Homology-based repair induced by CRISPR-Cas nucleases in mammalian embryo genome editing

作     者：Xiya Zhang Tao Li Jianping Ou Junjiu Huang Puping Liang Xiya Zhang;Tao Li;Jianping Ou;Junjiu Huang;Puping Liang

作者机构：Center for Reproductive Medicinethe Third Affiliated Hospital of Sun Yat-sen UniversitySun Yat-sen UniversityGuangzhou 510630China MOE Key Laboratory of Gene Function and RegulationState Key Laboratory of BiocontrolSchool of Life SciencesSun Yat-sen UniversityGuangzhou 510275China Key Laboratory of Reproductive Medicine of Guangdong Provincethe First Affiliated Hospital and School of Life SciencesSun Yat-sen UniversityGuangzhou 510275China 

出 版 物：《Protein & Cell》 (蛋白质与细胞（英文版）)

年 卷 期：2022年第13卷第5期

页      面：316-335页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：the Key Technologies Research and Development Program(2017YFC1001901) the Guangdong Special Support Program(2019BT02Y276) Guangdong Basic and Applied Basic Research Foundation(2018A030313370 and 2021A1515010759) the National Natural Science Foundation of China(31971365) the Guangzhou Science and Technology Project(201803010020) 

主　　题：homology-based repair(HBR) genome editing disease modeling embryo precision medicine 

摘      要：Recent advances in genome editing,especially CRISPR-Cas nucleases,have revolutionized both laboratory research and clinical ***-Cas nucleases,together with the DNA damage repair pathway in cells,enable both genetic diversification by classical non-homologous end joining(c-NHEJ)and precise genome modification by homology-based repair(HBR).Genome editing in zygotes is a convenient way to edit the germline,paving the way for animal disease model generation,as well as human embryo genome editing therapy for some life-threatening and incurable *** efficiency is highly dependent on the DNA donor that is utilized as a repair ***,we review recent progress in improving CRISPR-Cas nuclease-induced HBR in mammalian embryos by designing a suitable DNA ***,we want to provide a guide for producing animal disease models and correcting genetic mutations through CRISPR-Cas nuclease-induced HBR in mammalian ***,we discuss recent developments in precise genome-modification technology based on the CRISPR-Cas system.