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Robust transcriptional indicators of immune cell death revealed by spatiotemporal transcriptome analyses

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Molecular Plant 2022年第6期15卷 1059-1075页

作者： jose Salguero-Linares Irene Serrano Nerea ruiz-Solani Marta Salas-Gómez Ujjal Jyoti Phukan Victor Manuel González MartíBernardo-Faura Marc Valls David Rengel Nuria S.Coll Centre for Research in Agricultural Genomics(CRAG) CSIC-IRTA-UAB-UBCampus UABBellaterra08193 BarcelonaSpain LIPM Universitéde ToulouseINRACNRS84195 Castanet-TolosanFrance INRAE GeT-PlaGeGenotoul31326 Castanet-TolosanFrance Department of Genetics Universitat de Barcelona08028 BarcelonaSpain Consejo Superior de Investigaciones Científicas(CSIC) BarcelonaSpain Department of Plant Molecular Biology and Physiology.Albrechtvon Haller Institute for Plant Sciences niversity of GottingenJulia-Lermontowa-Weg3.37077 GottingenGrmany Institut de Phammacologie et de Biologie Structurale IPBS Universite deToulouse CNRSUPSBP 64182205 route de Nartxonne310077 Toulouse Cedex 04France

Recognition of a pathogen by the plant immune system often triggers a form of regulated cell death traditionally known as the hypersensitive response(HR).This type of cell death occurs precisely at the site of pathogen recognition,and it is restricted to a few *** research has shed light on how plant immune receptors are mechanistically ***,two central key questions remain largely unresolved:how does cell death zonation take place,and what are the mechanisms that underpin this phenomenon?Consequently,bona fide transcriptional indicators of HR are lacking,which prevents deeper insight into its mechanisms before cell death becomes macroscopic and precludes early or live *** this study,to identify the transcriptional indicators of HR we used the paradigmatic Arabidopsis thaliana–Pseudomonas syringae pathosystem and performed a spatiotemporally resolved gene expression analysis that compared infected cells that will undergo HR upon pathogen recognition with bystander cells that will stay alive and activate *** data revealed unique and time-dependent differences in the repertoire of differentially expressed genes,expression profiles,and biological processes derived from tissue undergoing HR and that of its ***,we generated a pipeline based on concatenated pairwise comparisons between time,zone,and treatment that enabled us to define 13 robust transcriptional HR *** these genes,the promoter of an uncharacterized AAA-ATPase was used to obtain a fluorescent reporter transgenic line that displays a strong spatiotemporally resolved signal specifically in cells that will later undergo pathogen-triggered cell *** valuable set of genes can be used to define cells that are destined to die upon infection with HR-triggering bacteria,opening new avenues for specific and/or high-throughput techniques to study HR processes at a single-cell level.

关键词： Arabidopsis thaliana cell death indicator effector-triggered immunity hypersensitive response pattern-triggered immunity plant immunity Pseudomonas syringae

Correlations between corneal and optic nerve head variables in healthy subjects and patients with primary open angle glaucoma

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International Journal of Ophthalmology(English edition) 2015年第6期8卷 1156-1161页

作者： Federico Saenz-Frances Luis Janez Lara Borrego-Sanz Clara Berrozpe-Villabona jose Maria Martinez-de-la-Casa Laura morales-Fernandez Julian Garcia-Sanchez Enrique Santos-Bueso Julian Garcia-Feijoo Department of Ophthalmology Clinico San Carlos HospitalComplutense University of Madrid Technology of Knowledge Institute Complutense University of Madrid

AIMTo correlate corneal variables (determined using the Pentacam) with optic nerve head (ONH) variables determined using the Heidelberg retina tomograph (HRT) in healthy subjects and patients diagnosed with primary open angle glaucoma (POAG).METHODSMeasurements were made in 75 healthy eyes and 73 eyes with POAG and correlations examined through Pearson correlation coefficients between the two sets of variables in the two subject groups. The corneal variables determined were corneal volume (CVol), central corneal thickness (CCT), overall corneal thickness (OvCT), the mean thickness of a circular zone centered at the corneal apex of 1 mm radius (zone I) and the mean thickness of several concentric rings, also centered at the apex until the limbus, each of 1 mm width (zones II to VI respectively). The ONH variables were determined using the *** following pairs of variables were correlated in the control group: CCT-disc area (DAr) (-0.48; P<0.0001), Zone I-DAr (-0.503; P<0.0001) and Zone II-DAr (-0.443; P<0.0001); and in the POAG group: CCT-cup-to-disc area ratio (CDRa) (-0.402; P<0.0001), Zone I-CDRa (-0.418; P<0.0001), Zone II-CDRa (-0.405; P=0.006), Zone I-cup shape measure (CSM) (-0.415; P=0.002), Zone II-CSM (-0.405; P=0.001), Zone IV-height variation contour (HVC) (0.378; P=0.002); Zone V-HVC (0.388, P<0.0001).CONCLUSIONSIn the healthy subjects, significant negative correlation was detected between central and paracentral corneal thickness and optic disc area. In contrast, the POAG patients showed significant negative correlation between central and paracentral corneal thickness and the cup-disc ratio and CSM, and positive correlation between peripheral corneal thickness and HVC.

