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转基因耐除草剂玉米VCO-?/1981-5定性PCR标准检测方法的建立

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热带作物学报 2017年第12期38卷 2318-2325页

作者：李美英夏启玉肖苏生贺萍萍杨小亮郭静远郭安平中国热带农业科学院热带生物技术研究所农业部热带作物生物技术与遗传资源利用重点实验室农业部转基因植物及植物用微生物环境安全监督检验测试中心

转基因耐除草剂玉米VCO-?/1981-5是由加拿大Genective SA公司通过农杆菌侵染幼胚转化获得的1种新的抗草甘膦除草剂转基因玉米转化体,目前已经进行商业化生产应用。本研究的目的是建立转基因VCO-?/1981-5玉米品系特异性转化事件标准化定... 详细信息

转基因耐除草剂玉米VCO-?/1981-5是由加拿大Genective SA公司通过农杆菌侵染幼胚转化获得的1种新的抗草甘膦除草剂转基因玉米转化体,目前已经进行商业化生产应用。本研究的目的是建立转基因VCO-?/1981-5玉米品系特异性转化事件标准化定性PCR(polymerase chain reaction,PCR)检测方法。根据VCO-?/1981-5玉米插入的外源基因和左、右侧翼序列设计了一系列特异性的PCR引物,分别进行了引物筛选和PCR反应体系优化,建立了耐除草剂玉米VCO-?/1981-5转化体特异性定性PCR标准化检测方法,对建立的检测方法在非转基因VCO-?/1981-5玉米的混合样品中进行了特异性测试,仅从含有VCO-?/1981-5玉米样品中扩增到目的条带,表明检测方法具有很好的特异性,检测的灵敏度为0.1%。建立的检测方法经过实验室内和实验间循环验证比对,具有良好的重复性和再现性。本研究建立的VCO-?/1981-5玉米定性PCR标准化方法适用于产品中含有VCO-?/1981-5玉米转基因成分的检测。

关键词： VCO-?1981-5 定性PCR 灵敏度品系特异性标准化检测方法

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用酶处理红细胞的解离试验检测毛发血型

法医学杂志 1986年第4期 7-10页

作者：蔡锐波黄长彬柳燕王明陈丽娴郭景元中山医科大学法医物证学教研室

自1966年矢田昭一等用热解离法对生物硬组织进行ABO血型测定获得成功以来,这一方法已被广泛应用于毛发的血型测定.有关的研究,国内外已有不少的报道.本文对应用木瓜酶处理红细胞作解离试验判定毛发血型的准确性问题,作了一些实验研究,... 详细信息

自1966年矢田昭一等用热解离法对生物硬组织进行ABO血型测定获得成功以来,这一方法已被广泛应用于毛发的血型测定.有关的研究,国内外已有不少的报道.本文对应用木瓜酶处理红细胞作解离试验判定毛发血型的准确性问题,作了一些实验研究,现报告如下:

关键词：毛发解离试验 ABO 皮肤血型红细胞红血球酶处理

KNbO_3倍频产生488.5nm ps蓝光

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中国激光 1991年第2期18卷 114-116页

作者：邹群耿兆华赵庆春陆雨田董景元郭晶华何慧娟南京大学物理系 210008 中国科学院上海光机所激光技术开放实验室 201800

用6.9×5.3×3.3mm^3的KNbO_3晶体对光学参量放大系统输出的波长连续可调近红外光进行倍频,产生485～493nm连续可调的ps蓝光。488.5nm的能量转换效率为2.6％,峰值功率大于2kW。

关键词： KNbo3晶体倍频 PS蓝光

Effects of Polymers on Phase Transition of ε-CL-20and CL-20-Based PBXs

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Journal of Beijing Institute of Technology 2018年第3期27卷 451-458页

作者： Hang Li Xueyong guo Qingjie Jiao jingyuan Zhang Honglei Zhang Zhenghong Wang Shengquan Chang State Key Laboratory of Explosion Science and Technology Beijing Institute of Technology Liaoning Qingyang Special Chemical Co.Ltd

The powder X-ray diffraction technique is applied to investigate phase transitions ofε-CL-20 coated with additives(polymers and wax)and ofε-CL-20 heated in petroleum ester at different temperature ***,CL-20-based plastic-bonded explosives(PBXs)were prepared by mechanical *** of different polymer binders were measured and the relationship between polymer properties and sensitivity of CL-20-based PBXs was *** results indicated thatε-CL-20 coated with or without additives both showed high stability in the crystal phases at different temperature in petroleum *** with thermal conductivity,friction efficient and specific heat capacity,hardness of polymers has the most dominant effect on the sensitivity of CL-20-based *** rubber(BR)showed the best desensitizing effect among the chosen *** of EPDM and other several polymers(C-EPDM)as the polymer binder has higher press density and a lower expansion rate.

