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An optimized procedure for plant recovery from somatic embryos significantly facilitates the genetic improvement of Vitis

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Horticulture Research 2014年第1期1卷 183-189页

作者： Zhijian T Li Kyung-Hee Kim Sadanand A Dhekney jonathan R Jasinski Matthew R creech Dennis J Gray Grape Biotechnology core Laboratory Mid-Florida Research and Education CenterUniversity of Florida/IFASApopkaFL 32703-8504USA Department of Plant Sciences Sheridan Research and Extension CenterSheridanWY 82801USA

Plant regeneration from grapevine(Vitis spp.)via somatic embryogenesis typically is *** of plants from Vitis rotundifolia Michx.(muscadine grape)is particularly problematic due to extremely low efficiency,including extended culture durations required for embryo–plant *** plant recovery is an obstacle to the selection of improved genetically modified *** embryos(SEs)of *** cultivar Delicious(Del-HS)and Vitis vinifera L cultivar Thompson Seedless(TS)were used to identify culture media and conditions that promoted embryo differentiation and plant conversion;this resulted in a two-step culture *** comparative culture experiments,c2D medium containing 6%sucrose was the most effective,among four distinct formulae tested,for inducing precocious SE germination and cell *** medium,further supplemented with 4 mM 6-benzylaminopurine(c2D4B),was subsequently determined to enhance post-germinative growth of *** medium supplemented with 0.5 mM 1-naphthaleneacetic acid(MSN)was then utilized to stimulate root and shoot growth of germinated *** average of 35%and 80%‘Del-HS’and‘TS’SE,respectively,developed into *** plants developed robust root and shoot systems and exhibited excellent survival following transfer to *** 150 plants of‘Del-HS’were regenerated and established within 2.5 months,which is a dramatic reduction from the 6-to 12-month time period previously ***,88‘TS’plant lines were obtained within the same time ***,seven out of eight Vitis cultivars exhibited significantly increased plant conversion percentages,demonstrating broad application of the two-step culture system to produce the large numbers of independent plant lines needed for selection of desired traits.

关键词： cultivar Vitis culture

基因-病毒治疗系统cNHK300-murine endostatin治疗肝癌的实验研究

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中华医学杂志 2004年第11期84卷 943-948页

作者：李根丛聂明明杨甲梅苏长青孙立臣钱炎珍 jonathan Sham 方国恩吴孟超钱其军第二军医大学附属东方肝胆外科医院病毒和基因治疗实验室上海200438 第二军医大学附属长海医院普外科第二军医大学附属东方肝胆外科医院肝胆外科上海200438 香港大学临床肿瘤系

目的　探讨一种新的基因病毒治疗系统cNHK30 0 murineendostatin(简称cNHK30 0 mE)对肝癌的治疗作用。方法　利用基因重组技术构建一种用人端粒酶逆转录酶 (hTERT)启动子控制腺病毒E1A基因表达并携带内皮抑素基因的基因病毒治疗系... 详细信息

目的　探讨一种新的基因病毒治疗系统cNHK30 0 murineendostatin(简称cNHK30 0 mE)对肝癌的治疗作用。方法　利用基因重组技术构建一种用人端粒酶逆转录酶 (hTERT)启动子控制腺病毒E1A基因表达并携带内皮抑素基因的基因病毒治疗系统 ;通过 5 0 %组织培养感染剂量 (TcID5 0 )方法和细胞活性检测法四氮唑盐比色试验 (MTT法 )观察cNHK30 0 mE在 2种肝癌细胞株 (HepGII及Hep3B)及 1株正常的成纤维细胞株 (MRc 5 )中的病毒增殖能力和对细胞的杀灭能力 ;通过肝癌SMMc772 1细胞裸鼠皮下移植瘤模型观察该病毒对肝癌生长和对肿瘤血管生成的抑制作用 ;利用Western印迹法和酶联免疫吸附实验 (ELISA)法检测内皮抑素基因在体内和体外的表达。结果分别感染肝癌细胞株和正常细胞株 96h后病毒的增殖倍数相差 32 96倍 ,其达到半数杀伤量 (ED50 )时的感染复数 (MOI)值相差 2 5倍 ,差异有显著意义 ,且两者均明显优于ONYX 0 15 ;cNHK30 0 mE能明显抑制肝癌皮下移植瘤内血管的生成 ,对肿瘤生长的抑制作用与携带同一治疗基因的非增殖型腺病毒和不携带治疗基因的增殖型腺病毒ONYX 0 15相比均增强 (P均 <0 0 1) ;其所介导的内皮抑素基因在体内和体外的表达量均与携带该基因的非增殖型腺病毒载体相比均增高 (P <0 0

