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In vivo observation of cerebral microcirculation after experimental subarachnoid hemorrhage in mice

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Neural Regeneration Research 2018年第3期13卷 456-462页

作者： Xiao-mei yang Xu-hao Chen Jian-fei Lu Chang-man Zhou jing-yan han Chun-hua Chen Department of Human Anatomy and Embryology Peking University Health Science Center School of Basic Medical Sciences Peking University Health Science Center Tasly Microcirculation Research Center Peking University Health Science Center

Acute brain injury caused by subarachnoid hemorrhage is the major cause of poor prognosis. The pathology of subarachnoid hemorrhage likely involves major morphological changes in the microcirculation. However, previous studies primarily used fixed tissue or delayed injury models. Therefore, in the present study, we used in vivo imaging to observe the dynamic changes in cerebral microcirculation after subarachnoid hemorrhage. Subarachnoid hemorrhage was induced by perforation of the bifurcation of the middle cerebral and anterior cerebral arteries in male C57/BL6 mice. The diameter of pial arterioles and venules was measured by in vivo fluorescence microscopy at different time points within 180 minutes after subarachnoid hemorrhage. Cerebral blood flow was examined and leukocyte adhesion/albumin extravasation was determined at different time points before and after subarachnoid hemorrhage. Cerebral pial microcirculation was abnormal and cerebral blood flow was reduced after subarachnoid hemorrhage. Acute vasoconstriction occurred predominantly in the arterioles instead of the venules. A progressive increase in the number of adherent leukocytes in venules and substantial albumin extravasation were observed between 10 and 180 minutes after subarachnoid hemorrhage. These results show that major changes in microcirculation occur in the early stage of subarachnoid hemorrhage. Our findings may promote the development of novel therapeutic strategies for the early treatment of subarachnoid hemorrhage.

关键词： nerve regeneration subarachnoid hemorrhage microcirculation disturbance leukocyte adhesion albumin extravasation cerebral blood flow vasoconstriction early brain injury neural regeneration

Clinical study on pediatric Fisher's syndrome in Twenty-two cases

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白求恩医科大学学报 2001年第2期27卷 181-183页

作者： SUN jing-hui,han yan-yan,LIU Li,LU Ji-rong (Department of Pediatrics, First Hospital,Jilin University, Changchun 130021 China)[ 吉林大学附属第一医院吉林长春 130021

目的 :进一步明确小儿 Fisher氏综合征的病因及诊断标准。方法 :对 2 2例小儿 Fisher氏综合征的临床表现、脑脊液进行对比研究。结果 :2 2例患儿均以眼肌麻痹和小脑共济失调为主征 ,伴有肢体不同程度的不完全的弛缓性瘫痪 ,约半数以上... 详细信息

目的 :进一步明确小儿 Fisher氏综合征的病因及诊断标准。方法 :对 2 2例小儿 Fisher氏综合征的临床表现、脑脊液进行对比研究。结果 :2 2例患儿均以眼肌麻痹和小脑共济失调为主征 ,伴有肢体不同程度的不完全的弛缓性瘫痪 ,约半数以上病儿脑脊液出现蛋白、细胞分离现象。结论 :Fisher氏综合征是急性感染性神经根神经炎 ( AIP)一种特殊型或变异型 ,是以小脑共济失调为主的颅神经受损的AIP。

关键词：儿童 Fisher氏综合征病因诊断标准临床表现脑脊液

Total salvianolic acid improves ischemia-reperfusion-induced microcirculatory disturbance in rat mesentery

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World Journal of Gastroenterology 2010年第42期16卷 5306-5316页

作者： Ming-Xia Wang Yu-Ying Liu Bai-He Hu Xiao-Hong Wei Xin Chang Kai Sun jing-Yu Fan Fu-Long Liao ChuanShe Wang Jun Zheng jing-yan han Ming-Xia Wang Tasly Microcirculation Research Center Peking University Health Science Center Beijing 100191 China Department of Pharmacology Tianjin University of Traditional Chinese Medicine Tianjin 300193 China Tasly Microcirculation Research Center Peking University Health Science Center Beijing 100191 China Department of Integration of Chinese and Western Medicine School of Basic Medical SciencesPekingUniversityBeijing 100191China

