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Tailoring the partitioning of proteins using ionic liquids as adjuvants in polymer-polymer aqueous biphasic systems

Green Chemical Engineering 2022年第4期3卷 328-337页

作者： Flavia F.Magalhaes Matheus M.pereira Rita de Cassia Superbi de Sousa Ana p.M.Tavares joao a.p.coutinho Mara G.Freire CICECO-Aveiro Institute of Materials Department of ChemistryUniversity of AveiroAveiro3810-193Portugal Separation process Laboratory(LpS) Department of Food TechnologyFederal University of Viçosa(UFV)P.H.Rolfs Av.s/n36570-000 ViçosaMGBrazil

Aqueous biphasic systems(ABS)are promising for proteins purification;however,when dealing with samples comprising several proteins,the selectivity towards a target protein is difficult to *** this work,the addition of ionic liquids(ILs)as adjuvants(5 wt%and 10 wt%)in ABS composed of polyethylene glycol(pEG)and dextran was investigated to tailor proteins partitioning between the coexisting *** liquid-liquid phase diagrams were determined at 298 K,followed by partition studies of three proteins(bovine serum albumin(BSA),immunoglobulin G(IgG),and cytochrome C(Cyt C)).partition coefficients of IgG and BSA indicate the preference of both proteins to the dextran-rich phase,whereas Cyt C has no preferential partitioning between the *** addition of chloride-based ILs as adjuvants allows to increase or decrease the partition coefficients,thus tailoring the proteins partitioning between the *** partitioning essentially depends on the IL content in each phase,whereas Cyt C and IgG partitioning is ruled by the ILs chemical structure and established *** docking was carried out to address the ILs effect on the proteins partitioning,supporting experimental observations,while identifying the specific interactions *** partition of each protein in polymer-salt ABS with ILs as adjuvants was determined,demonstrating the higher tailoring ability of polymer-polymer ABS when adding ***,the partition of each protein in presence of the remaining ones was determined,as well as the selectivity of the studied systems to separate each pair of proteins,paving the way for their use in liquidliquid chromatography.

关键词： Aqueous biphasic systems polymer-polymer systems protein partition Ionic liquids Adjuvants

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Nutrient content affecting Spodoptera frugiperda and Dalbulus maidis occurrence in corn

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Insect Science 2007年第2期14卷 117-123页

作者： CRISTINA SCHETINO BASTOS joao CARLOS CARDOSO GALVAO MARCELO coutinho pICANCO pAULO ROBERTO GOMES pEREIRA pAULO ROBERTO CECON EMBRApA/Algodao Centenário Campina Grande PB Brazil Departamento de Fitotecnia Universidade Federal de Vicosa (UFV) Vicosa MG Brazil. Departamento de Biologia Animal Universidade Federal de Vicosa (UFV) Vicosa MG Brazil. Departamento de Informática Campus Universitário Universidade Federal de Vicosa (UFV) Vicosa MG Brazil.

This work aims to study the effect of the nutrient contents in the leaves of corn on the incidence of Spodopterafrugiperda （Smith）（Lepidoptera： Noctuidae） and Dalbulus maidis （Delong ＆ Wolcott）（Homoptera： Cicadellidae）. The treatments were represented by unfertilized corn plants and corn plants fertilized with 250 kg of the formulation 4-14-8 （N- p-K）＋ 100 kg of ammonium sulfate （AS）/ha and 500 kg of the formulation 4-14-8 （N-p-K）＋200 kg of AS/ha, arranged in randomized blocks with four replicates. Attacks by S. frugiperda and D. maidis were higher as the nitrogen, sulfur, calcium and copper content in the leaves of corn increased. The effects of calcium and copper found in this study were not consistent with that of lower susceptibility associated with higher levels of these nutrients mentioned in the literature. We believe that there was a cumulative effect of the nutrients altogether, building up a nutritiously better vegetable which in turn had less reserve for defense, or that the content of both nutrients in the leaves were not high enough to induce such effects.

