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氢氯噻嗪联合厄贝沙坦治疗老年原发性高血压的效果及对血压与生活质量的影响

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临床合理用药杂志 2024年第21期17卷 21-24页

作者：齐吉芳毕鹏李飞兵江西省景德镇市中医医院心血管内科 333000

目的观察氢氯噻嗪联合厄贝沙坦治疗老年原发性高血压的效果及对患者血压与生活质量的影响。方法选取2022年5月—2023年5月景德镇市中医医院收治的老年原发性高血压患者76例,根据随机数字表法分为单独采用厄贝沙坦片治疗的厄贝沙坦组(n... 详细信息

目的观察氢氯噻嗪联合厄贝沙坦治疗老年原发性高血压的效果及对患者血压与生活质量的影响。方法选取2022年5月—2023年5月景德镇市中医医院收治的老年原发性高血压患者76例,根据随机数字表法分为单独采用厄贝沙坦片治疗的厄贝沙坦组(n=38)和采用氢氯噻嗪片联合厄贝沙坦片治疗的联合用药组(n=38)。2组均持续治疗3个月。比较2组患者的临床疗效,治疗前后血压、心率与血清N末端脑钠肽前体(NT-proBNP)、血钾水平及世界卫生组织生存质量量表简表评分,不良反应。结果联合用药组治疗总有效率为94.74%,高于厄贝沙坦组的73.68%(χ^(2)=6.333,P=0.012)。治疗3个月后,2组收缩压、舒张压、心率及血清NT-proBNP、血钾水平较治疗前均下降,且联合用药组低于厄贝沙坦组(P<0.01);2组社会、躯体、环境、心理和综合评分均升高,且联合用药组高于厄贝沙坦组(P<0.05或P<0.01)。联合用药组不良反应总发生率为7.89%,低于厄贝沙坦组的26.32%(χ^(2)=4.547,P=0.033)。结论老年原发性高血压患者采用氢氯噻嗪联合厄贝沙坦治疗效果显著,可明显降低患者血压,改善血清NT-proBNP和血钾水平,提高患者生活质量,且用药安全可靠。

关键词：原发性高血压老年人氢氯噻嗪厄贝沙坦血压生活质量

Clinicopathological significance and immunotherapeutic outcome of claudin 18.2 expression in advanced gastric cancer:A retrospective study

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Chinese Journal of Cancer Research 2024年第1期36卷 78-89页

作者： Changsong qi Xiaoyi Chong Ting Zhou Mingyang Ma jifang Gong Miao Zhang Jian Li Jun Xiao Xiaohui Peng Zhen Liu Zonghai Li Lin Shen Xiaotian Zhang State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers Beijing Key Laboratory of Carcinogenesis and Translational ResearchDepartment of Gastrointestinal OncologyPeking University Cancer Hospital&InstituteBeijing 100142China Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing) Department of Gastrointestinal OncologyPeking University Cancer Hospital&InstituteBeijing 100142China CARsgen Therapeutics Co. LtdShanghai 200231China CARsgen Life Sciences Co. LtdShanghai 200131China

Objective: Immunotherapeutic outcomes and clinical characteristics of claudin 18 isoform 2 positive(CLDN18.2-positive) gastric cancer(GC) vary in different clinical studies, making it difficult to optimize antiCLDN18.2 therapy. We conducted a retrospective analysis to explore the association of CLDN18.2 expression with clinicopathological characteristics and immunotherapeutic outcomes in ***: A total of 536 advanced GC patients from 2019 to 2021 in the CT041-CG4006 and CT041-ST-01clinical trials were included in the analysis. CLDN18.2 expression on ≥40% of tumor cells(2+, 40%) and CLDN18.2 expression on ≥70% of tumor cells(2+, 70%) were considered the two levels of positively expressed GC. The clinicopathological characteristics and immunotherapy outcomes of GC patients were analyzed according to CLDN18.2 expression ***: CLDN18.2 was expressed in 57.6%(cut-off: 2+, 40%) and 48.9%(cut-off: 2+, 70%) of *** death-ligand 1(PD-L1) and CLDN18.2 were co-expressed in 19.8% [combined positive score(CPS)≥1, CLDN18.2(cut-off: 2+, 40%)] and 17.2% [CPS≥5, CLDN18.2(cut-off: 2+, 70%)] of ***18.2 expression positively correlated with younger age, female sex, non-gastroesophageal junction(nonGEJ), and diffuse phenotype(P<0.001). HER2 and PD-L1 expression were significantly lower in CLDN18.2-positive GC(both P<0.05). Uterine adnexa metastasis(P<0.001) was more frequent and liver metastasis(P<0.001)was less common in CLDN18.2-positive GC. Overall survival and immunotherapy-related progression-free survival(ir PFS) were inferior in the CLDN18.2-positive ***: CLDN18.2-positive GC is associated with poor prognosis and worse immunotherapeutic outcomes. The combination of anti-CLDN18.2 therapy, anti-PD-L1/PD-1 therapy, and chemotherapy for GC requires further investigation.

