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World journal of Clinical Cases 2023年第20期11卷 4734-4739页

作者： Fleur Cordier Anne Hoorens Liesbeth Ferdinande jo van dorpe David Creytens Department of Pathology Ghent University HospitalGhent 9000Belgium

Inflammatory myofibroblastic tumor(IMT)of the biliary tract is rare,and often difficult to diagnose or to distinguish from other tumors due to its atypical clinical presentation and nonspecific radiological ***,IMTs are(myo)fibroblastic neoplasms with a prominent inflammatory *** are characterized by receptor tyrosine kinase gene rearrangements,most often involving an anaplastic lymphoma kinase(ALK)*** final diagnosis of IMT depends on histopathology and immunohistochemical *** this manuscript,we provide a clinical and morphomolecular overview of IMT and the difficulties that may arise in using immunohistochemical and molecular techniques in diagnosing IMT.

关键词： Inflammatory myofibroblastic tumor Fluorescence in situ hybridization Nextgeneration sequencing Mesenchymal tumors of the gastrointestinal tract

Atm deficient zebrafish model reveals conservation of the tumour suppressor function and a role in fertility

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Genes & Diseases 2023年第2期10卷 381-384页

作者： Jeroen Vierstraete Charlotte Fieuws David Creytens jo van dorpe Andy Willaert Anne Vral Kathleen BM Claes Center for Medical Genetics Department of Biomolecular MedicineGhent University Hospital and Ghent University9000 GhentBelgium Department of Human Structure and Repair Ghent University9000 GhentBelgium Cancer Research Institute Ghent (CRIG) Ghent University and Ghent University Hospital9000 GhentBelgium Department of Pathology Ghent University and Ghent University Hospital9000 GhentBelgium

Biallelic loss-of-function variants in ATM (Ataxia Telangiectasia Mutated) cause Ataxia Telangiectasia (AT), a rare disorder associated with cerebellar degeneration and ataxia, cancer predisposition, infertility, growth retardation, etc. ATM is a phosphoinositide 3-kinase-related kinase (PIKK) with a role in DNA repair and maintenance of genome stability. Studying a multisystem genetic disease like AT requires animal models to ascertain its pathogenesis at the level of tissues, organs and the organism. Due to its small size, cheap maintenance, large progeny, rapid development and initial transparency, zebrafish (Danio rerio) is an increasingly popular vertebrate model organism, suitable for genetic modifications and large-scale in vivo therapeutic screens as embryos are chemically permeable to small compounds. Currently, no zebrafish model for AT exists.1 We generated atm knock-outs through CRIPSR-Cas9 mutagenesis. We show that atm conserved its function as a tumour suppressor gene and is involved in gametogenesis and fertility. Therefore, this mutant is of great value for further studies investigating the role of atm in reproduction and tumorigenesis.

关键词： ATM function transparency

Pediatric vascular tumors of the liver:Review from the pathologist’s point of view

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World journal of Hepatology 2021年第10期13卷 1316-1327页

作者： Fleur Cordier Anne Hoorens jo van dorpe David Creytens Department of Pathology Ghent University HospitalGhent UniversityGhent 9000Belgium

Differential diagnosis of pediatric vascular liver tumors can be challenging due to inconsistent nomenclature,histologic overlap and the rarity of some *** we give an up-to-date overview of the most important *** discuss the clinic,histology and pathophysiology of hepatic congenital and infantile heman-gioma,hepatic epithelioid hemangioendothelioma and hepatic angio-sarcoma.

关键词： Hepatic congenital hemangioma Hepatic infantile hemangioma Hepatic epithelioid hemangioendothelioma Hepatic angiosarcoma Hepatic vascular tumors of infancy Hepatic hemangiomas

Immune response to inactivated bacterial vector carrying the recombinant K39 antigen of Leishmania infantum in mice

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Asian Pacific journal of Tropical Biomedicine 2024年第5期14卷 199-206页

作者： Lucelina S.Araújo Bruno B.Silva Eduarda N.F.N.Santos Arnaldo S.Bezerra Samuel S.Frota Assis R.Montenegro Eridan O.P.T.Florean Maurício Fvan Tilburg Maria Izabel F.Guedes Laboratory of Biotechnology and Molecular Biology Northeast Biotechnology NetworkState University of CearáFortalezaBrazil Faculty of Veterinary Medicine State University of CearáFortalezaBrazil

