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Hepatitis B Virus X Protein Upregulates Intracellular Calcium Signaling by Binding C-terminal of Orai1 Protein

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Journal of huazhong University of Science and Technology(Medical Sciences) 2018年第1期38卷 26-34页

作者： Jing-hong YAO Zi-jian LIU jian-hua yi Jun WANG Ya-nan LIU Department of Infectious Diseases Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Anatomy School of Basic Medical SciencesTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Gastroenterology the Central Hospital of WuhanTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430014China

Hepatitis B virus X(HBx)protein plays a pivotal role in the development of hepatitis B virus(HBV)-associated hepatocellular *** regulation of cytosolic calcium is essential for HBV replication and is mediated by HBx protein,the mechanism of HBx protein regulating intracellular calcium level remains poorly *** present study examined whether HBx protein elevated the intracellular calcium through interacting with storeoperated calcium entry(SOCE)components,Orai1 and stromal interaction molecule 1,and then identified the targets of HBx protein,with an attempt to understand the mechanism of HBx protein upsetting intracellular calcium *** employing co-immunoprecipitation and GST-pull-down assay,we found that Orai1 protein interacted with HBx protein,and the C-terminus of Orai1 was implicated in the *** microscopy also revealed that HBx protein could co-localize with full-length Orai1 protein in HEK293 ***,live cell calcium imaging exhibited that HBx protein elevated intracellular calcium,possibly by binding to SOCE *** results suggest that HBx protein binds to STIM1-Orai1 complexes to positively regulate the activity of plasma membrane store-operated calcium channels.

关键词： HBx protein store-operated calcium entry Orai 1 stromal interaction molecule 1 intracellular calcium

Synthesis,crystal structure and thermal behavior of 4-amino-3,5-dinitropyrazole copper salt

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Chinese Chemical Letters 2014年第6期25卷 902-906页

作者： ying-Lei Wang Feng-Qi Zhao Yue-Ping Ji jian-hua yi Ting An Wei-Xiao Liu Science and Technology on Combustion and Explosion Laboratory Xi'an Modern Chemistry Research Institute

A novel energetic combustion catalyst, 4-amino-3,S-dinitropyrazole copper salt （[Cu（adnp）2（H2O）2]）, was synthesized in a yield of 93.6% for the first time. The single crystal of [Cu（adnp）2（H2O）2] was determined by single crystal X-ray diffraction. It crystallizes in a triclinic system, space group p^-1 with crystal parameters a = 5.541（3） A, b = 7.926（4） A, c = 10.231（5） A,β = 101.372（8）°, V = 398.3（3） A3, Z = 1, μ = 1.467 mm^-1, F（0 0 0） = 243, and Dc = 2.000 g cm^-3. The thermal behavior and non-isothermal decomposition reaction kinetics of [Cu（adnp）2（H2O）2] were studied by means of different heating rate differential scanning calorimetry （DSC）. The kinetic equation of major exothermic decomposition reaction for [Cu（adnp）2（H2O）2] was obtained. The entropy of activation （△S≠）, enthalpy of activation （△H≠）, free energy of activation （△G≠）, the self-accelerating decomposition temperature （TSADT） and the critical temperature of thermal explosion （Tb） are 59.42 j mol^-1 K^-1, 169.5 kJ mol^-1, 1141.26 kJ mol ^-1 457.3 K and 468.1 K, respectively.

关键词： Physical chemistry 4-Amino-3,5-dinitropyrazole copper salt Energetic combustion catalyst Crystal structure Thermal behavior

Role of epithelial-to-mesenchymal transition in the pulmonary fi brosis induced by paraquat in rats

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World Journal of Emergency Medicine 2021年第3期12卷 214-220页

作者： jian-hua yi Zhao-cai Zhang Mei-bian Zhang Xin He Hao-ran Lin Hai-wen huang Hai-bin Dai Yu-wen huang Emergency Department the Second Affi liated Hospital of Zhejiang University School of MedicineHangzhou 310009China Scientifi c Research Department the Second Affi liated Hospital of Zhejiang University School of MedicineHangzhou 310009China Zhejiang Provincial Center for Disease Control and Prevention Hangzhou 301051China Shangyu People’s Hospital Shaoxing 312300China Pharmacy Department the Second Affi liated Hospital of Zhejiang University School of MedicineHangzhou 310009China

BACKGROUND:This study aims to explore the characteristics of the epithelial-to-mesenchymal transition(EMT)process and its underlying molecular mechanisms in the period of paraquat(PQ)-induced pulmonary fi brosis(PF).METHODS:Picrosirius red staining and collagen volume fraction were utilized to evaluate the pathological changes of PQ-induced PF in ***,Western blot,and real-time reverse transcriptase-polymerase chain reaction(RT-PCR)were used to measure the protein and gene expression of EMT markers,EMT-associated transcription factors,and regulators of EMT-related pathways,***:The collagen deposition in the alveolar septum and increased PF markers were characteristics of pathological changes in PQ-induced PF,reached a peak on day 14 after PQ poisoning,and then decreased on day *** protein and gene expression of the fibrosis marker,EMT markers,transcription factors,and regulators of EMT-related signaling pathways signifi cantly increased at diff erent time points after PQ poisoning compared with corresponding controls(P0.05).CONCLUSIONS:EMT process plays a role in the PQ-induced PF,in which most PF and EMT markers have a peak phenomenon,and its underlying molecular mechanisms might be determined by further studies.

