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Effect of Chitosaccharides in Nodulation and Growth in Vitro of Inoculated Soybean

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American Journal of Plant Sciences 2016年第9期7卷 1380-1391页

作者： Daimy Costales Alejandro Bernardo Falcón María Caridad Nápoles Julien de Winter Pascal Gerbaux Robertus Cornelis Adrianus Onderwater Ruddy Wattiez Juan carlos Cabrera Grupo de Productos Bioactivos Departamento de Fisiología y Bioquímica Vegetal Instituto Nacional de Ciencias Agrícolas (INCA) San José de las Lajas Cuba Interdisciplinary Center for Mass Spectrometry Organic Synthesis and Mass Spectrometry Laboratory University of Mons Unité Biotechnologie Ghislenghiem Belgium Unité Biotechnologie Materia Nova ASBL Ghislenghien Belgium Biosciences Research Institute Department of Proteomics and Microbiology University of Mons—UMONS Mons Belgium

The influence of chitosaccharides on the symbiotic interaction between Bradyrhizobium and soybean was examined. The results show that chitosaccharides either positively or negatively affect soybean nodulation or plant growth depending on their molecular weight, concentration and the application methods. When directly added to the in vitro culture media, chitosan of high molecular weight inhibit Bradyrhizobium viability in a dose dependent manner while chitooligosaccharides reduce slightly the bacteria viability only at concentration equal or higher than 50 mg·L-1. Chitooligosaccharides significantly enhance nodule formation and dry mass in soybean roots at doses between 10 and 100 mg·L-1. Both types of chitosaccharides, at the highest doses (>500 mg·L-1), negatively affect plant height and root size, whereas medium doses (50 to 100 mg·L-1) increase slightly leave number. Under field conditions, foliar application of both chitosaccharides enhances growth and nodulation of soybean plants. Nevertheless, using this application method, chitosan remains more effective than chitooligosaccharides.

关键词： Chitosan Glycine max Bradyrhizobium Antimicrobial

Natural ECM-Bacterial Cellulose Wound Healing—Dubai Study

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Journal of Biomaterials and Nanobiotechnology 2015年第4期6卷 237-246页

作者： Safwat Mohd. El-Hoseny Pierre Basmaji Gabriel Molina de Olyveira Ligia Maria Manzine Costa Abdulwahid M. Alwahedi josé Domingos da Costa Oliveira Gino Bruno Francozo Al Qassimi Hospital Sharjah United Arab Emirates Innovatec’s-Biotechnology Research and Development S&atildeo Carlos Brazil Department of Physical Chemistry UNESP Araraquara Brazil Department of Chemistry USP FFCLRP Ribeir&atildeo Preto Brazil

Bacterial cellulose (BC) can be used in wide area of applied scientific, especially for tissue regeneration and regenerative medicine, lately, bacterial cellulose mats are used in the treatment of skin conditions such as burns and ulcers, because of the morphology of fibrous biopolymers serving as a support for cell proliferation, its pores allow gas exchange between the organism and the environment. Moreover, the nanostructure and morphological similarities with collagen make BC attractive for cell immobilization, cell support and Natural Extracellular Matrix (ECM) Scaffolds. In this scope, Natural ECM is the ideal biological scaffold since it contains all the components of the tissue. The development of mimicking biomaterials and hybrid biomaterial can further advance directed cellular differentiation without specific induction. The extracellular matrix (ECM) contains several signals that are received by cell surface receptors and contribute to cell adhesion and cell fate which control cellular activities such as proliferation, migration and differentiation. As such, regenerative medicine studies often rely on mimicking the natural ECM to promote the formation of new tissue by host cells, and characterization of natural ECM components is vital for the development of new biomimetic approaches. In this work, the bacterial cellulose fermentation process is modified by the addition of vegetal stem cell to the culture medium and natural materials before the bacteria are inoculated. In vivo behavior using natural ECM for regenerative medicine is presented.

关键词： Bacterial Cellulose (Nanoskin) Natural Nanocomposites Regenerative Medicine Stem Cells

The Basic Competences Can Be Developed Through the Dual Modality in a High Vulnerability Professional Technical License

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Journal of Philosophy Study 2021年第9期11卷 692-712页

作者： joséManuel Salum Tomé Catholic University of Temuco TemucoChile

In the context of professional technical education,dual modality has been a commitment of the Chilean Ministry of Education to improve professional technical training in high *** change has involved a curricular innovation around the development of basic competences where,in addition to the teacher and the traditional classroom,other agents and other scenarios that share the work of teaching *** this context,the research consigned,according to its objectives and methodological formulation,describes the characteristics of the dual professional technical training of the administration specialty of the Monseñor Guillermo carlos Hartl Educational Complex of the Pitrufquén Commune,in *** is intended to investigate the effectiveness of the integrated curriculum through the assessment of achievement of the basic competencies of students who are in the 4th middle year of said specialty(with dual mode),in direct relation to the required graduate and professional profile and in contrast,with that of students of the specialty of“electricity”,without the dual modality.

