Melatonin ameliorates experimental hepatic fibrosis induced by carbon tetrachloride in rats

作     者：Ru-Tao Hong,Department of Geriatrics Medicine,The First Affiliated Hospital of Anhui Medical University,Hefei 230022,Anhui Province,China Ru-Tao Hong,Jian-Ming Xu,Qiao Mei,Department of Gastroenterology,The First Affiliated Hospital of Anhui Medical University,Hefei 230022,Anhui Province,China The Key Laboratory of Digestive Diseases of Anhui Province,Hefei 230022,Anhui Province,China 

作者机构：Department of Gastroenterology The First Affiliated Hospital of Anhui Medical University Hefei 230022 Anhui Province China. xhkay@*** 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2009年第15卷第12期

页      面：1452-1458页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by The Natural Science Foundation of Anhui Province No.01043904 the Natural Science Research Project of Colleges and Universities of Anhui Province No.KJ2007B146 

主　　题：Melatonin Hepatic fibrosis Oxidativestress Hyaluronic acid Laminin Malondialdehyde Glutathione peroxidase 

摘      要：AIM:To investigate the protective effects of melatonin on carbon tetrachloride(CCl4)-induced hepatic fibrosis in experimental ***:All rats were randomly divided into normal control group,model control group treated with CCl4 for 12 wk,CCl4+NAC group treated with CCl4+NAC(100 mg/kg,i.p.)for 12 wk,CCl4+MEL-1 group treated with CCl4+melatonin(2.5 mg/kg)for 12 wk,CCl4+MEL-2 group treated with CCl4+ melatonin(5.0 mg/kg)for 12 wk,and CCl4+MEL-3 group treated with CCl4+melatonin(10 mg/kg).Rats in the treatment groups were injected subcutaneously with sterile CCl4(3 mL/kg,body weight)in a ratio of 2:3 with olive oil twice a *** in normal control group received hypodermic injection of olive oil at the same dose and frequency as those in treatment *** the end of experiment,rats in each group were anesthetized and *** and eosin(HE)staining and Van Gieson staining were used to examine changes in liver *** activities of alanine aminotransferase(ALT),aspartate aminotransferase(AST)and protein concentration weremeasured with routine laboratory methods using an ***(HYP)content in liver and malondialdehyde(MDA)and glutathione peroxidase(GPx)levels in liver homogenates were assayed by *** hyaluronic acid(HA),laminin(LN),and procollagenⅢN-terminal peptide(PⅢNP)were determined by ***:Pathologic grading showed that the fibrogenesis was much less severe in CCl4+MEL3 group than in model control group(u=2.172,P0.05),indicating that melatonin(10 mg/kg)can significantly ameliorate CCl4-induced hepatic fibrotic *** serum levels of ALT and AST were markedly lower in CCl4+MEL treatment groups(5,10 mg/kg)than in model control group(ALT:286.23 ±121.91 U/L vs 201.15±101.16 U/L and 178.67 ±103.14 U/L,P=0.028,P=0.007;AST:431.00 ±166.35 U/L vs 321.23±162.48 U/L and 292.42 ±126.23 U/L,P=0.043,P=0.013).Similarly,the serum laminin(LN)and hyaluronic acid(HA)levels and hydroxyproli