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Current therapies for osteoarthritis and prospects of CRISPR-based genome,epigenome,and RNA editing in osteoarthritis treatment

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Journal of Genetics and Genomics 2024年第2期51卷 159-183页

作者： Yuxi Chen Xiao Luo Rui Kang Kaixin Cui jianping ou Xiya Zhang Puping Liang MOE Key Laboratory of Gene Function and Regulation State Key Laboratory of BiocontrolSchool of Life SciencesSun Yat-sen UniversityGuangzhouGuangdong 510275China Center for Reproductive Medicine The Third Affiliated Hospital of Sun Yat-sen UniversitySun Yat-sen UniversityGuangzhouGuangdong 510630China

Osteoarthritis(OA)is one of the most common degenerative joint diseases worldwide,causing pain,disability,and decreased quality of *** balance between regeneration and inflammation-induced degradation results in multiple etiologies and complex pathogenesis of ***,there is a lack of effective therapeutic strategies for OA *** the development of CRISPR-based genome,epigenome,and RNA editing tools,OA treatment has been improved by targeting genetic risk factors,activating chondrogenic elements,and modulating inflammatory *** by cell therapy and in vivo delivery vectors,genome,epigenome,and RNA editing tools may provide a promising approach for personalized OA *** review summarizes CRISPR-based genome,epigenome,and RNA editing tools that can be applied to the treatment of OA and provides insights into the development of CRISPR-based therapeutics for OA ***,in-depth evaluations of the efficacy and safety of these tools in human OA treatment are needed.

关键词： Osteoarthritis Genome editing Epigenone editing RNA editing

“The Colors of Water”:Miao Xiaolong's Modern Dance Concert

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China & The World Cultural Exchange 2007年第3期73卷 28-30页

作者： ou jianping

In China’s dancing arena today where most dancers pursue hasty success, it is truly rare to prepare a dance concert for as long as seven years. And more amazingly, the whole production is planned and presented by merely one person who choreographs and directs the dance concert, designs all costumes, stage settings and lighting as well

关键词：上海师大音乐学院舞蹈系苗小龙现代舞专场音乐会《水色》

Ballet Division Graces Tongji University at Its 100^(th) Anniversary

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China & The World Cultural Exchange 2007年第7期73卷 33-35页

作者： ou jianping

Aballet evening entitled “Three-color Trilogy” was presented by students from the Dance Division, the School of Film, Tongji University in the 2800-seat auditorium of the university on May 28, bringing a successful ... 详细信息

关键词：芭蕾舞同济大学纪念日文化

THE STORY OF LIN HWAI-MIN AND HIS CLouD GATE DANCE THEATRE

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China & The World Cultural Exchange 2007年第9期73卷 22-24页

作者： ou jianping

The Forum of Chinese Modern Dance took place in Beijing in July, attracting cultural celebrities, scholars and dancers from China's Mainland, Hong Kong

关键词：中国现代舞舞蹈艺术舞蹈工作室

ON q-WIENER INDEX OF UNICYCLIC GRAPHS

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Annals of Applied Mathematics 2018年第3期34卷 263-268页

作者： Xing Feng jianping ou Shaihua Liu Faculty of Science Jiangxi University of Science and Technology Department of Math. Wuyi UniversityJiangmen

The q-Wiener index of unicyclic graphs are determined in this work. As an example of its applications, an explicit expression of q-Wiener index of caterpillar cycles is presented.

关键词： q-Wiener index unicyclic graphs caterpillar cycles

Comparison of Pregnancy outcomes of High-Quality D5- and D6-Blastocyst Transfer in Hormone-Replacement Frozen-Thawed Cycles

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International Journal of Clinical Medicine 2017年第11期8卷 565-571页

作者： Weijie Xing Liuhong Cai Li Sun jianping ou Center for Reproductive Medicine The Third Affiliated Hospital of Sun Yat-sen University Guangzhou China

This study aimed to assess pregnancy outcomes after high-quality D5- and D6-blastocyst transfer in frozen cycles of in vitro fertilization and embryo transfer and to further evaluate whether there was a difference in blastocyst development potentials with different developmental speeds and in pregnancy outcomes. A retrospective analysis was conducted to analyze 247 frozen cycles in our center from September 2015 to July 2017, which were divided into two groups: a D5-FET group with 193 cycles of D5-blastocyst transfer, and a D6-FET group with 54 cycles of D6-blastocyst transfer. Hormone replacement method was utilized to prepare frozen-cycle endometria. Pregnancy outcomes were analyzed and compared between these two groups. The mean ages of the two groups were 31.45 ± 4.43 years and 31.98 ± 4.84 years, respectively, with no statistically significant differences (P > 0.05). The difference in the endometrial thickness during transfer was also not statistically significant. The implantation rate in the D5-FET group was 60.13%, significantly higher than that in the D6-FET group (31.58%, P P ound in the abortion rate and ectopic pregnancy rate between the two groups. The implantation, biochemical pregnancy, and clinical pregnancy rates of the blastocyst D5 were all superior to those of the blastocyst D6. In clinics, therefore, D5-blastocyst transfer could be prioritized for embryo transfer.

