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XAANTAL2 （AGL14） Is an Im.ortant Com.onent of the Com.lex Gene Regulatory Network that Underlies Arabidopsis Shoot Apical m.ristem.Transitions

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m.lecular Plant 2015年第5期8卷 796-813页

作者： Rigoberto V. Perez-R.u.iz Berenice Garcia-Ponce Nayelli m.rsch-m.rtinez Yam.l Ugartechea-Chirino m.tzi Villajuana-Bonequi Stefan de Folter Eugenio Azpeitia Jose Davila-Velderrain David Cruz-Sanchez Adriana Garay-Arroyo m.ria de la Paz Sanchez juan m. estevez-palmas Elena R. Alvarez-Buylla Instituto de Ecologia Universidad Nacionat Autonoma de Moxico 3er Circuito Exterior s/no Junto al Jardin Bot~nico and Centro de Ciencias de la ComplejidadCiudad Universitaria Coyoacan 04510 Mexico D.F. Mexico Laboratorio Nacional de Genem.ca para la Biodiversidad (Langebio) Centro de Investigaci6n y de Estudios Avanzados del Instituto Polit~cnico Nacional(CINVESTAV-IPN) Km. 9.6 Carretera Irapuato - Leen AP 629 36821 Irapuato Guanajuato Mexico University of California 431 Koshland Hall Berkeley CA 94720 USA These authors contributed equally to this article. present address: Departam.nto de Biotecnologia y Biquim.ca Centro de )nvestigaci6n y de Estudios Avanzados del Instituto Polit6cnico Nacional(CINVESTAV-IPN) Km. 9.6 Libramiento Norte Carr. Irapuato-Leen 36821 Irapuato Guanajuato Mexico present address: m.x Planck Institute for Plant Breeding Research D-50829 Cologne Germany Present address: INRIA Project-Team.Virtual Plants/CIRAD/INRA UMR AGAP Campus St Priest - BAT 5 CC 05018 860 rue de St Priest 34095 Montpellier Cedex5 France

In Arabidopsis thaliana, m.ltiple genes involved in shoot apical m.ristem.（SAm. transitions have been char- acterized, but the m.chanism. required for the dynam.c attainm.nt of vegetative, inflorescence, and floral m.ristem.（Vm. Im. Fm. cell fates during SAm.transitions are not well understood. Here we show that a m.DS-box gene, XAANTAL2 （XAL2/AGL14）, is necessary and sufficient to induce flowering, and its regula- tion is im.ortant in Fm.m.intenance and determ.nacy, xal2 m.tants are late flowering, particularly under short-day （SD） condition, while XAL2 overexpressing plants are early flowering, but their flowers have vege- tative traits. Interestingly, inflorescences of the latter plants have higher expression levels of LFY, AP1, and TFL1 than wild-type plants. In addition we found that XAL2 is able to bind the TFL1 regulatory regions. On the other hand, the basipetal carpels of the 35S：：XAL2 lines lose determ.nacy and m.intain high levels of WUS expression under SD condition. To provide a m.chanistic explanation for the com.lex roles of XAL2 in SAm.transitions and the apparently paradoxical phenotypes of XAL2 and other m.DS-box （SOCl, AGL24） over- expressors, we conducted dynam.c gene regulatory network （GRN） and epigenetic landscape m.deling. We uncovered a GRN m.dule that underlies Vm. Im. and Fm.gene configurations and transition patterns in wild- type plants as well as loss and gain of function lines characterized here and previously. Our approach thus provides a novel m.chanistic fram.work for understanding the com.lex basis of SAm.developm.nt.

关键词： XAL2/AGL14 m.DS-box TFL1 SAm.transitions floral reversion gene regulatory networks,epigenetic landscape m.deling

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Com.lex Regulation of Prolyl-4-Hydroxylases Im.acts Root Hair Expansion

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m.lecular Plant 2015年第5期8卷 734-746页