关键词：基因-病毒治疗系统 cNHK300-murine endostatin 肝癌端粒酶逆转录酶 hTERT 内皮抑素

Assessing change of Lamto Reserve Area Based on the MODIS Time Series Data and Bioclimatic Factors Using BFAST Algorithms

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American Journal of Plant Sciences 2022年第4期13卷 517-540页

作者： christian jonathan Anoma Kouassi Dilawar Khan Lutumba Suika Achille James Kehinde Omifolaji Mikouendanandi Mouendo Rahmat Brice Espoire Kebin Zhang Xiaohui Yang School of Soil and Water conservation Beijing Forestry University Beijing China School of Ecology and Nature conservation Beijing Forestry University Beijing China Department of Forestry and Wildlife Management Federal University Dutse Dutse Jigawa State Nigeria School of Economics and Management Beijing Forestry University Beijing China Institute of Desertification Studies Chinese Academy of Forestry Beijing China

Lamto Reserve area is a savannah landscape threatened by periodic drought, and anthropogenic activities leading to natural ecological imbalance. The ecological support services of the landscape had been significantly impacted by the grassland ecosystem. The Breaks for Additive Season and Trend Algorithms have been implemented in R to analyze the land cover/land use dynamic in relation to the climatic driver of Lamto forest from 2000 to 2020. We examine the vegetation state breaks using vegetation phenological patterns, and several time series including the Normalized Difference Vegetation Index and the Enhanced Vegetation Index, were studied utilizing Breaks for Additive Season and Trend. The findings indicate that the phenological changes in the vegetation in 2020 resulted from an increased temperature from (27.7°c) to (32.17°c), and a decrease in precipitation (71.75 millimeters). The analysis of variance ANOVA of the non-parametric Mann-Kendall test reveals a strong correlation between Precipitation/Evapotranspiration Grass (p mperature/Evapotranspiration Grass (p ce Vegetation Index (p in vegetation detected by the Breaks for Additive Season and Trend Algorithms were caused by temperature extremes and reduced rainfall.

关键词： Assessing Break change Detection Land cover climate Extreme Vegetation Vulnerability

冠状动脉内影像学临床应用专家共识(第一部分):对冠状动脉介入治疗的指导与优化

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中华心血管病杂志 2019年第1期47卷 5-25页

作者： Lorenz Raber Gary S Mintz Konstantinos c Koskinas Thomas W Johnson Niels R Holm Yoshinubo Onuma Maria D Radu Michael Joner Bo Yu Haibo Jia Nicolas Meneveau Jose M.de la Torre Hemandez Javier Escaned jonathan Hill Francesco Prati Antonio colombo carlo di Mario Evelyn Regar Davide capodanno William Wijns Robert A Byme Giulio Guagliumi 瑞士伯尔尼大学医院心内科法国让·明乔兹大学医院心内科法国勃艮第弗朗什孔德大学西班牙瓦尔德里亚马克大学医院心内科西班牙圣卡洛斯临床医院和康普顿斯大学英国国王学院医院心内科意大利圣乔万尼医院心内科意大利圣拉斐尔科学研究所介入科、心胸血管科意大利卡雷奇大学医院结构介入心脏病学瑞士苏黎世大学医院心血管外科意大利卡塔尼亚大学路得里医院心胸血管和移植科、维托里奥诊所美国纽约心血管研究基金会爱尔兰萨尔塔大学医疗集团、爱尔兰戈尔韦国立大学兰贝转化医学和康复研究意大利教皇约翰二十三世医院心内科英国布里斯托尔心脏研究所、布里斯托尔大学医院国民健康基金会信托基金丹麦奥胡斯大学医院心内科荷兰鹿特丹伊拉斯姆斯大学医疗中心心脏介入科、胸部中心丹麦哥本哈根大学国王医院心脏中心德国慕尼黑技术大学德国慕尼黑心脏中心德国慕尼黑心血管研究中心、合作网站慕尼黑心脏联盟中国哈尔滨医科大学附属第二医院心内科、教育部心肌缺血重点实验室

欧洲心血管介入协会(EAPcI)专家组系统总结了血管内超声(IVUS)和光学相干断层成像(OcT)这两种血管内影像学检查临床应用指征的现有证据,提供了关于IVUS和OcT指导经皮冠状动脉介入治疗(PcI)的应用价值,并明确了最可能从腔内影像学指导的... 详细信息

