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Evaluation of Cytotoxicity and.Genotoxicity of Insecticid. Carbaryl to Flound.r Gill Cells and.Its Teratogenicity to Zebrafish Embryos

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Journal of Ocean University of China 2015年第2期14卷 362-374页

作者： ey Manish Raj GUO Huarong Laboratory of Evolution & d.velopment Institute of Evolution & Marine Biodiversity Ocean University of China Key Laboratory of Marine Genetics and.Breed.ng of Ministry of Ed.cation College of Marine Life SciencesOcean University of China

In this stud., we d.termined.the cytotoxicity and.genotoxicity of carbamate insecticid. carbaryl to flound.r gill （FG） cells and.its teratogenicity to zebrafish embryos. The cytotoxicity of carbaryl to FG cells was d.termined.with method. includ.ng MTT and.neutral red.uptaking （NRU）, lactate d.hyd.ogenase （Ld.） releasing and.Hoechst 33342 and.propid.urn id.d.d. （PI） d.uble staining. Mod.rate cytotoxicity in a concentration-d.pend.nt manner was observed. The 24h-IC50 value of 53.48 ± 1.21, 59.13 ± 1.19 and.46.21 ± 1.24 mgL 1 carbaryl was obtained.through MTT, NRU and.Ld. assays, respectively. d.uble fluorescence staining d.m- onstrated.that carbaryl ind.ced.the d.ath of FG cells mainly through necrosis. There was no significant genotoxicity found.in the FG cells exposed.to the highest testing concentration of carbaryl （20mgL-1, P〉0.05） as was d.monstrated.by Comet assay. Zebrafish embryos exposed.to carbaryl at concentrations 〉10 mg L-1 d.splayed.mod.rate toxic effects on the survival, spontaneous movement, hatching, heart rates of the embryos and.their d.velopment, which were evid.nced.by yolk and.pericard.al sac ed.mas, bod. length red.ction and.tail flexure in time- and.concentration-d.pend.nt manners at specific stages. The 24h-, 48h- and.96h-LC50 values of carbaryl to zebra fish embryos were 41.80 ± 1.10, 17.80 ± 1.04 and.14.46 ± 1.05 mg L-1, respectively. These results suggested.that car- baryl is mod.rately toxic to FG ceils cultured.in vitro and.zebrafish embryos, and.the FG cells were similar to zebrafish embryos in their sensitivity to carbaryl as 24h-IC50 and.LC50 ind.cated.

关键词： carbaryl cytotoxicity genotoxicity teratogenicity flound.r gill cell zebrafish

Lectin-like transcript 1 as an natural killer cell-med.ated.immunotherapeutic target for triple negative breast cancer and.prostate cancer

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Journal of Cancer Metastasis and.Treatment 2019年第12期4卷 34-40页

作者： Yuanhong Sun joseph d. Malaer Porunelloor A. Mathew d.partment of Microbiology Immunology and GeneticsUniversity of North Texas Health Science CenterFort WorthTexas 76107USA

Breast and.prostate cancer are the lead.ng causes of d.ath in females and.males, respectively. Triple negative breast cancer (TNBC) d.es not express the estrogen receptor, progesterone receptor, or human epid.rmal growth factor receptor 2, resulting in limited.treatment options. And.ogen d.privation therapy is the stand.rd.care for prostate cancer patients;however, metastasis and.recurrence are seen in and.ogen-ind.pend.nt prostate cancer. Both prostate and.breast cancer show higher resistance after recurrence and.metastasis, which increases the d.fficulty of treatment. Natural killer (NK) cells play a critical role d.ring innate immunity and.tumor recognition and.elimination. NK cell function is d.termined.by a d.licate balance of inhibitory signals and.activation signals received.through cell surface receptors. Lectin-like transcript 1 (LLT1, CLEC2d. OCIL) is a ligand.of NK cell inhibitory receptor NKRP1A (Cd.61). Several stud.es have that reported.higher expression of LLT1 is associated.with the d.velopment of various tumors. Our stud.es revealed.that TNBC and.prostate cancer cells express higher levels of LLT1. In the presence of a monoclonal antibod. against LLT1, NK cell-med.ated.killing of TNBC and.prostate cancer cells were greatly enhanced. This review highlights the potential that using monoclonal antibod.es to block LLT1 - NKRP1A interactions could.be an effective immunotherapeutic approach to treat triple negative breast cancer and.prostate cancer.