关键词： Gastric cancer claudin18.2 CT041 PD-L1 immunotherapy

益生菌联合阿奇霉素治疗小儿急性肠胃炎临床疗效观察

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基层医学论坛 2017年第31期21卷 4274-4276页

作者：蒋小龙齐纪芳蠡县医院河北蠡县071400

目的观察益生菌联合阿奇霉素治疗小儿急性肠胃炎的临床效果。方法入选的54例小儿急性肠胃炎患者被随机分为治疗组和对照组各27例,治疗组采用益生菌联合阿奇霉素治疗,对照组仅采用阿奇霉素治疗,观察对比2组的治疗效果。结果 1个疗程后,... 详细信息

目的观察益生菌联合阿奇霉素治疗小儿急性肠胃炎的临床效果。方法入选的54例小儿急性肠胃炎患者被随机分为治疗组和对照组各27例,治疗组采用益生菌联合阿奇霉素治疗,对照组仅采用阿奇霉素治疗,观察对比2组的治疗效果。结果 1个疗程后,治疗组和对照组的总有效率分别为100%和74.01%;不良反应发生率分别为3.70%和37.04%,差异均有统计学意义(P<0.05)。结论益生菌联合阿奇霉素治疗小儿急性肠胃炎比单纯使用阿奇霉素临床效果好,不良反应少,更值得推广应用。

关键词：小儿急性肠胃炎益生菌阿奇霉素片联合用药

艺术想象、文化坚守与共同体价值的国际传达——对话动画电影《雪域少年》导演路奇

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当代动画 2024年第1期 42-47页

作者：路奇张娟杨继芳长春电影制片厂成都大学中国-东盟艺术学院影视与动画学院

张娟(以下简称张):您最早是什么时候产生了改编《格萨尔王传》这部藏族说唱体史诗的想法?为什么会选择用动画电影的方式来呈现?路奇(以下简称路):这是两个过程。36年前,我到西藏拍一部电影,借着工作的机会在藏区走了很多地方。当时,我... 详细信息

张娟(以下简称张):您最早是什么时候产生了改编《格萨尔王传》这部藏族说唱体史诗的想法?为什么会选择用动画电影的方式来呈现?路奇(以下简称路):这是两个过程。36年前,我到西藏拍一部电影,借着工作的机会在藏区走了很多地方。当时,我无意中看到了一张唐卡画,瞬间就被深深吸引。唐卡的画风比较独特:一是以线条为主,而且线条处理的方式非常细腻;二是色彩十分鲜艳、华丽。我找了一个当地人问,才知道画里是格萨尔王。也就是从那个时候开始了解到格萨尔王这个传奇故事,当时我就想什么时候一定要把它拍成电影。后来,我多次去西藏采风,特别喜欢那片神奇的土地.

