Role of epithelial-to-mesenchymal transition in the pulmonary fi brosis induced by paraquat in rats

作     者：Jian-hua Yi Zhao-cai Zhang Mei-bian Zhang Xin He Hao-ran Lin Hai-wen Huang Hai-bin Dai Yu-wen Huang Jian-hua Yi;Zhao-cai Zhang;Mei-bian Zhang;Xin He;Hao-ran Lin;Hai-wen Huang;Hai-bin Dai;Yu-wen Huang

作者机构：Emergency Departmentthe Second Affi liated Hospital of Zhejiang University School of MedicineHangzhou 310009China Scientifi c Research Departmentthe Second Affi liated Hospital of Zhejiang University School of MedicineHangzhou 310009China Zhejiang Provincial Center for Disease Control and PreventionHangzhou 301051China Shangyu People’s HospitalShaoxing 312300China Pharmacy Departmentthe Second Affi liated Hospital of Zhejiang University School of MedicineHangzhou 310009China 

出 版 物：《World Journal of Emergency Medicine》 (世界急诊医学杂志（英文）)

年 卷 期：2021年第12卷第3期

页      面：214-220页

核心收录：

学科分类：1002[医学-临床医学] 10[医学] 

基　　金：the National Natural Science Foundation of China(81472961) the Natural Science Foundation of Zhejiang Province(LY13H150001) the Zhejiang Provincial Program for the Cultivation of High-level Innovative Health Talents. 

主　　题：Pulmonary fibrosis Paraquat Epithelial-to-mesenchymal transition Rats 

摘      要：BACKGROUND:This study aims to explore the characteristics of the epithelial-to-mesenchymal transition(EMT)process and its underlying molecular mechanisms in the period of paraquat(PQ)-induced pulmonary fi brosis(PF).METHODS:Picrosirius red staining and collagen volume fraction were utilized to evaluate the pathological changes of PQ-induced PF in rats.Immunohistochemistry,Western blot,and real-time reverse transcriptase-polymerase chain reaction(RT-PCR)were used to measure the protein and gene expression of EMT markers,EMT-associated transcription factors,and regulators of EMT-related pathways,respectively.RESULTS:The collagen deposition in the alveolar septum and increased PF markers were characteristics of pathological changes in PQ-induced PF,reached a peak on day 14 after PQ poisoning,and then decreased on day 21.The protein and gene expression of the fibrosis marker,EMT markers,transcription factors,and regulators of EMT-related signaling pathways signifi cantly increased at diff erent time points after PQ poisoning compared with corresponding controls(P0.05).CONCLUSIONS:EMT process plays a role in the PQ-induced PF,in which most PF and EMT markers have a peak phenomenon,and its underlying molecular mechanisms might be determined by further studies.