AIM:To investigate the effect of total salvianolic acid(TSA) on ischemia-reperfusion(I/R)-induced rat mesenteric microcirculatory ***:Male Wistar rats were randomly distributed into 5 groups(n = 6 each):Sham group and I/R group(infused with saline),TSA group,TSA + I/R group and I/R + TSA group(infused with TSA,5 mg/kg per hour).Mesenteric I/R were conducted by a ligation of the mesenteric artery and vein(10 min) and subsequent release of the *** was continuously infused either starting from 10 min before the ischemia or 10 min after *** in mesenteric microcirculatory variables,including diameter of venule,velocity of red blood cells in venule,leukocyte adhesion,free radicals released from venule,albumin leakage and mast cell degranulation,were observed through an inverted intravital ***,the expression of adhesion molecules CD11b/CD18 on neutrophils was evaluated by flow *** evidence of mesenteric venules damage was assessed after microcirculation ***:I/R led to multiple responses in mesenteric post-capillary venules,including a significant increase in the adhesion of leukocytes,production of oxygen radicals in the venular wall,albumin efflux and enhanced mast cell degranulation in *** the I/R-induced manifestations were significantly reduced by pre-or post-treatment with TSA,with the exception that the I/R-induced increase in mast cell degranulation was inhibited only by pre-treatment with ***,preor post-treatment with TSA significantly attenuated the expression of CD11b/CD18 on neutrophils,reducing the increase in the number of caveolae in the endothelial cells of mesentery post-capillary venules induced by I/***:The results demonstrated that TSA protects from and ameliorates the microcirculation disturbance induced by I/R,which was associated with TSA inhibiting the production of oxygen-free radicals in the venular wall and the expression of CD1

关键词： Salvia miltiorrhiza Leukocyte adherence Oxygen-free radicals Albumin leakage Ischemiareperfusion

Panax Notoginseng Saponins Restrains Ischemia-reperfusion-induced Rat Mesenteric Microcirculatory Disturbance

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World Journal of Traditional Chinese Medicine 2015年第3期1卷 1-8页

作者： Yu Zhang Yu-Ying Liu Quan Li Chun-Shui Pan jing-Yu Fan Chuan-She Wang Kai Sun jing-yan han Department of Integration of Chinese and Western Medicine School of Basic Medical SciencesPeking University Key Laboratory of Microcirculation State Administration of Traditional Chinese Medicine of the People’s Republic of China Key Laboratory of Stasis and Phlegm State Administration of Traditional Chinese Medicine of the People’s Republic of China Tasly Microcirculation Research Center Peking University Health Science Center

Objective: To investigate the effect of Panax notoginseng saponins(PNS) on ischemia reperfusion(I/R) induced rat mesenteric microcirculatory ***: Male Wistar rats weighting 200–250 g were subjected to 10 min ligation of the superior mesenteric artery and vein, followed by60 min reperfusion. PNS(5 mg/kg/hr) was continuously administrated starting from 10 min before ischemia or 10 min after reperfusion until60 min after reperfusion via left jugular vein. Leukocytes adhesion, mast cell degranulation, endothelial peroxidation, and albumin leakage of rat mesenteric venules were observed. Serum myeloperoxidase(MPO) level, intercellular adhesion molecule-1(ICAM-1) expression and Src phosphorylation were ***: PNS ameliorated leukocyte adhesion and mast cell degranulation, while with no obvious effects on endothelial peroxidation and albumin leakage. In addition, PNS inhibited serum MPO increase, intestinal ICAM-1 expression and Src phosphorylation induced by I/***: PNS ameliorated I/R-induced leukocyte adhesion and mast cell degranulation, the former is related to its inhibition of Src phosphorylation and ICAM-1 expression.

关键词： ICAM-1 Src leukocytes adhesion mast cell degranulation panax notoginseng

Paeonol Improves Lipopolysaccharide-induced Microcirculatory Disturbance in Rat Mesentery

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World Journal of Traditional Chinese Medicine 2015年第1期1卷 37-44页

作者： Lei Dong Ang Li Mei-Li Duan Kai Sun Yu-Ying Liu Ming-Xia Wang Chuan-She Wang jing-Yu Fan jing-yan han Department of Critical Care Medicine Beijing Friendship Hospital Capital Medical University Tasly Microcirculation Research Center Peking University Health Science Center Department of Integration of Chinese and Western Medicine School of Basic Medical Sciences Peking University Key Laboratory of Microcirculation State Administration of Traditional Chinese Medicine of China Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine

Objective: To investigate the effect of paeonol on lipopolysaccheride(LPS)-induced rat mesenteric microcirculatory ***: Male Wistar rats were randomly distributed into 5 groups(n=6 in each): Sham group, LPS group, paeonol group, paeonol+LPS group, and LPS+paeonol group. Endotoximia model was conducted by continuous LPS infusion. Changes in mesenteric microcirculatory variables, including diameter of venule, velocity of red blood cells in venule, leukocyte adhesion, free radicals produced in venule and albumin leakage from venule, were observed through an inverted intravital microscope. Meanwhile, the expression of myeloperoxidase(MPO), CD18,intercellular adhesion molecule-1(ICAM-1), toll-like receptor 4(TLR4), nuclear factor-kappa B p65 subunit(NF-κB p65), activator protein-1(AP-1), and Jun N-terminal kinase(JNK) was assessed by Western ***: After infusion of LPS, the number of leukocytes adherent to venular wall, the intensity of dihydrorhodamine 123(DHR)fluorescence in the venular walls, and albumin leakage from venules were significantly increased, whereas the red blood cell velocity in venule was decreased. All the manifestations were significantly reduced by pre-treatment and post-treatment with paeonol. Moreover, paeonol significantly attenuated the expression of MPO, CD18, ICAM-1, TLR4, NF-κB p65, AP-1 and JNK in rat mesentery after ***: The results demonstrated that paeonol could protect from and ameliorate the microcirculation disturbance induced by LPS.

