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Different culture conditions applied to in vitro shoot multiplication of two Eucalyptus benthamii explant sources

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Journal of Forestry Research 2020年第3期31卷 857-869页

作者： Natalia Pimentel Esposito-Polesi Leandro Silva de oliveira Francisco jose Benedini Baccarin Cristina Vieira de Almeida Marcilio de Almeida Biological Science department "Luiz de Queiroz"Superior College of AgricultureUniversity of Sao Paulo(ESALQ/USP)Padua Dias Avenue11P.O.Box 9PiracicabaSP 13418-900Brazil Institute of Agriculture Sciences Federal University of Minas Gerais(UFMG)Universitaria Avenue1000Montes ClarosMG 39404-547Brazil State University of Goias(UEG) Rua S7Qd.1S SulPalmeiras de GoiasGO 76190-000Brazil In vitro Palm Consultoria Estudo e Desenvolvimento Biologico LtdaRua Itajobi421PiracicabaSP 13432-000Brazil

Eucalyptus adult material requires more successive subcultures in the in vitro multiplication phase for increased vigor and cellular activity. This study evaluated the endophytic manifestation and shoot multiplication of one 13-year-old Eucalyptus benthamii clone under different culture conditions and used canopy branches(CB) and trunk base material as explant sources. The culture media were wood plant medium(WPM), Murashige and Skoog medium(MS) and JADS(Correia and co-authors medium).Based on the results of the initial multiplication experiment, further tests examined sucrose concentrations and p H. Morphophysiology, dry mass production, endophyticmanifestation and histochemical were determined. Explant sources responded differently to MS and JADS media, but the WPM medium promoted homogeneous *** responses were similar for both explant sources when sucrose concentrations varied. Shoots died in the absence of sucrose, showed high oxidation at 60 g L-1 and optimal development at 30 g L-1. Endophytes were more evident for shoots from the CB origin. Explant sources responded distinctively to treatment due to physiological and intrinsic genetic factors. Therefore, explant sources, different culture media, sucrose concentration and p H may determine micropropagation success and influence the presence and/or intensity of endophytic manifestation.

关键词： Plant tissue culture Explant sources Culture media Sucrose pH

Coating independent cytotoxicity of citrate-and PEG-coated silver nanoparticles on a human hepatoma cell line

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Journal of Environmental Sciences 2017年第1期29卷 191-201页

作者： Veronica Bastos jose M.P.Ferreira-de-oliveira Joana Carrola Ana L.Daniel-da-Silva Iola F.Duarte Conceicao Santos Helena oliveira CESAM&Laboratory of Biotechnology and Cytomics Department of BiologyUniversity of Aveiro3810-193 AveiroPortugal CICECO-Aveiro Institute of Materials Department of ChemistryUniversity of Aveiro3810-193 AveiroPortugal department of Biology Faculty of SciencesUniversity of Porto4169-007 PortoPortugal

The antibacterial potential of silver nanoparticles（AgNPs） resulted in their increasing incorporation into consumer,industrial and biomedical ***,human and environmental exposure to AgNPs（either as an engineered product or a contaminant）supports the emergent research on the features conferring them different toxicity *** this study,30 ran AgNPs coated with citrate or poly（ethylene glycol）（PEG） were used to assess the influence of coating on the effects produced on a human hepatoma cell line（HepG2）,namely in terms of viability,apoptosis,apoptotic related genes,cell cycle and cyclins gene *** types of coated AgNPs decreased cell proliferation and viability with a similar toxicity *** the concentrations used（11 and 5 μg/mL corresponding to IC50 and-IC10 levels,respectively） the amount of cells undergoing apoptosis was not significant and the apoptotic related genes BCL2（anti-apoptotic gene）and BAX（pro-apoptotic gene） were both ***,both AgNPs affected HepG2 cell cycle progression at the higher concentration（11 μg/mL） by increasing the percentage of cells in S（synthesis phase） and G2（Gap 2 phase） *** the cell-cycle related genes,the expression of cyclin B1 and cyclin E1 genes were ***,this work has shown that citrate- and PEG-coated AgNPs impact on HepG2 apoptotic gene expression,cell cycle dynamics and cyclin regulation in a similar *** research is needed to determine the properties that confer AgNPs at lower toxicity,since their use has proved helpful in several industrial and biomedical contexts.

