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B-cell lymphoma-2 phosphorylation at Ser70 site-related autophagy mediates puerarin-inhibited the apoptosis of MC3T3-E1 cells during osteoblastogenesis

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Journal of Traditional Chinese Medicine 2024年第1期44卷 27-34页

作者： li Xi liN Xiangquan CHEN Dongdong liU Hui the Third Clinical Medical College Fujian Medical University Department of Orthopedics Fuzhou Second HospitalFuzhou 350007China

OBJECTIVE:To explore the relationship between autophagy and apoptosis regulated by puerarin during ***:In this study,the effects of puerarin on the autophagic activity and apoptosis level of osteoblast precursors(MC3T3-E1 cells)was ***,the roles of puerarin on B-cell lymphoma-2(Bcl-2)phosphorylation at different sites in osteoblast precursors were *** effect of puerarin on the interaction between Bcl-2 and autophagy regulatory molecule or pro-apoptotic molecule was also investigated using Co-immunoprecipitation *** addition,the effect of puerarin on mitochondrial membrane potential of osteoblast precursors was also identified by mitochondrial membrane potential fluorescence probe ***:Our results showed that puerarin can promote the autophagic activity and apoptosis level of *** addition,puerarin promoted Bcl-2phosphorylation at Ser70 site,and the dissociation of Bcl-2-Beclin1 ***,puerarin could enhance the binding of Bcl-2-Bcl-2-Associated X(Bax)complex in MC3T3-E1 ***,puerarin increased the mitochondrial membrane potential of MC3T3-E1 ***:Therefore,puerarin promotes Beclin1into autophagy flux through Bcl-2 phosphorylation at Ser70,thereby enhancing autophagy of osteoblast precursors,which mediates its anti-apoptotic role during ***,the dissociation of Bcl-2-Beclin1 complex is conducive to the binding of Bcl-2-Bax complex,which resists the apoptosis of osteoblast precursors via the increased mitochondrial membrane potential.

关键词： puerarin osteoblasts autophagy apoptosis lymphoma B-cell

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北葶苈子中抗心肌细胞凋亡活性成分筛选

中国新药杂志 2024年第18期33卷 1933-1942页

作者：李静阳张莉杨方方卢仁睿李孟冯卫生郑晓珂河南中医药大学药学院郑州450046 河南省中药开发工程技术研究中心郑州450046

目的:从北葶苈子中提取、分离得到的27种化合物中筛选出对阿霉素诱导的心肌细胞损伤模型有改善作用的成分,并对其作用机制进行初步研究。方法:阿霉素梯度浓度给药筛选最佳给药浓度,采用5μg·mL-1阿霉素建立H9c2心肌细胞损伤模型,用北... 详细信息

目的:从北葶苈子中提取、分离得到的27种化合物中筛选出对阿霉素诱导的心肌细胞损伤模型有改善作用的成分,并对其作用机制进行初步研究。方法:阿霉素梯度浓度给药筛选最佳给药浓度,采用5μg·mL-1阿霉素建立H9c2心肌细胞损伤模型,用北葶苈子中化合物处理细胞24 h,检测细胞活力及乳酸脱氢酶(lactate dehydrogenase,LDH)、总超氧化物歧化酶(superoxide dismutase,T-SOD)、丙二醛(malondialdehyde,MDA)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)水平,流式细胞术检测活性氧(reactive oxygen species,ROS)、线粒体膜电位和细胞凋亡水平,In-Cell-Western法检测线粒体凋亡通路关键蛋白表达水平。结果:北葶苈子中的北葶新苷D(LS-18)、槲皮素-3-O-β-D-葡萄糖-(1→2)-β-D-葡萄糖苷(LS-22)和槲皮素-3-O-β-D-吡喃半乳糖苷(LS-26)可有效提高心肌细胞活力,降低LDH,MDA水平,升高T-SOD水平,改善氧化应激。进步一研究发现LS-18,LS-22和LS-26可降低细胞ROS水平,提高线粒体膜电位水平,抑制心肌细胞凋亡,抑制线粒体凋亡通路关键蛋白半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)表达水平,降低B淋巴细胞瘤-2相关X蛋白/B淋巴细胞瘤-2(Bcl-2-associated X protein/B lymphocytoma-2,Bax/Bcl-2)水平。结论:北葶苈子中化合物LS-18,LS-22和LS-26对阿霉素诱导的H9c2细胞损伤具有显著的保护作用,其机制可能与改善氧化应激稳态、抑制线粒体凋亡途径有关。