关键词： Paeonol Microcirculation Leukocyte adhesion Albumin leakage

Effect of notoginsenoside R1 on hepatic microcirculation disturbance induced by gut ischemia and reperfusion

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World Journal of Gastroenterology 2008年第1期14卷 29-37页

作者： Wei-Xing Chen Fang Wang, Yu-Ying Liu, Qing-Jiang Zeng, Kai Sun, Xin Xue, Xiang Li, Ji-Ying yang, Li-Hua An,Bai-He Hu, Jin-Hui yang, Chuan-She Wang, Zhi-Xin Li, Lian-Yi Liu, yan Li, Jun Zheng, Fu-Long Liao, Dong han,jing-Yu Fan, jing-yan han Fang Wang Yu-Ying Liu Qing-Jiang Zeng Kai Sun Xin Xue Xiang Li Ji-Ying yang Li-Hua An Bai-He Hu Jin-Hui yang Chuan-She Wang Zhi-Xin Li Lian-Yi Liu yan Li Jun Zheng Fu-Long Liao Dong han jing-Yu Fan jing-yan han Tasly Microcirculation Research Center Peking University Health Science Center Beijing 100083 China Department of Pharmacology Tianjin University of Traditional Chinese Medicine Tianjin 300193 China Department of Integrated Chinese and Western Medicine School of Basic Medical Sciences Peking University Beijing 100083 China

AIM： To assess the effect of notoginsenoside R1 on hepatic microcirculatory disturbance induced by gut ischemia/reperfusion （I/R） in mice. METHODS： The superior mesenteric artery （SMA） of C57/BL mice was ligated for 15 min to induce gut ischemia followed by 30-rain reperfusion. In another set of experiments, R1 was continuously infused （10 mg/kg per hour） from 10 min before I/R until the end of the investigation to study the influence of R1 on hepatic microcirculatory disturbance induced by gut I/R. Hepatic microcirculation was observed by inverted microscopy, and the vascular diameter, red blood cell （RBC） velocity and sinusoid perfusion were estimated. Leukocyte rolling and adhesion were observed under a laser confocal microscope. Thirty and 60 min after reperfusion, lactate dehydrogenase （LDH）, alanine aminotransferase （ALl＇） and aspartate transaminase （AST） in peripheral blood were determined. The expression of adhesion molecules CD11b/CD18 in neutrophils and tumor necrosis factor- alpha （TNF-α）, interleukin-6 （IL-6） and monocyte chemotactic protein-1 （MCP-1） in plasma were evaluated by flow Oltometry. E-selectin and intercellular adhesion molecule-1 （ICAM-1） in hepatic tissue were examined by ***： After gut I/R, the diameters of terminal portal venules and central veins, RBC velocity and the number of perfused sinusoids were decreased, while the leukocyte rolling and adhesion, the expression of E-selectin in hepatic vessels and CD18 in neutrophils, IL-6, MCP-1, LDH, ALT and AST were increased. R1 treatment attenuated these alterations except for IL-6 and MCP-1. CONCLUSION： R1 prevents I/R-induced hepatic microcirculation disturbance and hepatocyte injury, The effect of R1 is related to its inhibition of leukocyte rolling and adhesion by inhibiting the expression of E-selectin in endothelium and CD18 in neutrophils.

关键词： Ischemia/reperfusion Notoginsenoside R1 Leukoytes adhesion E-selectin Hepatic injury

Progression of the Wei-Qi-Ying-Xue Syndrome,Microcirculatory Disturbances,in Infectious Diseases and Treatment with Traditional Chinese Medicine

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World Journal of Traditional Chinese Medicine 2022年第2期8卷 169-180页