关键词： CL-20 PBX phase transition sensitivity charge density

BMP-IHH-mediated interplay between mesenchymal stem cells and osteoclasts supports calvarial bone homeostasis and repair

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Bone Research 2018年第4期6卷 355-367页

作者： Yuxing guo Yuan Yuan Ling Wu Thach-Vu Ho Junjun Jing Hideki Sugii jingyuan Li Xia Han Jifan Feng Chuanbin guo Yang Chai Center for Craniofacial Molecular Biology University of Southern California Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology In Vitro Cell Research LLC State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University Division of Endodontology Kyushu University Hospital Kyushu University

Calvarial bones are connected by fibrous sutures. These sutures provide a niche environment that includes mesenchymal stem cells(MSCs), osteoblasts, and osteoclasts, which help maintain calvarial bone homeostasis and repair. Abnormal function of osteogenic cells or diminished MSCs within the cranial suture can lead to skull defects, such as craniosynostosis. Despite the important function of each of these cell types within the cranial suture, we have limited knowledge about the role that crosstalk between them may play in regulating calvarial bone homeostasis and injury repair. Here we show that suture MSCs give rise to osteoprogenitors that show active bone morphogenetic protein(BMP) signalling and depend on BMP-mediated Indian hedgehog(IHH) signalling to balance osteogenesis and osteoclastogenesis activity. IHH signalling and receptor activator of nuclear factor kappa-Β ligand(RANKL) may function synergistically to promote the differentiation and resorption activity of osteoclasts. Loss of Bmpr1a in MSCs leads to downregulation of hedgehog(Hh) signalling and diminished cranial sutures. Significantly, activation of Hh signalling partially restores suture morphology in Bmpr1a mutant mice, suggesting the functional importance of BMP-mediated Hh signalling in regulating suture tissue homeostasis. Furthermore, there is an increased number of CD200+ cells in Bmpr1a mutant mice, which may also contribute to the inhibited osteoclast activity in the sutures of mutant mice. Finally, suture MSCs require BMPmediated Hh signalling during the repair of calvarial bone defects after injury. Collectively, our studies reveal the molecular and cellular mechanisms governing cell–cell interactions within the cranial suture that regulate calvarial bone homeostasis and repair.

关键词： BMP-IHH-mediated interplay between mesenchymal stem cells and osteoclasts supports calvarial bone homeostasis and repair RANKL

β-CD改性PVDF-HFP复合聚乙烯凝胶聚合物电解质的性能

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膜科学与技术 2025年第1期45卷 11-19页

作者：李明晔李钒周敬源郭红霞北京工业大学材料科学与工程学院北京100124 江苏北星新材料科技有限公司常州213300

采用溶液浇铸法将含β-环糊精(β-CD)添加剂的聚偏氟乙烯-六氟丙烯(PVDF-HFP)浇涂于预处理的聚乙烯(PE)基体上,制备了PVDF-HFP/β-CD@PE复合凝胶聚合物电解质.利用SEM、FTIR、交流阻抗法、计时电流法等对凝胶聚合物电解质膜的表面形貌... 详细信息

采用溶液浇铸法将含β-环糊精(β-CD)添加剂的聚偏氟乙烯-六氟丙烯(PVDF-HFP)浇涂于预处理的聚乙烯(PE)基体上,制备了PVDF-HFP/β-CD@PE复合凝胶聚合物电解质.利用SEM、FTIR、交流阻抗法、计时电流法等对凝胶聚合物电解质膜的表面形貌、结构等物理和电化学性能进行表征,探究了不同β-CD添加量对PVDF-HFP/β-CD@PE复合凝胶聚合物电解质的结构与电化学性能的影响.结果表明,当β-CD添加质量分数为5.0%时,得到的复合凝胶聚合物电解质的离子电导率、锂离子迁移数和电化学稳定窗口分别为4.85×10^(-4)S/cm、0.77和5.2 V(vs Li^(+)/Li),以其组装的Li||LiFePO4电池的放电比容量为160.4 mAh/g,循环150圈仍保持稳定.

关键词：聚烯烃隔膜聚偏氟乙烯-六氟丙烯共聚物 β-环糊精凝胶聚合物电解质

Construction of an intranasal drug delivery system with hypothalamus-targeting nanoparticles

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SusMat 2024年第4期4卷 65-76页

作者： Qianru Rao Yujie Xu Xiaorong Wang Hang Luo Haoqi Li jingyuan Xiong Huile Gao guo Cheng Laboratory of Molecular Translational Medicine Center for Translational MedicineKey Laboratory of Birth Defects and Related Diseases of Women and Children(Sichuan University)Ministry of EducationMaternal&Child Nutrition CenterWest China Second University HospitalSichuan UniversityChengduChina Key Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry West China School of PharmacySichuan UniversityChengduChina Healthy Food Evaluation Research Center West China School of Public Health and West China Fourth HospitalSichuan UniversityChengduChina

