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Asian Herpetological Research 2014年第1期5卷 38-48页

作者： Mi ZHAO Yan SHI jian ZHAO Xinping zhu Kunci CHEN Debo PAN Chengqing WEI Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation Ministry of AgriculturePearl River Fishery Research Institute Chinese Academic of Fishery Science

Matrix metallopeptidase 3 is a zinc-containing proteinase that participates in tissue remodeling and immune responses. In this study, a cDNA encoding matrix metallopeptidase 3 was isolated and characterized from the Asian yellow pond turtle Mauremys mutica（designated as MaMMP3）. The MaMMP3 cDNA is 1805 bp and consists of a 5＇-untranslated region（UTR） of 56 bp, a 3＇-UTR of 243 bp, and an open reading frame（ORF） of 1506 bp encoding 481 amino acids. Homology analysis of MaMMP3 revealed that the MaMMP3 shared 25%–63% similarity to other known MMP3 sequences. The genomic sequence covers 6007 bp. Comparative analysis of the cDNA sequence revealed that the Asian yellow pond turtle MMP3 has eight exons and seven introns. The phylogenetic tree showed that the MaMMP3 is closely related to Gallus gallus MMP3 and Taeniopygia guttata MMP3. The mRNA expression of the MaMMP3 in normal group without any bacterial challenge could be detected in all studied tissues including kidney, heart, live and spleen, with the highest level in the spleen. The results of immune challenge showed that the expression level of MaMMP3 was up-regulated in the spleen and liver. These results provided an important information for studying the roles of Asian yellow pond turtle MMP3 in immunity further.

关键词： Asian yellow pond turtle Mauremys mutica Serratia marcescens Matrix metallopeptidase 3 Immune responses

基于HRP的口腔高值耗材全生命周期管理体系的探索

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现代医院管理 2024年第3期22卷 109-112页

作者：陈凯李玉芳宋健柴茂洲钟怡高波四川大学华西口腔医院成都市615000 陕西中医药大学附属医院陕西省咸阳市712000

目的进一步完善口腔专科医院高值耗材的精细化管理,建立符合医院高质量发展要求的耗材管理体系。方法构建口腔高值医用耗材全生命周期管理体系。以基于医院资源规划(HRP)的高值耗材全流程闭环追溯管理系统上线前、后的耗材管理耗时、库... 详细信息

目的进一步完善口腔专科医院高值耗材的精细化管理,建立符合医院高质量发展要求的耗材管理体系。方法构建口腔高值医用耗材全生命周期管理体系。以基于医院资源规划(HRP)的高值耗材全流程闭环追溯管理系统上线前、后的耗材管理耗时、库存金额、医护满意度、不良事件发生率等为观察指标,验证该管理体系的可行性和有效性。结果研究组在耗材管理时间、不良事件发生率、库存金额低于对照组,医护满意度高于对照组(P<0.05)。结论全生命周期管理及溯源体系可有效缩短管理时间、提高医护满意度、降低不良事件发生率和库存金额。

关键词：口腔高值医用耗材流程管理信息化管理全生命周期溯源管理

Liquid Chromatographic Assay of Peptides Activity with Inhibiting Angiotensin Converting Enzyme

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Chemical Research in Chinese Universities 2010年第5期26卷 712-716页

作者： LIU Jing-bo YU Zhi-peng ZHAO Wen-zhu LU Jing CHEN Feng LIN Song-yi Laboratory of Nutrition and Functional Food Jilin University Changehun 130062 P. R. China Department of Food Science and Human Nutrition Clemson University Clemson SC 29634 USA

A rapid, simple and interference-free method was developed to evaluate the inhibitory activity of hydrolyzed peptides from egg white protein against the angiotensin-converting enzyme. The total reaction volume was 60 μL, saving the cost. The assay was based on a HPLC separation and quantification of the synthetic substrate hippuryl-L-histidyl-L-leucine and its hydrolyzed product-hippuric acid; the separation was performed on a C18 column eluted by a mobile phase of acetonitrile/water（0.5% TFA） at a volume ratio of 25：75. At a signal to noise ratio（S/N） of 10, the detective limit of the quantitation of hippuric acid was （0.4600±0.0097） μmol/L. The standard curve shows a linear response with a slope of 49488 and a correlation coefficient of 0.9995. The assay was adequate for the study of ACE inhibition by Captopril and peptides derived from food protein, and showed a very good correlation with the previous methods.