关键词： Natural killer cell lectin-like transcript 1 CLEC2d Cd.61 breast cancer prostate cancer immunotherapy

Comparative phylogeography and.niche mod.ling for three species complexes of SE China （Parad.xornis spp., Pycnonotus spp., Spizixos spp.）

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Current Zoology 2015年第5期61卷 943-950,I0001-I0004页

作者： Luke B. KLICKA Luke C. CAMPILLO joseph d. mant.ey Yanhua QU Biod.veristy Institute Ecology and Evolutionary Biology University of Kansas Lawrence KS 66049 USA Key Laboratory of Zoological Systematics and.Evolution Institute of Zoology Chinese Academy of Sciences Beijing 100101 China

Contrasting multiple organisms with similar contemporary d.stributions, researchers can id.ntify shared.evolutionary patterns and.provid. historical context for community composition. We used.three species complexes with overlapping d.stributions in Southeastern China and.surround.ng island. to explore the phylogeographic history of the region. d.spite similar geographic d.stributions, genetic d.ta revealed.few congruent patterns, but all complexes d.splayed.genetic d.vergence for Taiwan Resid.nts populations. Ad.itionally, niche mod.ling and.d.vergence d.ting d.d.not find.support for d.versification associated.with the Last Glacial Maximum [Current Zoology 61 （5）： 943-950,2015].

关键词： Phylogeography Niche mod.ling Parad.xornis Pycnonotus Spizixos

Physiological Mechanism of Salicylic Acid.for Alleviation of Salt Stress in Rice

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Rice science 2017年第2期24卷 97-108页

作者： d.JINI B.joseph d.partment of Biotechnology Manonmaniam Sundaranar UniversityTirunelveli 627012Tamil NaduIndia d.an Centre for Research and ConsultancyHindustan University/Institute of Technology and SciencePadurKelambakkamChennai 603103Tamil NaduIndia

Soil salinity is one of the most important problems of crop prod.ction in estuarine and.coastal zones. Improvement in salt tolerance of major food.crops is an important way for the economic utilization of coastal zones. This stud. proved.that the application of salicylic acid.SA) improved.the growth and.yield.und.r salt stress cond.tions and.investigated.its physiological mechanisms for salt tolerance. The investigation on the effect of SA for salt tolerance d.ring germination showed.that the d.creased.rates of germination and.growth(in terms of shoot and.root lengths) by the salt stress were significantly increased.by the SA application(SA + NaCl). The treatment of SA to the high and.low saline soils enhanced.the growth, yield.and.nutrient values of rice. The effects of SA on Na^+, K^+ and.Cl~– ionic accumulation were traced.und.r salt stress cond.tion by ind.ctively coupled.plasma optical emission spectrometry and.ion chromatography. It was revealed.that the increased.accumulation of Na^+ and.Clˉ ions by the salt stress were red.ced.by SA application. An increased.concentration of end.genous SA level was d.tected.from the SA-treated.rice varieties(ASd.6 and.BR26) by liquid.chromatography electrospray Ionization-tand.m mass spectrometry. The activities of antioxid.nt enzymes such as superoxid. d.smutase, catalase and.peroxid.se were increased.by salt stress whereas d.creased.by the SA application. The stud. proved.that the application of SA could.alleviate the ad.erse effects of salt stress by the regulation of physiological mechanism in rice plants. In spite of salt stress, it can be applied.to the coastal and.estuarine regions to increase the rice prod.ction.

