的工程可以为哥伦比亚其他地区和南美其它城市展示一条技术路径。所以，对以上工程的描述将会涉及到有关设计和施工方面的问题．同时，也包括了社会和环境的影响问题：与社会影响相关的重要因素，在这类城区施工阶段之前。本文会进行一些相关考虑。总之，这些工程为我们提供了一些非常有价值的关于地下基础设施建设的经验，同时，施工中所暴露的问题也具有重要意义。

关键词：非开挖地下工程技术描述

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Iatrogenic bile duct injury with loss of confluence

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World Journal of Gastrointestinal Surgery 2015年第10期7卷 254-260页

作者： Miguel-Angel Mercado Mario Vilatoba Alan Contreras Pilar Leal-Leyte Eduardo Cervantes-Alvarez juan-carlos Arriola Bruno-Adonai Gonzalez Surgery Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán PECEM Facultad de MedicinaUniversidad Nacional Autónoma de México

AIM: To describe our experience concerning the surgical treatment of Strasberg E-4(Bismuth Ⅳ) bile duct injuries. METHODS: In an 18-year period, among 603 patients referred to our hospital for surgical treatment of complex bile duct injuries, 53 presented involvement of the hilar confluence classified as Strasberg E4 injuries. Imagenological studies, mainly magnetic resonance imaging showed a loss of confluence. The files of these patients were analyzed and general data were recorded, including type of operation and postoperative outcome with emphasis on postoperative cholangitis, liver function test and quality of life. The mean time of follow-up was of 55.9 ± 52.9 mo(median = 38.5, minimum = 2, maximum = 181.2). All other patients with Strasberg A, B, C, D, E1, E2, E3, or E5 biliary injuries were excluded from this ***: Patients were divided in three groups: G1(n = 21): Construction of neoconfluence + Roux-en-Y hepatojejunostomy. G2(n = 26): Roux-en-Y portoenterostomy. G3(n = 6): Double(right and left) Rouxen-Y hepatojejunostomy. Cholangitis was recorded in two patients in group 1, in 14 patients in group 2, and in one patient in group 3. All of them required transhepatic instrumentation of the anastomosis and six patients needed live ***: Loss of confluence represents a surgicalchallenge. There are several treatment options at different stages. Roux-en-Y bilioenteric anastomosis(neoconfluence, double-barrel anastomosis, portoenterostomy) is the treatment of choice, and when it is technically possible, building of a neoconfluence has better outcomes. When liver cirrhosis is shown, liver transplantation is the best choice.

关键词： Bile duct injury Hepatojejunostomy Biliary repair

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