关键词： Angiotensin converting enzyme HPLC Egg white protein Peptide

冬小麦-夏玉米Ⅱ夏花生周年种植的农田温室气体排放及碳足迹评价

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中国油料作物学报 2024年第3期46卷 664-675页

作者：贾文玘伊淼张佳蕾杨莎孟静静张正郭峰王建国万书波山东省农业科学院山东济南250100 青岛农业大学农学院山东青岛266109 汶上县第一中学山东济宁272500

为探究冬小麦-夏玉米Ⅱ夏花生周年种植模式对农田温室气体排放及碳足迹的影响,设置冬小麦-夏玉米、冬小麦-夏花生、冬小麦-夏玉米Ⅱ夏花生3种轮作种植方式;其中夏玉米Ⅱ夏花生间作设置3∶4,3∶6和6∶8三种行比。通过大田试验研究了周年... 详细信息

为探究冬小麦-夏玉米Ⅱ夏花生周年种植模式对农田温室气体排放及碳足迹的影响,设置冬小麦-夏玉米、冬小麦-夏花生、冬小麦-夏玉米Ⅱ夏花生3种轮作种植方式;其中夏玉米Ⅱ夏花生间作设置3∶4,3∶6和6∶8三种行比。通过大田试验研究了周年农田温室气体排放和碳足迹特征。结果表明,夏玉米Ⅱ夏花生种植模式能够减少农田温室气体的排放,与玉米单作相比,土壤CO_(2)和N_(2)O的平均排放通量降幅分别为10.24%~18.75%和10.78%~23.93%、排放总量降幅分别为8.30%~19.12%和14.09%~26.81%。间作减少了后茬冬小麦(轮作模式下)的温室气体排放,间作处理的后茬冬小麦较玉米单作处理的后茬冬小麦土壤CO_(2)排放通量减少3.79%、排放总量减少3.84%;土壤N_(2)O排放通量减少16.80%、排放总量减少17.66%;土壤CH_(4)排放总量呈现“汇”现象。此外,单作玉米生产过程中碳足迹主要来源是氮肥,占总排放的49.13%;单作花生生产过程中碳足迹主要来源是氮肥和地膜,分别占总排放的23.77%和26.06%;间作模式下碳足迹主要来源是氮肥、柴油和地膜,分别占总排放的31.50%、16.74%、17.92%。间作模式增加了后茬小麦碳排放效率、降低了全球增温潜势和温室气体排放强度。综上,冬小麦-夏玉米Ⅱ夏花生(间作玉米花生行比3:6)周年种植模式能够减少农田碳排放与作物生产过程中的碳足迹,具有良好的生态效益。

关键词：玉米-花生间作农田温室气体排放碳足迹全球增温潜势

维普期刊数据库博看期刊

Research on Two-Step Hydro-Bulge Forming of Ellipsoidal Shell with Larger Axis Length Ratio

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Journal of Harbin Institute of Technology(New Series) 2013年第3期20卷 93-98页

作者： Shi-jian Yuan Lan Hu zhu-Bin He Bu-Gang Teng Zhong-Ren Wang National Key Laboratory of Precision Hot Processing of Metals Harbin Institute of Technology Shanghai Aerospace Equipments Manufacturer

The two-step hydro-bulge forming technique was proposed to manufacture the ellipsoidal shell with the length ratio of the long axis to the short axis larger than 1.4. A central tube was introduced into the first step of the hydro-bulge forming process to constrain the over growth of the short axis during bulging,and then the central tube was replaced with two polar plates in the second step of the hydro-bulge forming process to manufacture an integral ellipsoidal shell. It is shown that the central tube restricts the growth of the short axis and simultaneously reduces the shrunk tendency of the long axis. The wrinkling occurs due to the latitudinal compressive stress at the equator at the early stage of hydro-bulge forming. However,with the increase of internal pressure,the compressive stress areas gradually decrease and finally the tensile latitudinal stress occupies approximately the whole shell,thus the wrinkles are eliminated. A sound ellipsoidal shell with the axis length ratio of 1.8 is obtained after two-step hydro bulging.