关键词： calcium copper corn cicada fall armyworm fertilization nitrogen sulfur

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Hyperacusis and stapes surgery:An observation in fifty patients after stapedotomy

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Journal of Otology 2021年第1期16卷 18-21页

作者： Mariline Santos Angela Reis Rego joao Lino Miguel coutinho Cecília Almeida Sousa Department of Otolaryngology Head and Neck SurgeryCentro Hospitalar Universitario do PortoPortugal

Objective:To assess hyperacusis after stapedotomy and its possible influencing *** design:prospective,interventional,and longitudinal ***:A tertiary referral ***:Fifty consecutive patients(35 females,mean age=46.8 years).Intervention:All patients underwent *** validated portuguese version of the“Hyperacusis Questionnaire”(HQ)was administered before and two weeks and one month after ***:No hyperacusis was reported by any patient before *** two weeks after surgery,all patients experienced hyperacusis,with a mean HQ at 16.88±6.54(range 4e25).One month after surgery,hyperacusis had already resolved in most ***,preoperative presentation or surgeon had no influence on HQ scores(p>0.05).patients with previous contralateral stapedotomy showed lower HQ scores(p=0.001).Audiological parameters improvement measured at one month after surgery(pTA,SRT and contralateral SRT)were associated with HQ higher ***:This study confirms that hyperacusis is a common complaint after stapedotomy that usually resolves in one month after *** HQ highest scores were registered among patients with the highest audiological gain after *** suggests that hyperacusis may be a positive prognostic factor for audiological success after stapedotomy.

关键词： Hyperacusis Stapes Otosclerosis Middle ear Surgery

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primary Liver Large B-Cell Lymphoma: A Rare Diagnosis

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Open Journal of Blood Diseases 2015年第1期5卷 9-12页

作者： Joana de Castro Rocha Raquel Lopes Ana Rita Cruz Cristina Goncalves joao Araujo Correia Jorge coutinho Internal Medicine Department Centro Hospitalar do PortoPortoPortugal Hematology Department Centro Hospitalar do PortoPortoPortugal

primary liver lymphoma is extremely rare, in most of cases it is a B cell lymphoma. Usually the diagnosis is made in middle-aged individuals and most of them have a relatively short life expectancy. In this article, the authors present a case report of a 75-year-old woman with symptoms of three weeks of evolution of diffuse abdominal pain, asthenia and anorexia. The analysis showed cyto cholestasis and the radiological image with lush hepatomegaly because of a large hepatic mass. The patient started chemotherapy and actually is well twenty months after the end of the chemotherapy.

关键词： Large B Cell Lymphoma Liver Rarity

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Delirium in hospitalized patients: Cohort observational in infirmary of medical clinic

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Open Journal of preventive Medicine 2012年第2期2卷 190-195页

作者： Rilva Lopes de Sousa-Munoz Leila coutinho Taguchi Mara Rufino de Andrade Katyara Mylena Silveira Ribeiro Lima Federal University of paraiba(UFpB) Joao PessoaBrazil Lauro Wanderley University Hospital(LWUH) Joao PessoaBrazil

Objectives: To determine prevalence, incidence and risk factors of delirium in hospitalized patients in the infirmary of medical clinic of the Lauro Wanderley University Hospital. Method: An observational cohort study evaluating 140 patients over 18 years between admission and discharge. The survey instrument was the Confusion Assessment Method. Results: The prevalence of delirium was 5.7% (8/140) and the incidence, 2.1%. In patients with delirium were observed alteration of sleep-wake cycle (6/75%), altered level of consciousness (6/75%), disorganized thinking (5/62, 5%) and psychomotor retardation (5/62 5%). There was a significant association of delirium and age, mechanical restraint in bed, prior history of cognitive impairment, auditory and visual sensory deficit, psychiatric hospitalization, poor health status and mortality. Conclusions: The prevalence of delirium at admission was lower than that found in other studies, but it was a predictor factor of hospital mortality.

