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3D genome organization in the central nervous system,implications for neuropsychological disorders

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Journal of Genetics and Genomics 2021年第12期48卷 1045-1056页

作者： Daijing Sun jie weng Yuhao Dong Yan Jiang Institutes of Brain Science State Key Laboratory of Medical NeurobiologyMOE Frontier Center for Brain ScienceFudan UniversityShanghai 200032China

Chromosomes in eukaryotic cell nuclei are highly compacted and finely organized into hierarchical threedimensional(3 D) configuration. In recent years, scientists have gained deeper understandings of 3 D genome structures and revealed novel evidence linking 3 D genome organization to various important cell events on the molecular level. Most importantly, alteration of 3 D genome architecture has emerged as an intriguing higher order mechanism that connects disease-related genetic variants in multiple heterogenous and polygenic neuropsychological disorders, delivering novel insights into the etiology. In this review, we provide a brief overview of the hierarchical structures of 3 D genome and two proposed regulatory models,loop extrusion and phase separation. We then focus on recent Hi-C data in the central nervous system and discuss 3 D genome alterations during normal brain development and in mature neurons. Most importantly,we make a comprehensive review on current knowledge and discuss the role of 3 D genome in multiple neuropsychological disorders, including schizophrenia, repeat expansion disorders, 22 q11 deletion syndrome, and others.

关键词： 3D genome Chromosome conformation Hi-C Central nervous system Neuropsychological disorders

Enhanced osteogenesis of hydroxyapatite scaffolds by coating with BMP-2-loaded short polylactide nanofiber: a new drug loading method for porous scaffolds

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Regenerative Biomaterials 2020年第1期7卷 91-98页

作者： Taotao Xu Luyao Sheng Lei He jie weng Ke Duan Key Laboratory of Advanced Technologies of Materials(MOE) School of Materials Science and EngineeringSouthwest Jiaotong UniversityChengdu 610031China Sichuan Provincial Laboratory of Orthopaedic Engineering Department of Bone and Joint SurgeryAffiliated Hospital of Southwest Medical UniversityLuzhou 646000China

Porous hydroxyapatite(HA)is widely used in porous forms to assist bone defect ***,further improvements in biological functions are desired for meeting complex clinical situations such as impaired bone regeneration in poor bone *** extracellular matrix(ECM)of human tissues is characterized by nanofibrous structures and a variety of signal *** these characteristics are expected to create a favorable microenvironment for cells and simultaneously allow release of osteogenic *** this study,short polylactide fibers containing BMP-2 were prepared by electrospinning and coated on porous HA *** coating did not affect porosity or pore interconnectivity of the scaffold but improved its compressive strength *** fiber coating produced burst BMP-2 release in 1 day followed by a linear release for 24 *** coating had a significantly lower rat calvarial osteoblasts(RCOBs)adhesion(*** scaffold)but allowed normal proliferation *** marrow stem cells(MSCs)on the coated scaffolds expressed a significantly increased alkaline phosphatase activity relative to the uncoated *** implantation in canine dorsal muscles,the coated scaffolds formed significantly more new bone at Weeks 4 and 12,and more blood vessels at Week *** method offers a new option for drug delivery systems.

关键词： short fibers surface modification drug release hydroxyapatite polylactide

Enhanced Adhesion of Mussel-inspired Adhesive through Manipulating Contents of Dopamine Methacrylamide and Molecular Weight of Polymer

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Journal of Bionic Engineering 2018年第3期15卷 461-470页

作者： Xiong Xiong Yumei Liu Feng Shi Guowei Zhang jie weng Shuxin Qu Key Lab of Advanced Technologies of Materials Ministry of Education School of Materials Science and Engineering Southwest Jiaotong University Chengdu 610031 China School of Life Science and Engineering Southwest Jiaotong University Chengdu 610031 China College of Environmental Science and Engineering China West Normal University Nanchong 637009 China Collaboration Innovation Center for Tissue Repair Material Engineering Technology China West Normal University Nanehong 637009 China

The aim of this study is to investigate the effect of the contents of modified 3, 4-dihydroxyphenyl-L-alanine （DOPA）, named as do- pamine methacrylamide （DMA）, on the adhesion of mussel-inspired adhesives in air and water. A series of adhesives, p（DMA-co-MEA）, were synthesized by copolymerized DMA and methoxy ethylacrylate （MEA） with the content of DMA from 2 mol.% to 10 mol.%. Results of IH NMR show that the contents of DMA in all adhesives are near to the theory ratios of DMA in the staring reagents. Adhesives with more than 5 mol.% of DMA appear adhesion, while adhesives with 2 mol. % and 3 mol. % of DMA show almost no adhesion in air and water. Adhesive with 7 mol.% of DMA has the highest molecular weight and adhesion either in air or in water in all adhesives. Adhesion of adhesive is synergistically influenced by the content of DMA, molecular weight and elastic modulus of adhesive. It is because that higher content of DMA would provide more DOPA, which leads to the coordination bond between DOPA and metal ions. It is feasible to develop the mussel-inspired adhesive through incorporating DMA into polymers, which will have potential application in the clinic.

