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Inhibition effects of perfluoroalkyl acids on progesterone production in mLTC-1

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Journal of Environmental Sciences 2017年第6期29卷 272-280页

作者： Wei Zhao Ruina Cui Jianshe Wang jiayin dai Key Laboratory of Animal Ecology and Conservation Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China

Perfluoroalkyl substances （PFASs） are a class of fluorine substituted carboxylic acid, sulfonic acid and alcohol, structurally similar to their corresponding parent compounds. Previous study demonstrated the potential endocrine disruption and reproductive toxicity of perfluorooctane sulfonic acid and perfluorooctanoic acid, two dominant PFASs in animals and humans. We explored the relationship between eleven perfluoroalkyl acids （PFAAs） with different carbon chain length and their ability to inhibit progesterone production in mouse Leydig tumor cells （mLTC-1）. We found an obvious dose-response relationship between progesterone inhibition rate and PFAA exposure concentration in mLTC-1. The relative inhibition rate of progesterone by PFAAs was linearly related to the carbon chain length and molar refractivity of PFAAs. Mitochondrial membrane potential （MMP） decreased after PFAA exposure at the half-maximal inhibitory effect concentration （IC50） of progesterone production in mLTC-1, while the reactive oxygen species （ROS） content increased significantly. These results imply that the inhibition effect of PFAAs on progesterone production might be due, in part, to ROS damage and the decrease in MMP in mLTC-1.

关键词： Perfluoroalkyl substancesProgesteroneMitochondrial membrane potentialReactive oxygen species

Toxic effects of perfluorononanoic acid on the development of Zebrafish(Danio rerio) embryos

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Journal of Environmental Sciences 2015年第6期27卷 26-34页

作者： Hui Liu Nan Sheng Wei Zhang jiayin dai Key Laboratory of Animal Ecology and Conservation Biology Institute of Zoology Chinese Academy of Sciences University of Chinese Academy of Sciences

Perfluorononanoic acid（PFNA） is a nine-carbon perfluoroalkyl acid widely used in industrial and domestic products. It is a persistent organic pollutant found in the environment as well as in the tissues of humans and wildlife. There is a concern that this chemical might be a developmental toxicant and teratogen in various ecosystems. In the present study,the toxic effects of PFNA were evaluated in zebrafish（Danio rerio） embryos. One hour post-fertilization embryos were treated with 0, 25, 50, 100, 200, 300, 350, and 400 μmol/L PFNA for 96 hr in 6-well plates. Developmental phenotypes and hatching rates were observed and recorded. Nineteen genes related to oxidative stress and lipid metabolism were examined using Quantitative RT-PCR and confirmed by whole mount in situ hybridization（WISH）. Results showed that PFNA delayed the development of zebrafish embryos, reduced the hatching rate, and caused ventricular edema and malformation of the spine. In addition, the amount of reactive oxygen species in the embryo bodies increased significantly after exposure to PFNA compared with that of the control group. The Quantitative RT-PCR and WISH experiments demonstrated that m RNA expression of the lfabp and ucp2 genes increased significantly while that of sod1 and mt-nd1 decreased significantly after PFNA exposure. The m RNA expression levels of gpx1 and mt-atp6 decreased significantly in the high concentration group. However, the m RNA expression levels of both ppara and pparg did not show any significant variation after exposure. These findings suggest that PFNA affected the development of zebrafish embryos at relatively low concentrations.

关键词： Perfluorononanoic acid Zebrafish embryo Danio rerio Developmental toxicity

Effects of pentachlorophenol on the detoxification system in white-rumped munia(Lonchura striata)

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Journal of Environmental Sciences 2016年第6期28卷 224-234页

作者： Peng Jiang Jianshe Wang Jinguo Zhang jiayin dai Key Laboratory of Animal Ecology and Conservation Biology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China Beijing Zoo Beijing 100044 China