关键词： Frozen Cycle Hormone Replacement Therapy Blastocyst Transfer

Double-Lumen Needle Follicular Flushing System versus Single-Lumen Aspiration Needle in IVF/ICSI Patients with Poor Ovarian Response: A Meta-Analysis

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Advances in Sexual Medicine 2017年第4期7卷 167-178页

作者： Weijie Xing jianping ou Liuhong Cai Xin Tao Center for Reproductive Medicine The Third Affiliated Hospital of Sun Yat-sen University Guangzhou China

Background: The present study performed a meta-analysis to comprehensively analyze existing randomized controlled trials (RCT) involving the use of double-lumen needle in patients with poor ovarian response to explore whether double-lumen needle was good for specific patients. Methods: The PubMed, EMBASE, Cochrane Library databases and two randomized controlled trials registration centers were thoroughly searched until April 2017. The clinical outcomes of IVF/ICSI cycles were compared between two groups with double-lumen needle and single-lumen needle. Results: Four RCT studies were included in this present meta-analysis. The oocytes yield was similar in two groups (OR 0.88, 95%CI 0.66 - 1.16;I2 = 4%). The procedure time with double-lumen needle was significantly longer than that with single-lumen needle (IV = 1.98, 95%CI 0.95 - 3.00;I2 = 86%). The fertilization rate with double-lumen needle was lower than that with single-lumen needle (OR 0.66, 95%CI 0.44 - 0.97;I2 = 0%). There was no significant difference of live birth rate in two groups (OR 0.76, 95%CI 0.32 - 1.76;I2 = 41%). Conclusion: Double-lumen needle could not benefit patients with a POR in terms of the number of oocytes retrieved, oocyte recovery rate, normal fertilization rate, clinical pregnancy rate, and live birth rate, compared with single-lumen needle.

关键词： Double-Lumen Needle Single-Lumen Needle Poor Ovarian Response

Frozen Blastocyst Transfer during <i>in Vitro</i>Fertilization/Intracytoplasmic Sperm Injection Cycle Lowers the Rate of Ectopic Pregnancy

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Open Journal of Obstetrics and Gynecology 2017年第12期7卷 1183-1188页

作者： Weijie Xing Liuhong Cai Li Sun jianping ou Center for Reproductive Medicine The Third Affiliated Hospital of Sun Yat-sen University Guangzhou China

This study aimed to determine whether frozen blastocyst transfer can lower the incidence rate of ectopic pregnancy. A total of 1577 in vitro fertilization/ intra-cytoplasmic cycles were analyzed, including 757 fresh D3-ET cycles, 429 frozen D3-ET cycles, and 391 frozen D5-ET cycles. The differences of ages, the proportion of IVF cycles, the proportion of primary infertility, and the proportion of tubal factor between three groups were not significant. The clinical pregnancy rate in Frozen D5-ET Group was 62.15%, which was significantly higher than other two groups (46.10%;38.93%;P oups were similar. The ectopic pregnancy rate in Frozen D5-ET Group was 0.41%, which was significantly lower than that in Frozen D3-ET Group (2.99%, P oup (2.29%, P = 0.089). Results from this study show that the transfer of frozen D5 blastocysts can significantly decrease the incidence rate of ectopic pregnancy.

关键词： Fresh Embryo Transfer Blastocyst Transfer Ectopic Pregnancy

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点可迁图的限制边连通性

数学物理学报（A辑） 2005年第6期25卷 863-868页

作者：欧见平五邑大学数理系江门529020

3限制边割是连通图的一个边割,它将此图分离成阶不小于3的连通分支.图G的最小3限制边割所含的边数称为此图的3限制边连通度,记作λ3(G).它以图G的3阶连通点导出子图的余边界的最小基数ξ3(G)为上界.如果λ3(G)=ξ3(G),则称图G是极大3限... 详细信息

3限制边割是连通图的一个边割,它将此图分离成阶不小于3的连通分支.图G的最小3限制边割所含的边数称为此图的3限制边连通度,记作λ3(G).它以图G的3阶连通点导出子图的余边界的最小基数ξ3(G)为上界.如果λ3(G)=ξ3(G),则称图G是极大3限制边连通的.已知在某种程度上,3限制边连通度较大的网络有较好的可靠性.作者在文中证明如果k正则连通点可迁图的围长至少是5,那么它是是极大3限制边连通的.

关键词：点可迁图边连通度限制