关键词： glaucoma optic nerve head corneal thickness

Hepatocyte culture in a radial-flow bioreactor with plasma polypyrrole coated scaffolds

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BIOCELL 2015年第2期39卷 9-14页

作者： Odin RAMÍREZ-FERNÁNDEZ Rafael GODÍNEZ Esmeralda ZUÑIGA-AGUILAR Luis E.GÓMEZ-QUIROZ María C.GUTIÉRREZ-ruiz Juan morales Roberto OLAYO Departamento de Ingeniería Eléctrica Universidad Autónoma MetropolitanaUnidad IztapalapaApdo.Postal 55-534IztapalapaMéxico. Departamento de Ciencias de la Salud Universidad Autónoma MetropolitanaUnidad IztapalapaApdo.Postal 55-534IztapalapaMéxico Departamento de Física Universidad Autónoma MetropolitanaUnidad IztapalapaApdo.Postal 55-534IztapalapaMéxico

We have designed and evaluated a radial-flow bioreactor for three-dimensional liver carcinoma cell culture on a new porous coated *** designed a culture chamber where a radial flow of culture medi-um was continuously delivered through *** this system was established,flow was simulated using flow dy-namics software based on numeric methods to solve Navier-Stockes flow *** within cell culture scaffolds was simulated using a flow velocity of 7 mL/min and found that cell culture medium was distributed unhindered in the bioreactor ***,the bioreactor was built according to the simulated design and was tested with liver carcinoma cells(HepG2)cultured over an L-polylactic acid scaffold whose surface was modified with iodine-doped *** bioreactor was tested under non-flow and in radial flow *** density under radial flow conditions was almost double than that under static conditions and both total protein and albumin output was also increased under radial flow conditions.

关键词： Cell Cultures Flow Simulation Flow Features HepG2 Cells Plasma Polymerization

Ambispective comparative study of two surgical strategies for liver hydatidosis

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World Journal of Gastroenterology 2012年第6期18卷 546-550页

作者： jose M Ramia Francisco ruiz-Gomez Roberto De la Plaza Pilar Veguillas jose Quiones Jorge García-Parreo Department of Surgery Hepato-Bilio-Pancreatic Surgery UnitGuadalajara University HospitalGuadalajara 19002Spain

AIM:To investigate the morbidity,mortality,recurrence and technical aspects of two distinct surgical strategies that were implemented in successive ***:Ninty-two patients with 113 cysts underwent surgical *** study was divided into 2 *** from first period(P1) were compiled *** surgical strategy was conservative *** second period(P2) included a prospective study conducted according to a protocol following the criterion that radical procedures should be performed whenever it is technically ***:Patients of both periods showed no statistically significant differences in age,gender,cyst location or *** the P2 group,patients exhibited more preoperative jaundice,and cyst size was smaller(P < 0.05).Changes in surgical strategy increased the rate of radical surgery,decreases morbidity and in-hospital stay(P < 0.001).A negative result in P2 was the death of two old patients(4.8%) who had undergone conser-vative *** rate of radical surgery in P2 was around 75%.CONCLUSION:Radical surgery should be the technique of choice whenever it is feasible,because it diminishes morbidity and in-hospital *** surgery must be employed only in selected cases.

