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Asperuloside Promotes Apoptosis of Cervical Cancer Cells through Endoplasmic Reticulum Stress-Mitochondrial Pathway

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Chinese Journal of Integrative Medicine 2024年第1期30卷 34-41页

作者： QI Zhi-min WANG Xia LIU Xia ZHAO juan Medical Examination Center Hebei Province Hospital of Chinese MedicineShijiazhuang(050011)China Department of Gynaecology and Obstetrics Hebei Province Hospital of Chinese MedicineShijiazhuang(050011)China Experimental Center for Teaching Hebei Medical UniversityShijiazhuang(050017)China

Objective: To investigate the effects of asperuloside on cervical cancer based on endoplasmic reticulum(ER) stress and mitochondrial pathway. Methods: Different doses(12.5–800 μg/mL) of asperuloside were used to treat cervical cancer cell lines Hela and CaSki to calculate the half maximal inhibitory concentration(IC_(50)) of asperuloside. The cell proliferation was analyzed by clone formation assay. Cell apoptosis, intracellular reactive oxygen species(ros) and mitochondrial membrane potential were determined by flow cytometry. The protein expressions of cleaved-caspase-3, Bcl-2, Bax, Cyt-c, cleaved-caspase-4 and glucose-regulated protein 78(GRP78) were analyzed by Western blot. And the inhibitor of ER stress, 4-phenyl butyric acid(4-PBA) was used to treat cervical cancer cells to further verify the role of ER stress in the apoptosis of cervical cancer cells induced by asperuloside. Results: Asperuloside of 325, 650, and 1300 μg/mL significantly inhibited the proliferation and promoted apoptosis of Hela and CaSki cells(Pros levels, reduced mitochondrial membrane potential, significantly reduced Bcl-2 protein expression level, and increased Bax, Cyt-c, GRP78 and cleaved-caspase-4 expressions(P<0.01). In addition, 10 mmol/L 4-PBA treatment significantly promoted cell proliferation and reduced apoptosis(P<0.05), and 650 μg/mL asperuloside could reverse 4-PBA-induced increased cell proliferation, decreased apoptosis and cleaved-caspase-3,-4 and GRP78 protein expressions(P<0.05). Conclusion: Our study revealed the role of asperuloside in cervical cancer, suggesting that asperuloside promotes apoptosis of cervical cancer cells through ER stress-mitochondrial pathway.

关键词： asperuloside Chinese medicine cervical cancer endoplasmic reticulum stress mitochondrial damage apoptosis

Validating the use of ros-scavenging bacteria as probiotics to increase coral resilience to thermal stress

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Journal of Oceanology and Limnology 2024年第4期42卷 1242-1260页

作者： Xiaoyu TANG Qingsong YANG Ying ZHANG Hanzhang WANG juan LING Haiyan SUN Junde DONG Yanying ZHANG CAS Key Laboratory of Tropical Marine Bio-resources and Ecology South China Sea Institute of Oceanology(SCSIO)Guangzhou 510301China Ocean School Yantai UniversityYantai 264005China Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) Guangzhou 511458China Sanya National Marine Ecosystem Research Station and Key Laboratory of Tropical Marine Biotechnology of Hainan Province Sanya Institute of OceanologySCSIOSanya 572000China Institute of Deep-sea Science and Engineering Chinese Academy of SciencesSanya 572000China University of Chinese Academy of Sciences Beijing 100049China

Thermal stress causes the overproduction and toxic accumulation of reactive oxygen species(ros),which seems to be correlated with coral bleaching and,ultimately,*** reduction of ros concentration within the coral holobiont could minimize the effects of thermal stress and support efforts to reduce coral decline *** the current study,we explored the physiological responses of Pocillopora damicornis to ros-scavenging bacteria inoculation as well as the microbiome restructuring that correlates with ***’s resilience to thermal stress after probiotic *** of corals with ros-scavenging bacteria enhanced coral health and reduced ros ***,the enhanced coral thermal resistance promoted by ros-scavenging bacteria was also correlated with an overall coral microbiome *** addition,the complex network relationships between bacteria and Symbiodiniaceae in corals after ros-scavenging bacteria inoculation contributed to corals’resilience to high ***,coral heat tolerance bacterial biomarkers,such as Myxococcota,were enriched in corals with added ros-scavenging ***,our findings validate the selected ros-scavenging bacteria as coral probiotics that could help corals resist thermal stress on a short ***,our data contribute to our understanding of the potential interactions between different members of the coral holobiont and the use of probiotics as tools to aid coral restoration efforts.

