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Regeneration of critical-sized defects, in a goat model, using a dextrin-based hydrogel associated with granular synthetic bone substitute

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Regenerative Biomaterials 2021年第1期8卷 73-82页

作者： Isabel Pereira jose Eduardo Pereira luis Maltez Alexandra Rodrigues Catarina Rodrigues Manuela Oliveira Dina MSilva Ana Rita Caseiro Justina Prada Ana Colette Mauricio jose Domingos Santos Miguel Gama CEB Centre of Biological EngineeingUniversity of MinhoCampus de GualtarBraga 4710-057Portugal CECAV Animal and Veterinary Research CentreUniversity of Trias-os-Montes e Alto DouroVila Real 5001-801Portugal Department of Veterinary Sciences University of Tras-os-Montes e Alto DouroVila Real 5001-801Portugal Bios ckin Molecular and Cell Therapies S.A.Laboratorio CriovidaTecMaiaRua Engenheiro Frederico Ulrich 2650Moreira da Maia 4470-605Portugal Departamento de Clinicas Veterinarias Instituto de Ciencias Biomedicas de Abel Salazar(ICBAS)Universidade do Porto(UP)Rua de Jorge Viterbo Ferreiran°228Porto 4050-313Portugal Centro de Estudos de Ciencia Animal(CECA) Instituto de CienciasTecnologias e Agroambiente da Universidade do Porto(ICETA)Rua D.Manuel IApartado 55142Porto 4051-401 Portugal Centro de Investigaqao Vasco da Gama(CIVG)/Escola Universitaria Vasco da Gama(EUVG) Avenida Jose R.Sousa Femandesn°197 LordemaoCoimbra 3020-210Portugal REQUIMTE/LAQV Departamento de Engenharia Metalurgica e MateriaisFaculdade de EngenhariaUniversidade do PortoRua Dr Roberto FriasPorto 4200-495Portugal

The development of injectable bone substitutes(IBS)have obtained great importance in the bone re-generation field,as a strategy to reach hardly accessible defects using minimally invasive techniques and able to fit to irregular *** this scenario,the association of injectable hydrogels and bone graft granules is emerging as a well-established ***,in situ forming hydrogels have arisen as a new IBS *** in situ forming and injectable dextrin-based hydrogel(HG)was developed,aiming to act as a carrier of granular bone substitutes and bioactive *** this work,the HG was associated to a granular bone substitute(Bonelike)and implanted in goat critical-sized calvarial defects(14mm)for 3,6 and *** results showed that HG improved the han-dling properties of the Bonelike granules and did not affect its osteoconductive features,neither impairing the bone regener ation *** multipotent mesenchymal stromal cells from the umbilical cord,extracellular matrix hydrolysates and the pro-angiogenic peptide LLKKK18 were also combined with the *** bioactive agents did not enhance the new bone formation significantly under the conditions tested,according to micro-computed tomography and histological analysis.

关键词： polysaccharide injec table hydrogel granular ceramics Bonelike' bone regen eration calvarial defect

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Molecular cloning and characterization of a lipid transfer protein gene(PsLTP1) from Pinus sylvestris(L.)

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Journal of Forestry Research 2019年第3期30卷 1149-1158页

作者： Nataliya Hrunyk Valentina Kovaleva Hryhoriy Krynytskyy Ivan Gout Francisco Amil-Ruiz Juan Munoz-Blanco jose luis Caballero Roman Gout Laboratory of Molecular-Genetic Markers in Woody Plants Ukrainian National Forestry UniversityGen. Chuprynka Str. 103Lviv 79057Ukraine Departamento de Bioquimica y Biologia Molecular Campus de Excelencia Internacional Agroalimentario ceiA3-Campus de RabanalesEdificio Severo Ochoa-C6Universidad de Cordoba14071 CordobaSpain Department of Structural and Molecular Biology University College LondonLondon WC1E 6BTUK