关键词： Pulmonary fibrosis Paraquat Epithelial-to-mesenchymal transition Rats

Pedicle screw fixation for cervical spine instability： clinical efficacy and safety analysis

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Chinese Medical Journal 2009年第17期122卷 1985-1989页

作者： LIU Yong HU jian-hua YU Ke-yi Department of O)thopaedics Peking Union Medical CollegeHospital Peking Union Medical College & Chinese Academy ofMedical Sciences Beijing 100730 China

Background Reports of applying cervical pedicle screw （CPS） system in the posterior cervical spine surgeries are limited because of its inherent risk of neurovascular injury. The clinical results of cervical spine instability treated with CPS system were retrospectively analyzed, and the clinical efficacy and safety of this management were evaluated. Methods Twenty-five patients with cervical spine instability undergoing posterior C3-C7 single/double door laminoplasty and free-hand CPS fixation as well as graft fusion were investigated; of whom, 3 were due to trauma, and 22 degenerative cervical pathogenesis. One hundred and fifty in total, CPSs were implanted in 5 cervical segments for 1 patient, 4 for 2, 3 for 18, and 2 for 4. Japanese Orthopaedic Association （JOA） score and its improvement rate, neck disability index （NDI）, segmental stability, pedicle cortex perforation rate and other complication-associated parameters were assessed. Results The average follow-up was 16.6 （6-30） months. Compared with pre-operative values, JOA score improved by 4.10±0.84 points on average （P 〈0.05） at 6 months post operation, with a mean improvement rate of 61%. While the pre-operative and 6-month post-operative NDI were 32.96_-＇_-~.13 and 16.84_＋4.40 （P 〈0.05）, respectively. At 6-month post-operation and the final follow-up, fused segments were stable. Pedicle cortex perforation rate was 8.0%, with no neurovascular complications observed. Conclusions Anatomizing the pre-operative radiographic data facilitates the precise operative design prior to surgery; and CPS system is capable of offering safe and satisfying outcomes in the management of cervical spine instability.

关键词： cervical pedicle screw fixation instability

Management of traumatic hemothorax by closed thoracic drainage using a central venous catheter

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第1期13卷 43-48页

作者： jian-hua yi1,hua-bo LIU2,Mao ZHANG1,Jun-song WU1,jian-xin YANG1,Jin-ming CHEN1,Shan-xiang XU1,jian-an WANG1(1Department of Emergency,the Second Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310009,China)(2Department of Emergency,Zhoushan Hospital of Zhejiang Province,Zhoushan 316004,China) Department of Emergency the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China.

Objective:To evaluate the efficacy and safety of the treatment of traumatic hemothorax by closed pleural drainage using a central venous catheter(CVC),compared with using a conventional chest ***:A prospective controlled study with the Ethics Committee approval was undertaken.A total of 407 patients with traumatic hemothorax were involved and they were randomly assigned to undergo closed pleural drainage with CVCs(n=214) or conventional chest tubes(n=193).The Seldinger technique was used for drainage by CVC,and the conventional technique for drainage by chest *** the residual volume of the hemothorax was less than 200 ml after the daily volume of drainage decreased to below 100 ml for two consecutive days,the treatment was considered *** correlative data of efficacy and safety between the two groups were analyzed using t or chi-squared tests with SPSS 13.0.A P value of less than 0.05 was taken as indicating statistical ***:Compared with the chest tube group,the operation time,fraction of analgesic treatment,time of surgical wound healing,and infection rate of surgical wounds were significantly decreased(P0.05),or in the mean catheter/tube indwelling time and mean medical costs of patients treated successfully(P>0.05).Conclusions:Management of medium or large traumatic hemothoraxes by closed thoracic drainage using CVC is minimally invasive and as effective as using a conventional large-bore chest *** complications can be prevented and it has the potential to replace the large-bore chest tube.

关键词： Central venous catheter Drainage Trauma Hemothorax

Clinical application of sternocleidomastoid myocutaneous composite resection of oral carcinoma modified flap after

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中国口腔颌面外科杂志 2008年第B5期6卷 91-92页

作者： Hui Ming Wang jian hua Liu yi Lin Zhi Yong Li Hui Yong Zhu Dept. of Oral and Maxillofaxial Surgery Ist Affiliated HospitalMedical College of Zhejiang University Hangzhou 310003China

Thermal Behavior and Decomposition Mechanism of Guanidinium Azotetrazolate（GUZT）

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Thermal Behavior and Decomposition Mechanism of Guanidinium ...

