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Efficacy of noninvasive evaluations in monitoring inflammatory bowel disease activity: a prospective study in China

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World Journal of Gastroenterology 2017年第46期23卷 8235-8247页

作者： jin-min chen Tao Liu Shan Gao Xu-Dong Tong Fei-Hong Deng Biao Nie Department of Gastroenterology Xiangyang Central HospitalHubei University of Arts and Science Department of Gastroenterology the Sixth Affiliated Hospital of Sun Yat-sen University Guangdong Provincial Key Laboratory of Gastroenterology Department of GastroenterologyNanfang HospitalSouthern Medical University Department of Gastroenterology the First Affiliated Hospital of Jinan UniversityJinan University

AIM To optimize the efficacy of noninvasive evaluations in monitoring the endoscopic activity of inflammatory bowel disease(IBD).METHODS Fecal calprotectin(FC), clinical activity index(CDAI or CAI), C-reactive protein(CRP), erythrocyte sedimentation rate(ESR), and procalcitonin(PCT) were measured for 136 IBD patients. Also, FC was measured in 25 irritable bowel syndrome(IBS) patients that served as controls. Then, endoscopic activity was determined by other two endoscopists for colonic or ileo-colonic Crohn's disease(CICD) with the "simple endoscopic score for Crohn's disease"(SES-CD), CDrelated surgery patients with the Rutgeerts score, and ulcerative colitis(UC) with the Mayo score. The efficacies of these evaluations to predict the endoscopic disease activity were assessed by Mann-Whitney test, χ~2 test, Spearman's correlation, and multiple linear regression *** The median FC levels in CD, UC, and IBS patients were 449.6(IQR, 137.9-1344.8), 497.9(IQR, 131.7-118.0), and 9.9(IQR, 0-49.7) μg/g, respectively(P < 0.001). For FC, CDAI or CAI, CRP, and ESR differed significantly between endoscopic active and remission in CICD and UC patients, but not in CD-related surgery patients. The SES-CD correlated closely with levels of FC(r = 0.802), followed by CDAI(r = 0.734), CRP(r = 0.658), and ESR(r = 0.557). The Mayo score also correlated significantly with FC(r = 0.837), CAI(r = 0.776), ESR(r = 0.644), and CRP(r = 0.634). For FC, a cut-off value of 250 μg/g indicated endoscopic active inflammation with accuracies of 87.5%, 60%, and 91.1%, respectively, for CICD, CD-related surgery, and UC patients. Moreover, clinical FC activity(CFA) calculated as 0.8 × FC + 4.6 × CDAI showed higher area under the curve(AUC) of 0.962 for CICD and CFA calculated as 0.2 × FC + 50 × CAI showed higher AUC(0.980) for UC patients than the FC. Also, the diagnostic accuracy of FC in identifying patients with mucosal inflammation in clinical remission was reflected by an AUC of 0.91 fo

关键词： Inflammatory bowel disease Crohn’s disease Ulcerative colitis Fecal calprotectin Disease activity

How little is known about “the little brown frogs”:description of three new species of the genus Leptobrachella(Anura:Megophryidae)from Yunnan Province, China

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Zoological Research 2020年第3期41卷 292-313页

作者： jin-min chen Kai Xu Nikolay A.Poyarkov Kai Wang Zhi-Yong Yuan Mian Hou Chatmongkon Suwannapoom Jian Wang jing Che State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650223China Southeast Asia Biodiversity Research Institute Chinese Academy of SciencesYezinNay Pyi Taw 05282Myanmar Kunming College of Life Science University of the Chinese Academy of SciencesKunmingYunnan 650204China Department of Vertebrate Zoology Biological FacultyLomonosov Moscow State UniversityMoscow 119991Russia Joint Russian-Vietnamese Tropical Research and Technological Center Nghia DoCau GiayHanoiVietnam Sam Noble Oklahoma Museum of Natural History&Department of Biology University of oklahomaNormanOklahoma 73072USA Key Laboratory for Conserving Wildlife with Small Populations in Yunnan Southwest Forestry UniversityKunmingYunnan 650224China College of Continuing(Online)Education Sichuan Normal UniversityChengduSichuan 610068China School of Agriculture and Natural Resources University of PhayaoPhayao 56000Thailand College of Life Science and Technology Honghe UniversityMengziYunnan 661199China

