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番茄抗坏血酸含量相关QTL定位及候选基因鉴定

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园艺学报 2024年第2期51卷 219-228页

作者：刘根忠李方曼葛平飞陶金宝张星雨叶志彪张余洋山东农业大学园艺科学与工程学院山东泰安271018 华中农业大学果蔬园艺作物种质创新与利用全国重点实验室武汉430070 湖北洪山实验室武汉430070 华中农业大学深圳营养与健康研究院武汉430070 中国农业科学院深圳农业基因组研究所岭南现代农业科学与技术广东省实验室深圳分中心广东深圳518000

以番茄果实高抗坏血酸含量材料TS-226和低抗坏血酸含量材料TS-228为试验亲本杂交和自交创制F2遗传分离群体,分别构建高抗坏血酸混池和低抗坏血酸混池,集团分离群体测序(bulked segregant analysis sequencing,BSA-seq)分析鉴定到与番茄... 详细信息

以番茄果实高抗坏血酸含量材料TS-226和低抗坏血酸含量材料TS-228为试验亲本杂交和自交创制F2遗传分离群体,分别构建高抗坏血酸混池和低抗坏血酸混池,集团分离群体测序(bulked segregant analysis sequencing,BSA-seq)分析鉴定到与番茄果实抗坏血酸相关的主效QTL,进一步将与抗坏血酸相关的候选基因定位于番茄基因组8号染色体58.00~60.15 Mb区域内。结合?SNP-index数据,推断SlPPO(Polyphenol oxidase)是参与调控番茄抗坏血酸含量的主效基因。以TS-228为背景材料,通过农杆菌介导方法进行遗传转化,获得SlPPO过表达和沉默株系,相比野生型,过表达株系OE-3和OE-18的红熟果实总抗坏血酸含量分别减少了18.89%和26.56%;沉默株系Ri-9和Ri-16红熟果实总抗坏血酸含量分别增加了37.53%和63.93%。综上所述,SlPPO为参与控制番茄红熟果实抗坏血酸含量的关键基因,对果实抗坏血酸含量起负向调控作用。

关键词：番茄集团分离群体测序抗坏血酸 QTL SlPPO

April-September minimum temperature reconstruction based on Sabina tibetica ring-width chronology in the central eastern Tibetan Plateau,China

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Journal of Forestry Research 2024年第2期35卷 134-145页

作者： Teng Li Jianfeng Peng Tsun Fung Au jinbao Li School of Geography and Remote Sensing Guangzhou UniversityGuangzhou 510006People’s Republic of China College of Geography and Environmental Science Henan UniversityKaifeng 475004People’s Republic of China Institute for Global Change Biology School for Environment and SustainabilityUniversity of MichiganAnn ArborMIUSA Department of Ecology and Evolutionary Biology University of MichiganAnn ArborMIUSA Department of Geography University of Hong KongHong Kong SARPeople’s Republic of China HKU Shenzhen Institute of Research and Innovation Shenzhen 518057People’s Republic of China

Minimum temperatures have remarkable impacts on tree growth at high-elevation sites on the Tibetan Plateau,but the shortage of long-term and high-resolution paleoclimate records inhibits understanding of recent minimum temperature *** this study,a warm season(April–September)reconstruction is presented for the past 467 years(1550–2016)based on Sabina tibetica ring-width chronology on the Lianbaoyeze Mountain of the central eastern Tibetan *** warm periods and eight cold periods were ***-term minimum temperature variations revealed a high degree of coherence with nearby *** correlations between our reconstruction and global sea surface temperatures suggest that warm season minimum temperature anomalies in the central eastern Tibetan Plateau were strongly influenced by large-scale ocean atmospheric circulations,such as the El Ni?o-Southern Oscillation and the Atlantic Multidecadal Oscillation.