关键词： coral beneficial microorganisms for corals(BMCs) Symbiodiniaceae thermal stress coral bleaching

OsEIL1 and OsEIL2,two master regulators of rice ethylene signaling,promote the expression of ros scavenging genes to facilitate coleoptile elongation and seedling emergence from soil

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Plant Communications 2024年第3期5卷 93-105页

作者： Jinzhu Qiao Ruidang Quan juan Wang Yuxiang Li Dinglin Xiao Zihan Zhao Rongfeng Huang Hua Qin Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China National Key Facility of Crop Gene Resources and Genetic Improvement Beijing 100081China

Successful emergence from the soil is a prerequisite for survival of germinating seeds in their natural *** rice,coleoptile elongation facilitates seedling emergence and establishment,and ethylene plays an important role in this ***,the underlying regulatory mechanism remains largely ***,we report that ethylene promotes cell elongation and inhibits cell expansion in rice coleoptiles,result-ing in longer and thinner coleoptiles that facilitate seedlings emergence from the *** analysis showed that genes related to reactive oxygen species(ros)generation are upregulated and genes involved in ros scavenging are downregulated in the coleoptiles of ethylene-signaling *** investiga-tions showed that soil coverage promotes accumulation of ETHYLENE INSENSITIVE 3-LIKE 1(OsEIL1)and OsEIL2 in the upper region of the coleoptile,and both OsEIL1 and OsEIL2 can bind directly to the promoters of the GDP-mannose pyrophosphorylase(VTC1)gene OsVTC1-3 and the peroxidase(PRX)genes OsPRX37,OsPRX81,OsPRX82,and OsPRX88 to activate their *** leads to increased ascorbic acid content,greater peroxidase activity,and decreased ros accumulation in the upper region of the *** of ros accumulation promotes coleoptile growth and seedling emergence from *** deepen our understanding of the roles of ethylene and ros in controlling coleoptile growth,and this information can be used by breeders to produce rice varieties suitable for direct seeding.

关键词： ethylene ros coleoptile growth emergence rate rice

Sequence-activated chemiluminescence probes: programmable illumination for high-contrast in vivo sensing and imaging

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Science China Chemistry 2024年

作者： Yutao Zhang juan Li Weihong Zhu Zhiqian Guo Shanghai Key Laboratory of Functional Materials Chemistry Joint International Research Laboratory of Precision Chemistry and Molecular Engineering Institute of Fine Chemicals School of Chemistry and Molecular Engineering East China University of Science and Technology State Key Laboratory of Bioreactor Engineering Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell MetabolismEast China University of Science & Technology

Sequence-activated chemiluminescence(CL) technique offers a unique approach to detecting specific biomolecules or cellular events with high sensitivity and spatial resolution. The fundamental principle behind sequence-activated CL probes involves tailoring highly-controllable CL signals in response to specific sequences or events of interest, thus enabling high-contrast signals under complex physiological conditions. This sequential activation could be triggered by enzymes, reactive oxygen species(ros), biothiols or changes in the microenvironment. These unique sequence-activated CL probes bestow several advantages, including minimal background, deep-tissue penetration, and the ability of non-invasive or minimally invasive detection. The sequence-activated CL techniques represent a significant advancement in molecular imaging and diagnostics. In this mini-review, we focus on the latest developments in sequential CL probes, addressing the technical challenges and discussing the future trajectory by harnessing CL signaling and molecular recognition.

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D-type neuropeptide decorated AIEgen/RENP hybrid nanoprobes with light-driven ros generation ability for NIR-Ⅱ fluorescence imaging-guided through-skull photodynamic therapy of gliomas

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Aggregate 2024年第1期5卷 185-195页

作者： Xuelu He Yuan Luo Yanying Li Yuanbo Pan Ryan T.K.Kwok Lulu He Xiaolin Duan Pengfei Zhang Aiguo Wu Ben Zhong Tang juan Li Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology Zhejiang International Cooperation Base of Biomedical Materials Technology and ApplicationChinese Academy of Sciences(CAS)Key Laboratory of Magnetic Materials and DevicesNingbo Cixi Institute of Biomedical EngineeringZhejiang Engineering Research Center for Biomedical MaterialsNingbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingboP.R.China Guangdong Key Laboratory of Nanomedicine Shenzhen Engineering Laboratory of Nanomedicine and NanoformulationsCAS-HK Joint Lab for BiomaterialsResearch Laboratory for Biomedical Optics and Molecular ImagingShenzhen Key Laboratory for Molecular ImagingCAS Key Lab for Health InformaticsShenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhenP.R.China Department of Chemistry Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction and Institute for Advanced StudyThe Hong Kong University of Science and Technology(HKUST)KowloonP.R.China School of Science and Engineering Shenzhen Institute of Aggregate Science and TechnologyThe Chinese University of Hong KongShenzhenP.R.China