Asian leaf-litter toads of the genus Leptobrachella represent a great anuran diversification in *** studies have suggested that the diversity of this genus is still underestimated. During herpetological surveys from 2013 to 2018, a series of Leptobrachella specimens were collected from the international border areas in the southern and western parts of Yunnan Province, *** analyses based on morphological and molecular data revealed three distinct and previously unknown lineages, which we formally describe as three new species herein. Among them, we describe a new species that occurs at the highest known elevation for Leptobrachella in China. Four species of Leptobrachella, including two new species, are found in the same reserve. Furthermore, our results suggest that the population from Longchuan County,Yunnan, may represent an additional new species of Leptobrachella, although we tentatively assigned it to Leptobrachella cf. yingjiangensis due to the small sample size examined. Lastly, we provide the first description of females of L. yingjiangensis. Our results further highlight that both micro-endemism and sympatric distributions of species are common patterns in Leptobrachella, that contribute to taxonomic and conservation challenges in these frogs. We provide an identification key for Leptobrachella known to occur in Yunnan. Given the lack of knowledge on species diversity of Leptobrachella along international border areas, we recommend that future studies include transboundary collaborative surveys.

关键词： Biodiversity hotspot Conservation Cryptic diversity Micro-endemism National border Speciation Taxonomy

A new species of Mountain Dragon(Reptilia:Agamidae:Diploderma) from the D. dymondi complex in southern Sichuan Province, China

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Zoological Research 2019年第5期40卷 456-465页

作者： Kai Wang Jia-Wei Wu Ke Jiang jin-min chen Ben-Fu Miao Cameron D. Siler jing Che State Key Laboratory of Genetic Resources and Evolution Kunming Institute of Zoology Chinese Academy of Sciences Kunming Yunnan 650223 China Sam Noble Oklahoma Museum of Natural History and Department of Biology University of Oklahoma Norman Oklahoma 73072 USA chengdu Museum of Nature Exploration Chengdu Sichuan 610017 China

Despite continuous studies on the cryptic diversity of the Diploderma flaviceps complex in Southwest China for the pastdecade, little attention has been given to other widespreadcongeners in China. Combining both morphological and phylogenetic data, we describe a new species of Diplodermafrom populations identified previously as D. dymondi in thelower Yalong River Basin in southern Sichuan Province. Thenew species is morphologically most similar to D. dymondiand D. varcoae, but it can be differentiated by a considerablegenetic divergence and a suite of morphological characters,including having taller nuchal crest scales, smaller tympana,and a distinct oral coloration. Additionally, we discuss otherputative species complexes within the genus Diploderma inChina.

关键词： A new species Mountain Dragon southern Sichuan Province

Inhibition of Proliferation Induced by Cyclin D1 Gene Silence in Human Renal Carcinoma ACHN Cells

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Chinese Journal of Cancer Research 2010年第4期22卷 316-322页

作者： Jiang Wang Feng-jin Guo An-min chen Cai-hong Yang Department of Orthopedics Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

Objective： To confirm feasibility of Cyclin D1 gene as a new target for cancer gene therapy and to verify the effectiveness of shRNA expression vector-mediated gene silencing. Methods： A RNA interference DNA template targeting Cyclin D1 gene was designed and synthesized. By ligation, the fragment was inserted into Pgenesil-1-U6 to constract the recombinant plasmid Pgenesil-shRNA-Cyclin D1. The identified recombinant plasmid was transfected into ACHN cells with lipofactamine. Cyclin D1 mRNA and protein expression was analyzed by RT-PCR and western-blotting. MTT method was used for observing cell proliferation and drawing growth curve. The cell cycle and ratios of apoptotic cell were assessed by flow cytometric detection. The ability of invasion of cell migration was detected by Transwell chamber invasive models. Results： The plasmid was constructed successfully. After interference, The expression rate of Cyclin D1 mRNA decreased to 0.10±0.04 in Cyclin D1-shRNA（experimental） group and were significantly lower than Pgenesil-NC （negative） group （0.92±0.03） and ACHN （blank control） group（0.94±0.04）（P0.05）. As well, the expression rate of Cyclin D1 protein was decreased evidently in experimental group. The results of flow cytometric detection showed that, including early and late apoptotic cells, the apoptotic ratio of experimental group increased to （37.26±0.60）% significantly, while, the negative group and blank control group were only （4.62±0.40）% and （5.95±1.20）%, respectively. The cell growth curves indicated that the proliferation of experimental group cells was inhibited significantly（P0.05） and Transwell results suggested that the abilities of invasion cells transfected with Pgenesil–CyclinD1-shRNA decreased conspicuously（P0.05）. Conclusion： The shRNA can inhibit Cyclin D1 expression, specifically and persistently. The down-regulation of Cyclin D1 expression can inhibit the proliferation and induce the apoptosis of renal cell adenocarcinoma cell line ACHN.

