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Relationship between the expression of IP-10 and IP-10 mRNA in peripheral blood and HBV DNA level in patients with cirrhosis

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Hepatobiliary & Pancreatic Diseases International 2010年第3期9卷 280-286页

作者： Wang, jian Wang, ping-ping xiang, Gui-Ju Hu, Xiao-Bin Anhui Univ Sci & Technol Coll Med Dept Aetiol & Immunol Huainan 232001 Peoples R China Second Miners Hosp Huainan Xinhua Hosp Huainan Dept Infect Dis Huainan 232052 Peoples R China

BACKGROUND: Post-hepatitic cirrhosis is regarded as common and severe form of liver damage. Interferon gamma-inducible protein 10 (IP-10), a member of the non-ELR (glutamic-leucine-arginine) motif CXC chemokine family, has recently been shown to recruit and activate specific subsets of leukocytes to sites of inflammation or an immune response during the development of hepatic cirrhosis. However, the effects of IP-10 and IP-10 mRNA on inflammatory infiltration at local sites and in the peripheral blood of patients with post-hepatitic cirrhosis as well as their relationship with viral load are still poorly defined. This study aimed to detect the relationship between the expression of IP-10 in serum, IP-10 mRNA in peripheral blood mononuclear cells (PBMCs), and the levels of HBV DNA in the serum of patients, and to explore their role in the pathogenesis of cirrhosis. METHODS: Typical patients with cirrhosis after HBV infection were selected, and their serum IP-10 concentrations were evaluated with ELISA, the content of IP-10 mRNA in PBMCs was measured by real-time PCR, and the load of HBV DNA in serum and PBMCs was assessed by semi-quantitative analysis of gel imaging. RESULTS: The levels of IP-10 in serum and IP-10 mRNA in PBMCs of patients with cirrhosis were 299.9 +/- 77.2 pg/ml and 0.7500 +/- 0.1495, respectively. They were higher than those of controls (P<0.05) and also increased in the HBV DNA(+) groups (P<0.05, P<0.01) to 343.0 +/- 80.3 pg/ml and 0.8465 +/- 0.1528, respectively. The levels of IP-10 in serum and IP-10 mRNA in PBMCs were clearly correlated with the load of HBV DNA (P<0.01). CONCLUSIONS: The levels of IP-10 and IP-10 mRNA in the peripheral blood of patients with cirrhosis increase are closely correlated with the load of HBV DNA in serum, and play a key role in the progression of post-hepatitic cirrhosis. (Hepatobiliary Pancreat Dis Int 2010; 9: 280-286)

关键词： hepatic cirrhosis interferon gamma-inducible protein 10 mRNA HBV DNA peripheral blood mononuclear cells

Expression of CXC chemokine IP-10 in patients with chronic hepatitis B

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Hepatobiliary & Pancreatic Diseases International 2008年第1期7卷 45-50页

作者： Wang, jian Zhao, Jin-Hong Wang, ping-ping xiang, Gui-Ju Anhui Univ Sci & Technol Coll Med Dept Aetiol & Immunol Huainan 232001 Peoples R China

BACKGROUND: Chemokines have strong chemoattractant effects and are involved in a variety of immune and inflammatory reactions, such as attracting activated T lymphocytes, neutrophils, monocytes and natural killer cells via the pathway of G protein-coupled receptors to sites of inflammatory injury and contribute to wound repair. This investigation was designed to assess the levels of chemokine interferon-gamma inducible protein-10 (IP-10) and IP-10 mRNA, and the relationship between IP-10 mRNA and HBV-DNA and alanine aminotransferase (ALT) in patients with chronic hepatitis B. METHODS: The levels of IP-10 mRNA in peripheral blood mononuclear cells (PBMCs) were kinetically detected by real-time polymerase chain reaction (PCR). The rate of chemokine/GAPDH was regarded as the extreme level of chemokine. The level of IP-10 in serum was measured by enzyme linked immunosorbent assay (ELISA), and the expression of IP-10 in hepatic biopsy tissue was detected by streptavidin-peroxidase (SP) immunohistochemistry. RESULTS: The level of IP-10 mRNA in the PBMCs of patients was 0.7387 +/- 0.0768 (lg cDNA/lg GAPDH); it was significantly higher in patients with chronic hepatitis B than that in normal controls (P<0.001). The level of IP-10 in the serum of patients was 660.9 +/- 75.5 pg/ml. There was a significant difference between patients with chronic hepatitis B and normal controls (P<0.05). In patients with chronic hepatitis B, the level of IP-10 mRNA in PBMCs was correlated with the IP-10 plasma level (r=0.7312, P<0.001), and the IP-10 plasma level was fairly correlated with the levels of ALT and HBV-DNA plasma (r=0.7235, P<0.001; r=0.7371, P<0.001). IP-10 was found by immunohistochemical analysis to be selectively upregulated on sinusoidal endothelium. CONCLUSIONS: The expression of IP-10 mRNA in PBMCs, IP-10 plasma concentration and the expression of IP-10 in sinusoidal endothelium are all high in patients with chronic hepatitis B. Chemokine IP-10 may play an important role in