中国化学会第五届全国热分析动力学与热动力学学术会议

作者： Ting An Feng-Qi Zhao Qiong Wang Qing Pan Yang Luo jian-hua yi Science and Technology on Combustion and Explosion Laboratory Xi’an Modern Chemistry Research Institute

Guanidinium azotetrazolate（GUZT） is a kind of non-metallic azotetrazole *** of its merits such as:high enthalpy of formation,low combustion temperature,high molar and volumetric gas production,few combustion residues,*** is expected to be widely used in clean propulsion systems,new gas generators and low signature *** this study,we utilized condensed phase thermolysis/FTIR,TG-DTG and DSC techniques to study the thermal decomposition process of *** thermal behavior and decomposition mechanism of GUZT have been investigated by analysing its solid-phase thermal decomposition processes and tracing the emitted gases.

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“就近入学”政策的舆论分析

教育学报 2016年第6期12卷 17-22页

作者：蒋建华王乃弋张晴首都师范大学教育学院北京100048 北京师范大学教育学部北京100875

2014年,教育部重申义务教育就近入学并连续三年发文要求做好城市义务教育阶段就近入学,得到了社会的相应回应,出现了众多的舆论现象。运用百度对近几年涉及就近入学的舆论现象进行舆论搜索,根据舆论背后的学术关键词在中国知网进行学术... 详细信息

2014年,教育部重申义务教育就近入学并连续三年发文要求做好城市义务教育阶段就近入学,得到了社会的相应回应,出现了众多的舆论现象。运用百度对近几年涉及就近入学的舆论现象进行舆论搜索,根据舆论背后的学术关键词在中国知网进行学术搜索,配合一定的个案访谈,在此基础上进行舆论分析。发现,这些舆论现象可归结为舆论差异和舆论冲突。就舆论差异而言,既有赞成的声音,也有反对的意见,以赞成为主;就舆论冲突而言,突出体现在认知冲突和程序冲突,其实质在于价值冲突。对于舆论差异和舆论冲突,需要包容、需要消解,从而达成社会理解。

关键词：义务教育就近入学政策舆论分析舆论差异舆论冲突

Maintaining moderate levels of hypochlorous acid promotes neural stem cell proliferation and differentiation in the recovery phase of stroke

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Neural Regeneration Research 2025年第3期20卷 845-857页

作者： Lin-Yan huang yi-De Zhang Jie Chen Hai-Di Fan Wan Wang Bin Wang Ju-Yun Ma Peng-Peng Li Hai-Wei Pu Xin-yian Guo jian-Gang Shen Su-hua Qi School of Medical Technology Xuzhou Key Laboratory of Laboratory DiagnosticsXuzhou Medical UniversityXuzhouJiangsu ProvinceChina School of Pharmacy Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina Department of Laboratory Medicine Branch Hospital of Huai’an First People’s HospitalHuai’anJiangsu ProvinceChina Department of Laboratory Medicine the Affiliated Hospital of Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina School of Chinese Medicine The University of Hong KongHong Kong Special Administrative RegionChina

It has been shown clinically that continuous removal of ischemia/reperfusion-induced reactive oxygen species is not conducive to the recovery of late ***,previous studies have shown that excessive increases in hypochlorous acid after stroke can cause severe damage to brain *** previous studies have found that a small amount of hypochlorous acid still exists in the later stage of stroke,but its specific role and mechanism are currently *** simulate stroke in vivo,a middle cerebral artery occlusion rat model was established,with an oxygen-glucose deprivation/reoxygenation model established in vitro to mimic *** found that in the early stage(within 24 hours)of ischemic stroke,neutrophils produced a large amount of hypochlorous acid,while in the recovery phase(10 days after stroke),microglia were activated and produced a small amount of hypochlorous ***,in acute stroke in rats,hypochlorous acid production was prevented using a hypochlorous acid scavenger,taurine,or myeloperoxidase inhibitor,4-aminobenzoic acid *** results showed that high levels of hypochlorous acid(200μM)induced neuronal apoptosis after oxygen/glucose deprivation/***,in the recovery phase of the middle cerebral artery occlusion model,a moderate level of hypochlorous acid promoted the proliferation and differentiation of neural stem cells into neurons and *** suggests that hypochlorous acid plays different roles at different phases of cerebral ischemia/reperfusion *** levels of hypochlorous acid(5 and 100μM)promoted nuclear translocation ofβ-*** transfection of single-site mutation plasmids,we found that hypochlorous acid induced chlorination of theβ-catenin tyrosine 30 residue,which promoted nuclear ***,our study indicates that maintaining low levels of hypochlorous acid plays a key role in the recovery of neurological function.

关键词： cell differentiation cerebral ischemia/reperfusion injury chlorination hypochlorous acid microglia neural stem cell neurogenesis nuclear translocation stroke β-catenin