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Inverted Meckel’s diverticulum diagnosed using capsule endoscopy: A case report

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World Journal of Gastroenterology 2021年第36期27卷 6154-6160页

作者： Ismael El Hajra Martínez Marta Calvo Joséluis Martínez-Porras Lucia Gomez-Pimpollo Garcia jose L Rodriguez Carmen Leon Joséluis Calleja Panero Department of Gastroenterology Hospital Universitario Puerta de HierroMadrid 28222Spain Department of Radiology Hospital Universitario Puerta de HierroMadrid 28222Spain Department of Diagnostic Pathology Hospital Universitario Puerta de HierroMadrid 28222Spain Department of Surgery Hospital Universitario Puerta de HierroMadrid 28222Spain

BACKGROUND Meckel’s diverticulum is a common asymptomatic congenital gastrointestinal ***,its presentation as an inverted Meckel's diverticulum is a rare complication,of which few cases have been reported in the *** SUMMARY Here,we report the case of a 33-year-old man with iron deficiency anemia without manifestation of gastrointestinal *** upper gastrointestinal endoscopy and total colonoscopy were performed,but no abnormalities were found within the observed ***,a capsule endoscopy was performed and offered us a clue to subsequently confirm the diagnosis of inverted Meckel's diverticulum via computed tomography *** intestinal resection surgery was *** final pathology report described a Meckel’s *** Since inverted Meckel's diverticulum is an uncommon disease and its clinical presentation is not specific,it may go undetected by capsule *** diagnosis and treatment of this disease requires a high index of clinical suspicion.

关键词： Inverted Meckel’s diverticulum Capsule endoscopy Anemia study Small bowel tumor Case report

The different root apex zones contribute to drought priming induced tolerance to a reoccurring drought stress in wheat

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The Crop Journal 2021年第5期9卷 1088-1097页

作者： Xiao Wang Jing Chen Jiakun Ge Mei Huang Jian Cai Qin Zhou Tingbo Dai luis Alejandro jose Mur Dong Jiang National Technique Innovation Center for Regional Wheat Production/Key Laboratory of Crop Ecophysiology Ministry of Agriculture/Nanjing Agricultural UniversityNanjing 210095JiangsuChina Institute of Biological Environmental and Rural SciencesAberystwyth UniversityAberystwyth SY233DAUK

Drought priming is a promising approach to improve tolerance to further drought in *** root apex plays important roles in drought however,its contribution to drought priming remains *** provide mechanistic insights into this process,the transcriptomes and proteomes at three different zones along the root axis under drought stress were *** assessment of root growth indicated that priming augmented roots growth in response to drought and also the levels of protective proline and glycine *** across the proximal to the distal zones of the root apex indicated increases the transcription of genes involved in primary and secondary ***,genes related to translation,transcription,folding,sorting and degradation,replication and repair were increased in the apex compared to the proximal zone.A single drought episode suppressed their expression but prior drought priming served to maintain expression with recurrent drought *** differentially primed responses genes were mainly involved in the pathways related to plant hormone signaling,stress defense and cell wall *** prediction of regulatory hubs using Cytoscape implicated signaling components such as the ABA receptor PYL4 as influencing antioxidant status and the cell *** our integrative transcriptomic-proteomic assessments we present a model for drought priming protected plant hormone signaling transduction pathways to drive the cell cycle and cell wall loosening to confer beneficial effects on roots to counter the effects of *** model provides a theoretical basis for improvement of drought tolerance in wheat,via an increased understanding of drought priming induced drought tolerance.

关键词： Wheat Drought priming Drought tolerance Root Transcriptome

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Molecular cloning and characterization of a lipid transfer protein gene(PsLTP1) from Pinus sylvestris(L.)