关键词： hepatitis B chemokine interferon-gamma inducible protein-10 sinusoidal endothelium

Morphodynamic Characteristics and Medium-Term Simulation of the North-South Passage Under the Impact of the Yangtze Estuary Deepwater Navigation Channel Project

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China Ocean Engineering 2020年第2期34卷 198-209页

作者： JIAO jian DOU Xi-ping GAO xiang-yu DING Lei YANG Xiao-yu Key Laboratory of Port Waterway and Sedimentation Engineering of MOTNanjing Hydraulic Research InstituteNanjing 210029China College of Water Conservancy and Hydropower Engineering Hohai UniversityNanjing 210098China

The morphological evolution characteristics of the North-South Passage area since the construction of the Yangtze Estuary Deepwater Navigation Channel Project(DNCP)are analyzed on the basis of the measured data.A twodimensional morphodynamics numerical model of the Yangtze Estuary is established to verify the morphological evolution of the North-South Passage under the influence of the DNCP and to predict the future evolution in the next 40 *** analysis shows that the North Passage has experienced rapid adjustment stages and adaptive stages after the construction of the *** erosion occurred along the main channel,and slow siltation could be observed in the area between the *** South Passage showed a state of upper section erosion and down section *** present,the whole South Passage is in a slight erosion *** to the numerical model,the eroding and silting speed of the North Passage will slow down in the *** present state that erosion occurs in the main channel and siltation occurs between the groins will *** South Passage will still maintain upper section erosion and down section deposition in the *** to the main channel erosion of the North Passage and siltation of the South Passage,the sediment division ratio of the North Passage will increase in the future but still be smaller than 50%.After morphological evolution of 40 years,the direction of residual sediment transport caused by M2 and M4 tidal components in the North Passage has not changed,but the transport rate will *** is considered that the morphological evolution of the North-South Passage could reach a relatively stable state after 40 years.

关键词： Yangtze Estuary numerical model morphology tidal current sediment transport deepwater navigation channel project(DNCP)

Entanglement Dynamics of Two Qubits Coupled to a Noise Environment

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Communications in Theoretical Physics 2009年第4期51卷 637-640页

作者： LIU Jin xiang Shao-Hua CUI Hui-ping LI jian Department of Physics School of Science Beijing Institute of Technology Beijing 100081 China Department of Physics and electronic Information Science Huaihua University Huaihua 418008 China

We study the time evolution of two two-state systems （two qubits） initially in the pure entangled states or the maximally entangled mixed states interacting with the individual environmental noise. It is shown that due to environment noise, all quantum entangled states are very fragile and become a classical mixed state in a short-time limit. But the environment can affect entanglement in very different ways. The type of decoherence process for certain entangled states belongs to amplitude damping, while the others belong to dephasing decoherenee.

关键词： decoherence entanglement pure state mixed state

Exothermic process of cast-in-place pile foundation and its thermal agitation of the frozen ground under a long dry bridge on the Qinghai-Tibet Railway

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2010年第2期11卷 88-96页

作者： Ya-ping WU jian GUO Chun-xiang GUO Wei MA Xiao-jun WANG School of Civil and Arehiteeture Engineering Ningbo Institute of Technology Zhejiang University Ningbo 315100 China School of Civil Engineering Lanzhou Jiaotong University Lanzhou 730070 China State Key Laboratory of Frozen Soil Engineering Cold and Arid Regions Environment and Engineering Research Institute Chinese Aeademy of Sciences Lanzhou 730000 China

A number of dry bridges have been built to substitute for the roadbed on the Qinghai-Tibet Railway,*** aim of this study was to investigate the exothermic process of cast-in-place (CIP) pile foundation of a dry bridge and its harm to the stability of nearby frozen *** present 3D heat conduction functions of a concrete pile and of frozen ground with related *** analysis is based on the theory of heat conduction and the exponent law describing the adiabatic temperature rise caused by hydration *** under continuous and initial conditions were combined to establish a finite element model of a CIP pile-frozen ground system for a dry bridge under actual field conditions in cold *** results indicated that the process could effectively simulate the exothermic process of CIP pile *** disturbance to frozen ground under a long dry bridge caused by the casting temperature and hydration heat of CIP piles was substantial and *** simulated thermal analysis results agreed with field measurements and some significant rules relating to the problem were deduced and conclusions reached.

关键词： Exothermic process of hydration heat Cast-in-place (CIP) pile foundation Dry bridge Thermal agitation Frozen ground Qinghai-Tibet Railway

Development and detection application of monoclonal antibodies against Zucchini yellow mosaic virus

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Journal of Integrative Agriculture 2017年第1期16卷 115-124页

作者： CHEN Zhe ZHANG Ming-hao ZHOU Xue-ping WU jian-xiang State Key Laboratory of Rice Biology Institute of BiotechnologyZhejiang University State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural Sciences

