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Effects of the activities of key enzymes involved in starch biosynthesis on the fine structure of amylopectin in developing rice (Oryza sativa L.) endosperms

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Science china(Life Sciences) 2008年第10期51卷 863-871页

作者： Lü Bing, GUO ZhiGang & LIANG JianSheng College of Bioscience and Biotechnology, Key Laboratory of Crop Genetics and Physiology of jiangsu province, Yangzhou Univer- sity, Yangzhou 225009, china 1. College of Bioscience and Biotechnology Key Laboratory of Crop Genetics and Physiology of Jiangsu Province Yangzhou University Yangzhou 225009 China

The dynamic changes of the activities of enzymes involving in starch biosynthesis, including ADP-glucose pyrophosphorylase (AGPase), soluble starch synthases (SSS), starch branching enzyme (SBE) and starch debranching enzymes (DBE) were studied, and changes of fine structure of amy- lopectin were characterized by isoamylase treatment during rice grain development, using trans anti-waxy gene rice plants. The relationships between the activities of those key enzymes were also analyzed. The amylose synthesis was significantly inhibited in transgenic Wanjing 9522, but the total starch content and final grain weight were less affected as compared with those of non-transgenic Wanjing 9522 rice cultivar. Analyses on the changes of activities of enzymes involving in starch bio- synthesis showed that different enzyme activities were expressed differently during rice endosperm development. Soluble starch synthase is relatively highly expressed in earlier stage of endosperm de- velopment, whilst maximal expression of granule-bound starch synthase (GBSS) occurred in mid-stage of endosperm development. No obvious differences in changes of the activities of AGPase and SBE between two rice cultivars investigated, except the DBEs. Distribution patterns of branches of amy- lopectin changed continually during the development of rice grains and varied between two rice culti- vars. It was suggested that amylopectin synthesis be prior to the synthesis of amylose and different enzymes have different roles in controlling syntheses of branches of amylopectin.

关键词： ADP-glucose pyrophosphorylase amylopectin structure granule-bound starch synthase Oryza sativa soluble starch synthase starch branching enzyme starch debranching enzyme

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Source mechanism of strong aftershocks (M_s≥5.6) of the 2008/05/12 Wenchuan earthquake and the implication for seismotectonics

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Science china Earth Sciences 2009年第6期52卷 739-753页

Dozens of >M5, hundreds of >M4, and much more >M3 aftershocks occurred after the 2008/05/12 Wenchuan earthquake, which were well recorded by permanent and portable seismic stations. After relocated with P arrival, the >M3 aftershocks show two trends of distribution, with most of the aftershocks located along the north-east strike consistent with Longmenshan fault system, yet there is a north-west trend around the epicenter. It seems that substantially more aftershocks occur in regions with crystalline bedrocks. Then we collected waveform data from National Digital Seismograph Network and regional seismograph network of china, and employed 'Cut and Paste' method to obtain focal mechanisms and depths of the big aftershocks (M≥5.6). While most of those aftershocks show thrust mechanism, there are some strike slip earthquakes in the northern-most end of the rupture. Focal mechanisms show that the events located on the southern part of central Beichuan-Yingxiu Fault (BY) are mainly thrust earthquakes, which is consistent with initial mechanism of the main shock rupture. In the north part the aftershocks along the BY are also dominated by thrust slip, which is quite different from the right slip rupture of the main shock. Around Qingchuan-Pingwu Fault, the focal mechanisms are dominated by right-slip rupture with large depths (~18 km). So we suspected that in the north part the main shock might rupture on two faults: Beichuan Fault and Qingchuan-Pingwu Fault. The complex pattern of aftershock mechanisms argues for presence of a complicated fault system in the Longmenshan area.

关键词： focal mechanism seismotectonics Wenchuan earthquake cut and paste.

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Melatonin ameliorates experimental hepatic fibrosis induced by carbon tetrachloride in rats

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World Journal of Gastroenterology 2009年第12期15卷 1452-1458页

作者： Ru-Tao Hong,Department of Geriatrics Medicine,The First Affiliated Hospital of Anhui Medical University,Hefei 230022,Anhui province,china Ru-Tao Hong,Jian-Ming Xu,Qiao Mei,Department of Gastroenterology,The First Affiliated Hospital of Anhui Medical University,Hefei 230022,Anhui province,china The Key Laboratory of Digestive Diseases of Anhui province,Hefei 230022,Anhui province,china Department of Gastroenterology The First Affiliated Hospital of Anhui Medical University Hefei 230022 Anhui Province China. xhkay@***