作者： jing-yan han Quan Li Chun-Shui Pan Kai Sun jing-Yu Fan Department of Integration of Chinese and Western Medicine School of Basic Medical SciencesPeking UniversityBeijingPeople’s Republic of China Academy of Integration of Chinese and Western Medicine Peking University Health Science CenterBeijingPeople’s Republic of China Tasly Microcirculation Research Center Peking University Health Science CenterBeijingPeople’s Republic of China Department of Integration of Chinese and Western Medicine Peking UniversityKey Laboratory of Stasis and PhlegmState Administration of Traditional Chinese Medicine of the People’s Republic of ChinaBeijingPeople’s Republic of China Department of Integration of Chinese and Western Medicine Peking UniversityBeijing Laboratory of Integrative MicroangiopathyBeijingPeople’s Republic of China Department of Integration of Chinese and Western Medicine Peking UniversityState Key Laboratory of Core Technology in Innovative Chinese MedicineBeijingPeople’s Republic of China

Lipopolysaccharide(LPS)-induced endotoxemia is a critical condition that initiates microcirculatory disturbance and may progress to multiple organ failure that threatens the lives of millions of people around the world each *** pathology of endotoxemia involves multiple insults mediated by a range of signaling *** management is required to relieve *** Chinese medicine(TCM)is a type of therapeutic that commonly contains numerous components and,thus,exhibits multitarget *** importantly,some TCM formulas have been proposed and used for effective treatment of endotoxemia-like *** the past 20 years,an increasing number of studies have explored the effects and mechanisms of these formulas and their major bioactive components on microcirculatory disturbance and organ injury caused by *** results obtained thus far provide support for the clinical use of TCM and shed light on the underlying mechanisms.

关键词： Chinese medicine hyperpermeability leukocyte-endothelium interactions lipopolysaccharide microvascular hemorrhage

Compound Danshen injection improves endotoxin-induced microcirculatory disturbance in rat mesentery

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World Journal of Gastroenterology 2007年第26期13卷 3581-3591页

作者： jing-yan han Yoshinori Horie Soichiro Miura, Yasutada Akiba Jun Guo Dan Li jing-Yu Fan Yu-Ying Liu Bai-He Hu Li-Hua An Xin Chang Man Xu De-An Guo Kai Sun Ji-Ying yang Shu-Ping Fang Ming-Ji Xian Masahiro Kizaki Hiroshi Nagata Toshifumi Hibi Department of Integration of Chinese and Western Medicine School of Basic Medical Sciences Peking University Beijing 100083 China Department of Internal Medicine School of Medicine Keio University Tokyo 160-8582 Tokyo Japan Second Department of Internal Medicine National Defense Medical College Saitama 359-8513 Saitama Japan Tasly Microcirculation Research Center Peking University Health Science Center Beijing 100083 China

AIM: To investigate the effect of compound Danshen injection on lipopolysaccharide (LPS)-induced rat mesenteric microcirculatory dysfunctions and the underlying possible mechanism by an inverted intravital microscope and high-speed video camera system. METHODS: LPS was continuously infused through the jugular artery of male Wistar rats at the dose of 2 mg/kg per hour. Changes in mesenteric microcirculation,such as diameters of arterioles and venules,velocity of RBCs in venules,leukocyte rolling,adhesion and emigration,free radicals released from post-capillary venules,FITC- albumin leakage and mast cell degranulation,were observed through an inverted intravital microscope assisted with CCD camera and SIT camera. Meanwhile,the expression of adhesion molecules CD11b/CD18 and the production of free radical in neutrophils,and the expression of intercellular adhesion molecule 1 (ICAM-1) in human umbilical vein endothelial cells (HUVECs) were quantified by flow cytometry (FACS) in vitro. RESULTS: The continuous infusion with LPS resulted in a number of responses in microcirculation,including a significant increase in the positive region of venulestained with Monastral blue B,rolling and adhesion of leukocytes,production of oxygen radical in venular wall,albumin efflux and enhanced mast cell degranulation in vivo,all of which,except for the leukocyte rolling,were attenuated by the treatment with compound Danshen injection. Experiments performed in vitro further revealed that the expression of CD11b/CD18 and the production of oxygen free radical in neutrophils,and the expression of ICAM-1 in HUVECs were increased by exposure to LPS,and they were attenuated by compound Danshen injection. CONCLUSION: These results suggest that compound Danshen injection is an efficient drug with multi-targeting potential for improving the microcirculatory disturbance.

关键词： Compound Danshen Mesenteric microci-rculation Oxygen-free radical Adhesion molecules Lipopolysaccharide

Invitation Letter for Contributions

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World Journal of Traditional Chinese Medicine 2024年第2期10卷 F0002-F0002页

作者： De-an Guo jing-yan han WJTCM

World Journal of Traditional Chinese Medicine(WJTCM),a peer reviewed and open access journal,is sponsored by WFCMS,and it is the official journal of Good Practice in Traditional Chinese Medicine Research Association(GP-TCM RA).WJTCM(ISSN 2311-8571,quarterly)aims to report the research progress in clinical efficacy and action mechanism of TCM.

关键词： journal TCM official

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