Aphid-borne Zucchini yellow mosaic virus （ZYMV） is one of the most economically important viruses of cucurbitaceous plants. To survey and control this virus, it is necessary to develop an efficient detection technique. Using purified ZYMV virion and the conventional hybridoma technology, three hybridoma cell lines （16A11, 5A7 and 3B8） secreting monoclonal antibodies （MAbs） against ZYMV Zhejiang isolate were obtained. The working titers of the ascitic fluids secreted by the three hybridoma cell lines were up to 10^-7 by indirect enzyme-linked immunosorbent assay （ELISA）. All MAbs were isotyped as IgG1, kappa light chain. Western blot analysis indicated that the MAb 3B8 could specifically react with the coat protein of ZYMV while MAbs 5A7 and 16A11 reacted strongly with a protein of approximately 51 kDa from the ZYMV-infected leaf tissues. According to this molecular weight, we consider this reactive protein As likely to be the HC-Pro protein. Using these three MAbs, we have now developed five detection assays, i.e., antigen-coated-plate ELISA （ACP-ELISA）, dot-ELISA, tissue blot-ELISA, double-antibody sandwich ELISA （DAS-ELISA）, and immunocapture-RT-PCR （IC-RT-PCR）, for the sensitive, specific, and easy detection of ZYMV. The sensitivity test revealed that ZYMV could be readily detected respectively by ACP-ELISA, dot-ELISA, DAS-ELISA and IC-RT-PCR in 1：163840, 1：2560, 1：327680 and 1：1 310720 （w/v, g mL-1） diluted crude extracts from the ZYMV-infected plants. We demonstrated in this study that the dot-ELISA could also be used to detect ZYMV in individual viruliferous aphids. A total of 275 cucurbitaceous plant samples collected from the Zhejiang, jiangsu, Shandong and Hainan provinces, China, were screened for the presence of ZYMV with the described assays. Our results showed that 163 of the 275 samples （59%） were infected with ZYMV. This finding indicates that ZYMV As now widely present in cucurbitaceous crops in China. RT-PCR followed by DNA sequencing and sequence a

关键词： Zucchini yellow mosaic virus monoclonal antibody ACP-ELISA dot-ELISA tissue blot-ELISA DAS-ELISA IC-RT-PCR

EFFECT OF SWEEP AND EJECTION EVENTS ON PARTICLE DISPERSION IN WALL BOUNDED TURBULENT FLOWS

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Journal of Hydrodynamics 2012年第5期24卷 794-799页

作者： LUO jian-ping LU Zhi-ming QIU xiang LI Dong-mei LIU Yu-lu School of Mechanical Engineering Shanghai Institute of Technology Shanghai 200235 China Shanghai Key Laboratory of Mechanics in Energy Engineering and Shanghai Institute of Applied Mathematicsand Mechanics Shanghai University Shanghai 200072 China School of Science Shanghai Institute of Technology Shanghai 200235 China Shanghai Institute of Technology Shanghai 200235 China

This paper studies the sweep and ejection events in a channel flow with Reτ = 80 by using Direct Numerical Simulation （DNS）. The effects of ejection and sweep events on the transport of fluid particles are analyzed separately through a quadrant technique. By analyzing trajectories of the particles released at different wall-normal locations, it is found that the particles from the ejection events mainly move upward while the particles from the sweep events move downward of the flow during short and intermediate diffusion time durations. Numerical results show that the effects of the ejection and sweep events on the mean displacement and the mean square dispersion remain for a long time, one-order of magnitude larger than the streamwise Lagrangian integral ~cales.

关键词： sweep ejection dispersion quadrant analysis turbulent channel flow

A Multistage Optical Stark Decelerator for use with a Pulsed Molecular Beam with an Electrostatic Storage Ring

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Chinese Physics Letters 2012年第5期29卷 97-99页

作者： XIA Yong YIN Ya-Ling JI xiang YIN jian-ping State Key Laboratory of Precision Spectroscopy Department of PhysicsEast China Normal UniversityShanghai 200062

We propose a promising scheme to repeatedly decelerate a pulsed molecular beam using a red-detuned quasi-cw semi-Gaussian laser beam (SGB) and an electrostatic storage *** the Monte-Carlo simulation method,we demonstrate that this promising optical Stark decelerator can be used to efficiently slow a pulsed ND3 molecular beam extracted from a Stark decelerator or a cryogenic reservoir by using a single *** deceleration effect of this scheme on the intensity of the SGB is discussed in detail.

关键词： Stark beam scheme

A novel and efficient route for the preparation of atorvastatin

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Chinese Chemical Letters 2011年第10期22卷 1159-1162页

作者： jian Gao Yang Hui Guo Ya ping Wang xiang Jing Wang Wen Sheng xiang Department of Biochemical Engineering Northeast Agricultural University Harbin 150030 China Zhejiang Hisun Pharmaceutical Co. Ltd. Taizhou 318000 China

A novel and efficient synthetic method of atorvastatin was *** key step of the synthesis was the construction of the olefin linkage between the chiral side chain and skeleton via a Horner-Wadsworth-Emmons reaction,resulting in the advanced intermediate of atorvastatin under hydrogenation of the olefin over Pd/*** novel method is more useful for the practical synthesis of atorvastatin than its document reported methods.

关键词： Atorvastatin Horner-Wadsworth-Emmons reaction Synthesis Intermediate