关键词： curriculum design curriculum basic skills didactics methodology

Kaphta:Text mining web tool to extract information on the anticancer activity of polyphenols

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Journal of Polyphenols 2022年第2期4卷 87-100页

作者： Ramon Gustavo Teodoro Marques da Silva Samuel Lucas Santos Gomes Paulo Muniz deÁvila Gustavo joséda Silva Ana Lucia Fachin Edilson carlos Caritá Mozart Marins Biotechnology Unit University of Ribeirão PretoAv.Costábile Romano 2201Ribeirão PretoSP 14096-900Brazil Federal Institute of Education Science and Technology of South of Minas Gerais–IFSULDEMINASPouso AlegreMG 37550-000Brazil Medicine School University of Ribeirão PretoRibeirão PretoSP 14096-900Brazil Centro de Ciências Exatas Naturais e TecnológicasUniversidade de Ribeirão PretoRibeirão Preto SP 14096-900Brazil

In this paper,we describe the application of Kaphta architecture,a resource for text mining of the anticancer activity of *** anticancer activity of these compounds against different types of cancer has been widely reported in the literature and they are one of the most promising molecules for the development of anticancer *** architecture,which comprises four sequential and well-defined steps,uses a hybrid approach composed of a dictionary,rules and machine learning to identify abstracts containing sentences with associations between polyphenol,cancer and gene *** application of the architecture on 23826 PubMed abstracts generated a knowledge base of indexed abstracts with 172169 sentences containing,polyphenol-cancer and polyphenol-gene associations.A Web tool was implemented that allowed the user to search for information on 2006 polyphenols,240 cancers and 3121 genes entities,and 11750 polyphenol-cancer and 9160 polyphenol-gene associations indexed in the knowledge base.A ranking algorithm calculates scores for each indexed abstract considering the number and type of sentences with entities and rules recognized.A test with users demonstrated that the visualization resources on the web tool contributes to the understanding of the association between polyphenols,genes and cancers,in comparison with the PubMed *** Kaphta architecture and web tool permits to extract knowledge on the anticancer activity of polyphenols and can thus contribute to the exploration of these molecules in the development of anticancer therapies.

关键词： polyphenols cancer text-mining

Beta-blocker therapy in elderly patients with renal dysfunction and heart failure

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Journal of Geriatric Cardiology 2021年第1期18卷 20-29页

作者： Juan Martínez-Milla Marcelino Cortés García Julia Anna Palfy Mikel Taibo Urquía Marta López Castillo Ana Devesa Arbiol Ana Lucía Rivero Monteagudo María Luisa Martín Mariscal Inés Jiménez-Varas Sem Briongos Figuero Juan Antonio Franco-Pelaéz joséTuñón Department of Cardiology Hospital Universitario Fundación Jiménez Díaz-Quirónsalud Centro Nacional de Investigaciones Cardiovasculares MadridSpain Department of Cardiology Hospital Central de Asturias Department of Cardiology Hospital Universitario Ruber-Quirónsalud Department of Cardiology Hospital Universitario Infanta Leonor Department of Endocrinology Hospital Clínico San Carlos Universidad Autónoma de Madrid CIBERCV MadridSpain

OBJECTIVE To assess the role of beta-blockers(BB)in patients with chronic kidney disease(CKD)aged≥75 *** AND RESULTS From January 2008 to July 2014,we included 390 consecutive patients≥75 years of age with ejection fraction≤35%and glomerular filtration rate(GFR)≤60 m L/min per 1.73 m^*** analyzed the relationship between treatment with BB and mortality or cardiovascular *** mean age of our population was 82.6±4.1 *** ejection fraction was 27.9%±6.5%.GFR was 60-45 m L/min per 1.73 m^2 in 50.3%of patients,45-30 m L/min per 1.73 m^2 in 37.4%,and<30 m L/min per 1.73 m^2 in 12.3%.At the conclusion of follow-up,67.4%of patients were receiving *** median follow-up was28.04(IR:19.41-36.67)*** the study period,211 patients(54.1%)died and 257(65.9%)had a major cardiovascular event(death or hospitalization for heart failure).BB use was significantly associated with a reduced risk of death(HR=0.51,95%CI:0.35-0.74;P<0.001).Patients receiving BB consistently showed a reduced risk of death across the different stages of CKD:stage IIIa(GFR=30-45 m L/min per 1.73 m^2;HR=0.47,95%CI:0.26-0.86,P<0.0001),stage IIIb(GFR 30-45 m L/min per 1.73 m^2;HR=0.55,95%CI:0.26-1.06,P=0.007),and stages IV and V(GFR<30 m L/min per 1.73 m~2;HR=0.29,95%CI:0.11-0.76;P=0.047).CONCLUSIONS The use of BB in elderly patients with HFr EF and renal impairment was associated with a better *** of BB should be encouraged when possible.