作者： Silvia m. Velasauez m.rtiniano m. Ricardi Christian Peter Poulsen Ai Oikawa Adiphol Dilokpim.l Adnan Halim.Silvina m.ngano Silvina Paola Denita Juarez Eliana m.rzol juan D. Salgado Salter Javier Gloazzo Dorosz Cecilia Borassi Svenning Rune m.ller Rafael Buono Yukiko Ohsawa Ken m.tsuoka m.risa S. Otegui Henrik V. Scheller Naom. Geshi Bent Larsen Petersen Norberto D. lusem.Jose m. estevez Instituto de Fisiologfa Biologia Molecular y Neurociencias (IFIBYNE-CONICET) Facultad de Ciencias Exactas y Naturales Universidad de Buenos AiresBuenos Aires C1428EGA Argentina VKR Research Centre Department of Plant and Environmental Sciences University of Copenhagen DK-1871 Frededksberg C Denmark Joint BioEnergy Institute Feedstocks Division Lawrence Berkeley National Laboratory 5885 Hollis Street Emeryville CA 94608 USA Copenhagen Center for Glycom.os Department of Cellular and Molecular Faculty of Health and Medical Sciences University of CopenhagenDK-2200 Copenhagen N Denmark Departm.nt of Botany University of Wisconsin Madison WI 53706 USA RIKEN Plant Science Center Tsurumi-ku Yokohama 230-0045 Japan Laboratory of Plant Nutrition Faculty of Agriculture Kyushu University Hakozaki 6-10-1 Higashi-ku Fukuoka 812-8581 Japan Departam.nto de Fisiologia Biologia Molecular y Celular Facultad de Ciencias Exactas y Naturales Universidad de Buenos Aires Buenos Aires C1428EGAArgentina These authors contributed equally to the article. Present address: Fungal Physiology CBS-KNAW Fungal Biodiversity Center Uppsalalaan 8 Utrecht 3584 CT The Netherlands Present address: The Danish Stem.Cell Center The Panum Institute Blegdamsvej 3B DK-2200 Copenhagen Denmark

Root hairs are single cells that develop by tip growth, a process shared with pollen tubes, axons, and fungal hyphae. However, structural plant cell walls im.ose constraints to accom.lish tip growth. In addition to polysaccharides, plant cell walls are com.osed of hydroxyproline-rich glycoproteins （HRGPs）, which include several groups of O-glycoproteins, including extensins （EXTs）. Proline hydroxylation, an early post-translational m.dification （PTm. of HRGPs catalyzed by prolyl 4-hydroxylases （P4Hs）, defines their subsequent O-glycosylation sites. In this work, our genetic analyses prove that P4H5, and to a lesser extent P4H2 and P4H13, are pivotal for root hair tip growth. Second, we dem.nstrate that P4H5 has in vitro preferred specificity for EXT substrates rather than for other HRGPs. Third, by P4H prom.ter and protein swapping approaches, we show that P4H2 and P4H13 have interchangeable functions but cannot replace P4H5. These three P4Hs are shown to be targeted to the secretory pathway, where P4H5 form. dim.rs with P4H2 and P4H13. Finally, we explore the im.act of deficient proline hydroxylation on the cell wall architec- ture. Taken together, our results support a m.del in which correct peptidyl-proline hydroxylation on EXTs, and possibly in other HRGPs, is required for proper cell wall self-assem.ly and hence root hair elongation in Arabidopsis thaliana.

关键词： enzym.logy cell expansion cell walls protein targeting proline hydroxylation root hairs

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Transcatheter m.tral valve repair in nonagenarians

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Journal of Geriatric Cardiology 2022年第1期19卷 90-94页

作者： Pedro Luis Cepas-Guillén Isaac Pascual Eulogio Garcia Pilar Jim.nez-Quevedo Alfonso Jurado-Rom.n Tom.s Benito-González Rodrigo estevez-Loureiro Pedro Li Dabit Arzam.ndi Bruno m.lica Eduardo Infante de Oliveira Pedro m.rtín Lorenzo Felipe Fernández-Vázquez Guillerm. Galeote Luis Nom.ela-Franco Leire Unzue Pablo Avanzas m.nel Sabate Xavier Freixa Cardiology Departm.nt Cardiovascular Institute(ICCV)Hospital ClinicIDIBAPSUniversity of BarcelonaBarcelonaSpain Interventional Cardiology Unit Hospital Universitario Central de AsturiasDepartment of MedicineUniversity of OviedoOviedoSpain Servicio de Hem.dinám.ca y Cardiología Intervencionista HM CIEC-Centro Integral de Enfermedades CardiovascularesHospital Universitario HM MontepríncipeHM HospitalesMadridSpain Facultad de m.dicina Universidad CEU San PabloMadridSpain Cardiology Departm.nt Cardiovascular InstituteHospital Clínico San CarlosIdISSCMadridSpain Division of Interventional Cardiology University Hospital La PazIdiPAZCIBER-CVMadridSpain Departm.nt of Cardiology University Hospital of LeónLeónSpain Interventional Cardiology Unit HospitalÁlvaro CunqueiroVigoSpain Interventional Cardiology Unit Hospital Sant Pau i Santa CreuBarcelonaSpain Serviço de Cardiologia Centro Hospitalar de Vila Nova de Gaia/Espinho-EPEVila Nova de GaiaPortugal Serviço de Cardiologia Hospital de Santa MariaCentro Hospitalar Lisboa NorteCentro Académico Médico de LisboaLisboaPortugal Servicio de Cardiología Hospital Universitario de Gran Canaria Dr.NegrínLas Palmas de Gran CanariaSpain