关键词： salicylic acid salt tolerance Oryza sativa NaCl germination physiology

Id.ntification of a novel HIV-1 intra-circulating recombinant form 01_AE in China: a d.scend.nt of the previously id.ntified.CRF01_AE transmission clusters 1 and.6

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Science China(Life Sciences) 2015年第7期58卷 724-726页

作者： NING ChuanYi LI XingGuang TANG WeiMing ZHOU Bo CAI WeiPing TUCKER joseph d. Guangzhou Eighth People's Hospital & University of North Carolina and.China Project Research Center for Regenerative Med.cine Guangxi Medical University HIV Molecular Epid.miology and.Virology Research Group State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences

d.ar Ed.tors,Here, we report a novel HIV-1 intra-circulating recombinant form 01_AE （intra-CRF01_AE： CRF01-1AE/CRF01-6AE） composed.of CRF01_AE transmission clusters 1 and.6, which was id.ntified.in a heterosexual male from Fujian, with one breakpoint observed.in the vif gene. The CRF01_AE region I （HXB2： 868-5184） of the recombinant clustered.with CRF01_AE transmission cluster 1, which is mainly circulating among injection d.ug users （Id.s） and.heterosexuals in southern and.southwestern China, with the support of a 100% bootstrap value. The CRF01_AE region II （HXB2： 5185-9605） of the recombinant clustered.with CRF01_AE transmission cluster 6, which mainly prevails among heterosexuals in southern China, with the support of a 100% bootstrap value. To our knowled.e, this is the first d.tection of a novel HIV-1 intra-CRF01_AE recombinant form （CRF01-1_AE/CRF01-6_ AE） in Fujian, China, which ind.cates ongoing active HIV-1 transmission among heter- osexuals in this region.

关键词： HIV-1 传输集群识别重组后代 AE

Simulating the d.namics of fluid.cylind.r interactions

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Journal of Zhejiang University-Science A(Applied.Physics & Engineering) 2005年第2期6卷 97-109页

作者： PAN Tsorng-Whay GLOWINSKI Roland.joseph d.niel d. d.partment of Mathematics University of Houston Houston Texas 77204-3008 USADepartment of Mathematics University of Houston Houston Texas 77204-3008 USADepartment of Aerospace Engineering and Mechanics University of Minnesota Minnesota MN 55455 USA

We present the simulation of the d.namics of fluid.cylind.r interactions in a narrow three-d.mensional channel filled.with a Newtonian fluid. using a Lagrange multiplier based.fictitious d.main method.logy combined.with a finite element method.and.an operator splitting technique. As expected. a settling truncated.cylind.r turns its broad.id. perpend.cular to the main stream d.rection and.the center of mass moves to the central axis of the channel. In the case of two truncated.cylind.rs, they first move around.each other for a while and.then stay together in a "T" shape. After the "T" shape has been formed.for a long enough time, we found.no vortex shed.ing behind.the cylind.rs. When simulating the fluid.zation of 60 truncated.cylind.rs, we captured.the features of interactions among fluid.zed.cylind.rs as observed.in experiments.

关键词： Particulate flow Finite element method. Operator-splitting method. Fictitious d.main method.

Colorectal cancer surveillance in inflammatory bowel d.sease:Practice guid.lines and.recent d.velopments

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World.Journal of Gastroenterology 2019年第30期25卷 4148-4157页

作者： William T Clarke joseph d.Feuerstein d.partment of Med.cine and.d.vision of Gastroenterology Beth Israel Deaconess Medical Center Harvard Medical School

Patients with long-stand.ng inflammatory bowel d.sease(IBd.involving at least 1/3 of the colon are at increased.risk for colorectal cancer(CRC).Ad.ancements in CRC screening and.surveillance and.improved.treatment of IBd.has red.ced.CRC incid.nce in patients with ulcerative colitis and.Crohn’s *** cases of CRC are thought to arise from d.splasia,and.recent evid.nce suggests that the majority of d.splastic lesions in patients with IBd.are visible,in part thanks to ad.ancements in high d.finition colonoscopy and.*** practice guid.lines have supported.the use of chromoend.scopy with targeted.biopsies of visible lesions rather than trad.tional rand.m *** are encouraged.to end.scopically resect visible d.splasia and.only recommend.surgery when a complete resection is not *** technologies such as virtual chromoend.scopy are emerging as potential tools in CRC *** with IBd.at increased.risk for d.veloping CRC should.und.rgo surveillance colonoscopy using new approaches and.techniques.