关键词： CKD GFR Beta-blocker therapy in elderly patients with renal dysfunction and heart failure

Land-use change and windstorms legacies drove the recolonization dynamics of laurel forests in Tenerife, Canary islands

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Forest Ecosystems 2023年第1期10卷 113-124页

作者： Vicente Rozas Maria A.Garcia-Lopez jose M.Olano Gabriel Sanguesa-Barreda Miguel Garcia-Hidalgo Susana Gomez-Gonzalez Roberto Lopez-Rubio jose M.Fernandez-Palacios Ignacio Garcia-Gonzalez Laura Lozano-Lopez Paula Garcia-Gonzalez Ana I.Garcia-Cervigon EiFAB-iuFOR Universidad de ValladolidCampus Duques de Soria42004SoriaSpain Departamento de Biologia-IVAGRO Universidad de CadizCampus Rio San Pedro11510Puerto RealSpain Center for Climate and Resilience Research(CR)2 Blanco Encalada 20028370449SantiagoChile Area de Biodiversidad y Conservacion Universidad Rey Juan Carlosc/Tulipan s/n28933MostolesSpain Grupo de Ecologia y Biogeografia Insular Universidad de La Laguna38206La LagunaTenerifeSpain BIOAPLIC Departamento de BotanicaEscola Politecnica Superior de EnxenariaCampus TerraUniversidade de Santiago de CompostelaLugo27002Spain

Laurel forests are quite relevant for biodiversity conservation and are among the island ecosystems most severely damaged by human *** the past,Canary laurel forests have been greatly altered by logging,livestock and *** remains of laurel forests are currently protected in the Canary Islands(Spain).However,we miss basic information needed for their restoration and adaptive management,such as tree longevity,growth potential and responsiveness to natural and anthropogenic *** dendrochronological methods,we studied how forest dynamic is related to land-use change and windstorms in two well-preserved laurel forests on Tenerife *** cores were collected from over 80 trees per stand at three stands per *** used ring-width series to estimate tree ages and calculate annual basal area increments(BAI),cumulative diameter increases,and changes indicative of released and suppressed *** tree species were found in all stands,with Laurus novocanariensis,Ilex canariensis and Morella faya being the most common *** some individuals were over 100 years old,61.8%-88.9% of the trees per stand established between 1940 and 1970,coinciding with a post-war period of land abandonment,rural exodus and the onset of a tourism *** trees have shown growth rates larger than 1 cm diameter per year and most species have had increasing BAI trends over the past *** growth releases occurred after windstorms at both sites,but the effects of windstorms were site-dependent,with the 1958 storm affecting mainly the eastern tip of the island(Anaga massif)and the 1991 storm the western tip(Teno massif).Given the great ability of laurel forest trees to establish after land use cessation and to increase growth after local disturbances such as windstorms,passive restoration may be sufficient to regenerate this habitat in currently degraded areas.

关键词： Canary islands Dendroecology Disturbance Forest structure Macaronesia Management cessation Tree rings

Inflammasome activation in decompensated liver cirrhosis

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World Journal of Hepatology 2016年第4期8卷 207-210页

作者： joséM González-Navajas FISABIO Biomedical Research Foundation Hospital General Universitario de Alicante03010 AlicanteSpain Networked Biomedical Research Center for Hepatic and Digestive Disease(CIBERehd) Instituto de Salud Carlos III28029 MadridSpain

Inflammation participates in the pathogenesis ofmany liver diseases, including liver cirrhosis. Certain inflammatory citokines, such as interleukin(IL)-1β and IL-18, are produced after the activation of a multiprotein complex known as the inflammasome. Activation of the inflammasome has been documented in several liver diseases, but its role in the development and progression of liver cirrhosis or the complications associated with this disease is still largely unknown. We have recently studied the impact of the inflammasome in the sterile inflammatory response that takes place in the ascitic fluid of patients with decompensated cirrhosis, providing evidence that activation of the absent in melanoma 2(AIM2) inflammasome is an important response in these patients. Ascitic fluid-derived macrophages were able to mount a very robust AIM2-mediated response even in the absence of a priming signal, which is usually required for the full activation of all the inflammasomes. In addition, high level of inflammasome activation in these patients was associated with a higher degree of liver disease and an increased incidence of spontaneous bacterial peritonitis. These results may help explain the exacerbated inflammatory response that usually occurs in patients with decompensated cirrhosis in the absence of detectable infections. Thus, inflammasomes should be considered as possible therapeutic targets in sterile inflammatory complications in patients with cirrhosis.