关键词： hydro-bulge forming ellipsoidal shell latitudinal stress wrinkling axis length ratio

Lu-Eu共掺杂Ga_(2)O_(3)的光电性质的第一性原理计算

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原子与分子物理学报 2024年第3期41卷 144-151页

作者：邹梦真肖清泉姚云美付莎莎叶建峰唐华谢泉贵州大学大数据与信息工程学院新型光电子材料与技术研究所贵阳550025

宽禁带半导体β-Ga_(2)O_(3)因为具有优良的物理化学性能而成为研究热点.本文基于DFT(Density Functional Theory)的第一性原理方法,先采用PBE(Perdew-Burke-Ernzerhof)中的GGA(Generalized Gradient Approximation)和GGA+U(Generalized Gradient Approximation-Hubbard U)的方法计算了本征β-Ga_(2)O_(3),Lu掺杂浓度为12.5%的β-Ga_(2)O_(3)及Lu-Eu共掺杂浓度为25%的β-Ga_(2)O_(3)结构的晶格常数、能带结构和体系总能量.发现采用GGA+U的方法计算的带隙值更接近实验值,于是采用GGA+U的方法计算了本征β-Ga_(2)O_(3),Lu掺杂的β-Ga_(2)O_(3)以及Lu-Eu共掺杂的β-Ga_(2)O_(3)结构的能态总密度、介电函数、吸收谱以及反射率等.由计算结果得知β-Ga_(2)O_(3)的带隙为4.24 eV,Lu掺杂浓度为12.5%的β-Ga_(2)O_(3)的带隙为2.23 eV,Lu-Eu共掺杂浓度为25%的β-Ga_(2)O_(3)的带隙为0.9 eV,均为直接带隙半导体,掺杂并未改变β-Ga_(2)O_(3)的带隙方式.光学性质计算结果表明在低能区掺杂浓度为12.5%的Lu和Lu-Eu共掺杂浓度为25%的β-Ga_(2)O_(3)的吸收系数和反射率均强于本征β-Ga_(2)O_(3),Lu-Eu掺杂β-Ga_(2)O_(3)的吸收系数和反射率又略强于Lu掺杂β-Ga_(2)O_(3),表明Lu-Eu掺杂β-Ga_(2)O_(3)的材料有望应用于制备红外光电子器件.

关键词：第一性原理 Lu-Eu共掺β-Ga_(2)O_(3) 电子结构光学性质

Targeting autophagy using small-molecule compounds to improve potential therapy of Parkinson’s disease

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Acta Pharmaceutica Sinica B 2021年第10期11卷 3015-3034页

作者： Kai Zhang Shiou zhu Jiamei Li Tingting jiang Lu Feng Junping Pei Guan Wang Liang Ouyang bo Liu State Key Laboratory of Biotherapy and Cancer Center West China HospitalCollaborative Innovation Center of BiotherapySichuan UniversityChengdu 610041China Innovation Center of Nursing Research Nursing Key Laboratory of Sichuan ProvinceWest China HospitalSichuan UniversityChengdu 610041China

Parkinson’s disease(PD),known as one of the most universal neurodegenerative diseases,is a serious threat to the health of the *** current treatment has been demonstrated to relieve symptoms,and the discovery of new small-molecule compounds has been regarded as a promising *** note,the homeostasis of the autolysosome pathway(ALP)is closely associated with PD,and impaired autophagy may cause the death of neurons and thereby accelerating the progress of ***,pharmacological targeting autophagy with small-molecule compounds has been drawn a rising attention so *** this review,we focus on summarizing several autophagy-associated targets,such as AMPK,m TORC1,ULK1,IMPase,LRRK2,beclin-1,TFEB,GCase,ERRα,C-Abelson,and as well as their relevant small-molecule compounds in PD models,which will shed light on a clue on exploiting more potential targeted small-molecule drugs tracking PD treatment in the near future.

关键词： Parkinson’s disease(PD) Autophagy Target Small-molecule compound PD therapy

天智颗粒激活PI3K-AKT信号保护Aβ_(25-35)诱导的PC12细胞凋亡作用研究

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浙江中医杂志 2024年第9期59卷 757-760页

作者：陈博程建安唐镔镔刘平郭忠伟浙江省立同德医院浙江杭州310012 西南医科大学附属中医医院四川泸州646099

目的:研究天智颗粒对Aβ_(25-35)诱导的大鼠嗜铬细胞瘤PC12细胞凋亡的作用及其机制。方法:用不同浓度天智颗粒或天智颗粒+LY294002干预Aβ_(25-35)处理的PC12细胞,通过MTT检测细胞活力,流式细胞仪检测细胞凋亡和氧应激,酶标仪检测Caspas... 详细信息