Pentachlorophenol（PCP）, a priority pollutant due to its persistence and high toxicity, has been used worldwide as a pesticide and biocide. To understand the adverse effects of PCP, adult male white-rumped munias（Lonchura striata） were orally administrated commercial PCP mixed with corn oil at dosages of 0, 0.05, 0.5, and 5 mg/（kg·day） for 42 day. Gas chromatography–mass spectrometry（GC–MS） analysis found that PCP was preferentially accumulated in the kidney rather than in the liver and muscle in all exposure groups. To examine the function of CYP1A in pollutant metabolism, we isolated two full-length cDNA fragments（designated as CYP1A4 and CYP1A5） from L. striata liver using reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends. PCP induced the expression of CYP1A5, although no obvious change was observed in CYP1A4 expression. Furthermore, PCP significantly elevated the activities of ethoxyresorufin O-deethylase and methoxyresorufin O-demethylase and decreased the activity of benzyloxy-trifluoromethyl-coumarin, with no significant responses observed in benzyloxyresorufin O-debenzylase. PCP induced significant changes in antioxidant enzyme（superoxide dismutase and catalase） activities and malondialdehyde content, but decreased glutathione peroxidase（GSH-Px） and glutathione S-transferase activities and GSH content in the liver of L. striata. The present study demonstrated that PCP had hepatic toxic effects by affecting CYP1As and anti-oxidative status.

关键词： CYP1A4 CYP1A5 Alkoxyresorufin O-dealkylase Antioxidant enzyme Lonchura striata

Distribution of polybrominated diphenyl ethers and decabromodiphenylethane in surface sediments from Fuhe River and Baiyangdian Lake,North China

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Journal of Environmental Sciences 2010年第12期22卷 1833-1839页

作者： Guocheng Hu Zhencheng Xu jiayin dai Bixian Mai Hong Cao Jianshe Wang Zhimin Shi Muqi Xu Metropolitan Environment Center South China Institute of Environmental Sciences Ministry of Environmental Protection Guangzhou 510655 China Key Laboratory of Animal Ecology and Conservation Biology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China State Key Laboratory of Organic Chemistry Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou 510640 China

Nineteen surface sediment samples collected from Baiyangdian Lake and its inflowing river （Fuhe River） in North China were analyzed for polybrominated diphenyl ethers （PBDEs） and decabromodiphenylethane （DBDPE）. The concentrations of PBDEs and DBDPE in sediments ranged from 5.5 to 300.7 ng/g dry weight （dw） and 1.1 to 68.2 ng/g dw, respectively. Their levels in sediments in Fuhe River were significantly higher than those in Balyandian Lake. Compared to data from other regions, the PBDE levels in surface sediments from Baiyangdian Lake and Fuhe River were in the medium to lower range. Among the PBDE congeners, BDE209 was predominant, with contributions to the total PBDEs ranging from 79.4% to 97.3% in sediment samples. For the lowly brominated congeners （tri- to hepta-BDE）, BDE47 and BDE99 were the most abundant, which contributed 52.1% and 44.1% to the sum of tri- to hepta-BDEs in the sediments from Baiyangdian Lake and Fuhe River, respectively. The compositional patterns of PBDEs in Baiyangdian Lake sediments indicated that technical deca-BDE mixture was the major pollutant sources with a minor contribution of penta-BDE mixture. The present study suggested that the importance of Fuhe River as a possibly potential sources of PBDEs contamination in Baiyangdian Lake.

关键词： polybrominated diphenyl ethers decabromodiphenylethane contamination profile Baiyangdian Lake Fuhe River

Organochlorine compounds and polycyclic aromatic hydrocarbons in surface sediment from Baiyangdian Lake,North China:Concentrations,sources profiles and potential risk

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Journal of Environmental Sciences 2010年第2期22卷 176-183页

作者： Guocheng Hu Xiaojun Luo Fengchao Li jiayin dai Jianyang Guo Shejun Chen Cao Hong Bixian Mai Muqi Xu Key Laboratory of Animal Ecology and Conservation Biology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China. State Key Laboratory of Organic Chemistry Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou 510640 China College of Science Life Hebei University Baoding 071002 China State Key Laboratory of Environmental Geochemistry Institute of Geochemistry Chinese Academy of Sciences Guiyang 55002 China