欧洲心血管介入协会(EAPcI)专家组系统总结了血管内超声(IVUS)和光学相干断层成像(OcT)这两种血管内影像学检查临床应用指征的现有证据,提供了关于IVUS和OcT指导经皮冠状动脉介入治疗(PcI)的应用价值,并明确了最可能从腔内影像学指导的介入治疗中获得临床收益的患者或病变类型,同时详细论述了PcI前如何使用IVUS或OcT优化支架尺寸(支架长度和直径)和手术策略的选择。此外,专家推荐对支架失败(支架内再狭窄或支架内血栓形成)的患者应常规进行冠状动脉内影像学检查,并首选OcT。最后,重点论述了IVUS和OcT在指导PcI和评估支架失败两个方面的优势和局限性,并对未来需要深入研究的领域进行了展望。

关键词：冠状动脉疾病超声检查介入性体层摄影术光学相干经皮冠状动脉介入治疗

Factors Associated with Echocardiographic Abnormalities in Patients with chronic Kidney Disease in a Tertiary Hospital in Ivory coast

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Open Journal of Nephrology 2022年第1期12卷 36-47页

作者： Weu M. Tia Abdoulaye Togo Manzan A. Wognin Reine M. Koffi jonathan Kpan Bourhaima Ouattara Dame A. Gnionsahe Department of Internal Medicine University Teaching Hospital (CHU) of Bouaké Bouaké C&ocirc te d'Ivoire Nephrology-Hemodialysis Department University Teaching Hospital (CHU) of Yopougon Abidjan C&ocirc te d'Ivoire

Background: cardiovascular risk factors (cVRF) are very frequent in pa-tients with chronic kidney disease (cKD) and impose a new environment to which the heart must adapt. cardiac ultrasound is a non-invasive and easy-to-perform examination that allows quantitative and qualitative assessment of the anatomy and function of the heart. The objectives of this study were to describe abnormalities observed on trans-thoracic Doppler-echocar-diography and to investigate the factors associated with them. Materials and Method: This was a monocentric retrospective cross-sectional study conducted in cKD patients hospitalized in a hospital center in côte d'Ivoire from January 2017 to December 2018. Results: One hundred and four cases were collected with a mean age of 48.87 ± 14.47 years and a sex ratio of 1.7. Patients with end-stage-renal-disease (ESRD) represented 83.7% with 55.8% of cases of chronic glomerulonephritis. cardiovascular risk factors were 100% anemia, 84.6% inflammatory profile, 77.9% hypertension, 76.9% hypocalcemia and in 67.3% oedema. cardiac abnormalities were observed in 78.8% of patients. Left ventricular hypertrophy (LVH), accounting for 20.2% of cases, was associated with male gender (OR 0.127 cI 0.025 - 0.643;p = 0.013) and hypertensive nephropathy (OR 0.189 cI 0.056 - 0.637;p = 0.007). History of hypertension (OR 0.297 cI 0.084 - 1.050;p = 0.060) and diabetes (OR 5.315 cI 1.260 - 22.419;p = 0.023), hypertensive nephropathy (OR 0.174 cI 0.052 - 0.585;p = 0.005) and hypocalcemia (OR 6.094 cI 1.723 - 21.559;p = 0.005) are incriminated in the development of left ventricular dilatation (LVD) which accounted for 38.5% of cases. conclusion: Left ventricular hypertrophy and dilatation are the main echocardiographic abnormalities observed in our population.

关键词： chronic Renal Failure Left Ventricular Hypertrophy Ivory coast

社区专业姑息治疗团队对患者临终时住院、急诊以及选择在医院内死亡的影响：一项汇总分析

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英国医学杂志中文版 2015年第10期18卷 587-593页

作者： Hsien Seow associate professor Kevin Brazil professor of palliative care jonathan Sussman associate professor Jose Pereira head of division of paUiative medicine Denise Marshall associate professor Peter c Austin professor Amna Husain associate professor Jagadish Rangrej senior biostattsttctan Lsa Barbera associate professor 饶洁于世英 Department of Oncology McMaster University HamiltonOntario LSV 5C2 Canada School of Nursing and Midwifery Queen's University BelfastBelfast UK Division of Palliative care Faculty of Medicine University ofOttawa Ottawa Ontario Canada Department of Family Medicine McMaster UniversityHamilton Ontario Canada Institute for Health Policy Management and Evaluation DallaLana School of Public Health University of Toronto TorontoOntario Canada Department of Family and community Medicine University ofToronto Toronto Ontario Canada institute for clinical Evaluative Sciences Toronto OntarioCanada Department of Radiation Oncology Institute of Health PolicyManagement and Evaluation University of Toronto TorontoOntario Canada 华中科技大学同济医学院附属同济医院