关键词： Silver nanoparticles(AgNPs) Citrate Polyethylene glycol)(PEG) HepG2 cells Cytotoxicity Nanotoxicology

Unlocking the in vitro anti-Trypanosoma cruzi activity of halophyte plants from the southern Portugal

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Asian Pacific Journal of Tropical Medicine 2016年第8期9卷 714-719页

作者： Marta oliveira Policarpo Ademar Sales junior Maria Joao Rodrigues Marina dellaGreca Luisa Barreira Silvane Maria Fonseca Murta Alvaro jose Romanha Luisa Custodio Center of Marine Sciences (CCMAR) University of AlgarveCampus de Gambelas 8005-139 FaroPortugal Centro de Pesquisas Rene Rachou FIOCRUZ Belo HorizonteMinas GeraisBrazil Dipartimento di Chimica Organica e Biochimica Universita` Federico Ⅱ Complesso Universitario Monte Sant'AngeloVia Cintia 4I-80126NapoliItaly

Objective:To evaluate the in vitro anti-Trypanosoma cruzi（***） activity of organic extracts prepared from halophyte species collected in the southern coast of Portugal（Algarve）,and chemically characterize the most active ***:Acetone,dichloromethane and methanol extracts were prepared from 31 halophyte species and tested in vitro against trypomastigotes and intracellular amastigotes of the Tulahuen strain of *** most active extract was fractionated by preparative HPLC-DAD,affording 11 *** most selective fraction was fully characterized by ***:From 94 samples tested,one was active,namely the root dichloromethane extract of Juncus acutus(IC50 dentified in fraction 8 are likely responsible for the observed anti parasitic *** research is in progress aiming to isolate and identify the specific active *** the best of our knowledge,this is the first report on the in vitro anti *** activity of halophyte species.

关键词： Chagas disease Halophytes Trypanosoma cruzi Phenanthrenes Juncunol

Autoregressive spatial analysis and individual tree modeling as strategies for the management of Eremanthus erythropappus

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Journal of Forestry Research 2016年第3期27卷 595-603页

作者： Henrique Ferraco Scolforo jose Roberto Soares Scolforo jose Marcio de Mello Anto nio Carlos Ferraz Filho Diogo Francisco Rossoni Thiza Falqueto Altoe Antonio Donizette oliveira Renato Ribeiro de Lima department of Forest Science Federal University of Lavras PO Box: 3037 Lavras MG 37200-000 Brazil department of Statistics State University of Maringa Av. Colombo 5.790 Jd. Universitario Bloco E90 Maringa PR 87020-900 Brazil department of Statistics Federal University of Lavras PO Box: 3037 Lavras MG 37200-000 Brazil

The objectives of this study were to apply statistical techniques to discriminate fertilization treatments of Eremanthus erythropappus（DC.） Mac *** autoregressive modeling, and to develop individual tree models for diameter and crown area（CA） projection to define management strategies for candeia plantations subjected to different fertilization treatments. This is an important tree species originating from the Brazilian Atlantic Rain forest and Savannah biomes, intensively used in the cosmetic industry. Nonetheless, to date, research has not addressed the management of natural stands or plantations of the species. Our experiment was located in Baependi, Minas Gerais, Brazil, and comprised of four randomized blocks and 13 treatments. The treatments consisted of 12 different regimes of fertilization plus a control. Each sample plot was composed of 50 plants plus two border plants in a planting spacing of 2.5 9 2.0 m and undergoing pruning at 5 and 6 years of age. Starting in the second year, total tree height（H） and circumference（at 1.30 m from the ground or breast height, CBH） were measured every 6 months. Starting in the fifth year CA was measured. Tree growth varied by fertilization *** were detected by using an autoregressive approach, considering that standard statistical methods were not powerful enough to detect significant *** growth groups were formed, and maximum growth was obtained for treatment 10（NPK, 8-28-16）. Management guidelines are provided based on individual tree models for different fertilization levels.

关键词： Management strategies Candeia Fertilization level Brazil Crown area

Therapeutic and biological activities of Calotropis procera(Ait.) ***.

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Asian Pacific Journal of Tropical Medicine 2010年第4期3卷 332-336页

作者： Marcia Calheiros Chaves Silva Antonio Batista da Silva Fabiano Moura Teixeira Paulo Cesar Pereira de Sousa Regina Maria Macedo Rondon jose Eduardo Ribeiro Honorio junior Luiz Rafael Leite Sampaio Samanda Lima oliveira Angela Nadyla Martins Holonda Silvania Maria Mendes de Vasconcelos Enfermeira Mestre em Farmacologia/Universidade Federal do Ceara/UFC Farmaceutico Mestre em Patologia Tropical/Universidade Federal do Amazonas/UFAM Biologo Mestre em Bioquimica/Universidade Federal do Ceara/UFC Farmaceutico Mestre em Ciencias Farmaceutica/Universidade Federal do Ceara/UFC Biologa Mestre em Producao Animal/Universidade Estadual do Ceara/UECE Biologo Mestre em Fisiologia da Universidade Estadual do Ceara/UECE Enfermeiro Mestrando em Farmacologia da Universidade Federal do Ceara/UFC Aluna de Farmacia da Universidade Federal do Ceara/UFC Enfermeira Professora Adjunto do Depto.de Fisiologia e Farmacologia da Universidade Federal do Ceara/UFC

Medicinal plants have been used to treat various ailments of the poor population around the world;hence the interest among researchers to know the active ingredients of certain plants has being *** Calotropis procera(***) is a plant original from Africa,commonly found in northeastern *** is well known for their pharmacological properties,since it produces large amounts of *** important role that medicinal plants play in folk medicine has led us to develop this article in order to review the major pharmacological activities of C. procera.