Glioma is one of the most common malignant tumors of the central nervous system,leading high mortality rates in ***-induced emission(AIE)photosensitizers-based photodynamic therapy(PDT)has emerged as a promising therapeutic strategy for least-invasive treatment of glioma,which involves local irradiation of the tumor using an external near-infrared(NIR)***,most AIE photosensitizers suffered from poorly penetration of the visible light excitation,bad spatiotemporal resolution in deep tissues and low efficient blood-brain barrier(BBB)crossing ability,which greatly limited the clinical practice of AIE photosensitizers for especially deep-seated brain tumor *** this work,we developed a multifunctional NIR-driven theranostic agent through hybrid of AIE photosensitizers TIND with rare-earth doping nanoparticles(RENPs)NaGdF4:Nd/Yb/Tm with up/down dual-mode conversion *** theranostic agent was further decorated with D-type neuropeptide DNPY for crossing BBB and targeting *** the 808-nm light irradiation,the down-conversion NIR-II luminescence could indicate the position glioma and the upconversion NIR-I luminescence could trigger the AIE photosensitizers producing reactive oxygen species to inhibit orthotopic glioma tumor growth in *** results demonstrate that the integration of Dtype neuropeptide,AIE photosensitizers and RENPs could be promising candidates for in vivo NIR-II fluorescence image-guided through-skull PDT treatments of brain tumors.

关键词： aggregation-induced emission D-type neuropeptide glioma photodynamic therapy rare-earth doping nanoparticles

过度训练导致机体健康损害的关键内在机制探讨

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生物化学与生物物理进展 2024年第8期51卷 1750-1770页

作者：钱帅伟寇现娟李春艳武汉体育学院运动医学院武汉430079 武汉体育学院运动医学院运动训练监控湖北省重点实验室武汉430079

过度训练是训练负荷与身体机能不匹配且恢复期安排不合理,引起疲劳连续过度积累且超过机体所能承受的“度”,进而诱发的一系列功能紊乱或病理状态,是训练与恢复、运动与运动能力、应激与应激耐受性之间的一种失衡状态。过度训练可引起... 详细信息

过度训练是训练负荷与身体机能不匹配且恢复期安排不合理,引起疲劳连续过度积累且超过机体所能承受的“度”,进而诱发的一系列功能紊乱或病理状态,是训练与恢复、运动与运动能力、应激与应激耐受性之间的一种失衡状态。过度训练可引起运动表现下降、食欲减退、体重降低、肌肉疲劳损伤与功能障碍、肌肉萎缩、肌糖原耗竭、肝脏/心肌脂肪沉积、葡萄糖耐受力下降、心脏病理性肥大、运动性心律失常、心肌纤维化和认知功能减退等多种显性改变或病理重塑,但其内在机制却不甚明晰。近年来,细胞分子信号调控理论的逐渐丰富与完善,为研究过度训练导致健康损害的内在机制提供了新的解释范式。本文在传统解释机制基础上,基于细胞分子信号调控理论,从氧化应激、线粒体质量控制、炎症反应、内质网应激和细胞凋亡等视角,对过度训练导致机体健康受损的内在机理进行深入解析,以期为运动员及运动参与者进行科学运动训练、提高训练效果、延长运动寿命、保持身心健康提供重要参考依据。

关键词：过度训练健康受损氧化应激线粒体质量控制炎症内质网应激凋亡

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基于MOF材料的涂层应用与机制研究进展

复合材料学报 2024年第3期41卷 1093-1108页

作者：杜娟汪鸿宇石玉超宋海鹏中国民航大学中欧航空工程师学院天津300300 北京机械设备研究所北京100854

金属有机骨架(MOF)作为一种新型多孔晶体材料,因其具有高孔隙率、结构多样、化学结构可控等特点可被作为纳米粒子和载体使用。基于MOF材料的涂层可兼具MOF本身的优点,但基于MOF材料的涂层应用和机制研究的综述性论文不多。本文针对基于... 详细信息