关键词： Renal ACHN cells RNA interference Cyclin D1 Proliferation

A new snake species of the genus Gonyosoma Wagler,1828(Serpentes:Colubridae)from Hainan Island,China

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Zoological Research 2021年第4期42卷 487-491页

作者： Li-Fang Peng Yong Zhang Song Huang Frank T.Burbrink jin-min chen Mian Hou Yi-Wu Zhu Hang Yang Ji-Chao Wang Anhui Province Key Laboratory of the Conservation and Exploitation of Biological Resource College of Life SciencesAnhui Normal UniversityWuhuAnhui 241000China Huangshan Noah Biodiversity Institute HuangshanAnhui 245000China Department of Herpetology American Museum of Natural HistoryNew York NY 10024USA College of Continuing Education Sichuan Normal UniversityChengduSichuan 610068China ministry of Education Key Laboratory for Ecology of Tropical Islands College of Life SciencesHainan Normal UniversityHaikouHainan 571158China

A new species of the genus Gonyosoma Wagler,1828 is described herein based on six specimens from the Diaoluoshan Mountains,Hainan Island,Hainan Province,*** new species,Gonyosoma hainanense ***.,is most similar to its continental sister species,Gonyosoma boulengeri(Mocquard,1897).Both taxa have a scaled protrusion on the anterior portion of the rostrum,distinct from other ***,Gonyosoma hainanense *** be distinguished from *** by two significant morphological characters:(1)black orbital stripe absent in adults(*** in ***);and(2)two loreals(*** loreal in ***).The new species is also genetically divergent and forms a unique clade from its sister species and all other congeners based on sequences of the mitochondrial gene cytochrome b(cyt b).

关键词： Island Hainan sister

Relationship between symptom response and esophageal pH level on standard dose of esomeprazole treatment for gastroesophageal reflux disease

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Chinese Medical Journal 2010年第15期123卷 2012-2017页

作者： PENG Sui XIONG Li-shou XIAO Ying-lian WANG An-jiang LIN jin-kun HU Pin-jin chen min-hu Division of Gastroenterology & Hepatology the First Affiliated Hospital of Sun Yat-sen University Guangzhou Guangdong 510080 China

Background The relationship between symptom elimination and normalization of esophageal acid level of gastroesophageal reflux disease （GERD） on proton-pump inhibitor （PPI） therapy has been questioned. This study aimed to evaluate the relationship between symptom response and gastro-esophageal acidity control in Chinese patients with GERD on esomeprazole therapy, and to assess the role of 24-hour esophageal pH-metry after therapy in GERD *** GERD patients with typical reflux symptoms were enrolled and received esomeprazole 40 mg once daily for 4 weeks. Patients with positive baseline 24-hour esophageal pH-metry were divided into two groups depending on an additional dual-channel 24-hour pH-metry after treatment. The pH- group achieved normalization of esophageal pH level whereas the pH＋ group did *** Of the 80 patients studied, 76 had abnormal baseline esophageal pH levels. Of these, 90% （52/58） of symptom-free patients and 67% （12/18） of symptom-persistent patients achieved esophageal pH normalization after therapy （P=0.030）. The mean post-therapy gastric nocturnal percent time of pH 〈4.0 was significantly higher in pH＋group than that in pH- group （P 〈0.001） after therapy. The multivariate regression analysis identified hiatus hernia （P〈0.001） and persistent reflux symptom （P=0.004） were two independent factors predicting the low post-therapy esophageal pH *** Symptom elimination is not always accompanied by esophageal pH normalization, and vice *** pH-metry is recommended for GERD patients with hiatus hernia or with persistent reflux symptoms after PPI therapy.

