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Animal Models and Experimental Medicine 2023年第2期6卷 111-119页

作者： Yajuan Wang qingrong Zhang Shuchi Zhang jiangtao qi Li Li Department of Anesthesiology Huashan HospitalFudan UniversityShanghaiChina Department of Anesthesiology Tongji HospitalTongji UniversityShanghaiChina

Background:TTC(2,3,5-triphenyltetrazolium chloride)staining is the most commonly used method in identifying and assessing cerebral infarct volumes in the transient middle cerebral artery occlusion model.Given that microglia exhibit different morphologies in different regions after ischemic stroke,we demonstrate the superiority and necessity of using TTC-stained brain tissue to analyze the expression of various proteins or genes in different regions based on microglia character.Methods:We compared brain tissue(left for 10 min on ice)from the improved TTC staining method with penumbra from the traditional sampling method.We identified the feasibility and necessity of the improved staining method using real time(RT)-PCR,Western blot,and immunofluorescence analysis.Results:There was no protein and RNA degradation in the TTC-stained brain tissue group.However,the TREM2 specifically expressed on the microglia showed a significant difference between two groups in the penumbra region.Conclusions:TTC-stained brain tissue can be used for molecular biology experiments without any restrictions.In addition,TTC-stained brain tissue shows greater superiority due to its precise positioning.

关键词： ischemic brain injury microglia transient middle cerebral artery occlusion TTC staining

Simple decision-making model for orchard air-assisted spraying airflow

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International Journal of Agricultural and Biological Engineering 2023年第2期16卷 23-29页

作者： Xiang Wang Yuru Feng Wei Fu jiangtao qi Jianli Song Center for Chemicals Application Technology(CCAT) College of ScienceChina Agricultural UniversityBeijing 100193China College of Mechanical and Electrical Engineering Hainan UniversityHaikou 570100China College of Biological and Agricultural Engineering Jilin UniversityChangchun 130022China

Airflow speed is one of the three factors of air-assisted spraying.Optimizing the matching model between airflow speed and target canopy characteristics is an effective way to improve the orchard precision spraying technology,as airflow can significantly affect droplet deposition and drift loss.A simple model of airflow speed was established in this study.First,air-assisted spraying experiments were carried out on a standard simulation canopy to study the airflow speed depended on canopy width,leaf area index,and porosity rate.Second,determined by Ribbon Method and verified by droplet drift data,the airflow speed through the canopy was between 0.5 m/s and 0.7 m/s.Third,multiple tests were carried out under standard simulation canopy with different characteristics,and the airflow speed model was established ultimately:with a fixed leaf area index(LAI),the relationship between canopy upwind boundary airflow speed and canopy width satisfied the exponential model(y=ae^(bx)),and the coefficients a and b are well related to the density of branches and leaves in the canopy.When LAI=3.456,y=2.036e^(1.5887x),R^(2)=0.994;LAI=1.728,y=1.639e^(1.445x),R^(2)=0.972.Orchard growers can acquire needed airflow speed through this simple model,it is quick and precise and appropriate to most growth periods of a variety of fruit trees,such as apples,pears,and vines.

关键词： airflow speed canopy width porosity rate LAI air-assisted spraying

A combined nanotherapeutic approach targeting farnesoid X receptor,ferroptosis,and fibrosis for nonalcoholic steatohepatitis treatment

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Acta Pharmaceutica Sinica B 2024年第5期14卷 2228-2246页

作者： jiangtao Fu Pingping Zhang Zhiguo Sun Guodong Lu qi Cao Yiting Chen Wenbin Wu Jiabao Zhang Chunlin Zhuang Chunquan Sheng Jiajun Xu Ying Lu Pei Wang The Center for Basic Research and Innovation of Medicine and Pharmacy(MOE) School of PharmacyNaval Medical UniversityShanghai 200433China National Demonstration Center for Experimental Pharmaceutical Education Naval Medical University/Second Military Medical UniversityShanghai 200433China Department of Diving and Hyperbaric Medicine Naval Special Medical CenterNaval Medical UniversityShanghai 200433China

