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MicroRNA-219 alleviates glutamate-induced neurotoxicity in cultured hippocampal neurons by targeting calmodulin-dependent protein kinase Ⅱ gamma

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Neural Regeneration Research 2018年第7期13卷 1216-1224页

作者： Ting Wang Qun Cai Wen-Jie Yang Hai-Hua Fan jian-feng yi feng Xu Department of Emergency First Affiliated Hospital of Soochow University Suzhou Jiangsu Province China Department of Emergency Affiliated Hospital of Nantong University Nantong Jiangsu Province China Department of Pediatrics Affiliated Hospital of Nantong University Nantong Jiangsu Province China Medical College of Nantong University Nantong Jiangsu Province China

Septic encephalopathy is a frequent complication of sepsis,but there are few studies examining the role of micro RNAs（mi Rs） in its *** this study,a mi R-219 mimic was transfected into rat hippocampal neurons to model mi R-219 overexpression.A protective effect of mi R-219 was observed for glutamate-induced neurotoxicity of rat hippocampal neurons,and an underlying mechanism involving calmodulin-dependent protein kinase II γ（Ca MKIIγ） was *** R-219 and Ca MKIIγ m RNA expression induced by glutamate in hippocampal neurons was determined by quantitative real-time reverse transcription-polymerase chain reaction（q RT-PCR）.After neurons were transfected with mi R-219 mimic,effects on cell viability and apoptosis were measured by 3-（4,5-dimethylthiazolyl-2）-2,5-diphenyltetrazolium bromide（MTT） assay and flow *** addition,a luciferase reporter gene system was used to confirm Ca MKIIγ as a target gene of mi *** blot assay and rescue experiments were also utilized to detect Ca MKIIγ expression and further verify that mi R-219 in hippocampal neurons exerted its effect through regulation of Ca MKIIγ.MTT assay and q RT-PCR results revealed obvious decreases in cell viability and mi R-219 expression after glutamate stimulation,while Ca MKIIγ m RNA expression was ***,flow cytometry,and caspase-3 activity assays showed that mi R-219 overexpression could elevate glutamate-induced cell viability,and reduce cell apoptosis and caspase-3 ***,luciferase Ca MKIIγ-reporter activity was remarkably decreased by co-transfection with mi R-219 mimic,and the results of a rescue experiment showed that Ca MKIIγ overexpression could reverse the biological effects of mi ***,these findings verify that mi R-219 expression was decreased in glutamate-induced neurons,Ca MKIIγ was a target gene of mi R-219,and mi R-219 alleviated glutamate-induced neuronal excitotoxicity by negatively controlling Ca MKIIγ expression.

关键词： nerve regeneration brain injury septic encephalopathy miR-219 hippocampal neurons glutamate excitotoxicity apoptosis caspase-3 calmodulin-dependent protein kinase Ⅱ γ luciferase reporter gene system neuroprotection neural regeneration

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Mosaic Trisomy 21 and Trisomy 14 as Acquired Cytogenetic Abnormalities without GATA1 Mutation in A Pediatric Non-Down Syndrome Acute Megakaryoblastic Leukemia

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Chinese Journal of Cancer Research 2011年第3期23卷 239-241页

作者： yi Xiao Jia Wei Jin-huan Xu jian-feng Zhou yi-cheng Zhang Department of Hematology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

One case of acute megakaryoblastic leukemia （AMKL） with trisomy 21,trisomy 14 and unmutated GATA1 gene in a phenotypically normal girl was *** patient experienced transient myelodysplasia before the onset of *** bone marrow blasts manifested typical morphology of megakaryoblast both by the May-Giemsa staining and under the electronic *** cells were positive for CD13,CD33,CD117,CD56,CD38,CD41 and CD61 in flow cytometry *** study showed karyotype of 48,XX,＋14,＋21 in 40% *** mutations of GATA1 gene in Down syndrome or acquired trisomy 21 were not detected in this case.

关键词： Acute megakaryoblastic leukemia Myelodysplasia Cytogenetics GATA1

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基于黄斑距离优化的视网膜OCT图像层次分割方法

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通信学报 2017年第4期38卷 158-165页

作者：李建锋鲁易周吉首大学信息科学与工程学院湖南吉首416000 中南大学信息科学与工程学院湖南长沙410083

视网膜分层是视盘结构三维分析、青光眼三维特征提取的基础,为改善视网膜OCT图像的层次分割效果,提出了一个基于强度的二维视网膜黄斑OCT图像多层结构分割算法。分割方法通过预处理、滤波等操作,计算出视网膜OCT图像中每个A-scan的强度... 详细信息