关键词： mussel-inspired adhesive adhesion dopamine methacrylamide

The Drug-carrier Interactions, Release Behaviors and Cell Responses of Hydroxyapaptite Containing Several Chinese Medicines

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Journal of Wuhan University of Technology(Materials Science) 2013年第1期28卷 163-171页

作者：林孙忠屈树新 CHANG Lina GUO Yuehua DUAN Ke weng jie Key Lab of Advanced Technologies of Materials Ministry of Education School of Materials Science and Engineering Southwest Jiaotong University

The present work shows drug-carrier interactions, release behaviors and cell responses of hydroxyapatite （HA） containing salvianolic acid B （Sal B）, astragalus polysaecharide （APS）, and naringin. X-ray diffraction （XRD） showed that the crystallinity and crystal size of HA decreased significantly when Sal B was added （p〈0.05）. Transmission electron microscope （TEM） confirmed that the nano-acicular crystals of HA containing Sal B were the most fine among all specimens. It was conjectured that Sal B preferentially adsorbed on the positively charged surface of HA crystals to inhibit their growth. In vitro release of HA containing Chinese medicines followed the first-order equation. The drug-carrier affinity between HA and Sal B might have prolonged the release of Sal B. The proliferation and differentiation of osteoblasts were promoted by Chinese medicines containing HA in the time and dosage dependent manner. The osteoblasts displayed a polygonal morphology with cell-cell junctions in all cases. It is suggested that the contained Chinese medicines would promote the activities of the osteoblasts.

关键词： hydroxyapatite chinese medicines crystallinity crystal size drug release cell response

Anatomic resection of liver segments 6-8 for hepatocellular carcinoma

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World Journal of Gastroenterology 2014年第15期20卷 4433-4439页

作者： Chang-Ku Jia jie weng You-Ke Chen Yu Fu Department of Hepatobiliary Pancreatic Surgery the Affiliated Hospital of Hainan Medical College

AIM: To report the devised anatomic liver resection of segments 6, 7 and 8 to improve the resection rate for patients with right liver tumors.

关键词： Anatomic hepatectomy Hepatocellular carcinoma Selective occlusion Alpha-fetoprotein Liver tumor

The effect of macropore size of hydroxyapatite scaffold on the osteogenic differentiation of bone mesenchymal stem cells under perfusion culture

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Regenerative Biomaterials 2021年第6期8卷 80-91页

作者： Feng Shi Dongqin Xiao Chengdong Zhang Wei Zhi Yumei Liu jie weng Collaboration Innovation Center for Tissue Repair Material Engineering Technology College of Life ScienceChina West Normal UniversityNo.1 Shida RoadNanchongSichuan 637002China Research Institute of Tissue Engineering and Stem Cells Nanchong Central Hospitalthe Second Clinical College of North Sichuan Medical CollegeNo.97 Renmin South RoadNanchongSichuan 637000China College of Medicine Southwest Jiaotong UniversityNo.111 North 1st Section of Second Ring RoadChengduSichuan 610031China College of Environmental Science and Engineering China West Normal UniversityNo.1 Shida RoadNanchongSichuan 637002China

Previous studies have proved that dynamic culture could facilitate nutrients transport and apply mechanical stimulation to the cells within three-dimensional scaffolds,thus enhancing the differentiation of stem cells towards the osteogenic ***,the effects of macropore size on osteogenic differentiation of stem cells under dynamic condition are still ***,the objective of this study was to investigate the effects of macropore size of hydroxyapatite(HAp)scaffolds on osteogenic differentiation of bone mesenchymal stem cells under static and perfusion culture *** vitro cell culture results showed that cell proliferation,alkaline phosphate(ALP)activity,mRNA expression of ALP,collagen-I(Col-I),osteocalcin(OCN)and osteopontin(OPN)were enhanced when cultured under perfusion condition in comparison to static *** perfusion culture condition,the ALP activity and the gene expression of ALP,Col-I,OCN and OPN were enhanced with the macropore size decreasing from 1300 to 800 ***,with the further decrease in macropore size from 800 to 500 mm,the osteogenic related gene expression and protein secretion were *** fluid dynamics analysis showed that the distribution areas of medium-and high-speed flow increased with the decrease in macropore size,accompanied by the increase of the fluid shear stress within the *** results confirm the effects of macropore size on fluid flow stimuli and cell differentiation,and also help optimize the macropore size of HAp scaffolds for bone tissue engineering.