关键词： Delirium Hospitalization Risk Factors Mortality

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production of Lettuce Seedlings with Association of Diazotrophic Bacteria and Humic Acids

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American Journal of plant Sciences 2018年第5期9卷 946-957页

作者： Carlos Augusto Rocha de Moraes Rego Bruna penha Costa Marinez Carpiski Sampaio Marcos Massuo Kashiwaqui joao Henrique Silva Caetano Vandeir Francisco Guimaraes Juan López de Herrera pablo Wenderson Ribeiro coutinho Cláudio Yuji Tsutsumi Maria Soraia Fortado Vera Cruz Thatiane Nepomuceno Alves Shirlene Souza Oliveira Ana Carolina pinguelli Ristau Hannah Braz Center of Agrarian Sciences State University of Western ParanáMarechal Candido RondonBrazil University School of Agricultural Technical Engineering Polytechnic University of MadridMadridSpain

The objective of this work was to verify the responses in agronomic and physiological characters of lettuce seedlings when submitted to different doses of humic acids and the presence or absence of inoculation of the bacterium Azospirillum brasilense. The experiment was carried out at the protected Cultivation Station and Biological Control Teacher Mário César Lopes, belonging to the Experimental Stations Nucleus of the State University of the West of paraná. The experimental design was a randomized block design, in a 5 × 2 factorial scheme, with four repetitions, totaling 40 plots. The first factor consisted of five solutions containing humic acid (100%, 75%, 50%, 25% and 0%) and the second factor was the presence or absence of the diazotrophic bacterium Azospirillum brasilense obtained by commercial product. Germination tests were carried out in addition to the determination of agronomic characters such as the number of fully expanded leaves, plant height, stem diameter, main root length, shoot dry mass, root dry mass, total dry mass, and Dickson quality index. The association of diazotrophic bacteria and humic acids did not influence the emergency rate, mean emergence time, mean speed of emergency, emergence speed index, plant height, and root length of lettuce seedlings. The dilutions of humic acids, regardless of inoculation with bacteria diazotrophic favored the development of the number of leaves, stem diameter and Dickson quality index. Inoculation of lettuce seeds with diazotrophic bacteria associated with the addition of humic acids, favored the development of the dry mass of root and total dry matter.

关键词： Azospirillum brasilense Humic Substances Lactuca sativa L. productive Characteristics

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Antibody-Cytokeratin Marker 34βE12 in prostate Cancer Detection

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Journal of pharmacy and pharmacology 2019年第4期7卷 187-197页

作者： Luiz Carlos de Araújo Souza Vinícius Carvalhêdo Cunha Hugo Oliveira de Figueiredo Cavalcanti joao Ricardo Alves Sandra Lúcia Branco Mendes coutinho Undergraduates of Medicine in the University Center of Brasilia(UniCEUB)and Researchers in the Department of Cytopathology and pathological Anatomy of the Base Institute of the Federal District(NUCAp-IHBDF) Hospital Medical Sector Sul-Asa SulBrasilia 70330-150Brazil Resident physician in Anatomical pathology of the Federal District Base Institute(NUCAp-IHBDF) Hospital Medical Sector SulAsa SulBrasilia 70330-150Brazil Urologist physician of the Clinical Body of the Federal District Base Institute and Urocentro(IHBDF) Hospital Medical Sector Sul-Asa SulBrasilia 70330-150Brazil physician Anatomopathologist and Head of the Department of Cytopathology and Anatomical pathology of the Federal District Base Institute(NUCAp-IHBDF) Hospital Medical Sector Sul-Asa SulBrasilia 70330-150Brazil