Dysfunction of the hypothalamus is associated with endocrine imbalances,growth abnormalities,and reproductive ***,there is a lack of targeted treatment strategies focused on the *** this study,we constructed a multifunctional nanocarrier system(S@ANP)to directly target the hypothalamic neurokinin receptor 3(NK3R)via an intranasal delivery *** system could overcome the primary obstacles in drug delivery for hypothalamus-related *** the guidance of a modified(Trp7,β-Ala8)-neurokinin A(4-10)peptide with cysteine,nanoparticles encapsulated with SB222200,an NK3R inhibitor,were found to readily penetrate hypothalamic cells with substantial loading capacity,encapsulation efficiency,and sustained release in ***,intranasal delivery represents an optimal delivery strategy that allows for a significant reduction in oral dosage and enables nanoparti-cles to bypass the blood-brain barrier and target relevant parts of the *** mucolytic agent N-acetyl-L-cysteine(NAC)was loaded into the nanopar-ticles(S@ANP+NAC)to increase mucosal solubility and intranasal delivery *** vivo evaluations showed that S@ANP+NAC could effectively tar-get the hypothalamus and modulate NK3R-regulated hypothalamic functions in *** to its high hypothalamic targeting efficiency and low toxicity,this intranasal nanoparticle drug delivery system may serve as a potential strategy for precision therapy of hypothalamic disorders.

关键词： hypothalamus intranasal delivery nanoparticles neurokinin receptor 3

四氢姜黄素及其纳米制剂对脂多糖诱导的抑郁小鼠的改善作用

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安徽医科大学学报 2025年第1期60卷 79-86页

作者：谭慧李远萍孟靖源马滕滕杨艳杨正茂马加庆谢建平郭英昆明医科大学基础医学院药理学系昆明650500 浦瑞生物医药技术有限公司湖州313000 云南民族大学图书馆技术服务部昆明650500

目的研究四氢姜黄素(THC)和纳米四氢姜黄素(THCN)的抗抑郁作用及机制。方法将46只雄性ICR小鼠随机分为Con组、脂多糖(LPS)组、THC组、THCN组和舍曲林(SER)组。采用腹腔注射LPS建立小鼠抑郁模型。采用旷场实验和强迫游泳实验评估小鼠的... 详细信息

目的研究四氢姜黄素(THC)和纳米四氢姜黄素(THCN)的抗抑郁作用及机制。方法将46只雄性ICR小鼠随机分为Con组、脂多糖(LPS)组、THC组、THCN组和舍曲林(SER)组。采用腹腔注射LPS建立小鼠抑郁模型。采用旷场实验和强迫游泳实验评估小鼠的焦虑及抑郁样行为;髓鞘染色法检测长期实验小鼠前额皮层脱髓鞘程度;免疫荧光法检测短期实验小鼠前额皮层和海马组织中胶质纤维酸性蛋白(GFAP)和Toll样受体4(TLR4)表达水平。结果与Con组相比,长期和短期实验LPS组小鼠焦虑抑郁样行为均增加(P<0.05);长期实验LPS组小鼠脱髓鞘程度增加(P<0.01);短期实验LPS组小鼠GFAP表达减少(P<0.01),TLR4表达增加(P<0.05),THC组小鼠TLR4表达减少(P<0.01),THCN组前额皮层的GFAP表达减少(P<0.01),TLR4表达增加(P<0.05)。与LPS组相比,长期实验THC组小鼠抑郁样行为减少(P<0.05),THCN组和SER组小鼠焦虑抑郁样行为减少(P<0.05),短期实验THC组、THCN组小鼠焦虑抑郁样行为减少(P<0.05);长期实验THC组、THCN组和SER组小鼠脱髓鞘程度均减少(P<0.05);短期实验THC组GFAP表达增加(P<0.05),TLR4表达减少(P<0.05),THCN组GFAP表达增加(P<0.05)。与THC组相比,长期实验THCN组、SER组焦虑样行为减少(P<0.05);短期实验THCN组前额皮层的GFAP表达减少(P<0.05),海马DG区TLR4表达增加(P<0.01)。结论THC和THCN均能改善脂多糖诱导的小鼠抑郁样行为和脱髓鞘化,THC的抗抑郁机制可能与下调TLR4、增加GFAP有关,THCN的抗抑郁机制可能与增加GFAP有关。

关键词：四氢姜黄素抑郁症 Toll样受体4 胶质纤维酸性蛋白髓鞘脂多糖

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An Approach to Carbon Emissions Prediction Using Generalized Regression Neural Network Improved by Genetic Algorithm

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Electrical Science & Engineering 2020年第1期2卷 4-10页

作者： Zhida guo jingyuan Fu School of Economics and Management Dalian Jiaotong UniversityDalianChina Academic Unit of Human Communication Developmentand Information SciencesFaculty of educationthe University of Hong KongHong KongChina

The study on scientific analysis and prediction of China’s future carbon emissions is conducive to balancing the relationship between economic development and carbon emissions in the new era,and actively responding to climate change *** the analysis of the application of the generalized regression neural network(GRNN)in prediction,this paper improved the prediction method of *** algorithm(GA)was adopted to search the optimal smooth factor as the only factor of GRNN,which was then used for prediction in *** the prediction of carbon dioxide emissions using the improved method,the increments of data were taken into *** target values were obtained after the calculation of the predicted ***,compared with the results of GRNN,the improved method realized higher prediction *** thus offers a new way of predicting total carbon dioxide emissions,and the prediction results can provide macroscopic guidance and decision-making reference for China’s environmental protection and trading of carbon emissions.

关键词： Carbon emissions Genetic Algorithm Generalized Regression Neural Network Smooth Factor Prediction