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Comparative Analysis of Hina Gene Sequences in Wild (Hordeum spontaneum) and Cultivated (H. vulgare) Barleys

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Agricultural Sciences in China 2011年第9期10卷 1313-1322页

作者： LI Wei-tao jiang qian-tao CHEN Guo-yue PU Zhi-en LIU Ya-xi WANG Ji-rui ZHENG You-liang WEI Yu-ming Triticeae Research Institute Sichuan Agricultural University Wenjiang 611130 P.R.China Key Laboratory of Southwestern Crop Germplasm Utilization Ministry of Agriculture/College of Agriculture Sichuan Agricultural University Ya＇an 625014 P.R.China

The Hina gene is one of the two known Hin genes for hardness, and its RNA expression is correlated with grain hardness and dry matter digestibility variation. In this study, only one clone ofHina gene was obtained from one barley accession. A total of 121 Hina gene sequences were isolated from 121 wild barley （Hordeum spontaneum） accessions in Israel, Iran, and Turkey, and then their molecular characteristics were compared with 97 Hina gene sequences from 74 cultivated barley （H. vulgare） lines in Europe and 23 landrace （H. vulgare） with global distribution and other 26 Hina gene sequences from cultivated barleys （H. vulgare） with unknown global distribution. Cis-acting regulatory element （CARE） searching revealed that there were different types of regulatory element for the Hina gene in wild and landrace/cultivated barleys. There were six consistent cis-acting binding sites in wild and landrace/cultivated barleys, whereas 8 to 16 inconsistent TATA-boxes were observed. In addition, three special elements （E2Fb, Spl, and boxS） were only observed in wild barley, while one （AT1-motif） was only found in landrace/cultivated barley. Forty-four deduced amino acid sequences of HINA from wild and landrace/cultivated barleys were obtained by deleting repetitive amino acid sequences, and they were clustered into two groups on the basis of Neighbor-Joining analysis. However, there was no obvious difference in the amino acid sequences of HINA between wild and landrace/cultivated barleys. Comparing to protein secondary structure of wheat PINA, it was indicated that HINA also existed a signal peptide. In addition, HINA was a hydrophilic protein on the basis of the protein properties and composition.

关键词： Hina secondary structure regulatory element grain hardness barley

Characterization of starch morphology, composition, physicochemical properties and gene expressions in oat

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Journal of Integrative Agriculture 2015年第1期14卷 20-28页

作者： ZHENG Ke jiang qian-tao WEI Long ZHANG Xiao-wei MA Jian CHEN Guo-yue WEI Yu-ming Mitchell Fetch Jennifer LU Zhen-xiang ZHENG You-liang Triticeae Research Institute Sichuan Agricultural University Lethbridge Research Centre Agriculture and Agri-Food Canada Cereal Research Centre Agriculture and Agri-Food Canada Key Laboratory of Southwestern Crop Germplasm Utilization Ministry of Agriculture/Sichuan Agricultural University

Starch is the major carbohydrate in oat （Avena sativa L.） and starch formation requires the coordinated actions of several synthesis enzymes. In this study, the granule morphology, composition and physicochemical properties of oat starch, as well as the expressions of starch synthesis genes were investigated during oat endosperm development. Under the scanning electron microscopy （SEM）, we observed that the unique compound granules were developed in oat endosperms at 10 days post anthesis （DPA） and then fragmented into irregular or polygonal simple granules from 12 DPA until seed maturity. The amylose content, branch chain length of degree of polymerization （DP=13-24）, gelatinization temperature and percentage of retrogradation were gradually increased during the endosperm development; whereas the distribution of short chains （DP=6-12） were gradually decreased. The relative expressions of 4 classes of 13 starch synthesis genes characterized in this study indicated that three expression pattern groups were significantly different among gene classes as well as among varied isoforms, in which the first group of starch synthesis genes may play a key role on the initiation of starch synthesis in oat endosperms.

关键词： oat starch granule morphology composition physicochemical property gene expression

Identification and validation of novel loci associated with wheat quality through a genome-wide association study

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Journal of Integrative Agriculture 2022年第11期21卷 3131-3147页

作者： PU Zhi-en YE Xue-ling LI Yang SHI Bing-xin GUO Zhu DAI Shou-fen MA Jian LIU Ze-hou jiang Yun-feng LI Wei jiang qian-tao CHEN Guo-yue WEI Yu-ming ZHENG You-liang College of Agronomy Sichuan Agricultural UniversityChengdu 611130P.R.China Triticeae Research Institute Sichuan Agricultural UniversityWenjiang 611130P.R.China Key Laboratory of Coarse Cereal Processing Ministry of Agriculture and Rural Affairs/College of Food and Biological EngineeringChengdu UniversityChengdu 610106P.R.China Crop Research Institute Sichuan Academy of Agricultural SciencesChengdu 610066P.R.China