关键词：北葶苈子北葶新苷阿霉素心肌损伤线粒体凋亡通路氧化应激

Strong and tough chitin hydrogel constructed by dehydration and rehydration strategy

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Nano Research 2024年第9期17卷 8192-8199页

作者： Rui-Rui liu li-Bo Mao Shu-Hong Yu Division of Nanomaterials and Chemistry Hefei National Research Center for Physical Sciences at the MicroscaleDepartment of ChemistryInstitute of Biomimetic Materials and ChemistryAnhui Engineering Laboratory of Biomimetic MaterialsUniversity of Science and Technology of ChinaHefei 230026China Institute of Innovative Materials Department of Materials Science and EngineeringDepartment of ChemistrySouthern University of Science and TechnologyShenzhen 518055China

As a renewable,biocompatible,biodegradable soft material,chitin hydrogels have better advantages in stability,antibacterial activity,antifouling,cost,immunogenicity,and so on than most polymer ***,compared with other widely used polymer hydrogels with high strength and toughness,the practical applications of chitin-based hydrogels have been limited by their weak mechanical properties,such as cartilage repair and meniscus ***,we present the design and fabrication of chitin hydrogels with excellent mechanical strength and toughness by a dehydration and rehydration *** sequential dehydration and rehydration processes,the crystalline domains in the chitin hydrogels can be properly *** optimized crystallinity,the elastic modulus of the chitin hydrogels exceeds all previously reported values,and the fracture toughness is even comparable to some synthetic polymer hydrogels,while maintaining a high-water-content of about 80 wt.%.At the same water content,the mechanical properties of the chitin hydrogels are positively correlated with the hydrogel crystallinity,which proves that the change of mechanical properties of hydrogels is not simply dependent on weight *** hydrogels can be further strengthened by incorporating other biopolymers that are intrinsically weak,which makes the hydrogels promising for applications in fields such as cartilage repair and meniscus ***,the hydrogels enable loading and release of water-soluble and poorly water-soluble *** highly extendable strengthening and toughening strategy of chitin and chitin-based biopolymer hydrogels paves the way for their widely applications.

关键词： chitin hydrogel crystallinity rehydration chemical transformation strong and tough

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采用双区截光幕测速装置标定靶距的方法

光子学报 2024年第2期53卷 140-149页

作者：李敬孙忠辉倪晋平田会西安工业大学光电工程学院西安710021 中国人民解放军63853部队白城137001 陕西省光电测试与仪器技术重点实验室西安710021

针对短靶距测速光幕靶的靶距无法直接测量的问题,提出一种采用双区截光幕测速装置标定靶距的方法。在测得弹道上两点速度后,依据弹道方程推导了飞行弹丸在两点间任意时刻的瞬时速度计算式;依据弹丸穿过标定用测速装置和被标定光幕靶的... 详细信息

针对短靶距测速光幕靶的靶距无法直接测量的问题,提出一种采用双区截光幕测速装置标定靶距的方法。在测得弹道上两点速度后,依据弹道方程推导了飞行弹丸在两点间任意时刻的瞬时速度计算式;依据弹丸穿过标定用测速装置和被标定光幕靶的时间序列,给出了靶距标定公式。在弹丸飞行速度一定的情况下,分析标定用光幕靶的靶距和布放间距对标定误差的影响,并进行了实弹射击试验。试验结果表明,标定用光幕靶的靶距、布放间距及弹丸飞行速度在一定条件下,标定相对误差为0.8‰,所提方法能够用来标定各种原理的使用级区截测速用传感器的靶距。

关键词：速度测量光幕靶标定阻力参数误差传递

Palladium-catalyzed multi components oxy-aminofluorination and aminofluorination of gem-difluoroalkenes

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Chinese Chemical Letters 2024年第2期35卷 420-425页

作者： Fen Wu Xin li Junbiao Chang Dachang Bai State Key Laboratory of Antiviral Drugs NMPA Key Laboratory for Research and Evaluation of Innovative DrugKey Laboratory of Green Chemical Media and ReactionsMinistry of EducationSchool of Chemistry and Chemical EngineeringPingyuan LaboratoryHenan Normal UniversityXinxiang 453007China State Key Laboratory of Organometallic Chemistry Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai 200032China