Organochlorine pesticides （OCPs）, polychlorinated biphenyls （PCBs）, and polycyclic aromatic hydrocarbons （PAHs） were determined in nineteen surface sediment samples collected from Baiyangdian Lake and its inflowing river （Fuhe River） in North China. Total concentrations of OCPs, PCBs and PAHs in sediments ranged from 5.4 to 707.6 ng]g, 2.3 to 197.8 ng/g, and 101.3 to 6360.5 ng]g, respectively. The levels of contaminants in Fuhe River were significantly higher than those in Baiyandian Lake. For hexachlorocyclohexane （HCHs） and dichlorodiphenytrich/oroethanes （DDTs）, α-HCH and p,p＇-DDT were predominant isomers; while for PCBs, PCB 28/31, PCB 40/103, PCB 60, PCB 101, and PCB 118 were predominant congeners. Possible sources derived from historical usage for OCPs and incomplete combustion fuel, wood, and coal and exhaustion of boats or cars for PAHs. Risk assessment of sediment indicated that sediments in Fuhe River were likely to pose potential biological adverse impact.

关键词： persistent organic pollutants concentrations profiles risk assessment Baiyangdian Lake

Dietary exposure to di-isobutyl phthalate increases urinary 5-methyl-2′-deoxycytidine level and affects reproductive function in adult male mice

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Journal of Environmental Sciences 2017年第11期29卷 14-23页

作者： Yitao Pan Xiaoyang Wang Leo W.Y.Yeung Nan Sheng Qianqian Cui Ruina Cui Hongxia Zhang jiayin dai Key Laboratory of Animal Ecology and Conservation Biology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China Man-Technology-Environment Research Centre (MTM) School of Science and Technology Orebro University Orebro SE -70182 Sweden

Phthalates are a large family of ubiquitous environmental pollutants suspected of being endocrine disruptors. Epidemiological studies have associated phthalate metabolites with decreased reproductive parameters and linked phthalate exposure with the level of urinary 5-methyl-2′-deoxycytidine（5mdC, a product of methylated DNA）. In this study, adult male mice were exposed to 450 mg di-isobutyl phthalate（DiBP）/（kg·day） via dietary exposure for 28 days. Mono-isobutyl phthalate（Mi BP, the urinary metabolite） and reproductive function parameters were determined. The levels of 5mdC and 5-hydroxymethyl-2′-deoxycytidine（5hmdC） were measured in urine to evaluate if their contents were also altered by DiBP exposure in this animal model. Results showed that DiBP exposure led to a significant increase in the urinary 5mdC level and significant decreases in sperm concentration and motility in the epididymis, accompanied with reduced testosterone levels and downregulation of the P450 cholesterol side-chain cleavage enzyme（P450scc） gene in the mice testes. Our findings indicated that exposure to DiBP increased the urinary 5mdC levels,which supported our recent epidemiological study about the associations of urinary 5mdC with phthalate exposure in the male human population. In addition, DiBP exposure impaired male reproductive function, possibly by disturbing testosterone levels; P450scc might be a major steroidogenic enzyme targeted by DiBP or other phthalates.

关键词： Di-isobutyl phthalate(DiBP) 'Reproductive function' 5-Methyl-2′-deoxycytidine(5mdC) 'Testosterone 'Deoxyribonucleic acid(DNA) methylation

新型全氟及多氟聚醚羧酸识别、分布特征及毒理效应研究进展

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科学通报 2024年第6期69卷 774-786页

作者：来冠铮戴家银盛南上海交通大学环境科学与工程学院生态环境部新型污染物环境健康影响评价重点实验室上海200240

随着全氟辛酸(PFOA)的管控,工业中开始使用结构中含有醚氧键的全氟及多氟聚醚羧酸(PFECA)作为加工助剂替代PFOA.近年来,得益于非靶向筛查和靶向分析技术的快速发展,不同环境介质和生物样品中已陆续检出多种PFECA,浓度呈升高趋势.已有研... 详细信息

