Inhibition effects of perfluoroalkyl acids on progesterone production in mLTC-1

作     者：Wei Zhao Ruina Cui Jianshe Wang Jiayin Dai 

作者机构：Key Laboratory of Animal Ecology and Conservation BiologyInstitute of ZoologyChinese Academy of SciencesBeijing 100101China 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2017年第29卷第6期

页      面：272-280页

核心收录：

学科分类：083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 08[工学] 09[农学] 0903[农学-农业资源与环境] 0713[理学-生态学] 

基　　金：supported by the National Basic Research Program(973)of China(No.2013CB945004) 

主　　题：Perfluoroalkyl substancesProgesteroneMitochondrial membrane potentialReactive oxygen species 

摘      要：Perfluoroalkyl substances （PFASs） are a class of fluorine substituted carboxylic acid, sulfonic acid and alcohol, structurally similar to their corresponding parent compounds. Previous study demonstrated the potential endocrine disruption and reproductive toxicity of perfluorooctane sulfonic acid and perfluorooctanoic acid, two dominant PFASs in animals and humans. We explored the relationship between eleven perfluoroalkyl acids （PFAAs） with different carbon chain length and their ability to inhibit progesterone production in mouse Leydig tumor cells （mLTC-1）. We found an obvious dose-response relationship between progesterone inhibition rate and PFAA exposure concentration in mLTC-1. The relative inhibition rate of progesterone by PFAAs was linearly related to the carbon chain length and molar refractivity of PFAAs. Mitochondrial membrane potential （MMP） decreased after PFAA exposure at the half-maximal inhibitory effect concentration （IC50） of progesterone production in mLTC-1, while the reactive oxygen species （ROS） content increased significantly. These results imply that the inhibition effect of PFAAs on progesterone production might be due, in part, to ROS damage and the decrease in MMP in mLTC-1.