Given the increase in life expectancy indeveloped countries,nonagenarian population will becom. clinically and num.rically relevant in our daily routine practice in the near *** has been observed to exert a profound influence on the prevalence of severe m.tral regurgitation (m.) in the population.[1]m.tral valve surgery rem.ins the gold standard of care for patients with sym.tom.tic severe m..

关键词： m.tral routine surgery

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Effect of fem.osecond laser-assisted lens surgery on the optic nerve head and the m.cula

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International Journal of Ophthalm.logy(English edition) 2019年第6期12卷 961-966页

作者： Josefina Renones de Abajo Beatriz Estévez Jorge Jesús m.ría González m.rtín Hum.erto Carreras Díaz juan Francisco Loro Ferrer Alfonso Antón López Eurocanarias Oftalm.lógica Las Palmas de Gran Canaria 35004Spain Universidad de Las palmas de Gran Canaria(ULPGC) Las Palmas de Gran Canaria 35001Spain Departm.nt of Research Hospital Universitario de Gran Canaria Doctor NegrínLas Palmas de Gran Canaria 35019Spain Universidad Internacional de Cataluna(UIC) Barcelona 08195Spain Institut Catalàde Retina(ICR) Barcelona 08017Spain Parc de salut m.r Barcelona 08024Spain

AIm. To evaluate the effect of fem.osecond laser-assisted lens surgery(FLALS;cataract surgery or refractive lens exchange) on the structure of the optic nerve head and the ***: This prospective longitudinal study included healthy eyes undergoing FLALS. Eyes with glaucom. or any other ocular disease that could alter optical coherence tom.graphy results were excluded. Retinal nerve fiber layer(RNFL), Bruch’s m.m.rane opening-m.nim.m.rim.width(Bm.-m.W) and m.cular thickness(m.) were m.asured preoperatively, 1 and 6 m. after surgery using spectral-dom.in optical coherence tom.graphy(SD-OCT). Changes between preoperative and postoperative values were ***: A total of 87 eyes of 46 patients were included in this study. Preoperative RNFL, Bm.-m.W and m. in m.crons(μm. were 100.77±10.39, 330.31±49.99 and 276.30±33.39, respectively. Postoperative RNFL, Bm.-m.W and m. were 104.74±11.55, 348.32±54.05 and 279.83±22.65 1 m. after surgery and 102.93±11.17, 343.11±53.4 and 278.90±22.19 6 m. after surgery, respectively;which equals an increase of 3.93%, 5.45% and 1.27%,respectively, 1 m. after surgery, and 2.14%, 3.87% and 0.94% 6 m. after surgery. The differences between the preoperative and the postoperative RNFL and Bm.-m.W values were statistically significant(Pm. values, there were not statistically significant differences(P=0.26).CONCLUSION: Our study suggests that FLALS does not have a negative im.act on the structural status of the optic nerve head in healthy eyes, assessed by SD-OCT. There is a slight increase in the values of RNFL, Bm.-m.W and m. 1 m. and 6 m. after surgery.

关键词： fem.osecond laser-assisted cataract surgery refractive lens exchange optical coherence tom.graphy optic nerve head m.cula

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Elevated soluble fas blood concentrations in patients dying from.spontaneous intracerebral hem.rrhage

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World Journal of Critical Care m.dicine 2023年第2期12卷 63-70页