Obeticholic acid(OCA),a farnesoid X receptor(FXR)agonist with favorable effects on fatty and glucose metabolism,has been considered the leading candidate drug for nonalcoholic steatohepatitis(NASH)treatment.However,its limited effectiveness in resolving liver fibrosis and lipotoxicity-induced cell death remains a major drawback.Ferroptosis,a newly recognized form of cell death characterized by uncontrolled lipid peroxidation,is involved in the progression of NASH.Nitric oxide(NO)is a versatile biological molecule that can degrade extracellular matrix.In this study,we developed a PEGylated thiolated hollow mesoporous silica nanoparticles(MSN)loaded with OCA,as well as a ferroptosis inhibitor liproxsatin-1 and a NO donor S-nitrosothiol(ONL@MSN).Biochemical analyses,histology,multiplexed flow cytometry,bulk-tissue RNA sequencing,and fecal 16S ribosomal RNA sequencing were utilized to evaluate the effects of the combined nanoparticle(ONL@MSN)in a mouse NASH model.Compared with the OCA-loaded nanoparticles(O@MSN),ONL@MSN not only protected against hepatic steatosis but also greatly ameliorated fibrosis and ferroptosis.ONL@MSN also displayed enhanced therapeutic actions on the maintenance of intrahepatic macrophages/monocytes homeostasis,inhibition of immune response/lipid peroxidation,and correction of microbiota dysbiosis.These findings present a promising synergistic nanotherapeutic strategy for the treatment of NASH by simultaneously targeting FXR,ferroptosis,and fibrosis.

关键词： Nonalcoholic steatohepatitis Nanotherapy Farnesoid X receptor Ferroptosis Fibrosis Obeticholic acid S-Nitrosothiol Liproxstatin-1

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稀溶液中大幅拉伸高分子单链的弛豫动力学

高等学校化学学报 2024年第4期45卷 134-141页

作者：姚宁王海军李江涛顾芳廖琦河北大学化学与材料科学学院保定071002 河北大学河北省化学生物学重点实验室保定071002 河北大学药物化学与分子诊断教育部重点实验室保定071002 中国科学院北京化学研究所北京100190

针对大幅拉伸的高分子单链在Θ溶剂和良溶剂中的弛豫过程,分别通过解析理论和分子动力学模拟方法研究了弛豫动力学.对比研究发现,相对于研究高分子链在平衡态下的弛豫动力学(利用有关物理量的自关联函数),本文的非平衡态方法可以明确地... 详细信息

针对大幅拉伸的高分子单链在Θ溶剂和良溶剂中的弛豫过程,分别通过解析理论和分子动力学模拟方法研究了弛豫动力学.对比研究发现,相对于研究高分子链在平衡态下的弛豫动力学(利用有关物理量的自关联函数),本文的非平衡态方法可以明确地揭示弛豫时间与简正模式之间标度行为的动态演化.结果表明,相关的物理量在弛豫初期均受到非平衡态的影响,而在较长时间后,高分子链的弛豫动力学则与平衡态下的结果相吻合;在高分子链的最长弛豫时间与链长的标度关系方面,平衡态和非平衡态两种方法的研究结果也一致.研究结果表明,高分子链在非平衡态下的短时和长时弛豫动力学可以更加细致地阐明弛豫全过程,给出更加丰富的结构-特征关系,从而为深入探讨高分子链的非平衡弛豫提供可借鉴的线索.

关键词：高分子单链弛豫动力学简正模式弛豫时间

Operation power consumption and verification tests of a trenching device for orchards in Xinjiang based on discrete element

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International Journal of Agricultural and Biological Engineering 2021年第1期14卷 133-141页

作者： Chen Ma jiangtao qi Za Kan Shaojie Chen Hewei Meng College of Mechanical and Electrical Engineering Shihezi UniversityShihezi 832000XinjiangChina College of Mechanical and Electronic Engineering Xinjiang Vocational UniversityUrumqi 830013China Key Laboratory of Northwest Agricultural Machinery of the Ministry of Agriculture Shihezi 832000XinjiangChina Ruiyida Machinery Equipment Co. Ltd.Shihezi Development ZoneShihezi 832000XinjiangChina

According to the agronomic requirements of orchard planting pattern and fertilization in Xinjiang,the soil particle models and a soil-trenching device interaction model were established by using discrete element simulation software in order to find out the law of influence of structure parameters and working parameters on the operation power consumption of a segmented cutting trenching device;moreover,its cutting process was simulated according to the response surface test design.According to the results of research on the influence of forward speed,cutter disk speed and cutter disk combination on the operation power consumption of the segmented cutting trenching device,the law of their influence on the operation power consumption in descending order is forward speed,cutter disk combination,cutter disk speed.According to the response surface test result,the following influential factor parameter combination has been obtained:forward speed 815 m/h,cutter disk speed 112 r/min,cutter disk combination A(cut angle 150°,bent angle 60°and working width 133 mm),cutter disk combination B(cut angle 180°,bent angle 90°and working width 166 mm),and trenching operation power consumption-optimization results were verified through field tests.According to the verification,the results showed a high accuracy of the simulation,with a relative error mean of 3.18%between the theoretical value and the field test value.This study can provide a reference for the energy-saving design of orchard trenching devices.