关键词： Tree-ring analysis Sabina tibetica Minimum temperatures Central eastern Tibetan Plateau Climate change

A 21-bp InDel in the promoter of STP1 selected during tomato improvement accounts for soluble solid content in fruits

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Horticulture Research 2023年第3期10卷 176-188页

作者： Ying Wang Chunmei Shi Pingfei Ge Fangman Li Lihui Zhu Yaru Wang jinbao tao Xingyu Zhang Haiqiang Dong Wenxian Gai Fei Wang Zhibiao Ye Donald Grierson Wei Xu Yuyang Zhang National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops College of Horticulture and Forestry ScienceHuazhong Agricultural UniversityWuhan 430070China Plant Sciences Division School of BiosciencesUniversity of NottinghamSutton Bonington CampusLoughborough LE125RDUK Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization(Xinjiang Production and Construction Crops) College of AgricultureShihezi UniversityShihezi 832003XinjiangChina Hubei Hongshan Laboratory Wuhan 430070China

Domestication and improvement are important processes that generate the variation in genome and phonotypes underlying crop ***,during selection for certain attributes,other valuable traits may be inadvertently *** example is the decline in fruit soluble solids content(SSC)during tomato *** genetic loci for SSC have been identified,but few reports on the underlying mechanisms are *** this study we performed a genome-wide association study(GWAS)for SSC of the red-ripe fruits in a population consisting of 481 tomato accessions with large natural variations and found a new quantitative trait locus,STP1,encoding a sugar transporter *** causal variation of STP1,a 21-bp InDel located in the promoter region 1124 bp upstream of the start codon,alters its ***1 Insertion accessions with an 21-bp insertion have higher SSC than STP1Deletion accessions with the 21-bp *** of STP1 in TS-23 with high SSC using CRISPR/Cas9 greatly decreased SSC in *** vivo and in vitro assays demonstrated that ZAT10-LIKE,a zinc finger protein transcription factor(ZFP TF),can specifically bind to the promoter of STP1Insertion to enhance STP1 expression,but not to the promoter of STP1Deletion,leading to lower fruit SSC in modern *** analysis revealed that STP1 was selected during tomato *** these results together,we identified a naturally occurring causal variation underlying SSC in tomato,and a new role for ZFP TFs in regulating sugar *** findings enrich our understanding of tomato evolution and domestication,and provide a genetic basis for genome design for improving fruit taste.

关键词： CRISPR/Cas9 content sugar

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关注孩子的成长过程

上海教育科研 2008年第8期 62-63页

作者：陶金宝张丰浙江省杭州市下城区青蓝小学 310003 浙江省教育厅教研室 310003

课程改革提出要“改变课程评价过分强调甄别与选拔功能，发挥评价促进学生发展、老师提高和改进教学实践的作用。”不少老师在思想上认同这一主张，但实际教学行为却有意无意地违背了这一要求。他们或诉及自己的苦衷，诉及社会学校的功... 详细信息

课程改革提出要“改变课程评价过分强调甄别与选拔功能，发挥评价促进学生发展、老师提高和改进教学实践的作用。”不少老师在思想上认同这一主张，但实际教学行为却有意无意地违背了这一要求。他们或诉及自己的苦衷，诉及社会学校的功利要求，诉及过程性评价的繁琐与浪费。

关键词：成长过程孩子课程评价过程性评价选拔功能课程改革学生发展教学实践

Multiple-model GWAS identifies optimal allelic combinations of quantitative trait loci for malic acid in tomato

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Horticulture Research 2023年第4期10卷 23-37页

作者： Wenxian Gai Fan Yang Liangdan Yuan Saeed ul Haq Yaru Wang Ying Wang Lele Shang Fangman Li Pingfei Ge Haiqiang Dong jinbao tao Fei Wang Xingyu Zhang Yuyang Zhang National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops Huazhong Agricultural UniversityWuhan 430070China Hubei Hongshan Laboratory Wuhan 430070China College of Horticulture Northwest A&F UniversityYangling 712100China Department of Horticulture The University of Agriculture PeshawarPeshawar 25130Pakistan

Malic acid(MA)is an important flavor acid in fruits and acts as a mediator in a series of metabolic *** is important to understand the factors affecting MA metabolism for fruit flavor improvement and to understand MA-mediated biological ***,themetabolic accumulation of MA is controlled by complex heredity and environmental factors,making it difficult to predict and regulate the metabolism of *** this study,we carried out a genome-wide association study(GWAS)on MA using eight milestone models with two-environment repeats.A series of associated SNP variations were identified from the GWAS,and 15 high-confidence annotated geneswere further predicted based on linkage disequilibrium and lead *** transcriptome data of candidate geneswere explored within different tomato organs as well as various fruit tissues,and suggested specific expression patterns in fruit *** on the genetic parameters of population differentiation and SNP distribution,tomato MA content has been more influenced by domestication sweeps and less affected by improvement sweeps in the long-term history of tomato *** addition,genotype×environment interaction might contribute to the difference in domestication phenotypic data under different *** study provides new genetic insights into how tomato changed its MA content during breeding and makes available function-based markers for breeding by marker-assisted selection.