关键词：格萨尔王艺术想象《格萨尔王传》文化坚守传奇故事动画电影唐卡画说唱体

Expression Analysis of the Insulin-Like Growth Factors Ⅰ and Ⅱ During Embryonic and Early Larval Development of Turbot(Scophthalmus maximus)

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Journal of Ocean University of China 2015年第2期14卷 309-316页

作者： WEN Haishen qi qian HU Jian SI Yufeng HE Feng LI jifang Fisheries College Ocean University of China

The insulin-like growth factors Ⅰ and Ⅱ （IGF-Ⅰ and IGF-Ⅱ） are important proteins involved in fish growth and develop- ment. Here, we report the isolation of IGF-Ⅱ and expression analysis of IGFs in turbot Scophthalmus maximus, aiming to clarify their function in embryonic and larval development of fish. The deduced IGF-Ⅱ gene is 808 bp in full length, which encodes a protein of 219 amino acids and is 93% similar with that ofParalichthys olicaceus in amino acid sequence. The tissue abundance and the ex- pression pattern of IGFs in a turbot at early development stages were investigated via reverse transcription-polymer chain reaction. Result showed that the IGF-Ⅰ and IGF-Ⅱ genes were widely expressed in tissues of S. maximus. IGF-Ⅰ was detected in all tissues ex- cept intestines with the highest level in liver, while IGF-Ⅱ transcript presented in all tissues except muscle. At the stages of embry- onic and larval development, the mRNA levels of IGFs sharply increased from the stage of unfertilized egg to post larva, followed by a decrease with larval development. However, there was an increase in IGF-Ⅰ at the embryonic stage and IGF-Ⅱ at the gastrula stage, respectively. These results suggested that IGFs play important roles in cell growth and division of the turbot. Our study provides reference data for further investigation of growth regulation in turbot, which can guarantee better understanding of the physiological role that IGFs play in fish.

关键词： Scophthalmus maximus insulin-like growth factor cloning expression analysis

Systematic Exploration of Optimized Base Editing gRNA Design and Pleiotropic Effects with BExplorer

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Genomics, Proteomics & Bioinformatics 2023年第6期21卷 1237-1245页

作者： Gongchen Zhang Chenyu Zhu Xiaohan Chen jifang Yan Dongyu Xue Zixuan Wei Guohui Chuai qi Liu Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji UniversityShanghai 200092China

Base editing technology is being increasingly applied in genome engineering,but the current strategy for designing guide RNAs(gRNAs)relies substantially on empirical experience rather than a dependable and efficient in silico ***,the pleiotropic effect of base editing on disease treatment remains unexplored,which prevents its further clinical ***,we presented BExplorer,an integrated and comprehensive computational pipeline to optimize the design of gRNAs for 26 existing types of base editors in *** BExplorer,we described its results for two types of mainstream base editors,BE3 and ABE7.10,and evaluated the pleiotropic effects of the corresponding base editing *** revealed 524 and 900 editable pathogenic single nucleotide polymorphism(SNP)loci in the human genome together with the selected optimized gRNAs for BE3 and ABE7.10,*** addition,the impact of 707 edited pathogenic SNP loci following base editing on 131 diseases was systematically explored by revealing their pleiotropic effects,indicating that base editing should be carefully utilized given the potential pleiotropic ***,the systematic exploration of optimized base editing gRNA design and the corresponding pleiotropic effects with BExplorer provides a computational basis for applying base editing in disease treatment.

关键词： Gene editing Base editing Pleiotropy CRISPR/Cas9 gRNAdesign

Characterization of Caspase Gene Family Members in Spotted Sea Bass(Lateolabrax maculatus)and Their Expression Profiles in Response to Vibrio harveyi Infection

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中国海洋大学学报:英文版 2023年第5期22卷 1370-1382页

作者： YANGLANG Arat WEN Haishen MAO Xuebin TIAN Yuan WANG Lingyu LI Jinku qi Xin SRISAPOOME Prapansak LI jifang LI Yun The Key Laboratory of Mariculture(Ocean University of China) Ministry of EducationQingdao 266003China Laboratory of Aquatic Animal Health Management Department of AquacultureFaculty of FisheriesKasetsart UniversityBangkok 10900Thailand