关键词： Hydatid disease Surgery Morbidity Liver

Clinical characteristics and type of antithrombotic treatment in a Spanish cohort of elderly patients with atrial fibrillation according to dependency, frailty and cognitive impairment

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Journal of Geriatric Cardiology 2018年第4期15卷 268-274页

作者： jose Maria Mostaza Manuel Jesús Romero Jiménez Fernando José ruiz Laiglesia José Antonio Díaz Peromingo Manuel Beltrán Robles Ernesto Guevara Sierra Ana Santander Bilbao Carmen Suárez Hospital Carlos III Madrid Spain Hospital Infanta Elena Huelva Spain Hospital Clínico Universitario Lozano Blesa Zaragoza Spain Complejo Hospitalario Universitario de Santiago Santiago de Compostela Spain Hospital Virgen del Camino Sanlucar-Cádiz Spain Centro Socio-Sanitario Vallparadís Terrassa Spain Hospital de Basurto Bilbao Spain Hospital Universitario de La Princesa Madrid Spain

Background Available data regarding clinical profile and management of elderly patients with atrial fibrillation （AF） according to dependency, fragility and cognitive impairment are scarce. The objective of the study was to analyze the biodemographic data, clinical profile and antithrombotic treatment according to dependency, fragility and cognitive impairment in elderly AF patients. Methods Cross- sectional and multi-center study performed in consecutive AF patients ≥ 75 years treated with oral anticoagulants ≥ 3 months attended in Internal Medicine Departments in Spain. Results A total of 837 patients （83.0 ± 5.0 years; CHA2DS2-VASc： 5.0 ± 1.4; HAS-BLED： 2.1 ± 0.9） were included. 44.4% of patients had some degree of dependency, 43.3% were fragile, and 32.3% had cognitive impairment. Patients with any of these conditions were older, had a worse clinical profile, with more comorbidities and higher risks of thromboembolic and bleeding events. All these conditions were independently associated among them. Overall, 70.8% of patients were taking vitamin K antagonists, the remaining 29.2% direct oral anticoagulants and 9.7% oral antiplatelets. This distribution was independent of the presence of dependency or fragility, but there was a trend to a higher prescription of vitamin K antagonists in those patients with cognitive impairment （75.2% vs. 68.8%; P = 0.05）. Conclusions Approximately 32%-44% of elderly anticoagulated AF patients attended have some degree of dependency, fragility and/or cognitive impairment. Patients with any of these conditions are older and have a worse clinical profile. Ap？proximately 71% of patients are taking vitamin K antagonists, regardless dependency or frailty, but with a trend to higher prescription in patients with cognitive impairment.

关键词： Atrial fibrillation Cognitive impairment Dependency Fragility Oral anticoagulants

Photoacoustic Studies of Colloidal Silica Particles after MeV Ion-Induced Shape Deformation

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New Journal of Glass and Ceramics 2011年第2期1卷 63-68页

作者： Ulises morales Rosalba Castaneda-Guzman Santiago Jesus Perez-ruiz Juan Carlos Cheang Wong Instituto de Fisica Universidad Nacional Autonoma de MexicoMexicoD.F.Mexico Centro de Ciencias Aplicadas y Desarrollo Tecnologico Universidad Nacional Autonoma de MexicoMexicoD.F.Mexico

Ordered arrays of colloidal submicrometer-sized silica particles deposited onto silicon wafers were irradiated with MeV Si ions. The spherical silica particles turned into oblate particles as a result of the increase of the particle dimension perpendicular to the ion beam direction and the decrease in the parallel direction. Pulsed laser photoacoustic spectroscopy was used to study the structural changes of the silica particles after the ion-induced shape deformation. Our purpose is to correlate the mechanical vibrations generated by the pulsed laser as a function of the Si irradiation parameters: ion energy and fluence. Fast Fourier transform analysis of the photoacoustic signal was carried out in order to obtain the normal vibration modes of the system. The size, size distribution and shape of the silica particles were determined by scanning electron microscopy. Our results revealed significant structural differences between the spherical and the deformed silica particles.