Plant nonspecific lipid transfer proteins (nsLTPs) are widely distributed through plant kingdom and are characterized by the presence of a central hydrophobic cavity, suitable for binding various hydrophobic molecules. Despite extensive research on nsLTP in different plant species, mostly angiosperm, and the great diversity of physiological processes in which they seem to be involved, their exact functions still remain unclear. Also, very limited experimental data are available on nsLTP in gymnosperm. In this study, we report for the first time on the molecular cloning of nsLTP, from Pinus sylvestris L.(PsLTP1, GenBank accession JN980402.1) and the expression pattern of PsLTP1 during ontogenesis and in response to environmental stress conditions. Total RNA from roots of 7-day old pine seedlings was used to isolate the cDNA clone, corresponding to Scots pine lipid transfer protein. The open reading frame of PsLTP1 consists of 372 bp encoding a protein of 123 amino acids. Amino acid sequence alignment revealed that mature PsLTP1 shares high level of similarity with nsLTP from other conifers and with well-studied nsLTPs from angiosperms. The PsLTP1 contains a 27-amino-acid N-terminal signal sequence and presents all the features of a plant nsLTP. Amino acid comparison analysis and 3D structure prediction showed that PsLTP1 is a type 1 nsLTP. The results of the expression analysis of Scots pine PsLTP1 gene revealed that its transcripts accumulate in actively growing tissues. Furthermore, transcription of PsLTP1 was upregulated in response to cold and salt treatments, and downregulated during acidic, osmotic and water stresses.

关键词： Scots pine Nonspecific lipid transfer protein (nsLTP) Molecular cloning Expression Abiotic stresses

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Prognosis and follow-up of 135 patients with ischemic colitis over a five-year period

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World Journal of Gastroenterology 2013年第44期19卷 8042-8046页

作者： Angel Cosme Miguel Montoro Santos Santolaria Ana B Sanchez-Puertolas Marta Ponce Margarita Durán jose luis Cabriada Nerea Borda Cristina Sarasqueta luis Bujanda Department of Gastroenterology Hospital de Donostia-Instituto BiodonostiaCentro de Investigaciones Biomédicas en Red en Enfermedades Hepáticas y Digestivas (CIBERehd) Universidad del País Vasco UPV/EHU 20014 San Sebastián Spain Department of Gastroenterology Hospital San Jorge22004 Huesca Spain Department of Gastroenterology Hospital Universitari La Fé 46026 Valencia Spain Department of Gastroenterology Hospital de Galdakao 48960 Bizcaia Spain Institute Biodonostia CIBEResp 20014 San Sebastián Spain

AIM:To study the prognosis(recurrence and mortality)of patients with ischemic colitis(IC).METHODS:This study was conducted in four Spanish hospitals,participants in the Ischemic Colitis in Spain study We analyzed prospectively 135 consecutive patients who met criteria for definitive or probable IC according to Brandt criteria,and follow up these patients during the next five years,***-term results(recurrence and mortality)were evaluated retrospectively after a median interval of 62 mo(range54-75 mo).RESULTS:Estimated IC recurrence rates were 2.9%,5.1%,8.1%and 9.7%at years 1,2,3 and 5 years,***-year survival was 69%(93 of 135)and 24%(10 of 42 patients)died for causes related to the *** these 10 patients,8 died in their first episode at hospital(4 had gangrenous colitis and 4 fulminant colitis)and 2 due to ***:The five-year recurrence rate of IC was *** the other hand,mortality during follow-up was high and was not associated with ischemic colitis.