视网膜分层是视盘结构三维分析、青光眼三维特征提取的基础,为改善视网膜OCT图像的层次分割效果,提出了一个基于强度的二维视网膜黄斑OCT图像多层结构分割算法。分割方法通过预处理、滤波等操作,计算出视网膜OCT图像中每个A-scan的强度和强度梯度值,能很好地分割出视网膜OCT图像中的RNFL上界、IS和OS分界线、RPE层下界等,并用最短距离计算的黄斑距离策略对黄斑部位分层结果进行再优化,从而实现视网膜OCT图像的层次分割。实验结果表明,所提算法优化效果好,时间复杂度低,运行速度较快。

关键词：视网膜黄斑光学相干断层扫描图像分割

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深度学习在故障诊断领域中的研究现状与挑战

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控制与决策 2017年第8期32卷 1345-1358页

作者：任浩屈剑锋柴毅唐秋叶欣重庆大学自动化学院重庆400044 电力传输设备与系统安全国家重点实验室重庆400044 航天发射场可靠性技术重点实验室海口570100

现代工业系统已呈现出向大型化、复杂化的方向发展,使得针对工业系统的故障诊断方法遇到一系列的技术难题.近年来,深度学习(deep learning)在特征提取与模式识别方面显示出独特的优势与潜力,将深度学习应用于解决复杂工业系统故障诊断... 详细信息

现代工业系统已呈现出向大型化、复杂化的方向发展,使得针对工业系统的故障诊断方法遇到一系列的技术难题.近年来,深度学习(deep learning)在特征提取与模式识别方面显示出独特的优势与潜力,将深度学习应用于解决复杂工业系统故障诊断的研究已初现端倪.为此,首先介绍几种典型的基于深度学习方法实现工业系统故障诊断方法;然后对基于深度学习实现故障诊断的主要思想和建模方法进行描述;最后总结和讨论了复杂工业系统故障的特点,并探讨了深度学习在实现复杂工业系统故障诊断方面所面临的挑战,展望了未来值得继续研究的方向.

关键词：深度学习复杂工业系统特征提取故障检测与识别

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Outcomes of Adults with Acute Lymphoblastic Leukemia After Autologous Hematopoietic Stem Cell Transplantation and the Significance of Pretransplantation Minimal Residual Disease： Analysis from a Single Center of China

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Chinese Medical Journal 2015年第15期128卷 2065-2071页

作者： Zhe Ding Ming-Zhe Han Shu-Lian Chen Qiao-Ling Ma Jia-Lin Wei Ai-Ming Pang Xiao-Yu Zhang Chen Liang jian-feng Yao yi-Geng Cao Si-Zhou feng Er-Lie jiang Hematopoietic Stem Cell Transplantation Center Institute of Hematology and Blood Diseases Hospital Peking Union Medical College and Chinese Academy of Medical Sciences Tianjin 300020 China

Background：The postremission therapics for adult patients generally contain consolidation chemotherapy,allogeneic hematopoietic stem cell transplantation and autologous hematopoietic stem cell transplantation （auto-HSCT）.Because of the various results from different centers,the optimal therapy for adult acute lymphoblastic leukemia （ALL） patients is still *** study aimed to better understand predictive factors and role of auto-HSCT in the postremission thcrapy for adult ALL ***：The outcomes of 135 adult patients with ALL,who received the first auto-HSCT in Hematopoietic Stem Cell Transplantation Center of Blood Diseases Hospital,Chinese Academy of Medical Sciences from January 1,1994 to February 28,2014,were retrospectively *** curves were estimated using the Kaplan-Meier method and simultaneous effects of multiple covariates were estimated with the Cox ***：Overall survival （OS） and disease-free survival （DFS） at 5 years for the whole cohort were 59.1 ± 4.5% and 59.0 ± 4.4%,*** cumulative nonrelapse mortality and relapse rate at 5 years were 4.5 ± 0.03% and 36.6 ± 0.19%.For both OS and DFS,acute T-cell lymphoblastic leukemia,high lactate dehydrogenase （LDH） at diagnosis,blast cell proportion ≥5% on the 15th day of induction therapy,and extramedullary infiltration before HSCT were the poor prognosis *** addition,age ≥35 years predicted poor *** T-ALL and high LDH were the independent undesirable factors associated with OS and DFS in Cox regression *** 44 patients who had results of pretransplantation minimal residual disease （MRD）,positive MRD （MRD ≥0.01%） indicated poor OS （P =0.044） and DFS （P =0.008）.Furthermore,for the standard risk group,the patients with negative MRD （MRD 〈0.01%） had better results （OS at 18 months was 90.0 ± 9.5%,while for the patients with positive MRD OS was 50.0 ± 35.4%,P =0.003;DFS at 18 months was 90.0 ± 9.5%,while for the positive MRD group DFS was 0%,P 〈 0.00