随着全氟辛酸(PFOA)的管控,工业中开始使用结构中含有醚氧键的全氟及多氟聚醚羧酸(PFECA)作为加工助剂替代PFOA.近年来,得益于非靶向筛查和靶向分析技术的快速发展,不同环境介质和生物样品中已陆续检出多种PFECA,浓度呈升高趋势.已有研究发现,六氟环氧丙烷三聚体(HFPO-TA)、六氟环氧丙烷四聚体(HFPO-TeA)等PFECA表现出较PFOA更强的生物累积和毒性效应.相较于传统全氟及多氟烷基物质(PFAS),PFECA结构更为复杂,对环境和生物体的危害机制也可能存在差异.本文围绕PFECA的结构种类、污染水平和毒性效应,从非靶向识别、环境行为、生物及人群暴露水平、毒性效应和分子机制等多个方面对近年来的相关研究进行概述,探讨当前PFECA应用中存在的问题和潜在风险,对未来的研究方向和应用前景进行展望,旨在为PFAS替代品的环境污染及风险评估提供参考,为我国开展PFAS的管控和削减行动提供支撑.

关键词： PFECA 非靶向识别环境行为人体暴露毒性效应

全氟及多氟类化合物在人体分布及其毒性研究进展

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生态毒理学报 2013年第5期8卷 650-657页

作者：胡佳玥戴家银中国科学院动物研究所中国科学院动物生态与保护生物学重点实验室北京100101

全氟和多氟烷酸类化合物(perfluoroalkyl and polyfluoroalkyl substances,PFASs)是一类新型持久性有机污染物,目前其在民用和工业产品中得到广泛应用。在环境样品、野生动物以及人类体内都检测到了这类物质。PFASs对哺乳动物和水生生... 详细信息

全氟和多氟烷酸类化合物(perfluoroalkyl and polyfluoroalkyl substances,PFASs)是一类新型持久性有机污染物,目前其在民用和工业产品中得到广泛应用。在环境样品、野生动物以及人类体内都检测到了这类物质。PFASs对哺乳动物和水生生物具有广泛的毒性,其对生态环境和人体健康的影响受到公众关注。目前,PFASs对人类健康的研究还多停留在流行病学研究和体外细胞毒性研究方面,由于人类有复杂的免疫和代谢系统,因此PPASs对人类健康影响的具体机制和安全剂量仍然需要进一步研究。本文针对PFASs在人体的分布、流行病学研究、毒理学效应和作用机制等热点问题进行了总结,并对目前PFASs研究存在的问题及今后的研究方向进行讨论和展望。

关键词：全氟和多氟烷酸类化合物人类健康流行病学研究毒理学效应

物种敏感度分布法推导全氟辛酸和全氟辛烷磺酸水质生态风险阈值

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海洋环境科学 2024年第6期43卷 945-953,962页

作者：王晨羽潘奕陶戴家银上海交通大学环境科学与工程学院上海200240

我国尚无针对全氟及多氟烷基化合物(per-and polyfluoroalkyl substances,PFAS)的生态环境基准。本研究搜集了代表性化合物全氟辛烷磺酸(perfluorooctane sulfonate,PFOS)和全氟辛酸(perfluorooctanoic acid,PFOA)的水生急慢性毒性数据,基于物种敏感度分布(species sensitivity distribution,SSD)法推导海水和淡水生态风险阈值。其中,PFOA和PFOS的海水长期风险阈值分别为0.425μg/L和3.472μg/L,淡水阈值分别为13.43μg/L和1.822μg/L,暗示PFOA对海洋物种的毒性高于淡水物种,而相比于海洋物种,隆线溞(Daphnia carinata)等淡水物种可能对PFOS更为敏感。基于推导阈值对我国主要近岸海域进行风险评价,结果表明,渤海莱州湾存在PFOA潜在中风险区域,PFOS在我国海域无明显生态风险。研究结果可为PFAS水质基准制定和污染控制决策提供依据和参考。

关键词： PFOA PFOS 物种敏感度分布法生态风险评价