AIM:To investigate the protective effects of melatonin on carbon tetrachloride(CCl4)-induced hepatic fibrosis in experimental ***:All rats were randomly divided into normal control group,model control group treated with CCl4 for 12 wk,CCl4+NAC group treated with CCl4+NAC(100 mg/kg,i.p.)for 12 wk,CCl4+MEL-1 group treated with CCl4+melatonin(2.5 mg/kg)for 12 wk,CCl4+MEL-2 group treated with CCl4+ melatonin(5.0 mg/kg)for 12 wk,and CCl4+MEL-3 group treated with CCl4+melatonin(10 mg/kg).Rats in the treatment groups were injected subcutaneously with sterile CCl4(3 mL/kg,body weight)in a ratio of 2:3 with olive oil twice a *** in normal control group received hypodermic injection of olive oil at the same dose and frequency as those in treatment *** the end of experiment,rats in each group were anesthetized and *** and eosin(HE)staining and Van Gieson staining were used to examine changes in liver *** activities of alanine aminotransferase(ALT),aspartate aminotransferase(AST)and protein concentration weremeasured with routine laboratory methods using an ***(HYP)content in liver and malondialdehyde(MDA)and glutathione peroxidase(GPx)levels in liver homogenates were assayed by *** hyaluronic acid(HA),laminin(LN),and procollagenⅢN-terminal peptide(PⅢNP)were determined by ***:Pathologic grading showed that the fibrogenesis was much less severe in CCl4+MEL3 group than in model control group(u=2.172,P<0.05),indicating that melatonin(10 mg/kg)can significantly ameliorate CCl4-induced hepatic fibrotic *** serum levels of ALT and AST were markedly lower in CCl4+MEL treatment groups(5,10 mg/kg)than in model control group(ALT:286.23 ±121.91 U/L vs 201.15±101.16 U/L and 178.67 ±103.14 U/L,P=0.028,P=0.007;AST:431.00 ±166.35 U/L vs 321.23±162.48 U/L and 292.42 ±126.23 U/L,P=0.043,P=0.013).Similarly,the serum laminin(LN)and hyaluronic acid(HA)levels and hydroxyproli

关键词： Melatonin Hepatic fibrosis Oxidativestress Hyaluronic acid Laminin Malondialdehyde Glutathione peroxidase

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Average slip rate, earthquake rupturing segmentation and recurrence behavior on the Litang fault zone, western Sichuan province, china

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Science china Earth Sciences 2005年第8期48卷 1183-1196页

作者： XU Xiwei1, WEN Xueze2, YU Guihua1, ZHENG Rongzhang1, LUO Haiyuan2 & ZHENG Bin2 1. Institute of Geology, china Earthquake Administration, Beijing 100029, china 2. Earthquake Administration of Sichuan province, Chengdu 610041, china 1. Institute of Geology China Earthquake Administration 100029 Beijing China 2. Earthquake Administration of Sichuan province 610041 Chengdu China

The Litang fault zone (LFZ) is an active fault zone within the northwestern Sichuan sub-block. Field investigation reveals new evidence for its late Quaternary offset, neoteric earthquake ruptures and surface-rupturing segmentation, from which long-term slip-rates, char-acteristic earthquake magnitudes and recurrence intervals on the fault zone are estimated. This study shows that the LFZ consists of three subordinate faults, and they are the northern Maoyaba fault, Litang fault and Kangga-Dewu fault, respectively. All of them are dominated by left-lateral strike-slip with reverse dip-slip component on different segments. Based on offset landforms and ages of relevant deposits collected from seven sites, it is estimated that the average left-lateral slip-rate on the LFZ reaches 4.0±1.0 mm/a in the past 14 ka BP, and vertical (reverse) dip-slip rate in the range of 0.1―1.8 mm/a. Three subordinate faults are independent earthquake rup-turing segments, on which the maximum moment magnitude of the characteristic earthquakes is estimated to be 7.0―7.3, and their average recurrence interval to be 500―1000 a. The latest earthquake ruptures occurred 119±2 years ago on the northern Maoyaba fault, about 1890 AD on the Litang fault, and in 1948 AD on the Kangga-Dewu fault, and this may indicate a unidirectional migration for surface rupturing earthquakes along the Litang fault zone, related to stress trigger-ing between the segments.

关键词： Litang fault zone, slip rate, earthquake rupturing segmentation, recurrence interval.