The caspase gene family is a crucial gene cluster that regulates apoptosis which contribute to programmed cell death,cell proliferation and differentiation,and several immune *** our study,a complete set of 12 caspase genes were identified in spotted sea bass Lateolabrax *** genes were divided into three subfamilies:2 inflammatory caspases(casp-1 and casp-14-like),5 apoptosis initiators(casp-2,casp-8a,casp-8b,casp-9,and casp-10),and 5 apoptosis executioners(casp-3a,casp-3b,casp-3-like,casp-6,and casp-7).Their phylogenetic relationships,synteny and gene structures were systematically ***,the relative expression profiles of the caspase family members in the liver,intestine,head kidney,and spleen were measured by q PCR after infection with Vibrio *** results showed that the overall mRNA levels of the caspase genes were dramatically increased after *** infection,and the expression patterns varied among genes and *** caspase genes underwent pronounced expression changes in the head kidney and spleen than in the liver or intestine,mainly after 48 h of the ***,casp-3a,casp-3b,casp-3-like,casp-6,casp-7,casp-8a,casp-8b,casp-10,and casp-14-like in the head kidney,and casp-3-like,casp-6,casp-7,and casp-14-like in the spleen,were the most responsive caspase genes which may contribute significantly to immune regulation in spotted sea ***,the apoptosis level in head kidney and spleen after infection were examined using the Caspase *** study provides a systemic overview of the caspase gene family in spotted sea bass after *** infection and lays a foundation for further deciphering the biological roles of these caspase genes.

关键词： caspase gene family spotted sea bass Vibrio harveyi gene expression apoptosis

Cloning and Expression Analysis of vasa During the Reproductive Cycle of Korean Rockfish, Sebastes schlegeli

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Journal of Ocean University of China 2013年第1期12卷 115-124页

作者： MU Weijie WEN Haishen HE Feng LI jifang LIU Miao MA Ruiqin ZHANG Yuanqing HU Jian qi Baoxia Fisheries College Ocean University of China College of Animal Science and Technology Inner Mongolia Nationality University

Vasa, which is a conserved member of the DEAD-box protein family, plays an indispensable role in primordial germ cell proliferation. However, the expression ofvasa gene during the reproductive cycle in ovoviviparous fish has not been documented. In this study, the full-length sequence of vasa was obtained from the ovary of Korean rockfish （Sebastes schlegeli） using reverse transcription-PCR and rapid amplification of cDNA ends. The Vasa with a mature protein of 650 amino acids showed greatest homology （84%） with giant gourami （Osphronemus gorarny） and Pacific bluefin tuna （Thunnus orientalis）. The expression of vasa mRNA in Korean rockfish was detected in gonads only, suggesting its specific role in gonadal development. In addition, seasonal changes in the vasa expression levels were examined in gonads by quantitative real-time PCR. The vasa transcript levels in adult testis were found higher during spermatogenesis than during spermiation. The vasa transcript levels remained relatively high at the early ovary stage but declined during ovary maturation in adult female fish. These results suggest that the vasa gene play an important role in spermatogenesis and early oogenesis during the reproductive cycle of Korean rockfish.

关键词： Korean rockfish vasa gene molecular cloning gene expression

有机硅密封胶与不同基材粘接的耐老化性能测试研究

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粘接 2024年第5期51卷 9-12页

作者：刘翀齐贝贝陈继芳杨晓菲朱强肖鹏张燕红中国特种飞行器研究所湖北荆门448000 郑州思蓝德新材料科技有限公司河南郑州450000

为提高有机硅密封胶用于不同基材粘接的老化性能,使用双组分有机硅密封胶制备不同粘接基材、不同胶层厚度的剪切试验件,测试密封胶在不同老化环境条件处理后的力学性能和粘接性能。结果表明,密封胶试验件力学性能和粘接性能在不同粘接... 详细信息

为提高有机硅密封胶用于不同基材粘接的老化性能,使用双组分有机硅密封胶制备不同粘接基材、不同胶层厚度的剪切试验件,测试密封胶在不同老化环境条件处理后的力学性能和粘接性能。结果表明,密封胶试验件力学性能和粘接性能在不同粘接基材、不同胶层厚度、不同老化环境下各有差异,双组分有机硅密封胶对所测基材均粘接良好;在设定厚度范围中,胶层厚度与剪切强度呈现负相关;在所测试老化环境中,不产生粘接破坏时,湿热老化对力学性能影响最大。

关键词：密封胶老化环境力学性能粘接性能