金属有机骨架(MOF)作为一种新型多孔晶体材料,因其具有高孔隙率、结构多样、化学结构可控等特点可被作为纳米粒子和载体使用。基于MOF材料的涂层可兼具MOF本身的优点,但基于MOF材料的涂层应用和机制研究的综述性论文不多。本文针对基于MOF材料的涂层国内外研究现状进行了介绍,重点阐述了基于MOF材料的涂层防/除冰应用(超疏水表面和光滑液体注入多孔表面(SLIPS))、防腐应用(MOF材料作为纳米粒子和载体)和抗菌应用(基于金属离子释放、基于光动力(PDT)和基于光热(PTT)),并归纳出不同涂层的防/除冰机制(降低水的凝固温度和减少冰的黏附)、防腐机制(直接物理阻隔或生成化合物而达到阻隔效果)和抗菌机制(对真核细胞具有弱毒性的金属离子达到抗菌效果、活性氧(ros)在光照射下激活达到抑菌效果和通过吸收外界光产生热量,随温度升高而达到抗菌效果)。并对基于MOF材料的涂层面临的关键挑战、潜在应用和发展前景进行了展望。

关键词：金属有机骨架涂层防/除冰机制防腐机制抗菌机制

Software Project Management Approaches for Global Software Development： A Systematic Mapping Study

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Tsinghua Science and Technology 2018年第6期23卷 690-714页

作者： Manal El Bajta Ali Idri Joaquín Nicolás ros José Luis Fernández-Alemán juan Manuel Carrillo de Gea Félix García Ambrosio Toval Software Project Management Research Team Ecole Nationale SuperieuredInformatique et d'Analyse des Systemes (ENSIAS)Mohammed V University Rabat 713 Morocco Department of Informaticay Sistemas University of Murcia Murcia 30100 Spain ALARCOS Research GroupDepartment of Information Systems and Technologies University of Castilla-La Mancha (UCLM) Ciudad Real13071 Spain

Global Software Development （GSD） is a well established field of software engineering with the benefits of a global environment. Software Project Management （SPM） plays a key role in the success of GSD. As a result, the need has arisen to study and evaluate the downsides of SPM for GSD, to thereby pave the way for the development of new methods, techniques, and tools with which to tackle them. This paper aims to identify and classify research on SPM approaches for GSD that are available in the literature, to identify their current weaknesses and strengths, and to analyze their applications in industry. We performed a Systematic Mapping Study （SMS） based on six classification criteria. Eighty-four papers were selected and analyzed. The results indicate that interest in SPM for GSD has been increasing since 2006. As a class of approaches, the most frequently reported methods （40%） are those used for coordination, planning, and monitoring, along with estimation techniques that can be used to better match a distributed project. SPM for GSD requires further investigation by researchers and practitioners, particularly with respect to cost and time estimations. These findings will help overcome the challenges that must to be considered in future SPM research for GSD, especially regarding collaboration and time-zone differences.

关键词： Software Project Management (SPM) Global Software Development (GSD) SPM approaches Systematic Mapping Study (SMS)

补阳还五汤对脑缺血再灌注大鼠线粒体氧化损伤和PKCε-Nampt通路的影响

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中成药 2024年第1期46卷 79-86页

作者：尹美娟刘真一靳晓飞周晓红高钰赵月眸高维娟河北中医学院河北省心脑血管病中医药防治研究重点实验室河北石家庄050091

目的探讨补阳还五汤对脑缺血再灌注(I/R)大鼠线粒体氧化损伤及PKCε-Nampt通路的影响。方法大鼠随机分为假手术组、模型组、补阳还五汤组(14.3 g/kg)和依达拉奉组(3 mg/kg),除假手术组外均采用MCAO法建立脑I/R损伤模型,术后24 h各组给... 详细信息

目的探讨补阳还五汤对脑缺血再灌注(I/R)大鼠线粒体氧化损伤及PKCε-Nampt通路的影响。方法大鼠随机分为假手术组、模型组、补阳还五汤组(14.3 g/kg)和依达拉奉组(3 mg/kg),除假手术组外均采用MCAO法建立脑I/R损伤模型,术后24 h各组给予相应剂量药物,给药7 d后通过神经功能评分评估神经功能缺损情况,免疫荧光检测神经元标志物MAP-2表达,观察神经元损伤情况,检测ros、MDA水平和SOD活性评价氧化损伤情况,荧光探针JC-1检测线粒体膜电位变化,修饰酶循环法检测NAD^(+)/NADH比值,Western blot法检测PKCε、p-PKCε、Nampt蛋白表达,免疫组化法检测p-PKCε、Nampt蛋白阳性表达。结果与模型组比较,补阳还五汤组脑梗死体积、神经功能评分降低(Pros、MDA水平降低(P<0.05),SOD活性、线粒体膜电位、NAD^(+)/NADH比值升高(P<0.05),p-PKCε、Nampt蛋白表达升高(P<0.05)。结论补阳还五汤可通过激活PKCε-Nampt信号通路、提高线粒体功能来减轻大鼠脑I/R损伤。

关键词：补阳还五汤脑缺血再灌注损伤线粒体 PKCε Nampt