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Gene flow from transgenic oilseed rape (Brassica napus L.) to cruciferous weeds under mentor pollen inducement

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Progress in Natural Science:Materials International 2007年第11期17卷 1284-1289页

作者： Zhao Xiangxiang1,2, Luo Yuming1, Lu Weiping2, Qi Cunkou3, Pu Huiming3 and Wang Youping2(1. Biotechnology Key Laboratory of Eco-agriculture in Hongze Lake Region, jiangsu province, Huaiyin Normal College, Huai'an 223001, china 2. jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, china 3. Institute of Industrial Crops, jiangsu Academy of Agricultural Sciences, Nanjing 210014, china) Biotechnology Key Laboratory of Eco-agriculture in Hongze Lake Region Jiangsu Province Huaiyin Normal College Huai'an 223001 China jiangsu Provincial Key Laboratory of Crop Genetics and Physiology Yangzhou University Yangzhou 225009 China Institute of Industrial Crops Jiangsu Academy of Agricultural Sciences Nanjing 210014 China

The alien gene flow between genetically modified glyphosate-resistant rapeseed variety Q3 (Brassica napus L.) and four cruciferous weeds was studied under mentor pollen inducement. The results showed that when Thlaspi arvense L., Capsella bursa-pastoris (L.) Medic, Cardamine hirsuta L. and Rorippa palustris (L.) Besser were pollinated with mentor pollen, the mixed Q3 and the weed, pollen grains aggregated largely and germinated quickly, and the numbers of pollen tubes penetrating into the style and the ovary were greatly increased as compared with corresponding self-pollination groups. Twenty four to forty eight hours after pollination, several pollen tubes were observed to penetrate into the ovule via micropyle in each mentor combination. However, when the mentor progenies were analyzed by PCR, all of them showed negative for the Q3 herbicide-resistant gene. Collectively, these results indicated that crossing between T. arvense, C. bursa-pastoris, C. hirsuta, R. palustris (as female) and Q3 (as male) was highly incompatible and the herbicide-resistant gene could not flow from Q3 to these four weeds.

关键词： herbicide-resistant rapeseed cruciferous weeds mentor pollination aniline blue fluorescence gene flow

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Assessment of time-dependent seismic hazards on segments of active fault, and its problems

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Chinese Science Bulletin 1998年第23期43卷 1937-1950页

作者： WEN Xueze Seismological Bureau of Sichuan province, Chengdu 610041, china Seismological Bureau of Sichuan province Chengdu China

Assessment of time dependent seismic hazards on segments of active fault is one of the important techniques of long term earthquake forecast for specific locations. It is based on the data from quantitatively seismo geologic investigation in active faults and expresses seismic hazards of a fault segment with both the earthquake occurrence probability, which increases as the elapsed time increases, and the magnitude predicted. Through summing up and analyzing main technical steps in research of the recent decade, some defects in the current theory and model of this technique are pointed out. For instance, the probabilistic models for recurrence interval are based solely on the quasi periodic behavior of earthquake recurrence, ignoring behavior’s diversity that possibly exists. Variability of repeated rupture dimensions in various cycles is considered inadequately in fault segmentation research, etc. It is most important for improving the reliability of the result of seismic hazard assessment to reveal actual earthquake recurrence behavior and its generalities, and to update relevant models for evaluating probabilistic earthquake hazard based on the behavior and generalities.

关键词： active fault rupture segmentation time dependent hazard probabilistic model long term forecast.

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The effect of unstable inertia-gravity wave on the occurrence and the development of typhoon and tropical cyclone

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Acta Oceanologica Sinica 1990年第4期9卷 539-546页

作者： Niu Xuexin Meteorological Bureau of Zhejiang province, Hangzhou, china Meteorological Bureau of Zhejiang province Hongzhou China. China

In this paper,the analysis of the occurrence and the development of typhoon and tropical cyclone is made with the unstable theory of wave. The result indicates that the primary wave is the unstable inertia-gravity wave in the process of the occurrence and the deveJopmant of typhoon and tropical cyclone: The existence of the deep moist layer and the heating by moisture condensation can impel and intensify the unstability of the wave and is favourable for the reduction of the wave energy dispersion, therefore, it is good for the formation and the development of typhoon and tropical cyclone, and also can slow down the wave speed. Besides, the condition that the change of the specific volume of the basic state with pressure is less than that of adiabatic state may also lead to the wave unstability, thus may have certain effect on the occurrence and the development of typhoon and tropical cyclone.