关键词： Calotropis procera Pharmacological activity Medicinal plants

CFD predictions for hazardous area classification

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Chinese Journal of Chemical Engineering 2019年第1期27卷 21-31页

作者： Andrey oliveira de Souza Aurélio Moreira Luiz Ant?nio Tavernard Pereira Neto Ant?nio Carlos Brandao de Araujo Heleno Bispo da Silva Sidinei Kebler da Silva jose Jailson Nicacio Alves Federal Institute of Education Science and Technology of Paraiba Petrobras-Petróleo Brasileiro S.A. Federal University of Campina Grande

This study aimed to describe a Computational Fluid Dynamics(CFD) procedure using the ANSYS CFX software 16.1 and design of Experiments for the determination of volume and extension of explosive atmospheres due to fugitive emissions of flammable *** multidimensional statistical sampling technique Latin Hypercube was used, which defined 100 simulations of random methane gas leak *** CFD model proved to be robust in predicting the extension and volume of the explosive atmosphere for orifice diameters from 0.1 to2.5 mm, pressure from 0.1 MPa to 12 MPa and temperatures from 0 ℃ to 400 ℃.It was found that the calculation domain must be parameterized 8 m in length for each millimeter of the diameter of the source of release to ensure the *** order not to lose precision for very small diameters, the mesh was parameterized with 50 elements along the orifice *** was proved that gravity does not influence the extension and volume of the explosive atmosphere at sonic *** deviation from the ideal gas behavior in the reservoir,achieved by applying the Soave–Redlich–Kwong equation of state, also has not significantly influenced the extension and volume of the explosive *** results showed that the size of the explosive atmosphere varies directly with the diameter of the source emission and reservoir pressure, and inversely with the temperature of the *** diameter of the source is the parameter that has the major effect on the extension of the explosive atmosphere, followed by the pressure and lastly the temperature of the reservoir.

关键词： Computational Fluid Dynamics Computer simulation Safety Fugitive emissions Classified area Flammable gas cloud

International NeuroAIDS: prospects of HIV-1 associated neurological com-plications

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Cell Research 2005年第Z1期15卷 962-969页

作者： J Roberto TRUJILLO Gilberto JARAMILLO-RANGEL Marta ORTEGA-MARTINEZ Augusto C PENALVA de oliveira jose E VIDAL joseph BRYANT Robert C GALLO Institute of Human Virology University of Maryland Biotechnology Institute 725 W Lombard Street Baltimore MD 21201 USA Institute of Infectious Diseases Emilio Ribas Sao Paulo 01246 Brazil Clinical Research Unit in Human Retrovirology University of CampinasSao Paulo 01246 Brazil. Institute of Infectious Diseases Emilio Ribas Sao Paulo 01246 Brazil

Neurological complications associated with HIV-1/AIDS are being recognized with a high frequency that parallels the increased number of AIDS cases. The early infiltration by HIV-1 into the nervous system can cause primary and/or secondary neurological complications. The most common neurocognitive disorder is AIDS dementia Complex (ADC). In developing countries of Asia the three most opportunistic infections are tuberculosis (TB), cryptococcosis, and Pneumocystis carinii pneumonia. Therefore, it is expected that secondary neurological complications due to TB and cryptococcosis will be the most common cause of morbility and mortality in HIV-1/AIDS cases in China. Research of NeuroAIDS in China is necessary to understand the impact and the biology of HIV-1 in the nervous system. Future studies would include, the molecular epidemiology and the description of opportunistic infections associated to HIV-1; the neuropathological description of primary and secondary HIV-1 complications in different groups; the HIV-1 neurot- ropism and immune response studies for China’s unique HIV-1 strains and recombinant forms derived from the nervous system, including experimental models such as the use of transgenic rats; and the study of potential resistant virus, primarily when the anti-retroviral therapy (ART) has not full access in the brain.

关键词： NeuroAIDS HIV-1 neurotropism AIDS dementia complex.