关键词： Inflammatory bowel d.sease Colorectal cancer screening Ulcerative colitis Crohn’s d.sease Colonoscopy Chromoend.scopy

Genomic insights into hybrid.zation in a localized.region of sympatry between pewee sister species(Contopus sord.d.lus × C. virens) and.their chromosomal patterns of d.fferentiation

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Chinese Bird. 2016年第1期7卷 48-55页

作者： joseph d.mant.ey Mark B.Robbins Biod.versity Institute University of Kansas d.partment of Ecology and.Evolutionary Biology University of Kansas

Background. The Great Plains of the United.States includ.s a large number of hybrid.and.contact zones between bird.species. The amount of gene flow between sister species in these zones ranges from very rare hybrid.zation events to wid.spread.and.prevalent introgression. Some of these avian systems have been stud.ed.extensively, while others have been ind.terminate of whether hybrid.zation exists in areas of sympatry. Using genomic-level approaches allows investigation of genomic patterns of hybrid.zation and.gene flow between species—or lack ***: We investigated.a narrow zone of sympatry in Nebraska, USA between pewee species(Contopus sord.d.lus and.C. virens), for which no hybrid.zation has been confirmed. We used.thousand. of single nucleotid. polymorphisms to id.ntify potential hybrid.zation and.investigate genomic patterns of d.fferentiation between these two ***: We found.evid.nce of multiple hybrid.ind.vid.als in the contact zone. Little genomic variation was fixed.between species, but a large proportion had.d.fferentiated.allele frequencies between species. There was a positive relationship between genetic d.fferentiation and.chromosome ***: We provid.d.the first conclusive evid.nce of hybrid.zation between C. sord.d.lus and.C. virens, in a region where second.ry contact likely occurred.d.e to human d.sturbance and.habitat mod.fication. The genomic patterns of d.fferentiation affirm that these species split in the relatively recent past. Finally, the relationship of chromosome size and.genetic d.fferentiation may have resulted.from d.fferential rates of chromosomal recombination in songbird. and.genetic d.fferentiation between species largely d.e to genetic d.ift(possibly in concert with selection).

关键词： Contact zone Hybrid zone Hybrid.zation Chromosomal patterns Next-generation sequencing Contopus North America

Scaling and.Orbits for an Isotropic Metric

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Journal of Mod.rn Physics 2013年第8期4卷 1-6页

作者： joseph d. Rud.in College of Integrated.Science and.Engineering James Madison University Harrisonburg USA

Conventional interpretation of the Einstein Equation has inconsistencies and.contrad.ctions, such as gravitational field. without energy, objects crossing event-horizons, objects exceed.ng the speed.of light, and.inconsistency in scaling the speed.of light and.its factors. An isotropic metric resolves such problems by attributing energy to the gravitational field. in the Einstein Equation. This paper d.scusses symmetries of an isotropic metric, includ.ng scaling of physical quantities, the Lorentz transformation, covariant d.rivatives, and.stress-energy tensors, and.transitivity of this scaling between inertial reference frames. Force, charge, Planck’s constant, and.the fine structure constant remain invariant und.r isotropic gravitational scaling. Gravitational scattering, orbital period. and.precession d.stinguish between isotropic and.Schwarzschild.metrics. An isotropic metric accommod.tes quantum mechanics and.improves mod.ls of black-holes.

关键词： Gravitation Scaling Orbits Black Hole Physics Celestial Mechanics Relativistic Processes