目的汇总11个开展家庭医疗服务的专业姑息治疗团队效应。设计进行一项回顾性队列研究的汇总分析。地点加拿大，安大略省。参与者3109名在2009-2011年接受专业姑息治疗团队医疗服务的患者（暴露组）及3109名在此期间接受常规医疗服务的... 详细信息

目的汇总11个开展家庭医疗服务的专业姑息治疗团队效应。设计进行一项回顾性队列研究的汇总分析。地点加拿大，安大略省。参与者3109名在2009-2011年接受专业姑息治疗团队医疗服务的患者（暴露组）及3109名在此期间接受常规医疗服务的患者（非暴露组）。干预措施虽然这些姑息治疗团队所在地区和规模不同，但包含了相同的核心团队成员和角色分配，即能够为患者在其家中提供综合姑息治疗的姑息治疗专科医师、护士和家庭医生，团队任务包括全天候的管理症状、给予医疗信息和治疗措施以及协调服务。主要结局测量指标患者（a）在死亡前最后2周在医院度过；（b）死亡前最后2周有过急诊；（c）在医院内死亡。结果暴露组和非暴露组中癌症患者约占80％，且78％患者接受临终家庭医疗护理服务的平均时间相同。在所有姑息治疗团队中，暴露组中分别有970（31．2％）和896（28．9％）名患者在生命最后2周住院治疗和有急诊经历，而在非暴露组，分别为1219（39．3％）和1070（34．5％）名患者（P〈0．001）。暴露组与非暴露组患者在生命终期住院和经历急诊的汇总相对危险度分别为0．68[95％可信区间（cI，0．61～0．76）]和0．77[95％cI（0．69～0．86）]。与非暴露组相比，暴露组患者选择院内死亡的更少[503（16．2％）比887（28．6％），P〈0．01]，且在医院内濒死的汇总相对危险度为0．46（0．40-0．52）。结论尽管社区专业姑息治疗团队的组成和地区不同，但均能有效降低患者临终时急诊及选择在医院内死亡比例。

关键词：社区生命死亡医院急诊组对专家治疗

Trends in Archaea community diversity along a soil moisture gradient in the Tibetan Plateau

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A Study of Zoledronic Acid as Neo-Adjuvant, Perioperative Therapy in Patients with Resectable Pancreatic Ductal Adenocarcinoma

第八次全国土壤生物与生物化学学术研讨会暨第三次全国土壤健康学术研讨会

作者： Yu Shi jonathan M.Adams Yingying Ni Xin Jing Jin-Sheng He Haiyan chu State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of Sciences Department of Biological Sciences Seoul National University Department of Ecology College of Urban and Environmental Sciences and Key Laboratory for Earth Surface Processes of the Ministry of EducationPeking University

There has been little study of the biogeography of soil archaeal communities on a regional *** a previous study,we found that variation in bacterial communities on the Tibetan Plateau was strongly determined by soil c:N ***,using an 16 SrRNA archaeal primer,we characterized the archaeal communities of 94 Tibetan soil samples scattered across an area of 1,000,000 *** results showed that Thaumarchaeota(79.27%) and Halobacteria(8.75%) were the dominant phyla across all the soils,together accounting for at least 88%of the archaeal reads in every *** terms of alpha diversity,OTU and phylogenetic diversity(PD) were both negatively correlated with soil moisture(r=-0.47,P ctively),and soil moisture also had a significant influence on soil archaeal community *** distance,a potential measure of the legacy effect of evolutionary and dispersal factors,was less important than measured environmental factors in determining the broad scale archaeal community pattern across the Tibetan *** close correlation was encountered between soil bacterial diversity and archaeal *** findings provide new insights into the ecology of soil archaea in semi-arid environments,for example the strong influence of soil moisture on archaeal community composition and *** correlations may allow better prediction of future responses of soil archaea to environmental change.

关键词： Biogeography Tibetan Plateau Archaeal community Phylogenetic diversity

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Journal of cancer Therapy 2013年第3期4卷 797-803页

作者： Dominic E. Sanford Matthew R. Porembka Roheena Z. Panni jonathan B. Mitchem Brian A. Belt Stacey M. Plambeck-Suess Goldie Lin David G. DeNardo Ryan c. Fields William G. Hawkins Steven M. Strasberg craig Lockhart Andrea Wang-Gillam Simon Peter Goedegebuure David c. Linehan Alvin J. Siteman cancer center St. Louis USA Department of Medicine School of Medicine Washington University St. Louis USA Department of Surgery School of Medicine Washington University St. Louis USA