Introduction:The histological diagnosis of prostate cancer is commonly based on morphological *** presence of malignant tissue mixed with benign tissue,or the presence of carcinoma that mimics benignity may generate difficulty in the diagnostic ***,the application of immunohistochemistry contributes its diagnostic ***:To evaluate the 34βE12 marker in the detection of adenocarcinoma(ADn),atypical small acinar proliferation(ASAp),regular prostatic tissue(RpT)and regular prostatic tissue alternated by atrophy spotlights(RpTa)in transrectal biopsy guided by ultrasonography of patients with suspected prostate ***:Analysis of 34 patients who underwent ultrasound-guided transrectal biopsy with subsequent analysis by H&E staining and 34βE12 labeling for elucidation of neoplasms or diseased tissues with doubtful ***:The marker 34βE12 showed negativity in 100%of the neoplasms ADn,positivity in 100%of the benign prostatic tissues(RpT and RpTa);the patients with ASAp presented positivity(20%)and negativity(80%).The chi-square test(χ2)showed that there is an association(χ2=29.55 and pps,that is,the 34βE12 marker has a significant value(ppatients with prostatic neoplasia and benign prostatic *** and Conclusion:With the early screening of prostate cancer in the modern era,pathologists have become increasingly challenged to diagnose small outbreaks of cancer when only a few atypical glands are present in transrectal biopsy-guided *** 34βE12 marker becomes an important tool in elucidating diagnoses such as ADn and ASAp.

关键词： Antibody-cytokeratin marker 34βE12 adenocarcinoma(ADn) atypical small acinar proliferation(ASAp) regular prostatic tissue(RpT) regular prostatic tissue alternated by atrophy spotlights(RpTa)

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Diagnostic Value between 1984 and 2018 of Transrectal Biopsy Guided by Ultrasonography after Radical prostatectomy

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Journal of pharmacy and pharmacology 2019年第8期7卷 459-472页

作者： Luiz Carlos de Araújo Souza Vinícius Carvalhêdo Cunha Hugo Oliveira de Figueiredo Cavalcanti joao Ricardo Alves Grimar de Oliveira paula Sandra Lúcia Branco Mendes coutinho Undergraduates of Medicine in the University Center of Brasilia (UniCEUB) physician Anatomopathologist by Hospital Base of the Federal District (HBDF) physician Urologist of the Clinical Body of the Hospital Base of the District Federal and Urocentro (HBDF) physician Urologist by Hospital Base of the Federal District (HBDF) physician Anatomopathologist and Head of the Department of Cytopathology and Anatomical pathology of the Hospital Base of the Federal District (NUCAp-HBDF)

Objectives—to determine correlation between GSs(Gleason scores)on needle biopsy and Rp(radical prostatectomy),evaluating diagnostic tests on biopsy and Rp within the last years,between 1984 and ***—analysis of 100 patients,diagnosed with pCa(prostate cancer)needle biopsy using 18-gauge needle,who underwent Rp with lymphadenectomy and for which preoperative and postoperative GSs were *** group analysis used three categorization schemes for differentiation:mild,moderate and poor for the whole group and we determined SE(sensitivity),Sp(specificity),pVpR(positive predictive value),negative predictive value and ***—we found that 42%of the patients had no changes between GS on biopsy and prostatectomy,while 20%were overgraded and 38%undergraded by needle *** of+1 point in GS(32%)or-1 point(17%)was the most *** patients were classified as moderately differentiated by biopsies(78 and 35%in scheme 1 and 2 or 3,respectively),while 43%of patients received an intermediate differentiation *** accuracy varied from 44 to 76%for the analysis of all three ***—there are differences in correlation between GS on biopsy and on surgical specimen,and Gleason’s graduation also depends on the experience of the *** have shown that sextant biopsies using 18-gauge and a same group of pathologists showed acceptable concordance values(42%)between the GS on biopsy and prostatectomy.

关键词： pCa transrectal biopsy guided by ultrasonography Rp pSA(prostate specific antigen)

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