Understanding the genetic basis of quality-related traits contributes to the improvement of grain protein concentration(GPC),grain starch concentration(GSC),and wet gluten concentration(WGC)in *** this study,a genome-wide association study(GWAS)based on a mixed linear model(MLM)was performed on 236 wheat accessions,including 160 cultivars and 76 landraces,using a 55K single nucleotide polymorphism(SNP)array in multiple environments.A total of 12 stable QTL/SNPs that control different quality traits in this populations in at least two environments under stripe rust stress were *** these 12,three,seven and two QTLs associated with GPC,GSC and WGC were characterized,respectively,and they were located on chromosomes(chr)1B,1D,2A,2B,2D,3B,3D,5D,and 7D with the phenotypic variation explained(PVE)ranging from 4.2 to 10.7%.Compared with the previously reported QTLs/genes,five QTLs(***-1BL,***-1DS,***-2DL.1,***-2DL.2,and ***-5DL)were potentially *** markers for the SNPs AX-108770574 and AX-108791420 on chr5D associated with wet gluten concentration were successfully *** phenotypes of the cultivars containing the A-allele in AX-108770574and the T-allele in AX-108791420 were extremely significantly(P<0.01)higher than those of the landraces containing the G-or C-allele with respect to the wet gluten concentration in each of the *** KASP markers developed and validated in this study could be utilized in molecular breeding aimed at improving the quality of wheat.

关键词： cultivars landraces grain protein concentration(GPC) grain starch concentration(GSC) wet gluten concentration(WGC) 55K SNP validation

Quantitative Trait Loci Associated with Micronutrient Concentrations in Two Recombinant Inbred Wheat Lines

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Journal of Integrative Agriculture 2014年第11期13卷 2322-2329页

作者： PU Zhi-en YU Ma HE Qiu-yi CHEN Guo-yue WANG Ji-rui LIU Ya-xi jiang qian-tao LI Wei DAI Shou-fen WEI Yu-ming ZHENG You-liang Triticeae Research Institute Sichuan Agricultural University College of Agronomy Sichuan Agricultural University School of Life Science and Engineering Southwest University of Science and Technology

Micronutrient malnutrition affects over three billion people worldwide, especially women and children in developing countries. Increasing the bioavailable concentrations of essential elements in the edible portions of crops is an effective resolution to address this issue. To determine the genetic factors controlling micronutrient concentration in wheat, the quantitative trait locus （QTL） analysis for iron, zinc, copper, manganese, and selenium concentrations in two recombinant inbred line populations was performed. In all, 39 QTLs for ifve micronutrient concentrations were identiifed in this study. Of these, 22 alleles from synthetic wheat SHW-L1 and seven alleles from the progeny line of the synthetic wheat Chuanmai 42 showed an increase in micronutrient concentrations. Five QTLs on chromosomes 2A, 3D, 4D, and 5B found in both the populations showed signiifcant phenotypic variation for 2-3 micronutrient concentrations. Our results might help understand the genetic control of micronutrient concentration and allow the utilization of genetic resources of synthetic hexaploid wheat for improving micronutrient efifciency of cultivated wheat by using molecular marker-assisted selection.

关键词： micronutrient concentration synthetic hexaploid wheat QTL

Effect of high-molecular-weight glutenin subunit Dy10 on wheat dough properties and end-use quality

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Journal of Integrative Agriculture 2023年第6期22卷 1609-1617页

作者： WANG Yan GUO Zhen-ru CHEN Qing LI Yang ZHAO Kan WAN Yong-fang Malcolm JHAWKESFORD jiang Yun-feng KONG Li PU Zhi-en DENG Mei jiang qian-tao LAN Xiu-jin WANG Ji-rui CHEN Guo-yue MA Jian ZHENG You-liang WEI Yu-ming QI Peng-fei State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Chengdu 611130P.R.China Triticeae Research Institute Sichuan Agricultural UniversityChengdu 611130P.R.China Rothamsted Research Harpenden AL52JQUK