Background: Pancreatic ductal adenocarcinoma (PDAc) is an aggressive malignancy characterized by abundant granulocytic myeloid-derived suppressor cells (G-MDSc = cD45+/Lin﹣/cD33+/cD11b+/cD15+), which infiltrate tumors and suppress anti-tumor immunity. We have previously demonstrated in a murine model of PDAc that zoledronic acid (ZA) depletes G-MDSc resulting in decreased tumor growth and improved survival. We report here the results of a phase 1 clinical trial (NcT00892242) using ZA as neo-adjuvant, perioperative therapy in patients with non-metastatic, resectable pancreatic adenocarcinoma. Methods: Eligible PDAc patients received ZA (4 mg) IV 2 weeks prior to surgery. Patients then received 2 additional doses of ZA 4 weeks apart. Blood and bone marrow were obtained from patients prior to treatment with ZA and 3 months after surgery for analysis of G-MDSc by flow cytometry. Results: Twenty-three patients received pre-operative ZA with at least 6 months of follow-up. Only 15 PDAc patients had nonmetastatic PDAc, which was amenable to resection. ZA was well tolerated, and all adverse events were grade 1 or 2. The most common adverse events were fatigue, abdominal pain/discomfort, anorexia, and arthralgia. Of resected PDAc patients treated with ZA, 1- and 2-year overall survival (OS) was 85.7% and 33.3%, respectively, with a median OS of 18 months. This group had a 1- and 2-year progression-free survival (PFS) of 26.9% and 8.9%, respectively, with a median PFS of 12 months. The prevalence of G-MDSc was unchanged in the blood and bone marrow of PDAc patients pre- and post-treatment with ZA. conclusion: ZA is safe and well tolerated as neo-adjuvant, peri-operative therapy in PDAc patients. In this small study, we did not observe a difference in OS or PFS compared to historical controls. Also, there was no difference in the prevalence of G-MDSc in the blood and bone marrow of PDAc patients pre- and post-treatment with ZA.

关键词： Pancreatic cancer Zoledronic Acid Myeloid-Derived Suppressor cells

我国人类免疫缺陷病毒-1主要流行株外膜蛋白基因V3-V4区及其临近区域的特征性氨基酸分析

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中华医学杂志 2005年第13期85卷 897-902页

作者：梁浩邢辉 jonathan Z.Li 魏民洪坤学冯毅赵全壁陈建平全宇滕涛邵一鸣中国疾病预防控制中心艾滋病/性病预防与控制中心 Department of Medicine University of CaliforniaSanFranciscoUSA

目的鉴定我国HIV-1主要流行毒株亚型的envV3-V4区及其临近区域的特征性氨基酸,并阐明其在追踪传染源和研究疫苗中的作用。方法应用nested-PcR对157份来自我国12个省份的HIV-1毒株env区序列进行扩增,并使用ABI377型测序仪测序,然后应用BL... 详细信息

目的鉴定我国HIV-1主要流行毒株亚型的envV3-V4区及其临近区域的特征性氨基酸,并阐明其在追踪传染源和研究疫苗中的作用。方法应用nested-PcR对157份来自我国12个省份的HIV-1毒株env区序列进行扩增,并使用ABI377型测序仪测序,然后应用BLAST、GcG、MEGA和VESPA等生物学软件或程序对env基因V3-V4区及其临近区域序列进行基因型鉴定、系统树分析及特征性氨基酸鉴定。结果157份样本包括54份B′(34·40%)、61份B′/c(38·85%)和42份cRF01-AE(26·75%)毒株。系统树分析结果显示,B′亚型毒株序列均与B·cN·RL42十分接近,B′/c毒株主要与97cN54A和97cNGX6F聚成一簇。而cRF01-AE序列与THcM240和97cNGX2F聚在一起,而且分别聚成明显不同的两个亚组。特征性氨基酸分析发现,我国B′和B′/c毒株分别具有8个保守的特征性氨基酸,而且与代表株的相同位点氨基酸基本一致。而cRF01-AE重组毒株具有11个保守的特征性氨基酸,其中有9个位点与97cNGX2F和TH·cM240不一致。包括这9个特征性氨基酸的样本主要来自除云南省以外的其他省份。结论目前流行于我国的B′和B′/c毒株具有单一的共同传染源,而cRF01-AE毒株可能是通过不同输入源或不同传播途径先后从泰国传入我国的。这将对我国艾滋病防治策略的制定和正在进行的疫苗研究具有重要的指导意义。

关键词：人类免疫缺陷病毒-1 氨基酸分析特征性外膜蛋白基因流行株 HIV-1毒株 nested 基因型鉴定 env基因流行毒株方法应用分析结果亚型毒株传播途径指导意义疫苗研究防治策略传染源系统树序列 PcR GcG 生物学分析及