关键词： wave The effect of unstable inertia-gravity wave on the occurrence and the development of typhoon and tropical cyclone

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数字经济背景下物联网技术的司法应用研究

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人民司法 2024年第13期 59-64页

作者：江苏省高级人民法院课题组不详

数字经济的蓬勃发展对司法公正与效率提出了更高要求,在数字中国、网络强国、科技强国重大背景下,物联网技术在司法领域不断应用,探索了物联网电子封条、智能称重系统、动态监管系统、智能送达终端等创新场景,充分发挥了物联网的司法功... 详细信息

数字经济的蓬勃发展对司法公正与效率提出了更高要求,在数字中国、网络强国、科技强国重大背景下,物联网技术在司法领域不断应用,探索了物联网电子封条、智能称重系统、动态监管系统、智能送达终端等创新场景,充分发挥了物联网的司法功效。实践中的物联网司法应用,存在应用场景有待拓展、平台安全与数据安全巫函需重视、法律风险防范尚有缺失等问题,需要树立物联网司法应用新理念,发挥物联网感知互联万物的特性,贯彻科技向善理念,加强物联网基础能力建设,拓展司法应用场景,强化物联网司法应用配套保障,有效做实提质增效,服务保障新质生产力高质量发展。

关键词：司法应用司法领域物联网技术科技强国法律风险防范数据安全物联网感知网络强国

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Cyclooxygenase-2 polymorphisms and susceptibility to gastric carcinoma: A meta-analysis

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World Journal of Gastroenterology 2010年第43期16卷 5510-5517页

作者： Jia-Li Liu, Zhen-Ning Wang, Department of Surgical Oncology and General Surgery, the First Affiliated Hospital of china Medical University, Shenyang 110001, Liaoning province, china Yuan Liang, Department of Oncology, the Shengjing Affiliated Hospital of china Medical University, Shenyang 110001, Liaoning province, china Xin Zhou, Department of Obstetrics and Gynecology, the Shengjing Affiliated Hospital of china Medical University, Shenyang 110001, Liaoning province, china Li-Li Xing, Department of Oncology, the Fourth Affiliated Hospital of china Medical University, Shenyang 110032, Liaoning province, china Department of Surgical Oncology and General Surgery the First Affiliated Hospital of China Medical University Shenyang 110001 Liaoning Province China. josieon826@***

AIM: To investigate the association of the cyclooxygenases-2 (COX-2) polymorphisms and susceptibility to gastric cancer (GC) by means of meta-analysis. METHODS: Publications addressing the association between polymorphisms of COX-2 and susceptibility to GC were selected from the MEDLINE, EMBASE and CBMdisc databases. Data was extracted from the studies by 2 independent reviewers. The meta-analyses were performed by RevMan 5.0.23. From these data, odds ratio (OR) with 95% confidence interval (CI) was ***: Ten studies were retrieved reporting a total of 11 COX-2 polymorphisms. Carriers of -765C, -1195A, -1290G, *2430T alleles and *429TT genotype revealed increased risk for GC (OR = 1.71, 95% CI: 1.01-2.90, P = 0.05; OR = 1.58, 95% CI: 1.05-2.38, P = 0.03; OR = 1.55, 95% CI: 1.01-2.39, P = 0.05; OR = 2.62, 95% CI: 1.20-5.73, P = 0.02 and OR = 0.74, 95% CI: 0.59-0.95, P = 0.02, respectively). CONCLUSION: The -765C, -1195A, -1290G, *2430T alleles and *429TT genotype of COX-2 polymorphisms were determined a significant association with susceptibility to GC.

关键词： Cyclooxygenases-2 Gastric cancer Polymorphisms Susceptibility Meta-analysis

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安徽合肥滨湖新区租赁住房项目

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建筑实践 2024年第3期 88-95页

作者： Anhui province Architectural Design and Research Institute Co., Ltd. 安徽省建筑设计研究总院股份有限公司

项目位于合肥市滨湖新区贵州路与遵义路交叉口,以自由寓、未来苑、天空城为设计主题,强调开放性、公共性,打造配套设施齐全、生活便利的生态住所;充分利用屋面空间,营造追寻自然、健康,及对美好生活向往的空中公园。为避免城市界面过于...

项目位于合肥市滨湖新区贵州路与遵义路交叉口,以自由寓、未来苑、天空城为设计主题,强调开放性、公共性,打造配套设施齐全、生活便利的生态住所;充分利用屋面空间,营造追寻自然、健康,及对美好生活向往的空中公园。为避免城市界面过于封闭呆板,采用两个体量相同、相互错动的居住组团,营造开放、活泼的城市界面;组团内住宅楼围合布置,形成安全、稳定的居住氛围;南北组团紧密联系,内外空间相互协调。配套设施位于两个组团底层,并顺应城市人流方向在西北角设置外街服务带,形成两环—带的布局模式,最大程度对外开放。利用北侧组团2层屋面打造空中跑道,满足健康生活的需求。

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