关键词： Colonic Ischemic Recurrence Follow-up Mortality

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KIR and a specific HLA-C gene are associated with susceptibility and resistance to hepatitis B virus infection in a Brazilian population

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Cellular & Molecular Immunology 2014年第6期11卷 609-612页

作者： Patricia Araujo Giovana Goncalves Flavia Latini Orlando Ferreira luis Cristovao Porto jose Augusto Barreto Manoel Joao Castelo Girao Ricardo Sobhie diaz Colsan - Associacao Beneficente de Coleta de Sangue Sao Paulo SP Brazil Department of Gynecology Federal University ofao Paulo (UNIFESP) Sao Paulo SP Brazil Molecular Virology Laboratory Biology Institute Federal University of Rio de Janeiro (UERJ) Rio de Janeiro R J Brazil Histocompatibility and Cryopreservation Laboratory Federal University of Rio de Janeiro (UERJ) Rio de Janeiro R J Brazil Retrovirology Laboratory Federal University of Sao Paulo (UNIFESP) Sao Paulo SP Brazil

BRIEF REPORT The activity of natural killer （NK） cells is partially regulated by killer cell immunoglobulin-like receptors （KIRs） interacting with human leukocyte antigen C （HLA-C） ligands.1 The ligands of sever... 详细信息

关键词： HLA-C抗原乙型肝炎病毒感染基因配体人口杀伤细胞天然胰岛素人类

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Unveiling Plant mTERF Functions

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Molecular Plant 2012年第2期5卷 294-296页

作者： Pedro Robles jose luis Micol Victor Quesada Instituto de Bioingenieria Universidad Miguel Hernandez Campus de Elche 03202 Elche Spain

Chloroplasts and mitochondria genomes contain a few dozen genes required for gene expression, photosynthesis, and the electron transport chain. However, proteomic and bioinformatic studies suggest that approximately 2000 and 2600 proteins are located in plant mitochondria and chloroplasts, respectively; most of them are encoded by nuclear genes, synthesized in the cytoplasm, and targeted to organelles. Despite the simplicity of organelle genomes, their transcriptional regulation is complex and can only be partially explained by currently known transcriptional machinery components, suggesting that additional unidentified regulatory factors exist （Liere et al., 2011）.

关键词：电子转运钙调蛋白结合蛋白质类光合作用基因基因表达内皮缩血管肽类线粒体叶绿体

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A comparison of process performance during the anaerobic mono-and co-digestion of slaughterhouse waste through different operational modes

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Journal of Environmental Sciences 2018年第2期30卷 149-156页

作者： Jhosané Pagés-Díaz Ileana Pereda-Reyes jose luis Sanz Magnus Lundin Mohammad J.Taherzadeh Ilona Sárvári Horváth Process Engineering Centre (CIPRO) Universidad Tecnológica de La Habana "José Antonio Echeverría" (Technical University of Havana "José A. Echeverría") (Cujae) Swedish Centre for Resource Recovery University of Bor?s Department of Molecular Biology Autonomous University of Madrid

The use of consecutive feeding was applied to investigate the response of the microbial biomass to a second addition of substrates in terms of biodegradation using batch tests as a promising alternative to predict the behavior of the process. Anaerobic digestion（AD） of the slaughterhouse waste（SB） and its co-digestion with manure（M）, various crops（VC）, and municipal solid waste were evaluated. The results were then correlated to previous findings obtained by the authors for similar mixtures in batch and semi-continuous operation modes. AD of the SB failed showing total inhibition after a second feeding. Co-digestion of the SB ＋ M showed a significant improvement for all of the response variables investigated after the second feeding, while co-digestion of the SB ＋ VC resulted in a decline in all of these response variables. Similar patterns were previously detected, during both the batch and the semi-continuous modes.

关键词： Co-digestion Biomethane potential test Second feeding Specific methanogenic activity Slaughterhouse waste

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关于布雷顿森林Ⅱ的思考

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中国金融 2009年第12期 38-40页

作者： jose luis Escriva 西班牙对外银行经济研究部

目前宣布布雷顿森林II崩溃为时尚早,现行国际货币体系的短期前景仍然将由现行制度决定,尽管这一制度已经屡次出现缺陷。但如果缺乏足够的政治领导力进行协调。

关键词：森林布雷国际货币体系制度决定 70年代领导力现行

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明显增强的镧掺杂锶铁氧体的磁光克尔效应

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材料研究学报 2012年第1期26卷 91-95页

作者：朱德如刘先松胡锋 jose luis Menendez 安徽大学物理与材料科学学院磁性材料安徽省工程技术研究中心合肥230039 Universidad de Oviedo Parque Tecnologico de Asturias33428 LlaneraSpain