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Journal of Forestry Research 2019年第3期30卷 1149-1158页

作者： Nataliya Hrunyk Valentina Kovaleva Hryhoriy Krynytskyy Ivan Gout Francisco Amil-Ruiz Juan Munoz-Blanco jose luis Caballero Roman Gout Laboratory of Molecular-Genetic Markers in Woody Plants Ukrainian National Forestry UniversityGen. Chuprynka Str. 103Lviv 79057Ukraine Departamento de Bioquimica y Biologia Molecular Campus de Excelencia Internacional Agroalimentario ceiA3-Campus de RabanalesEdificio Severo Ochoa-C6Universidad de Cordoba14071 CordobaSpain Department of Structural and Molecular Biology University College LondonLondon WC1E 6BTUK

Plant nonspecific lipid transfer proteins (nsLTPs) are widely distributed through plant kingdom and are characterized by the presence of a central hydrophobic cavity, suitable for binding various hydrophobic molecules. Despite extensive research on nsLTP in different plant species, mostly angiosperm, and the great diversity of physiological processes in which they seem to be involved, their exact functions still remain unclear. Also, very limited experimental data are available on nsLTP in gymnosperm. In this study, we report for the first time on the molecular cloning of nsLTP, from Pinus sylvestris L.(PsLTP1, GenBank accession JN980402.1) and the expression pattern of PsLTP1 during ontogenesis and in response to environmental stress conditions. Total RNA from roots of 7-day old pine seedlings was used to isolate the cDNA clone, corresponding to Scots pine lipid transfer protein. The open reading frame of PsLTP1 consists of 372 bp encoding a protein of 123 amino acids. Amino acid sequence alignment revealed that mature PsLTP1 shares high level of similarity with nsLTP from other conifers and with well-studied nsLTPs from angiosperms. The PsLTP1 contains a 27-amino-acid N-terminal signal sequence and presents all the features of a plant nsLTP. Amino acid comparison analysis and 3D structure prediction showed that PsLTP1 is a type 1 nsLTP. The results of the expression analysis of Scots pine PsLTP1 gene revealed that its transcripts accumulate in actively growing tissues. Furthermore, transcription of PsLTP1 was upregulated in response to cold and salt treatments, and downregulated during acidic, osmotic and water stresses.

关键词： Scots pine Nonspecific lipid transfer protein (nsLTP) Molecular cloning Expression Abiotic stresses

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Regeneration of critical-sized defects, in a goat model, using a dextrin-based hydrogel associated with granular synthetic bone substitute

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Regenerative Biomaterials 2021年第1期8卷 73-82页

作者： Isabel Pereira jose Eduardo Pereira luis Maltez Alexandra Rodrigues Catarina Rodrigues Manuela Oliveira Dina MSilva Ana Rita Caseiro Justina Prada Ana Colette Mauricio jose Domingos Santos Miguel Gama CEB Centre of Biological EngineeingUniversity of MinhoCampus de GualtarBraga 4710-057Portugal CECAV Animal and Veterinary Research CentreUniversity of Trias-os-Montes e Alto DouroVila Real 5001-801Portugal Department of Veterinary Sciences University of Tras-os-Montes e Alto DouroVila Real 5001-801Portugal Bios ckin Molecular and Cell Therapies S.A.Laboratorio CriovidaTecMaiaRua Engenheiro Frederico Ulrich 2650Moreira da Maia 4470-605Portugal Departamento de Clinicas Veterinarias Instituto de Ciencias Biomedicas de Abel Salazar(ICBAS)Universidade do Porto(UP)Rua de Jorge Viterbo Ferreiran°228Porto 4050-313Portugal Centro de Estudos de Ciencia Animal(CECA) Instituto de CienciasTecnologias e Agroambiente da Universidade do Porto(ICETA)Rua D.Manuel IApartado 55142Porto 4051-401 Portugal Centro de Investigaqao Vasco da Gama(CIVG)/Escola Universitaria Vasco da Gama(EUVG) Avenida Jose R.Sousa Femandesn°197 LordemaoCoimbra 3020-210Portugal REQUIMTE/LAQV Departamento de Engenharia Metalurgica e MateriaisFaculdade de EngenhariaUniversidade do PortoRua Dr Roberto FriasPorto 4200-495Portugal