High-molecular-weight glutenin subunits(HMW-GSs) are the most critical grain storage proteins that determine the unique processing qualities of wheat. Although it is a part of the superior HMW-GS pair(Dx5+Dy10), the contribution of the Dy10 subunit to wheat processing quality remains unclear. In this study, we elucidated the effect of Dy10 on wheat processing quality by generating and analyzing a deletion mutant(with the Dy10-null allele), and by elucidating the changes to wheat flour following the incorporation of purified Dy10. The Dy10-null allele was transcribed normally,but the Dy10 subunit was lacking. These findings implied that the Dy10-null allele reduced the glutenin:gliadin ratio and negatively affected dough strength(i.e., Zeleny sedimentation value, gluten index, and dough development and stability times) and the bread-making quality;however, it positively affected the biscuit-making quality. The incorporation of various amounts of purified Dy10 into wheat flour had a detrimental effect on biscuit-making quality. The results of this study demonstrate that the Dy10 subunit is essential for maintaining wheat dough strength. Furthermore, the Dy10-null allele may be exploited by soft wheat breeding programs.

关键词： HMW-GS nonsense mutation Dy10-null allele end-use quality

Genetic dissection of wheat uppermost-internode diameter and its association with agronomic traits in five recombinant inbred line populations at various field environments

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Journal of Integrative Agriculture 2021年第11期20卷 2849-2861页

作者： LIU Hang TANG Hua-ping LUO Wei MU Yang jiang qian-tao LIU Ya-xi CHEN Guo-yue WANG Ji-rui ZHENG Zhi QI Peng-fei jiang Yun-feng CUI Fa SONG Yin-ming YAN Gui-jun WEI Yu-ming LAN Xiu-jin ZHENG You-liang MA Jian State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Ministry of Science and Technology/Triticeae Research InstituteSichuan Agricultural UniversityChengdu 611130P.R.China Agriculture and Food CSIROSt LuciaBrisbane 4217Australia College of Agriculture Ludong UniversityYantai 264000P.R.China College of Agronomy and Biotechnology China Agricultural UniversityBeijing 100093P.R.China School of Plant Biology The University of Western AustraliaPerth WA 6009Australia

Uppermost-internode diameter(UID)is a key morphological trait associated with spike development and yield potential in *** understanding of its genetic basis remains largely ***,quantitative trait loci(QTLs)for UID with high-density genetic maps were identified in five wheat recombinant inbred line(RIL)*** total,25 QTLs for UID were detected in five RIL populations,and they were located on chromosomes 1A,1D(3 QTL),2B(2),2D(3),3B,3D,4A,4B(3),4D,5A(5),5B(2),6B,and *** them,five major and stable QTLs(***-2CN-1D.1,***-2SY-1D,***-QZ-2D,***-SC-3D,and ***-AS-4 B)were identified from each of the five RIL populations in multiple ***-2CN-1D.1,***-2SY-1D and ***-SC-3D are novel *** Allele Specific PCR(KASP)markers tightly linked to them were further investigated for developing near-isogenic lines(NILs)carrying the major ***,candidate genes at these intervals harboring major and stable QTLs were predicted,and they were associated with plant development and water transportation in most *** of physical locations of the identified QTL on the‘Chinese Spring’reference genome showed that several QTLs including two major ones,***-2CN-1D.1 and ***-2 SY-1 D,are likely allelic confirming their validity and *** significant relationships detected between UID and other agronomic traits and a proper UID were ***,our results dissected the underlying genetic basis for UID in wheat and laid a foundation for further fine mapping and map-based cloning of these QTLs.

关键词： uppermost-internode diameter wheat yield potential genetic basis quantitative trait loci

Effect of lamellarization on the microstructure and mechanical properties of marine 10Ni5CrMoV steel

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 402-413页

作者： tao Zou Yanwu Dong Zhouhua jiang Shuyang Du Yushuo Li School of Metallurgy Northeastern UniversityShenyang 110819China Key Laboratory of Rolling and Automation Northeastern UniversityShenyang 110819China Key Laboratory for Ecological Metallurgy of Multimetallic Ores(Ministry of Education) Northeastern UniversityShenyang 110819China

Multistage heat treatment involving quenching(Q),lamellarizing(L),and tempering(T)is applied to marine 10Ni5CrMoV *** microstructure and mechanical properties were studied by multiscale characterizations,and the kinetics of reverse austenite transformation,strain hardening behavior,and toughening mechanism were further *** lamellarized specimens possess low yield strength but high toughness,especially cryogenic *** leads to the development of film-like reversed austenite at the martensite block and lath boundaries,refining the martensite structure and lowering the equivalent grain *** analysis of austenite reversion based on the JMAK model shows that the isothermal transformation is dominated by the growth of reversed austenite,and the maximum transformation of reversed austenite is reached at the peak temperature(750℃).The strain hardening behavior based on the modified Crussard-Jaoul analysis indicates that the reversed austenite obtained from lamellarization reduces the proportion of martensite,significantly hindering crack propagation via martensitic transformation during the *** a consequence,the QLT specimens exhibit high machinability and low yield *** with the QT specimen,the ductile-brittle transition temperature of the QLT specimens decreases from-116 to-130℃due to the low equivalent grain size and reversed austenite,which increases the cleavage force required for crack propagation and absorbs the energy of external load,*** work provides an idea to improve the cryogenic toughness of marine 10Ni5CrMoV steel and lays a theoretical foundation for its industrial application and comprehensive performance improvement.