Organofluorine compounds are widely used in the realm of drug discovery and material ***,we developed palladium catalyzed intermolecular aminofluorination and oxy-aminofluorination of gem‑difluoroalkenes with N-fluorobenzenesulfonimide(NFSI),in which NFSI was used as the nitrogen source and *** reaction provides an efficient and straightforward synthesis route of a series ofα-trifluoromethyl benzylic ***,three/four components oxy-aminofluorination processes were realized to giveα-trifluoromethyl benzylic ether with a terminal amino group,which proceed through C(sp^(3))-O bond cleavage of easily available ether and simultaneous introduced a fluorine,an amino and an oxy substituent in one pot with excellent *** divergent reactivity not only included the incorporation of one ether molecular,but also much more challenged two ether insertion with excellent selectivity through succession C(sp^(3))-O bonds *** protocol allows for concise synthesis of high value amines with fluoroalkyl-substituents and selectively transformation of easily available ethers by high-valent palladium catalysis.

关键词： Palladium Multi components Aminofluorination Oxy-aminofluorination C(sp^(3))-O bond cleavage

Causal Analysis Between Rice Growth and Cadmium Accumulation and Transfer under Arbuscular Mycorrhizal Inoculation

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Rice science 2024年第2期31卷 226-236页

作者： ZHAO Ting WANG li YANG Jixian MA Fang State Key Laboratory of Urban Water Resource and Environment School of EnvironmentHarbin Institute of TechnologyHarbin 150090China

Cadmium(Cd)contamination in rice has been a serious threat to human *** investigate the effects of arbuscular mycorrhizal fungi(AMF)on the Cd translocation in rice,a controlled pot experiment was *** results indicated that AMF significantly increased rice biomass,with an increase of up to 40.0%,particularly in root biomass by up to 68.4%.Notably,the number of prominent rice individuals also increased,and their plasticity was enhanced following AMF *** led to an increase in the net photosynthetic rate and antioxidant enzyme activity of *** the AMF treatment group,the Cd concentration in the rice roots was significantly higher(19.1%‒68.0%)compared with that in the control ***,the Cd concentration in the rice seeds was lower in the AMF treatment group,indicating that AMF facilitated the sequestration of Cd in rice roots and reduced Cd accumulation in the *** coefficients varied across different treatments,suggesting that AMF inoculation reduced the direct impact of soil Cd concentration on the total Cd accumulation in *** translocation of Cd was consistently associated with simultaneous growth dilution and compensatory accumulation as a result of mycorrhizal *** study quantitatively analyzed this process through path analysis and clarified the causal relationship between rice growth and Cd transfer under the influence of AMF.

关键词： cadmium transfer dilution effect heavy metal immobilization mycorrhizal effect path analysis phenotypic plasticity

Characteristics and Mechanisms of Persistent Wet–Cold Events with Different Cold-air Paths in South China

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Advances in Atmospheric Sciences 2024年第6期41卷 1171-1183页

作者： Xiaojuan SUN li CHEN Chuhan LU Panxing WANG Key Laboratory of Meteorological Disaster Ministry of Education/Collaborative Innovation Center on Forecast and Evaluation of Meteorological DisastersNanjing University of Information Science and TechnologyNanjing 210044China School of Atmospheric Sciences Nanjing University of Information Science and TechnologyNanjing 210044China Qianxinan Bouyei and Miao Autonomous Prefecture Meteorological Bureau of Guizhou Province Xingyi 562400China

We investigate the characteristics and mechanisms of persistent wet–cold events(PWCEs)with different types of coldair *** show that the cumulative single-station frequency of the PWCEs in the western part of South China is higher than that in the eastern *** pattern of single-station frequency of the PWCEs are“Yangtze River(YR)uniform”and“east–west inverse”.The YR uniform pattern is the dominant mode,so we focus on this *** cold-air paths for PWCEs of the YR uniform pattern are divided into three types—namely,the west,northwest and north types—among which the west type accounts for the largest *** differences in atmospheric circulation of the PWCEs under the three types of paths are *** thermal inversion layer in the lower troposphere is favorable for precipitation during the *** positive water vapor budget for the three types of PWCEs mainly appears at the southern boundary.

