Comparative Analysis of Hina Gene Sequences in Wild (Hordeum spontaneum) and Cultivated (H. vulgare) Barleys

作     者：LI Wei-tao JIANG Qian-tao CHEN Guo-yue PU Zhi-en LIU Ya-xi WANG Ji-rui ZHENG You-liang WEI Yu-ming 

作者机构：Triticeae Research Institute Sichuan Agricultural University Wenjiang 611130 P.R.China Key Laboratory of Southwestern Crop Germplasm Utilization Ministry of Agriculture/College of Agriculture Sichuan Agricultural University Ya＇an 625014 P.R.China 

出 版 物：《Agricultural Sciences in China》 (中国农业科学（英文版）)

年 卷 期：2011年第10卷第9期

页      面：1313-1322页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：supported by the National Basic Research Program of China (2010CB134400) 

主　　题：Hina secondary structure regulatory element grain hardness barley 

摘      要：The Hina gene is one of the two known Hin genes for hardness, and its RNA expression is correlated with grain hardness and dry matter digestibility variation. In this study, only one clone ofHina gene was obtained from one barley accession. A total of 121 Hina gene sequences were isolated from 121 wild barley （Hordeum spontaneum） accessions in Israel, Iran, and Turkey, and then their molecular characteristics were compared with 97 Hina gene sequences from 74 cultivated barley （H. vulgare） lines in Europe and 23 landrace （H. vulgare） with global distribution and other 26 Hina gene sequences from cultivated barleys （H. vulgare） with unknown global distribution. Cis-acting regulatory element （CARE） searching revealed that there were different types of regulatory element for the Hina gene in wild and landrace/cultivated barleys. There were six consistent cis-acting binding sites in wild and landrace/cultivated barleys, whereas 8 to 16 inconsistent TATA-boxes were observed. In addition, three special elements （E2Fb, Spl, and boxS） were only observed in wild barley, while one （AT1-motif） was only found in landrace/cultivated barley. Forty-four deduced amino acid sequences of HINA from wild and landrace/cultivated barleys were obtained by deleting repetitive amino acid sequences, and they were clustered into two groups on the basis of Neighbor-Joining analysis. However, there was no obvious difference in the amino acid sequences of HINA between wild and landrace/cultivated barleys. Comparing to protein secondary structure of wheat PINA, it was indicated that HINA also existed a signal peptide. In addition, HINA was a hydrophilic protein on the basis of the protein properties and composition.