关键词： Cirrhosis Ascites Inflammasome Absentin melanoma 2 Interleukin-1β

Analysis of the Identification of Local Agriculture Products in the Municipality of Aileu, Manufahi, and Ainaro That Have Potential for Export and Attract Foreign Investments in Agricultural Sector

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巴西亚马孙阿马帕州境内两湖流域人发和环境样品中的汞

Agricultural Sciences（农业科学） 2023年第2期

作者： Vicente De Paulo Correia josé Nelson Salsinha Oscar Da Silva carlos Da Conceição De Deus Mateus De Jesus Gomes Graciano Soares Gomes Decio R. Sarmento

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AMBIO－人类环境杂志 1999年第4期28卷 296-301页

作者： Jean Remy Davée Guimar■es Anne-Hélène Fostier Maria Cristina Forti josé Adolfo Melfi Helena Kehrig Jane Beatriz Narvaez Mauro Olaf Malm josé Francisco Krug 李春红 PhD adjunct professor Lab. de Radioisótopos Instituto de Biofísica Carlos Chagas Filho Universidade Federal do Rio De Janeiro Bloco G CCS Ilha do Fundāo Rio de JaneiroCep 21949-900Brazil. PhD Research fellow Seco de Isótopos EstáveisCentro de Energia Nuclear na Agricultura(CENA/USP)Av. Centenàrio 303 Piracicaba SP Cep 13416-000 Brazil PhD researcher at Instituto Nacional de Pesquisas Espaciais(INPE) CP 515Sāo José dos CamposSāo Paulo Cep 12201-970 Brazil. PhD PhD fellow Lab. de Radioisótopos Instituto de Biofísica Carlos Chagas Filho Universidade Federal do Rio de Janeiro Bloco G CCS Ilha do Fundāo Rio de Janeiro Cep 21949-900 Brazil PhD fellow Lab. de Radioisótopos Instituto de Biofísica Carlos Chagas Filho Universidade Federal do Rio de Janeiro Bloco G CCS Ilha do Fundāo Rio de JaneiroCep 21949-900 Brazil PhD adjunct professor Lad. de Radioisótopos Instituto de Biofísica Carlos Chagas Filho Universidade Federal do Rio de JaneiroBIoco G CCS Ilha do Fundāo Rio de Janeiro Cep 21949-900. Brazil PhD researcher Secāo de Isótopos EstáveisCentro de Energia Nuclear na Agricultura (CENA/USP) Av. Centenário 303 Piracicaba SP Cep 13416-000 Brazil 黄河流域水环境监测中心

本文测定了巴西亚马孙阿马帕州两个相似湖泊中的沉积物、食鱼鱼以及湖区人发中的总汞量。这两个湖分别为:受采金矿区排放汞影响的Duas Boeas湖(DBL)和其北部15km处原始的Praeudba湖(PL)。在DBL沉积物中,纵剖面汞的含量范围为汞污染的发... 详细信息

本文测定了巴西亚马孙阿马帕州两个相似湖泊中的沉积物、食鱼鱼以及湖区人发中的总汞量。这两个湖分别为:受采金矿区排放汞影响的Duas Boeas湖(DBL)和其北部15km处原始的Praeudba湖(PL)。在DBL沉积物中,纵剖面汞的含量范围为汞污染的发展进程提供了清晰的证据,并且发现DBL区鱼体和人发中汞的含量远远高于PL区。沉积物中的^(203)Hg甲基化速率及鱼体中甲基汞的比率,都显示出汞在两湖中具有较高的生物有效性。当地成年人每天平均食鱼量约为200g,而在DBL湖,95%的鱼样(样品数N=40)超过15ng Hg/g这一安全限。Hg在人发中平均含量为28μg/g(N=15),已在一个可引发神经中毒效应的水平上。然而,在未受污染的PL湖,汞含量也较高,在这里67%～100%(N=34)鱼类的汞含量超过安全限,87%的人发样品(N=15)高于10ng Ha/g。建议这两个区域的儿童和孕妇应吃非食鱼性的鱼类和/或小鱼类,对阿马帕沿海平原的其他区域也应开展汞含量水平的调查。

关键词：巴亚亚马孙阿马帕州湖泊沉积物人发汞污染