Artificial equilibrium points and bi-impulsive maneuvers to observe 243 Ida

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Chinese Journal of Aeronautics 2021年第1期34卷 410-423页

作者： Allan Kardec de ALMEIDA Jr Geraldo Magela Couto oliveira Antonio Fernando Bertachini de Almeida PRADO National Institute for Space Research(INPE) Sao Jose dos Campos 12227-010Brazil Federal Center for Technological Education of Minas Gerais(CEFET-MG) Belo Horizonte 30421-169Brazil

In the restricted three-body problem,the traditional Lagrange points L1 and L2 are the only equilibrium points near the asteroid 243 *** thrust generated by a solar sail over a spacecraft enables the existence of new artificial equilibrium points,which depend on the position of the spacecraft with respect to the asteroid and the attitude of the solar *** equilibrium points generate new spots to observe the body from above or below the plane of *** points are very good observational locations due to their stationary *** work provides a preliminary analysis to observe Ida through the use of artificial equilibrium points as spots combined with transfer maneuvers between *** combination can be used to observe the asteroid from more different points of view in comparison to fixed *** analyses are made for a spacecraft equipped with a solar sail and capable of performing bi-impulsive *** solar radiation pressure is used both to maintain the equilibrium condition and to reduce the costs of the transfers and/or to create transfers with longer *** is a new aspect of the present research,because it combines the continuous thrust with initial and final small impulses,which are feasible for most of the spacecraft,because the magnitudes of the impulses are very *** combined maneuvers may reduce the transfer times of the maneuvers in most of the cases,compared with the maneuvers based only on continuous *** options involved in these transfers are shown,like to minimize the fuel spent(Dv)as a function of the transfer time or to extend the duration of the travel between the *** transfer times can be useful when observations are required during the transfers.

关键词： Artificial equilibrium points Astrodynamics Orbital transfers Solar radiation pressure Solar sail

Evaluation of physicochemical properties as supporting information on quality control of raw materials and veterinary pharmaceutical formulations

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Journal of Pharmaceutical Analysis 2018年第3期8卷 168-175页

作者： Sara da Silva Anacleto Marcella Matos Cordeiro Borges Hanna Leijoto de oliveira Andressa Reis Vicente Eduardo Costa de Figueiredo Marcone Augusto Leal de oliveira Barbara Juliana Pinheiro Borges Marcelo Antonio de oliveira Warley de Souza Borges Keyller Bastos Borges departamento de Ciencias Naturals Universidade Federal de Sao Joao del-Rei Campus Dom Bosco Praca Dora Helvecio 74 Fabricas 36301-160 Sao Joao del-Rei Minas Gerais Brazil departamento de Quimica Universidade Federal do Espirito Santo Avenida Fernando Ferrari 514 Goiabeiras 29075-910 Vitdria Espirito Santo Brazil Laboratorio de Analise de Toxicantes e Farmacos Faculdade de Ciencias Farmaceuticas Universidade Federal de Alfenas Rua Gabriel Monteiro da Silva 700 Centro 37130-000 Alfenas Minas Gerais Brazil departamento de Quimica Universidade Federal de Juiz de Fora Rua Jose Lourenco Kelmer s/n 36036-330 Juiz de Fora Minas Gerais Brazil Centro de Ciencias da Saude Universidade Federal do Espirito Santo Avenida Marechal Campos 1468 Maruipe 29.043-900 Vitori Espirito Santo Brazil Centro Universitario Norte do Espirito Santo Universidade Federal do Espirito Santo Br 101 Norte Km 67 Sao Mateus Espirito Santo Brazil

This study aimed to show that the physicochemical proprieties obtained by Fourier transform infrared spectroscopy （FTIR）, thermogravimetry （TG）, and scanning electronic microscopy （SEM） can be useful tools for evaluating the quality of active pharmaceutical ingredients （APIs） and pharmaceutical products. In addition, a simple, sensitive, and efficient method employing HPLC-DAD was developed for simulta- neous determination of lidocaine （LID）, ciprofloxacin （CFX） and enrofloxacin （EFX） in raw materials and in veterinary pharmaceutical formulations. Compounds were separated using a Gemini Cm （250 mm×4.6 mm, 5μm） Phenomenex column, at a temperature of 25℃, with a mobile phase containing 10 mM of phosphoric acid （pH 3.29）： acetonitrile （85.7：14.3, v/v） and a flow rate of 1.5 mL/min. Physicochemical characterization by TG, FTIR, and SEM of raw materials of LID, CFX, and EFX provided information useful for the evaluation, differentiation, and qualification of raw materials. Finally, the HPLC method was proved to be useful for evaluation of raw material and finished products, besides satisfying the need for an analytical method that allows simultaneous determination of EFX, CFX, and LID, which can also be extended to other matrices and applications.

关键词： HPLC Physicochemical characterization Quality control Veterinary pharmaceutical formulation Raw material