关键词： Pulsed Laser Photoacoustic Spectroscopy Silica Particles Ion Irradiation

Multivariate Statistical Analysis of Phyllite Samples Based on Chemical(XRF)and Mineralogical Data by XRD

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American Journal of Analytical Chemistry 2012年第5期3卷 347-363页

作者： Eduardo Garzon Garzon Antonio ruiz-Conde Pedro jose Sanchez-Soto Department of Rural Engineering University of AlmeriaLa Canada de San UrbanoSpain Instituto de Ciencia de Materiales Centro Mixto C.S.I.C.—US.Avda.Americo VespuccioSevillaSpain

It is presented the results obtained of a multivariate statistical analysis concerning the chemical and phase composition, as a characterization purpose, carried out with 52 rock phyllite samples selected from the provinces of Almería and Granada (SE Spain). Chemical analysis was performed by X-ray fluorescence (XRF). Crystalline phase analysis was performed by X-ray powder diffraction (XRD) and the mineralogical composition was then deduced. Quantification of weight loss (100? and 1000?C) was carried out by thermal analysis. The aims of this investigation were to analyze and compare the chemical and mineralogical composition of all these samples and to find similarities and differences between them to allow a classification. Several correlations between results of the characterization techniques have been also investigated. All the data have been processed using the multivariate statistical analysis method. The XRF macro-elements (10) and microelements (39) data generate one macrogroup with two new subgroups (1 and 2), and an isolated sample. In subgroup 1 of macroelements, a positive correlation was found between XRF results and geographic location characterized by lower MgO content, which is associated to its geological origins. When multivariate statistical analysis is applied to results obtained by XRD, two groups appear: the first one with a sample with zero percentage of iron oxide and the second one with the rest of the samples, which is classified in two groups. A correlation is observed between the alkaline content (XRF) and illite (XRD), CaO and MgO with dolomite and indirectly between the weight loss after heating at 1000?C and the contents of phase minerals that lose structural water (illite + chlorite) or carbon dioxide (dolomite). The present investigation has interest and implications for geochemistry and analytical chemistry concerning earth rocks and silicate raw materials.

关键词： Selection Multivariate Phyllites XRF XRD

Migration of intra-aortic balloon pump causing obstruction of the superior mesenteric artery

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Journal of Geriatric Cardiology 2022年第11期19卷 928-929页

作者： Sharan Malkani jose ruiz Smruti Desai Smit Paghdar Parag C.Patel Juan Carlos Leoni Moreno Melissa Lyle Maedeh Ganji Daniel Yip Rohan M.Goswami Division of Transplant Cardiology Mayo ClinicJacksonvilleFLUSA Division of Cardiology First Coast Heart&VascularJacksonvilleFLUSA

Axillary intra-aortic balloon pump(IABP)used has increased in the recent few years after UNOs allocation system ***’s design and counterpulsation mechanism is highly regarded for its safety profile,simplicity and relative ease in management and ***,despite continuous innovative efforts,the potential for complications still *** Benchmark registry is widely regarded as the tool for monitoring the evolving practice of *** most common complications reported are balloon perforation,bleeding,migration,limb *** continuous innovative efforts,the potential for complications still exists.

关键词： balloon IABP pump

All-fiber ellipsometer for nanoscale dielectric coatings

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Opto-Electronic Advances 2023年第10期6卷 31-43页

作者： jose Javier Imas Ignacio R.Matías Ignacio Del Villar Aritz Ozcáriz Carlos ruiz Zamarreño Jacques Albert Department of Electrical Electronic and Communications EngineeringPublic University of NavarrePamplona 31006Spain Institute of Smart Cities Public University of NavarrePamplona 31006Spain Department of Electronics Carleton UniversityOttawa(Ontario)K1S 5B6Canada.

Multiple mode resonance shifts in tilted fiber Bragg gratings(TFBGs)are used to simultaneously measure the thickness and the refractive index of TiO_(2) thin films formed by Atomic Layer Deposition(ALD)on optical *** is achieved by comparing the experimental wavelength shifts of 8 TFBG resonances during the deposition process with simulated shifts from a range of thicknesses(T)and values of the real part of the refractive index(n).The minimization of an error function computed for each(n,T)pair then provides a solution for the thickness and refractive index of the deposited film and,a posteriori,to verify the deposition rate throughout the process from the time evolution of the wavelength shift *** of the results were carried out with a conventional ellipsometer on flat witness samples deposited simultaneously with the fiber and with scanning electron measurements on cut pieces of the fiber *** final values obtained by the TFBG(n=2.25,final thickness of 185 nm)were both within 4%of the validation *** approach provides a method to measure the formation of nanoscale dielectric coatings on fibers in situ for applications that require precise thicknesses and refractive indices,such as the optical fiber sensor ***,the TFBG can also be used as a process monitor for deposition on other substrates for deposition methods that produce uniform coatings on dissimilar shaped substrates,such as ALD.

关键词： tilted fiber Bragg grating(TFBG) mode transition ellipsometer