采用陶瓷法制备了稀土La^(3+)掺杂的锶铁氧体Sr_(1-x)La_xFe_(12)O_(19)(x=0,0.05,0.10,0.15,0.20)。系统地研究了La^(3+)取代对材料结构、磁性能的影响,特别是对磁光克尔效应的影响。实验结果表明,La^(3+)取代Sr^(2+)能显著增强锶铁氧... 详细信息

采用陶瓷法制备了稀土La^(3+)掺杂的锶铁氧体Sr_(1-x)La_xFe_(12)O_(19)(x=0,0.05,0.10,0.15,0.20)。系统地研究了La^(3+)取代对材料结构、磁性能的影响,特别是对磁光克尔效应的影响。实验结果表明,La^(3+)取代Sr^(2+)能显著增强锶铁氧体的磁光克尔效应,当x=0.20时,与SrFe_(12)O_(19)相比,磁光克尔效应增加到了1%。

关键词：无机非金属材料锶铁氧体镧掺杂磁性能磁光克尔效应

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COVID-19 pandemic and nurse teaching:Our experience

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World Journal of Clinical Cases 2022年第8期10卷 2657-2659页

作者： Juan Carlos Molina Ruiz jose luis Guerrero Orriach Maria luisa Bravo Arcas Angela Montilla Sans RocioEscano Gonzalez Department of Anesthesia Hu Virgen De La VictoriaMalaga 29006Spain

In this letter to the editor,we would like to show in our hospital how our nurse team manage formation during coronavirus disease 2019 pandemic.

关键词： COVID-19 Anesthesia Critical care Nurses First wave Training

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Ion channels, phosphorylation and mammalian sperm apacitation

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Asian Journal of Andrology 2011年第3期13卷 395-405,511页

作者： Pablo E Visconti Dario Krapf jose luis de la Vega-Beltran Juan jose Acevedo Alberto Darszon Department of Veterinary and Animal Science Paige Labs University of Massachusets Amherst MA 01003 USA Institute of Molecular and Cell Biology of Rosario （CONICETUNR） and Area Biologia Facultad de Ciencias Biologicas y Farmaceuticas UNR Rosario 2000 Argentina Departamento de Genetica del Desarrollo y Fisiologia Molecular Institute de Biotecnologia Universidad Nacional Autonoma de Mexico （UNAM） Cuemavaca Merelos C.P. 62510 Mexico Departamento de Fisiologia y Farmacologia Facultad de Medicina Universidad Autenoma del Estado de Morelas Cuernavaca C.P. 62510 Mexico

Sexually reproducing animals require an orchestrated communication between spermatozoa and the egg to generate a new individual. Capacitation, a maturational complex phenomenon that occurs in the female reproductive tract, renders spermatozoa capable of binding and fusing with the oocyte, and it is a requirement for mammalian fertilization. Capacitation encompasses plasma membrane reorganization, ion permeability regulation, cholesterol loss and changes in the phosphorylation state of many proteins. Novel tools to study sperm ion channels, image intracellular ionic changes and proteins with better spatial and temporal resolution, are unraveling how modifications in sperm ion transport and phosphorylation states lead to capacitation. Recent evidence indicates that two parallel pathways regulate phosphorylation events leading to capacitation, one of them requiring activation of protein kinase A and the second one involving inactivation of ser/thr phosphatases. This review examines the involvement of ion transporters and phosphorylation signaling processes needed for spermatozoa to achieve capacitation. Understanding the molecular mechanisms leading to fertilization is central for societies to deal with rising male infertility rates, to develop safe male gamete-based contraceptives and to preserve biodiversity through better assisted fertilization strategies.

关键词： capacitation ion channels lipids phosphorylation sperm

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