关键词： breeding GWAS metabolism

Effect of shading on ascorbic acid accumulation and biosynthetic gene expression during tomato fruit development and ripening

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Vegetable Research 2022年第1期2卷 1-8页

作者： Safir Ahmad Tamim Fangman Li Ying Wang Lele Shang Xingyu Zhang jinbao tao Yaru Wang Wenxian Gai Haiqiang Dong John Kojo Ahiakpa Muhammad Ali Mumtaz Yuyang Zhang Key Laboratory of Horticultural Plant Biology Ministry of EducationHuazhong Agricultural UniversityWuhan 430070China Hubei Hongshan Laboratory Wuhan 430070China

The purpose of the current study was to determine the effect of leaf shading,fruit shading,and a combination of both,on the accumulation of ascorbic acid(AsA)and the expression levels of AsA biosynthetic genes at the immature green,mature green,breaker,and red ripe stages of Ailsa craig tomato during fruit ***(72%reducing of light intensity)imposed on the leaves significantly reduced AsA content and AsA biosynthetic gene expression in the *** shading,fruit shading,and a combination of both significantly decreased the amount of total AsA and reduced AsA to a range of 18.5%−31.5%at mature green,breaker,and red ripe stages of tomato fruits,with no significant change at the immature green stage of ***,reducing the light intensity in tomato leaves,fruits or both resulted in reduced expression of most AsA biosynthetic genes in the fruits,except for PMM,cAPX,tAPX,and APX7 genes under leaf shading,GPI,PMI,PMM,GP1,GP2,cAPX,and tAPX genes under fruit shading,and PMM,cAPX,APX1,and APX7 genes under both *** expression level of GMP,GP1,and GalDH showed an upregulation at the red ripe stage in fruits with leaf shading,and also an up-regulation at the immature green and red ripe stages with both ***,positive correlations between expression of AsA biosynthetic genes and AsA accumulation were recorded under leaf shading,fruit shading,and both types of shading,while a negative correlation was recorded under normal conditions without shading.

关键词： ascorbic development record

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番茄YABBY基因家族的生物信息学分析

东北农业大学学报 2017年第10期48卷 11-19页

作者：陈秀玲刘思源孙婷刘智琛陶金宝赵迎新王傲雪东北农业大学园艺园林学院哈尔滨150030

YABBY基因家族是植物特有转录因子,与植物形态有关,在植物叶和花器官发育过程中起重要调控作用。试验在番茄基因组范围内鉴定出9个YABBY基因家族成员,分别位于第1、5、6、7、8、11和12号染色体。利用MEGA程序对番茄、拟南芥、大白菜和玉... 详细信息

YABBY基因家族是植物特有转录因子,与植物形态有关,在植物叶和花器官发育过程中起重要调控作用。试验在番茄基因组范围内鉴定出9个YABBY基因家族成员,分别位于第1、5、6、7、8、11和12号染色体。利用MEGA程序对番茄、拟南芥、大白菜和玉米YABBY基因家族作进化分析,进化树可分为四个亚组;利用GSDS程序分析基因结构保守性,显示Sl YABBY基因内含子保守5~6个;MEME程序作基序分析,显示Sl YABBY基序保守,经鉴定所有Sl YABBYs均具有C2C2锌指结构域及YABBY结构域。运用plant CARE软件分析顺势作用元件发现,在番茄YABBY基因上游1 000 bp序列中存在多个应答不同生物和非生物胁迫的顺式作用元件,且种类和数目不同,表明其作用于番茄生长发育和逆境胁迫过程。基因功能预测和蛋白-蛋白网络分析表明,番茄YABBY家族对根、茎、叶、花、蜜腺、心皮、胚珠等部位发育具有重要调控作用。番茄YABBY基因家族表达分析发现Sl YABBY1、Sl YABBY3和Sl YABBY4在根、茎、花、叶及果实中均无表达,Sl YABBY2和Sl YABBY9表达具有较强组织特异性,为番茄YABBY基因功能研究提供参考。