The development of injectable bone substitutes(IBS)have obtained great importance in the bone re-generation field,as a strategy to reach hardly accessible defects using minimally invasive techniques and able to fit to irregular *** this scenario,the association of injectable hydrogels and bone graft granules is emerging as a well-established ***,in situ forming hydrogels have arisen as a new IBS *** in situ forming and injectable dextrin-based hydrogel(HG)was developed,aiming to act as a carrier of granular bone substitutes and bioactive *** this work,the HG was associated to a granular bone substitute(Bonelike)and implanted in goat critical-sized calvarial defects(14mm)for 3,6 and *** results showed that HG improved the han-dling properties of the Bonelike granules and did not affect its osteoconductive features,neither impairing the bone regener ation *** multipotent mesenchymal stromal cells from the umbilical cord,extracellular matrix hydrolysates and the pro-angiogenic peptide LLKKK18 were also combined with the *** bioactive agents did not enhance the new bone formation significantly under the conditions tested,according to micro-computed tomography and histological analysis.

关键词： polysaccharide injec table hydrogel granular ceramics Bonelike' bone regen eration calvarial defect

Proposal for a novel and simple WDM NRZ-DPSK system

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Frontiers of Optoelectronics 2009年第3期2卷 253-258页

作者： Yu YU Xinliang ZHANG jose B.rosas-FERNáNDEZ Dexiu HUANG Richard V.PENTY Ian H.WHITE Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Centre for Photonic Systems Electrical Engineering Division Engineering Department University of Cambridge

A novel and simple non-return-to-zero differential phase shift keying(NRZ-DPSK) wavelength division multiplexing(WDM) system, which can simultaneously demultiplex and demodulate multiple wavelengths, is proposed and investigated in this paper. The phase-to-intensity demodulation principle is based on detuned filtering, which is achieved by using a single commercial array waveguide grating(AWG) in our scheme. By properly choosing appropriate AWG channels at the transmitter, the AWG at the receiver can act as both the demultiplexer and the demodulator of the DPSK signals. Simulations at 10, 20, and 40 Gbit/s show good flexibility and performance for the proposed system.

关键词： non-return-to-zero differential phase shift keying (NRZ-DPSK) wavelength division multiplexing (WDM) array waveguide grating (AWG) demodulation filtering

A comparison of process performance during the anaerobic mono-and co-digestion of slaughterhouse waste through different operational modes

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Journal of Environmental Sciences 2018年第2期30卷 149-156页

作者： Jhosané Pagés-Díaz Ileana Pereda-Reyes jose luis Sanz Magnus Lundin Mohammad J.Taherzadeh Ilona Sárvári Horváth Process Engineering Centre (CIPRO) Universidad Tecnológica de La Habana "José Antonio Echeverría" (Technical University of Havana "José A. Echeverría") (Cujae) Swedish Centre for Resource Recovery University of Bor?s Department of Molecular Biology Autonomous University of Madrid

The use of consecutive feeding was applied to investigate the response of the microbial biomass to a second addition of substrates in terms of biodegradation using batch tests as a promising alternative to predict the behavior of the process. Anaerobic digestion（AD） of the slaughterhouse waste（SB） and its co-digestion with manure（M）, various crops（VC）, and municipal solid waste were evaluated. The results were then correlated to previous findings obtained by the authors for similar mixtures in batch and semi-continuous operation modes. AD of the SB failed showing total inhibition after a second feeding. Co-digestion of the SB ＋ M showed a significant improvement for all of the response variables investigated after the second feeding, while co-digestion of the SB ＋ VC resulted in a decline in all of these response variables. Similar patterns were previously detected, during both the batch and the semi-continuous modes.

关键词： Co-digestion Biomethane potential test Second feeding Specific methanogenic activity Slaughterhouse waste

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Colorectal cancer prognosis twenty years later

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World Journal of Gastroenterology 2010年第7期16卷 862-867页