关键词： 10Ni5CrMoV steel lamellarizing reversed austenite cryogenic toughness ductile-brittle transition temperature

A major and stable QTL for wheat spikelet number per spike validated in different genetic backgrounds

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Journal of Integrative Agriculture 2022年第6期21卷 1551-1562页

作者： DING Pu-yang MO Zi-qiang TANG Hua-ping MU Yang DENG Mei jiang qian-tao LIU Ya-xi CHEN Guang-deng CHEN Guo-yue WANG Ji-rui LI Wei QI Peng-fei jiang Yun-feng KANG Hou-yang YAN Gui-jun WEI Yu-ming ZHENG You-liang LAN Xiu-jin MA Jian State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Ministry of Science and Technology/Triticeae Research InstituteSichuan Agricultural UniversityChengdu 611130P.R.China College of Resources Sichuan Agricultural UniversityChengdu 611130P.R.China College of Agronomy Sichuan Agricultural UniversityChengdu 611130P.R.China UWA School of Agriculture and Environment and The UWA Institute of Agriculture Faculty of ScienceUniversity of Western AustraliaCrawley 6009Australia

The spikelet number per spike(SNS)contributes greatly to grain yield in *** various genes that control wheat SNS is vital for yield *** study used a recombinant inbred line population genotyped by the Wheat55K single-nucleotide polymorphism array to identify two major and stably expressed quantitative trait loci(QTLs)for *** of them(***-2SY-2D.1)was reported previously,while the other(***-2SY-7A)was newly detected and further analyzed in this ***-2SY-7A had a high LOD value ranging from 4.46 to 16.00 and explained 10.21-40.78%of the phenotypic ***-2SY-7A was flanked by the markers AX-110518554 and AX-110094527 in a 4.75-cM interval on chromosome arm *** contributions and interactions of both major QTLs were further analyzed and *** effect of ***-2SY-7A was successfully validated by developing a tightly linked kompetitive allele specific PCR marker in an F_(2:3) population and a panel of 101 high-generation breeding wheat ***,several genes including the previously reported WHEAT ORTHOLOG OF APO1(WAPO1),an ortholog of the rice gene ABERRANT PANICLE ORGANIZATION 1(APO1)related to SNS,were predicted in the interval of *** summary,these results revealed the genetic basis of the multi-spikelet genotype of wheat line 20828 and will facilitate subsequent fine mapping and breeding utilization of the major QTLs.

关键词： yield potential QTL detection QTL validation predicted genes tightly linked KASP marker

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型焦煤沥青粘结性能的强化

中南大学学报（自然科学版） 2016年第12期47卷 3971-3976页

作者：钟强杨永斌李骞姜涛戈捷中南大学资源加工与生物工程学院湖南长沙410083

煤沥青是焦粉成型最有效的粘结剂,为了提高型焦强度,采用配加添加剂和细化沥青来强化煤沥青粘结性能,同时对强化机理进行分析。研究结果表明:助剂BJ可显著强化煤沥青的粘结性能,使型焦湿块和炭化块强度均明显提高;添加BJ后的煤沥青由固... 详细信息

煤沥青是焦粉成型最有效的粘结剂,为了提高型焦强度,采用配加添加剂和细化沥青来强化煤沥青粘结性能,同时对强化机理进行分析。研究结果表明:助剂BJ可显著强化煤沥青的粘结性能,使型焦湿块和炭化块强度均明显提高;添加BJ后的煤沥青由固体颗粒态转变为粘稠的胶体态,粘结方式由以固相粘结为主变为以液相粘结为主;对煤沥青进行细磨处理可降低煤沥青热挥发产生的大孔洞数量;与煤沥青共混共磨的超细磨焦粉可填充煤沥青热处理产生的孔隙,与煤沥青共炭化而强化煤沥青的炭化骨架;当煤沥青粒度细磨至粒度小于0.15 mm时,配加质量分数为2.0%的BJ和12%平均粒度为8.6μm的焦粉可显著强化煤沥青粘结性能,大幅度地提高型焦强度。

关键词：型焦煤沥青强化超细焦粉粘结性能