关键词： gastroesophageal reflux esomeprazole symptom response esophageal pH level

Molecular phylogeny and morphological comparisons of the genus Hebius Thompson, 1913(Reptilia:Squamata: Colubridae) uncover a new taxon from Yunnan Province, China, and support revalidation of Hebius septemlineatus(Schmidt, 1925)

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Zoological Research 2021年第5期42卷 620-625页

作者： Shao-Bing Hou Zhi-Yong Yuan Ping-Fan Wei Gui-Gang Zhao Gao-Hui Liu Yun-He Wu Wen-jing Shen jin-min chen Peng Guo jing Che State Key Laboratory of Genetic Resources and Evolution State Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650223China Kunming College of Life Science University of the Chinese Academy of SciencesKunmingYunnan 650204China Key Laboratory for Conserving Wildlife with Small Populations in Yunnan Southwest Forestry UniversityKunmingYunnan 650224China Chinese Research Academy of Environmental Sciences Beijing 100012China Faculty of Agriculture Forest and Food EngineeringYibin UniversityYibinSichuan 644007China

We describe a new species of the genus Hebius and provide evidence for the validity of *** ***..Morphological and molecular analyses of Hebius specimens collected in Yunnan Province,China,revealed three distinct lineages,namely the newly described Hebius weixiensis ***.,as well as ***(Boulenger,1904),and *** ***.(Schmidt 1925),which is removed from synonymy with *** on mitochondrial genealogy,Hebius weixiensis *** sister to *** ***.,while *** is sister to *** new species and *** comb.

关键词： removed Morphological sister

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基于分形接触理论的结合面法向接触参数预估

上海交通大学学报 2011年第9期45卷 1275-1280页

作者：尤晋闽陈天宁西安交通大学机械工程学院西安710049

基于分形接触理论,建立了结合面法向接触参数的分形预估模型,通过粗糙表面材料性能参数、法向载荷及粗糙表面的分形参数来预估法向接触刚度和接触阻尼,并对其变化规律进行数值仿真.结果表明:结合面之间的接触处于弹性变形和与塑性变形... 详细信息

基于分形接触理论,建立了结合面法向接触参数的分形预估模型,通过粗糙表面材料性能参数、法向载荷及粗糙表面的分形参数来预估法向接触刚度和接触阻尼,并对其变化规律进行数值仿真.结果表明:结合面之间的接触处于弹性变形和与塑性变形共存的状态,且小接触点面积的微凸体发生塑性变形,而大接触点面积的微凸体发生弹性变形;法向接触参数与分形参数之间具有较强的非线性关系;同时,法向接触刚度随法向载荷的增大而逐渐增加,但法向载荷对结合面的法向阻尼特性影响较小;仿真结果中极值点的存在,为结合面接触参数的优化设计提供了依据.

关键词：结合面接触刚度接触阻尼预估分形模型

BENSON法预测标准生成焓的改进与程序实现

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计算机与应用化学 1999年第6期16卷 426-434页

作者：谭瑾陈敏伯郑崇直中国科学院上海有机化学研究所

本工作改进、扩充了Ｂｅｎｓｏｎ－Ｂｕｓｓ的基团加和法计算有机化合物标准生成焓的方案，完成了化学结构特征的计算机识别，扩充了Ｂｅｎｓｏｎ的碎片库，改良了旁式校正方法，建立了原子属性库、碎片库、环校正库、顺式校正... 详细信息

本工作改进、扩充了Ｂｅｎｓｏｎ－Ｂｕｓｓ的基团加和法计算有机化合物标准生成焓的方案，完成了化学结构特征的计算机识别，扩充了Ｂｅｎｓｏｎ的碎片库，改良了旁式校正方法，建立了原子属性库、碎片库、环校正库、顺式校正库。将该方法实现成微机上运行的Ｗｉｎｄｏｗｓ应用软件。对７０４个化合物的预测值与实验值进行相关性分析，相关性系数为０－９７５。改进后的Ｂｅｎｓｏｎ法比其它基团加和法有明显的优越性。

关键词：气态标准生成焓 Benson法基团加和法程序