目的:研究天智颗粒对Aβ_(25-35)诱导的大鼠嗜铬细胞瘤PC12细胞凋亡的作用及其机制。方法:用不同浓度天智颗粒或天智颗粒+LY294002干预Aβ_(25-35)处理的PC12细胞,通过MTT检测细胞活力,流式细胞仪检测细胞凋亡和氧应激,酶标仪检测Caspase 3活性和丙二醛(MDA)含量,免疫印迹法检测Bcl2/Bax、p-AKT/AKT蛋白水平。结果:天智颗粒剂量依赖地改善Aβ_(25-35)诱导的PC12细胞活力下降,抑制其凋亡,降低Caspase 3活性,上调Bcl2/Bax表达,减少氧应激和MDA的生成,同时促进PI3K/AKT信号的活化。而PI3K抑制剂LY294002干预显著地逆转天智颗粒的这些有益作用。结论:天智颗粒能有效地保护Aβ_(25-35)诱导的PC12细胞凋亡,其机制可能与激活PI3K/AKT信号相关。

关键词：天智颗粒阿尔茨海默病 PC12细胞凋亡 PI3K-AKT信号

Risk Prevention and Control for Agricultural Non-Point Source Pollution Based on the Process of Pressure-Transformation-Absorption in Chongqing, China

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Chinese Geographical Science 2021年第4期31卷 735-750页

作者： zhu Kangwen CHEN Yucheng ZHANG Sheng YANG Zhimin HUANG Lei LEI bo XIONG Hailing WU Sheng LI Xixi College of Resources and Environment Southwest UniversityChongqing 400716China Chongqing Engineering Research Center of Rural Cleaning Chongqing 400716China Chongqing Academy of Ecology and Environmental Sciences Chongqing 401147China College of Computer&Information Science Southwest UniversityChongqing 400716China Chongqing Chemical Industry Vocational College^Chongqing 401220 China

According to China’s second national survey of pollution sources, the contribution of agricultural non-point sources(ANS) to water pollution is still high. Risk prevention and control are the main means to control costs and improve the efficiency of ANS, but most studies directly take pollution load as the risk standard, leading to a considerable misjudgment of the actual pollution risk. To objectively reflect the risk of agricultural non-point source pollution(ANSP) in Chongqing, China, we investigated the influences of initial source input, intermediate transformation, and terminal absorption of pollutants via literature research and the Delphi method and built a PTA(pressure kinetic energy, transformation kinetic energy, and absorption kinetic energy) model that covers 12 factors, with the support of geographical information system(GIS) technology. The terrain factor calculation results and the calculation results of other factors were optimized by Python tools to reduce human error and workload. Via centroid migration analysis and Kernel density analysis, the risk level, spatial aggregation degree, and key prevention and control regions could be accurately determined. There was a positive correlation between the water quality of the rivers in Chongqing and the risk assessment results of different periods, indirectly reflecting the reliability of the assessment results by the proposed model. There was an obvious tendency for the low-risk regions transforming into high-risk regions. The proportion of high-risk regions and extremely high-risk regions increased from 17.82% and 16.63%in 2000 to 18.10% and 16.76% in 2015, respectively. And the risk level in the main urban areas was significantly higher than that in the southeastern and northeastern areas of Chongqing. The centroids of all grades of risky areas presented a successive distribution from west to east, and the centroids of high-risk and extremely high-risk regions shifted eastward. From 2000 to 2015, the c

关键词： geographic information system(GIS) agricultural non-point source pollution(ANSP) risk assessment Kernel density Chongqing China

Expression of histone methyltransferase G9a and clinical significance in extrahepatic cholangiocarcinoma

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The Chinese-German Journal of Clinical Oncology 2008年第1期7卷 10-13页

作者： Yongjun Chen jian Luo bo Chen jianming Wang Shengquan Zou Department of General Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

Objective： To investigate the mRNA and protein expression of histone methyltransferase G9a and its clinical significance in extrahepatic cholangiocarcinoma. Methods： Using RT-PCR and Western Blotting to detect the expression of G9a at the level of mRNA and protein in 48 tumor samples and 39 control tissues. Results： The mRNA and protein expression of Gga in extrahepatic cholangiocarcinoma was higher than control statistically （P 〈 0.05） and positively correlated with lymph metastasis （P 〈 0.05） and TNM stage （P 〈 0.01）. Conclusion： The significant difference of Gga expression between tumors and control implicated that the important role of histone methylation disruption resulted from increased G9a expression in extrahepatic cholangiocarcinoma.

关键词： extrahepatic cholangiocarcinoma histone methyltransferase G9a