作者： luis Bujanda Cristina Sarasqueta Elisabeth Hijona Lander Hijona Angel Cosme Ines Gil jose luis Elorza jose I Asensio Santiago Larburu José M Enríquez-Navascués Rodrigo Jover Francesc Balaguer Xavier Llor Xavier Bessa Montserrat Andreu Artemio Paya Antoni Castells Gastrointestinal Oncology Group of the Spanish Gastroenterological Association Department of Gastroenterology Donostia HospitalUniversity of Basque CountryCentro de Investigación Biomédica en Enfermedades Hepáticas y Digestivas(CIBEREHD)20014 San SebastiánSpain Department of Epidemiology Donostia HospitalCIBERESP20014 San SebastiánSpain Department of Surgery Donostia Hospital20014 San SebastiánSpain Department of Gastroenterology General Hospital Department of Gastroenterology Clinic HospitalCIBEREHDIDIBAPS08036 BarcelonaSpain Digestive Diseases and Nutrition Secion University of IllinoisChicagoIL 60607United States Department of Gastroenterology Del Mar Hospital08003 BarcelonaSpain Department of Gastroenterology General Hospital 03010 AlicanteSpain

AIM:To evaluate changes in colorectal cancer(CRC) survival over the last 20 ***:We compared two groups of consecutive CRC patients that were prospectively recruited:Group Ⅰincluded 1990 patients diagnosed between 1980 and ***Ⅱincluded 871 patients diagnosed in ***:The average follow up time was 21 mo(1-229)for GroupⅠand 50 mo(1-73.4)for GroupⅡ.Overall median survival was significantly longer in Group Ⅱthan in GroupⅠ(73 mo vs 25 mo,P<0.001)and the difference was significant for all tumor *** surgical mortality was 8% for GroupⅠand 2% for Group Ⅱ(P<0.001).Only 17% of GroupⅠpatients received chemotherapy compared with 50% of GroupⅡpatients(P<0.001).CONCLUSION:Survival in colorectal cancer patients has doubled over the past 20 *** increase seems to be partly due to the generalization in the administration of chemotherapy and to the decrease of post surgical mortality.

关键词： Colon cancer Prognosis Survival Chemotherapy Surgery

COVID-19 pandemic and nurse teaching:Our experience

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World Journal of Clinical Cases 2022年第8期10卷 2657-2659页

作者： Juan Carlos Molina Ruiz jose luis Guerrero Orriach Maria luisa Bravo Arcas Angela Montilla Sans RocioEscano Gonzalez Department of Anesthesia Hu Virgen De La VictoriaMalaga 29006Spain

In this letter to the editor,we would like to show in our hospital how our nurse team manage formation during coronavirus disease 2019 pandemic.

关键词： COVID-19 Anesthesia Critical care Nurses First wave Training

Pathogenesis of occult chronic hepatitis B virus infection

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World Journal of Gastroenterology 2011年第12期17卷 1543-1548页

作者： Rocio Aller de la Fuente María L Gutiérrez Javier Garcia-Samaniego Conrado Fernández-Rodriguez jose luis Lledó Gregorio Castellano Gastroenterology Hospital Clinico Universitario Valladolid Institute of endocrinology and Nutrition Medical SchoolUniversity of Valladolid47003 ValladolidSpain Service of Gastroenterology Hospital Universitario Fundación AlcorcónAv Budapest-128922 AlcorcónMadridSpain Gastroenterology Department Hospital Carlos IIIc/Sinesio Delgado28029 MadridSpain Hospital Universitario 12 de Octubre Av de Andalucia 2804128039 MadridSpain

Occult hepatitis B infection(OBI) is characterized by hepatitis B virus(HBV) DNA in serum in the absence of hepatitis B surface antigen(HBsAg) presenting HBsAg-negative and anti-HBc positive serological *** HBV status is associated in some cases with mutant viruses undetectable by HBsAg assays;but more frequently it is due to a strong suppression of viral replication and gene *** is an entity with world-wide *** failure to detect HBsAg,despite the persistence of the viral DNA,is due in most cases to the strong suppression of viral replication and gene expression that characterizes this"occult"HBV infection;although the mechanisms responsible for suppression of HBV are not well *** majority of OBI cases are secondary to overt HBV infection and represent a residual low viremia level suppressed by a strong immune response together with histological derangements which occurred during acute or chronic HBV *** evidence suggests that it can favour the progression of liver fibrosis and the development of hepatocellular carcinoma.

关键词： Occult hepatitis B virus infection Hepatitis B virus-DNA Anti-HBc alone Hepatitis B virus Hepadnaviral hepatitis Occult viral persistence Primary occult infection Secondary occult infection Virus reactivation