Objective:To evaluate the immunological response elicited by an inactivated bacterial vector carrying the K39 antigen of Leishmania infantum,and a purified ***:Mice were subjected to the following treatments:(1)Purified recombinant K39(rK39)protein at a 20μg dose with complete Freund’s adjuvant;(2)Inactivated Escherichia coli(BL21 DE3)carrying the K39 protein at an equivalent total protein content of 200μg;(3)Inactivated bacteria lacking the K39 protein;(4)Non-immunized control *** monitoring was *** groups were challenged by intraperitoneal injection of 10^(7) Leishmania infantum *** euthanasia,the liver and spleen were collected to analyze the levels of TNF,IFN-γ,IL-12,IL-4,and ***:Mice immunized with purified rK39 or the inactivated bacterial vector carrying the K39 antigen of Leishmania infantum showed a long-lasting immune response with high levels of polyclonal antibodies specifically recognizing the recombinant *** IgG1 subclass was the predominant immunoglobulin;however,the induction of IgG2a and the profile of cytokines produced were indicative of the induction of a mixed-type ***:The inactivated bacterial vector carrying the K39 antigen,as well as the purified antigen can induce a long-lasting immune response in immunized mice,predominantly favouring a Th2 profile response.

关键词： Visceral leishmaniasis K39 Inactivated bacterial vector Vaccine Immune response Th1 Th2 Leishmania infantum

Earwig mothers consume the feces of their juveniles during family life

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Insect Science 2022年第2期29卷 595-602页

作者： Sophie van Meyel Séverine Devers joël Meunier Institut de Recherche sur la Biologie de l'Insecte UMR 7261CNRSUniversity of ToursToursFrance

Many animals consume the feces of their *** allo-coprophagy can have benefits,such as access to nutrients and symbionts,but also risks for consumers,mainly due to direct contact with pathogens that develop on *** the European earwig Forficula auricularia,mothers and juveniles live in nests lined with their *** surprising habit allows juveniles to consume the feces of their siblings during family life and provides them with nutritional benefits when mothers provide low ***,it was unclear whether earwig mothers also practice allo-coprophagy,and whether this behavior is motivated by their nutritional ***,we set up four types of experimental families in which we manipulated the nutritional needs of mothers and/or juveniles and measured the effects on the production of feces by the juveniles,and the consumption of these feces by the *** results first show that fed juveniles produced more feces pellet in presence of fed compared to food-deprived *** also found that,overall,about 50%of the mothers consumed juveniles *** consumption was both more likely and larger when the feces were produced by fed compared to food-deprived juveniles,while the proportion of feces pellets eaten was larger in food-deprived compared to fed ***,our results reveal that allo-coprophagy involves every family member and suggest that it can have both nutritional and non-nutritional benefits for earwig ***-coprophagy could thus favor the maintenance of mothers in the nest and,more generally,promote the early evolution of family life.

关键词： cooperation coprophagy dermaptera family life parental care

Scalable fabrication,compartmentalization and applications of living microtissues

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Bioactive Materials 2023年第1期19卷 392-405页

作者： Maik Schot Nuno Araújo-Gomes Bas van Loo Tom Kamperman Jeroen Leijten Department of Developmental Bioengineering Faculty of Science and TechnologyTechnical Medical CentreUniversity of TwenteDrienerlolaan 57522NBEnschedethe Netherlands

Living microtissues are used in a multitude of applications as they more closely resemble native tissue physiology,as compared to 2D *** are typically composed of a combination of cells and materials in varying combinations,which are dictated by the applications’design *** applications range wide,from fundamental biological research such as differentiation studies to industrial applications such as cruelty-free meat ***,their translation to industrial and clinical settings has been hindered due to the lack of scalability of microtissue production *** microfluidic processes provide an opportunity to overcome this limitation as they offer higher throughput production rates as compared to traditional batch techniques,while maintaining reproducible control over microtissue composition and *** this review,we provide a comprehensive overview of the current approaches to engineer microtissues with a focus on the advantages of,and need for,the use of continuous processes to produce microtissues in large ***,an outlook is provided that outlines the required developments to enable large-scale microtissue fabrication using continuous processes.

关键词： Tissue engineering Micromaterials Microfluidics Upscaling Clinical translation Industrial translation

Chemoattractants and cytokines in primary ciliary dyskinesia and cystic fibrosis: key players in chronic respiratory diseases

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Cellular & Molecular Immunology 2018年第4期15卷 312-323页

作者： Maaike Cockx Mieke Gouwy jo van Damme Sofie Struyf Laboratory of Molecular Immunology Department of Microbiology and ImmunologyRega Institute for Medical ResearchUniversity of LeuvenLeuven 3000Belgium