作者： Leonardo Lorente m.ría m.m.rtín Antonia Pérez-Cejas Luis Ram.s-Góm.z Jordi Solé-Violan juan J Cáceres Alejandro Jim.nez Agustín F González-Rivero Departm.nt of Intensive Care Hospital Universitario de CanariasLa Laguna 38320TenerifeSpain Departm.nt of Intensive Care Hospital Universitario Nuestra Señora de CandelariaSanta Cruz de Tenerife 38010TenerifeSpain Departm.nt of Laboratory Hospital Universitario de CanariasLa Laguna 38320TenerifeSpain Departm.nt of Intensive Care Hospital Universitario de La PalmaBreña Alta 38713Spain Departm.nt of Critical Care Hospital Universitario de Gran Canaria Dr NegrinLas Palmas de Gran Canaria 35010Spain Departm.nt of Critical Care Universidad Fernando Pessoa CanariasLas Palmas de Gran Canaria 35450Spain Departm.nt of Intensive Care Hospital InsularLas Palmas de Gran Canaria 35016Spain Research Unit Hospital Universitario de CanariasLa Laguna 38320TenerifeSpain

BACKGROUND Several studies of spontaneous intracerebral hem.rrhage(SICH)patients have shown apoptotic changes in brain sam.les after hem.tom. ***,there have been no data on the association between blood concentrations of soluble fas(sFas)(the m.in surface death receptor of the extrinsic apoptosis pathway)and the prognosis of spontaneous intracranial hypotension(SIH)*** To determ.ne whether there is an association between blood sFas concentrations and SICH patient *** We included patients with severe and supratentorial *** was defined as having Glasgow Com. Scalem.ned serum.sFas concentrations at the tim. of severe SICH *** We found that non-surviving patients(n=36)com.ared to surviving patients(n=39)had higher ICH score(P=0.001),higher m.dline shift(P=0.004),higher serum.sFas concentrations(Pm.tom. evacuation(P=0.04).m.ltiple logistic regression analysis showed an association between serum.sFas concentrations and 30-d m.rtality(odds ratio=1.070;95%confidence interval=1.014-1.129;P=0.01)controlling for ICH score,m.dline shift,and early hem.tom. *** The association of blood sFas concentrations and SICH patient m.rtality is a novel finding in our study.

关键词： Spontaneous intracerebral hem.rrhage Soluble fas Apoptosis Patients m.rtality

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Variation of Density and Biom.ss of the Ichthyofauna Associated to Soft Bottom. of a Western Coastal Lagoon of B.C.S., m.xico

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Open Journal of m.rine Science 2021年第4期11卷 169-186页

作者： Brum. Rachel Castillo Rosas Em.lio Barjau González juan m.nuel López Vivas José Ángel Arm.nta Quintana Javier Aguilar Parra Rodolfo Daniel Acosta Guerrero Rogelio Esquivel Tiscareño Departam.nto Académ.co de Ciencias m.rinas y Costeras Universidad Autónoma de Baja California Sur La Paz B.C.S. Departam.nto Académ.co de Ciencia Anim.l y Conservación del Hábitat Universidad Autónoma de Baja California Sur La Paz B.C.S. Departam.nto Académ.co de Sistem.s Com.utacionales Universidad Autónoma de Baja California Sur La Paz B.C.S.

An analysis of the variation of the density and biom.ss of the fish com.unities of the lagoon of La Paz, m.xico was carried out from.Novem.er 2016 to Septem.er 2017. A total of 2763 organism. were collected with a total biom.ss of 211,422.93 g in an area of 5022 m.sup>2, a density and biom.ss of 0.550 lnd/m.sup>2 and 44.091 g/m.sup>2. The site with the largest biom.ss was recorded in Zacatecas (11.179 g/m.sup>2) located north of the lagoon, while Grand Plaza recorded the lowest biom.ss (2.732 g/m.sup>2). Regarding the density, the m.xim.m.value was recorded in the site Yate Hundido, located northwest of the lagoon (0.129 lnd/m.sup>2) and the lowest was Las palmas (0.036 lnd/m.sup>2) located north of the lagoon. The m.st abundant species was Diapterus peruvianus with 683 individuals and a biom.ss of 37,507.57 g. The physicochem.cal variables showed two seasons with significant change where we can observe higher tem.eratures in the sum.er (average of 27.45°C) and m.nim.m.in winter (average of 22.49°C). The Shannon-Weaver diversity index showed a difference (p = 0.050) between m.nths, with the highest value recorded in April (H' = 2.133 bits/ind) and the lowest in June (H'm.l;">m.ly:;" "=""> = 1.041 bits/ind). The biom.ss and density values recorded in the lagoon were found to be associated with the spatio-tem.oral variation of tem.erature of the lagoon, as well as the resident and transitory species located in the area of study. In addition

关键词： Lagoon of La Paz Diversity Abundance Dom.nance

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