关键词： persistent wet–cold events cold-air paths circulation characteristics water vapor

Yemazhui(Herba Eupatorii lindleyani)ameliorates lipopolysaccharide-induced acute lung injury via modulation of the toll-like receptor 4/nuclear factor kappa-B/nod-like receptor family pyrin domain-containing 3 protein signaling pathway and intestinal flor

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Journal of Traditional Chinese Medicine 2024年第2期44卷 303-314页

作者： REN li HAI Yang YANG Xue LUO Xianqin Chongqing Key Laboratory of Traditional Chinese Medicine for Prevention and Cure of Metabolic Diseases College of Traditional Chinese MedicineChongqing Medical UniversityChongqing 400016China College of Basic Medicine Chongqing Medical UniversityChongqing 400016China

OBJECTIVE:To investigate the impact of Yemazhui(Herba Eupatorii lindleyani,HEL)against lipopolysaccharide(LPS)-induced acute lung injury(Ali)and explore its underlying mechanism in ***:The chemical constituents of HEL were analyzed by ultra-high performance liquid chromatographyquadrupole time-of-flight mass spectrometry ***,HEL was found to suppress LPS-induced Ali in ***-week-old male Sprague-Dawley rats were randomly divided into 6 groups:control,LPS,Dexamethasone(Dex),HEL low dose 6 g/kg(HEL-L),HEL medium dose 18 g/kg(HEL-M)and HEL high dose 54 g/kg(HEL-H)*** model rats were intratracheally injected with 3 mg/kg LPS to establish an Ali *** counts,lung wet/dry weight ratio,as well as myeloperoxidase(MPO)activity were determined followed by the detection with hematoxylin and eosin staining,enzyme linked immunosorbent assay,quantitative real time polymerase chain reaction,western blotting,immunohistochemistry,and ***,to explore the effect of HEL on Ali-mediated intestinal flora,we performed 16s rRNA sequencing analysis of intestinal ***:HEL attenuated LPS-induced inflammation in lung tissue and intestinal flora *** study indicated that HEL suppressed the lung coefficient and wet/dry weight ratio of LPS-induced Ali in rats,inhibited leukocytes exudation and MPO activity,and improved the pathological injury of lung *** addition,HEL reduced the expression of tumor necrosis factoralpha,interleukin-1beta(IL-1β)and interleukin-6(IL-6)in bronchoalveolar lavage fluid and serum,and inhibited nuclear displacement of nuclear factor kappa-B p65(NF-κBp65).And 18 g/kg HEL also reduced the expression levels of toll-like receptor 4(TLR4),myeloid differentiation factor 88,NF-κBp65,phosphorylated inhibitor kappa B alpha(phospho-IκBα),nod-like receptor family pyrin domain-containing 3 protein(NLRP3),IL-1β,and interleukin-18(IL-18)in lung tissue,and regulated intestinal flora disturban

关键词： Yemazhui(Herba Eupatorii lindleyani) acute lung injury anti-inflammation toll-like receptor 4 nuclear factor kappa-B nod-like receptor family pyrin domain-containing 3 protein signal transduction gastrointestinal microbiome

“吉林一号”宽幅01B卫星GNSS-R数据风速反演研究

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武汉大学学报（信息科学版） 2024年第1期49卷 56-67页

作者：王峰李建强杨东凯郑琦李峰辉北京航空航天大学电子信息工程学院北京100191 北京航空航天大学宇航学院北京100191 上海航天技术研究院上海201109 上海埃依斯航天科技有限公司上海201109 天津云遥宇航科技有限公司天津300000

为了初步分析和评估“吉林一号”宽幅01B卫星全球导航卫星系统反射(global navigation satellite systemreflectometry,GNSS-R)测量数据反演海面风速的有效性和可用性,建立未标定时延多普勒相关功率峰值信噪比、归一化时延波形前沿斜率... 详细信息

为了初步分析和评估“吉林一号”宽幅01B卫星全球导航卫星系统反射(global navigation satellite systemreflectometry,GNSS-R)测量数据反演海面风速的有效性和可用性,建立未标定时延多普勒相关功率峰值信噪比、归一化时延波形前沿斜率和后沿斜率反演海面风速的模型,得到了反演模型反演风速与ECMWF(European Centre for MediumRange Weather Forecasts)风速的均方根误差。当无严格数据质量控制和数据定标时,时延-多普勒图(delay-Doppler map,DDM)的峰值信噪比、时延波形前沿和后沿斜率的反演风速的均方根误差分别为3.22 m/s、3.67 m/s和3.49 m/s。当控制信噪比大于-6 dB时,DDM峰值信噪比反演的风速的均方根误差满足低风速反演精度2 m/s的需求,但数据量下降为无严格数据质量控制的78.11%;对于前沿和后沿斜率,当信噪比大于-3 dB时,均方根误差小于2 m/s,数据量下降至53.74%。结果表明“,吉林一号”宽幅01B卫星的GNSS-R数据可用于海面风速的测量。

关键词： “吉林一号”宽幅01B卫星星载GNSS-R 海面风速反演时延-多普勒图