Patients with primary ciliary dyskinesia(PCD)and cystic fibrosis(CF),two inherited disorders,suffer from recurrent airway infections characterized by persistent bacterial colonization and uncontrollable *** present in high counts,neutrophils fail to clear infection in the *** levels of C-X-C motif chemokine ligand 8/interleukin-8(CXCL8/IL-8),the most potent chemokine to attract neutrophils to sites of infection,are detected in the sputum of both patient groups and might cause the high neutrophil influx in the ***,in CF,airway neutrophils are highly activated because of the genetic defect and the high levels of proinflammatory chemoattractants and cytokines(e.g.,CXCL8/IL-8,tumor necrosis factor-αand IL-17).The overactive state of neutrophils leads to lung damage and fuels the vicious circle of infection,excessive inflammation and tissue *** inflammatory process in CF airways is well characterized,whereas the lung pathology in PCD is far less *** knowledge of CF lung pathology could be useful to guide molecular investigations of the inflammatory processes in PCD *** available therapies can not completely remedy the chronic airway infections in these *** review gives an overview of the role that chemoattractants and cytokines play in these neutrophil-dominated lung ***,the most frequently applied treatments in CF and PCD and new experimental therapies to reduce neutrophil-dominated airway inflammation are described.

关键词： chemoattractants cystic fibrosis cytokines primary ciliary dyskinesia

Reversed-phase fused-core HPLC modeling of peptides

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journal of Pharmaceutical Analysis 2013年第2期3卷 93-101页

作者： Matthias D'Hondt Bert Gevaert Sofie Stalmans Sylvia van dorpe Evelien Wynendaele Kathelijne Peremans Christian Burvenich Bart De Spiegeleer Drug Quality and Registration(DruQuaR) group Faculty of Pharmaceutical SciencesGhent UniversityHarelbekestraat 72B-9000 GhentBelgium Departments of Medical Imaging and Physiology Faculty of Veterinary MedicineGhent UniversitySalisburylaan 133B9820 MerelbekeBelgium

Different fused-core stationary phase chemistries（C18,Amide,Phenyl-hexyl and Peptide ES-C18） were used for the analysis of 21 structurally representative model *** addition,the effects of the mobile phase composition（ACN or MeOH as organic modifier;formic acid or acetic acid,as acidifying component） on the column selectivity,peak shape and overall chromatographic performance were *** RP-amide column,combined with a formic acid-acetonitrile based gradient system,performed as best.A peptide reversed-phase retention model is proposed,consisting of 5 variables：log SumAA,log Sv,clog P,log nHDon and log *** structure-retention relationship（QSRR） models were constructed for 16 different chromatographic *** accuracy of this peptide retention model was demonstrated by the comparison between predicted and experimentally obtained retention times,explaining on average 86% of the ***,using an external set of 5 validation peptides,the predictive power of the model was also *** peptide retention model includes the novel in-silico calculated amino acid descriptor,AA,which was calculated from log P,3D-MoRSE,RDF and WHIM descriptors.

关键词： Peptides Fused-core(core-shell core-enhanced poro-shell HALO s) stationary phases RP-HPLC peptide retention model In-silico amino acid descriptor

Silicon and silicon nitride photonic circuits for spectroscopic sensing on-a-chip [Invited]

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Photonics Research 2015年第5期3卷 47-59页

作者： Ananth Z.Subramanian Eva Ryckeboer Ashim Dhakal Frédéric Peyskens Aditya Malik Bart Kuyken Haolan Zhao Shibnath Pathak Alfonso Ruocco Andreas De Groote Pieter Wuytens Daan Martens Francois Leo Weiqiang Xie Utsav Deepak Dave Muhammad Muneeb Pol van dorpe joris van Campenhout Wim Bogaerts Peter Bienstman Nicolas Le Thomas Dries van Thourhout Zeger Hens Gunther Roelkens Roel Baets Photonics Research Group INTEC Department Ghent University-imec Centre for Nano and Biophotonics Ghent University University of California Department of Molecular Biotechnology Ghent University imec Kapeldreef 75 Physics and Chemistry of Nanostructures Ghent University

There is a rapidly growing demand to use silicon and silicon nitride（Si3N4） integrated photonics for sensing applications, ranging from refractive index to spectroscopic sensing. By making use of advanced CMOS technology,complex miniaturized circuits can be easily realized on a large scale and at a low cost covering visible to mid-IR wavelengths. In this paper we present our recent work on the development of silicon and Si3N4-based photonic integrated circuits for various spectroscopic sensing applications. We report our findings on waveguide-based absorption, and Raman and surface enhanced Raman spectroscopy. Finally we report on-chip spectrometers and on-chip broadband light sources covering very near-IR to mid-IR wavelengths to realize fully integrated spectroscopic systems on a chip.

关键词： Invited SOI mode

Vaccination of broiler chickens with dry powder vaccines as an alternative for liquid spray and aerosol vaccination

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Vaccination of broiler chickens with dry powder vaccines as ...