关键词： trenching device discrete element orchard power consumption simulation analysis parameter optimization

东海北部滨海湿地铁氧化细菌的分布及其对不同氧气穿透深度的响应

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微生物学报 2024年第6期64卷 1704-1720页

作者：祁文龙宿蕾马忠霖王鹏李江涛同济大学海洋地质国家重点实验室上海200092

【目的】滨海湿地生态系统位于淡水与海水交互地带,含有高浓度Fe^(2+)的地下水渗透到沉积物表层形成的湿地径流和周期性潮汐淹水形成的含氧-缺氧界面有利于铁氧化细菌介导的Fe^(2+)的生物氧化过程发生。然而,目前缺乏对滨海湿地生态系... 详细信息

【目的】滨海湿地生态系统位于淡水与海水交互地带,含有高浓度Fe^(2+)的地下水渗透到沉积物表层形成的湿地径流和周期性潮汐淹水形成的含氧-缺氧界面有利于铁氧化细菌介导的Fe^(2+)的生物氧化过程发生。然而,目前缺乏对滨海湿地生态系统中铁氧化细菌类群的全面评估。【方法】以上海崇明西沙湿地公园及浙江舟山市朱家尖岛东沙沙滩两地共5处滨海湿地沉积物为研究对象,分析沉积物的氧气穿透深度等环境参数,并基于16S rRNA基因扩增子测序技术,全面解析不同滨海湿地生态系统中细菌与铁氧化细菌的群落组成与分布特征。【结果】与崇明西沙湿地相比,朱家尖岛东沙沙滩有更深的氧气穿透深度,达到10 mm以上。非度量多尺度分析(non-metric multidimensional scaling,NMDS)统计结果表明,细菌群落结构主要受到区域位置不同导致的环境条件差异的影响,而铁氧化细菌的群落结构则受到采样的区域位置和沉积物氧气穿透深度的共同影响。崇明西沙湿地和朱家尖岛东沙沙滩的优势细菌为蓝细菌门(Cyanobacteria)、γ-变形菌纲(Gammaproteobacteria)、拟杆菌门(Bacteroidetes)、α-变形菌纲(Alphaproteobacteria)和放线菌门(Actinobacteria);优势铁氧化细菌为嘉利翁氏菌属(Gallionella)、红细菌属(Rhodobacter)、Lepthothrix和Sideroxydans。【结论】通过对崇明西沙湿地和朱家尖岛东沙沙滩沉积物中栖息的铁氧化菌的调查发现,铁氧化细菌的群落组成与湿地沉积物类型导致的氧气穿透深度差异具有密切联系。

关键词：崇明西沙湿地朱家尖岛东沙沙滩滨海湿地氧气穿透深度铁氧化细菌

Design and test of bionic wide-ridge soybean tilling-sowing machine

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International Journal of Agricultural and Biological Engineering 2019年第1期12卷 42-51页

作者： Honglei Jia Mingzhuo Guo Jiale Zhao Dongyan Huang Jian Zhuang jiangtao qi College of Biological and Agricultural Engineering Jilin UniversityChangchun 130022China Key Laboratory of Bionic Engineering Ministry of EducationJilin UniversityChangchun 130022China

The insufficient accumulated temperature of the plow layer during spring tillage in Northeast China severely restricts soybean root growth and whole-plant development.High regional soil viscosity further complicates tilling-sowing.In order to seek a solution to these problems,field comparative tests were conducted to investigate the effects of shallow-loosening(SL)and reshaping ridge(RR)on soil temperature and soybean root growth.Compared with conventional tillage(CT),SL and RR significantly increased the soil temperatures within 0-25 cm(p<0.05)and 0-15 cm(p<0.05),respectively.In particular,higher soil temperature within 15-25 cm was established after SL than after RR(p<0.05).Additionally,SL promoted substantially more vigorous soybean development(seedling height)than RR(p<0.05),which in turn led to a significant outperformance over CT(p<0.05).Further,bionics,reverse engineering,and curve fitting were combined to design a hare claw toe bionic shallow-loosening shovel and a pangolin scale bionic ridging shovel with anti-drag functions.Field verification tests confirmed that these two bionic tillage devices outperformed the conventional tillage device in reducing tractive drag by 13%-19%.Based on the results of these tests,a 2BGD-6(110)bionic wide-ridge soybean tilling-sowing machine was designed,which was capable of shallow-loosening,reshaping ridge and sowing.The new machine significantly reduced the tractive drag,efficiently loosened the soil,increased soil temperature,and accelerated soybean root growth.This study can provide a theoretical and practical reference for soybean production in Northeast China.