关键词： YABBY基因家族番茄生物信息学进化分析基因功能

基于PIE-Engine Studio的西藏典型湖泊总悬浮物反演

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人民长江 2024年第9期55卷 85-92,99页

作者：赵爽柳锦宝耿蔚陶星宇汪菀蔚成都信息工程大学资源环境学院四川成都610225 四川省气象灾害防御技术中心四川成都610072 四川省宜宾市屏山县气象局四川宜宾645350

总悬浮物浓度是水环境评价的重要参数之一,并且能在各个方面影响水体其他参数。青藏高原湖泊环境正处于大幅变化的时期,而其分布与动态变化与湖泊的环境息息相关。基于西藏典型湖泊29个采样点水质实测数据和LandSat 8 Collection 2遥感... 详细信息

总悬浮物浓度是水环境评价的重要参数之一,并且能在各个方面影响水体其他参数。青藏高原湖泊环境正处于大幅变化的时期,而其分布与动态变化与湖泊的环境息息相关。基于西藏典型湖泊29个采样点水质实测数据和LandSat 8 Collection 2遥感数据,使用PIE-Engine Studio平台,对比分析了基于单波段和多波段组合的多种总悬浮物浓度反演模型,选出了最优的总悬浮物浓度反演模型,并运用该模型分析了色林错、扎日南木错以及塔若错总悬浮物2013~2023年月度时空变化特征。分析结果表明:①总悬浮物对绿光、红光波段最为敏感,以波段反射率组合(B3+B4)、(B2/B3)(B2为蓝光波段,B3为绿光波段,B4为红光波段)为自变量,总悬浮物浓度为因变量的三波段模型为西藏典型湖泊总悬浮物浓度遥感反演最佳模型;②总悬浮物浓度年内变化总体存在季节规律,夏季浓度高,而春秋季低。在色林错,浓度在北部较高,高值夏季主要分布在西北岸,而秋冬季则在东北岸,受风向影响较大;在扎日南木错的西部较高;在塔若错,夏季在南部较高。3个湖泊均在11月时出现东部浓度重新升高的现象。③年降水量与各湖泊的悬浮物浓度相关性较高。年际变化中,2013~2023年,悬浮物浓度的年均值先是逐年升高,在2018年后随着年降水量的减少而逐渐下降或稳定。另外,色林错、扎日南木错、塔若错湖面面积分别增长了2.87%(约68.14 km^(2))、3.01%(约30.31 km^(2))、1.01%(约4.87 km^(2)),色林错持续扩张,扎日南木错和塔若错先快速扩张,而后萎缩,湖面面积与悬浮物浓度均值表现较同步,均是受降水量和径流的影响。研究结果可为高原湖泊水质评价提供理论依据,为水土流失、水资源保护提供科学参考。

关键词：总悬浮物 PIE-Engine Studio 遥感反演云计算高原湖泊西藏

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一种H13钢端部法兰杯形件成形工艺

锻压技术 2024年第5期49卷 17-23页

作者：张超孙红星王涛汪金保刘光辉刘华郑州机械研究所有限公司河南郑州450001 西安交通大学机械工程学院陕西西安710049 三一重机有限公司江苏昆山215300

针对4Cr5MoSiV1(ASTM H13)钢在锻造过程中易产生表面裂纹等问题,以一种H13钢的端部法兰杯形锻件为研究对象,进行成形工艺开发。首先,通过高温压缩试验获得了H13钢的真实应力-真实应变曲线。其次,结合工艺试验结果,采用有限元模拟软件DEF... 详细信息

针对4Cr5MoSiV1(ASTM H13)钢在锻造过程中易产生表面裂纹等问题,以一种H13钢的端部法兰杯形锻件为研究对象,进行成形工艺开发。首先,通过高温压缩试验获得了H13钢的真实应力-真实应变曲线。其次,结合工艺试验结果,采用有限元模拟软件DEFORM-3D分析了锻造过程中金属的流动规律和应力分布,探明了试验件裂纹产生的原因,并提出了控制坯料温度均匀性、改进锻件形状等措施。最后,通过工艺改进实现了对某型H13钢杯形件的试制。试验结果表明:H13钢小锻件在锻造过程中极易出现裂纹,要尽量保证坯料温度的均匀性,控制锻造变形的一致性和接触摩擦条件。该研究对小型H13钢端部法兰杯形件的开发和生产具有一定的指导意义。

关键词： H13钢端部法兰杯形件加热方式锻造裂纹接触摩擦条件