关键词： macropore size HAp scaffolds perfusion culture osteogenic differentiation

A High-Accuracy Curve Boundary Recognition Method Based on the Lattice Boltzmann Method and Immersed Moving Boundary Method

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Computer Modeling in Engineering & Sciences 2024年第9期140卷 2533-2557页

作者： jie-Di weng Yong-Zheng Jiang Long-Chao Chen Xu Zhang Guan-Yong Zhang Hunan Provincial Key Laboratory of Health Maintenance for Mechanical Equipment Hunan University of Science and TechnologyXiangtan411201China School of Mechanical Engineering Hunan Chemical Vocational Technology CollegeZhuzhou412000China

Applying numerical simulation technology to investigate fluid-solid interaction involving complex curved bound-aries is vital in aircraft design,ocean,and construction ***,current methods such as Lattice Boltzmann(LBM)and the immersion boundary method based on solid ratio(IMB)have limitations in identifying custom curved ***,IBM based on velocity correction(IBM-VC)suffers from inaccuracies and numerical ***,this study introduces a high-accuracy curve boundary recognition method(IMB-CB),which identifies boundary nodes by moving the search box,and corrects the weighting function in LBM by calculating the solid ratio of the boundary nodes,achieving accurate recognition of custom curve *** addition,curve boundary image and dot methods are utilized to verify *** findings revealed that IMB-CB can accurately identify the boundary,showing an error of less than 1.8%with 500 ***,the flow in the custom curve boundary and aerodynamic characteristics of the NACA0012 airfoil are calculated and compared to *** showed that IMB-CB yields lower lift and drag coefficient errors than IBM-VC,with a 1.45%drag coefficient *** addition,the characteristic curve of IMB-CB is very stable,whereas that of IBM-VC is *** the moving boundary problem,LBM-IMB-CB with discrete element method(DEM)is capable of accurately simulating the physical phenomena of multi-moving particle flow in complex curved *** research proposes a new curve boundary recognition method,which can significantly promote the stability and accuracy of fluid-solid interaction simulations and thus has huge applications in engineering.

关键词： Fluid-solid interaction curve boundary recognition method Lattice Boltzmann method immersed moving boundary method

Ectopic osteogenesis and angiogenesis regulated by porous architecture of hydroxyapatite scaffolds with similar interconnecting structure in vivo

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Regenerative Biomaterials 2016年第5期3卷 285-297页

作者： Jinyu Li Wei Zhi Taotao Xu Feng Shi Ke Duan JianxinWang Yandong Mu jie weng Key Laboratory of Advanced Technologies of Materials(MOE) School of Materials Science and EngineeringSouthwest Jiaotong UniversityChengdu 610031China and 2Dental Department Sichuan Province People’s HospitalChengdu 610072People’s Republic of China

The macro-pore sizes of porous scaffold play a key role for regulating ectopic osteogenesis and angiogenesis but many researches ignored the influence of interconnection between macro-pores with different *** order to accurately reveal the relationship between ectopic osteogenesis and macro-pore sizes in dorsal muscle and abdominal cavities of dogs,hydroxyapatite(HA)scaffolds with three different macro-pore sizes of 500–650,750–900 and 1100–1250 mm were prepared via sugar spheres-leaching process,which also had similar interconnecting structure determined by keeping the d/s ratio of interconnecting window diameter to macro-pore size *** permeability test showed that the seepage flow of fluid through the porous scaffolds increased with the increase of macro-pore *** cell growth in three scaffolds was not affected by the macro-pore *** in vivo ectopic implantation results indicated that the macro-pore sizes of HA scaffolds with the similar interconnecting structure have impact not only the speed of osteogenesis and angiogenesis but also the space distribution of newly formed *** scaffold with macro-pore sizes of 750–900 mm exhibited much faster angiogenesis and osteogenesis,and much more uniformly distribution of new bone than those with othermacro-pore *** work illustrates the importance of a suitable macro-pore sizes in HA scaffolds with the similar interconnecting structure which provides the environment for ectopic osteogenesis and angiogenesis.

关键词： hydroxyapatite scaffolds similar interconnecting structure osteogenesis angiogenesis

New alkylamides from pericarps of Zanthoxylum bungeanum

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Chinese Chemical Letters 2012年第11期23卷 1247-1250页

作者： Shuai Huang Lei Zhao Xian Li Zhou Mei Ying Cui Juan Wang jie weng School of Life Science and Engineering Southwest Jiaotong UniversityChengdu 610031SichuanChina Food and Agriculture Standardization Institute China National Institute of StandardizationBeijing 100088China

Two new unsaturated alkylamides （2E,7E,9E）-N-（2-hydroxy-2-methylpropyl）-6,11-dioxo-2,7,9-dodecatrienamide （1） and （2E,6E,8E）-N-（2-hydroxy-2-methylpropyl）-10-oxo-2,6,8-decatrienamide （2） together with six known compounds （3-8） have been isolated from the pericarps of Zanthoxylum bungeanum. Their structures were elucidated on the basis of extensive spectroscopic analysis, including MS, IR, 1D and 2D NMR experiments.

关键词： Hua Jiao Zanthoxylum bungeanum Unsaturated alkylamides