关键词： bionics tillage shallow-loosening soybean reshaping ridge soil temperature

Design and experiment of a subsoiling variable rate fertilization machine

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International Journal of Agricultural and Biological Engineering 2020年第4期13卷 118-124页

作者： jiangtao qi Xinliang Tian Yang Li Xuhui Fan Hongfang Yuan Jiale Zhao Honglei Jia Key Laboratory of Bionic Engineering Ministry of EducationJilin UniversityChangchun 130022China College of Biological and Agricultural Engineering Jilin UniversityChangchun 130022China Jilin Province Academy of Agricultural Machinery Changchun 130022China

In order to improve soil fertility and fertilizer utilization,a subsoiling variable rate fertilization machine based on conservation tillage and precision agriculture was designed and tested.The relationship between suspension parameters and penetrating distance was analyzed,and a matching model between fertilizing quantity and penetrating distance was established.The variable rate fertilization control machine was developed based on an Advantech PCM-9363 industrial control mainboard.The machine operates under two patterns:DGPS-based positioning and straight-line path positioning based on a planar coordinate system.This machine can perform on-demand fertilization according to the spatial differences in soil nutrients and the prescription maps pre-set before the operation.Field experiments showed the machine has a subsoiling stability of 92.5%,a soil breaking rate of 61.1%,a maximum positioning relative error of 2.68%and a maximum variable rate fertilization error of 3.89%.The subsoiling performance and variable rate fertilization indices of this machine satisfy the requirements of GB/T24675.2-2009.The tested indices meet the national and industrial standards and satisfy the design requirements.The findings of the research can be used as the structural design of the subsoiling variable rate fertilization machine.

关键词： agricultural machinery conservation tillage precision agriculture subsoiling variable rate fertilization

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基于GRU的仪表着陆系统故障预测方法研究

航空工程进展 2024年第3期15卷 62-70页

作者：张强祁江涛焦浩博黄莉莉中国民用航空飞行学院空中交通管理学院广汉618307 中国民用航空飞行学院广汉分院广汉618307

故障预测技术在保障仪表着陆系统的可靠运行、提高空管效能等方面具有重要应用价值。结合仪表着陆系统运行特征和实际运行维护工作,提出一种基于GRU的仪表着陆系统故障预测方法。以航向信标为研究对象,在分析其监控参数与设备运行状态... 详细信息

故障预测技术在保障仪表着陆系统的可靠运行、提高空管效能等方面具有重要应用价值。结合仪表着陆系统运行特征和实际运行维护工作,提出一种基于GRU的仪表着陆系统故障预测方法。以航向信标为研究对象,在分析其监控参数与设备运行状态之间的关系后,将监控参数作为故障特征参数;根据监控参数时间步长、时变性特征显著的特点,采用GRU预测监控参数的未来变化趋势;根据监控参数的隶属函数计算出参数未来时刻可能发生“故障”的概率,实现对航向信标故障的预测。结果表明:基于GRU的仪表着陆系统预测方法的相对预测精度在95%以上。

关键词：仪表着陆系统故障预测监控参数门控循环单元隶属度函数

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预加权调制密集图卷积网络三维人体姿态估计

计算机科学与探索 2024年第4期18卷 963-977页

作者：马金林崔琦磊马自萍闫琦曹浩杰武江涛北方民族大学计算机科学与工程学院银川750021 图像图形智能信息处理国家民委重点实验室银川750021 北方民族大学数学与信息科学学院银川750021

图卷积网络(GCN)日益成为三维人体姿态估计(3D HPE)的主要研究热点之一,使用GCN对人体关节点之间的关系建模的方法使三维人体姿态估计获得了良好的性能。然而,基于GCN的三维人体姿态估计方法存在过平滑和未区分关节点与相邻关节点重要... 详细信息

图卷积网络(GCN)日益成为三维人体姿态估计(3D HPE)的主要研究热点之一,使用GCN对人体关节点之间的关系建模的方法使三维人体姿态估计获得了良好的性能。然而,基于GCN的三维人体姿态估计方法存在过平滑和未区分关节点与相邻关节点重要性的问题。为解决这些问题,设计了调制密集连接模块(MDC)和预加权图卷积模块,并基于这两个模块提出了预加权调制密集图卷积网络的三维人体姿态估计方法(WMDGCN)。针对过平滑问题,调制密集连接通过超参数α和β更好地实现特征重用(超参数α表示第l层和之前各层总特征的权重比例,超参数β表示之前各层特征到第l层的传播策略),从而有效地提高特征的表达能力。针对未区分关节点与相邻关节点重要性的问题,使用预加权图卷积为当前关节点赋予更高的权重,并对当前关节点及其相邻关节点使用不同的权重矩阵,更有效地捕获人体关节点特征。Human3.6M数据集上的对比实验结果表明,该方法在参数量和性能上均取得了最佳性能,WMDGCN的参数量、MPJPE和P-MPJPE值分别为0.27 MB、37.46 mm和28.85 mm。

关键词：三维人体姿态估计图卷积网络预加权