关键词： Acute Lymphoblastic Leukemia Adult Autologous Hematopoietic Stem Cell Transplantation Minimal Residual Disease Prognostic Factors

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Mn-SOD and CuZn-SOD polymorphisms and interactions with risk factors in gastric cancer

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World Journal of Gastroenterology 2010年第37期16卷 4738-4746页

作者： jian-feng yi Shi-Liang Kang Xiang-Ting Zeng Yu-Min Li Tao Liu Jun-Qiang Zhang Wen-Ting He Xun Li Wen-Ce Zhou The First College of Clinical Medicine of Lanzhou University Lanzhou 730000 Gansu Province China Key Laboratory of Digestive System Tumors Lanzhou University Second Hospital China Department of General Surgery The Fir st Hospital of Lanzhou Unive rsityLanzhou 730000Gansu ProvinceChina

AIM: To investigate the effects of superoxide dismutase (SOD) polymorphisms (rs4998557 , rs4880), Helicobacter pylori (H. pylori ) infection and environmental factors in gastric cancer (GC) and malignant potential of gastric precancerous lesions (GPL). METHODS: Copper-zinc superoxide dismutase (SOD1, CuZn-SOD)-G7958A (rs4998557 ) and manganese superoxide dismutase (SOD2, Mn-SOD)-Val16Ala (rs4880 ) polymorphisms were genotyped by SNaPshot multiplex polymerase chain reaction (PCR) in 145 patients with GPL (87 cases of gastric ulcer, 33 cases of gastric polyps and 25 cases of atrophic gastritis), 140 patients with GC and 147 healthy controls. H. pylori infection was detected by immunoblotting analysis. RESULTS: The SOD1-7958A allele was associated with a higher risk of gastric cancer [odds ratio (OR) = 3.01, 95% confidence intervals (95% CI): 1.83-4.95]. SOD216Ala/Val genotype was a risk factor for malignant potential of GPL (OR = 2.04, 95% CI: 1.19-3.49). SOD216Ala/genotype increased the risk of gastric cancer (OR = 2.85, 95% CI: 1.66-4.89). SOD1-7958A/genotype, SOD2-16Ala/genotype, alcohol drinking, positive family history and type Ⅰ H. pylori infection were associated with risk of gastric cancer, and there were additive interactions between the two genotypes and the other three risk factors. SOD2-16Ala/Val genotype and positive family history were associated with malignant potential of GPL and jointly contributed to a higher risk for malignant potential of GPL (OR = 7.71, 95% CI: 2.10-28.22). SOD1-7958A/genotype and SOD2-16Ala/genotype jointly contributed to a higher risk for gastric cancer (OR = 6.43, 95% CI: 3.20-12.91). CONCLUSION: SOD1-7958A/and SOD2-16Ala/-genotypes increase the risk of gastric cancer in Chinese Han population. SOD2-16Ala/-genotype is associated with malignant potential of GPL.

关键词： Copper-zinc superoxide dismutase Manganese superoxide dismutase Gastric cancer Gastric precancerous lesions Gene polymorphisms Interaction

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弱凝胶调驱体系性能评价研究

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日用化学工业 2019年第2期49卷 87-91页

作者：张继红冯建祎东北石油大学提高采收率国家重点实验室黑龙江大庆163318

针对具有储层非均质性强、渗透率低和孔隙结构复杂特点的储层,在注水开发后期,含水率急剧上升,普通的水驱和聚驱方法很难将油驱替出。复合离子型聚合物凝胶有很好的储层适应性,与注入污水配伍性好,成胶时间可控,吸附、滞留能力强,抗冲... 详细信息

针对具有储层非均质性强、渗透率低和孔隙结构复杂特点的储层,在注水开发后期,含水率急剧上升,普通的水驱和聚驱方法很难将油驱替出。复合离子型聚合物凝胶有很好的储层适应性,与注入污水配伍性好,成胶时间可控,吸附、滞留能力强,抗冲刷性好。其注入能力强,波及范围大,既可以封堵储层大孔道("调"的作用),又可以驱替储层剩余油("驱"的作用),能够满足调驱体系大剂量注入的要求。本文针对复合离子型凝胶调驱体系进行配方优选并进行性能评价实验,实验结果表明,该调驱体系具有较好的抗盐性、抗剪切性、热稳定性和黏弹性。

关键词：两性离子型聚合物调驱体系抗盐性抗剪切性热稳定性黏弹性

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On Self Recovery Mechanism and Additional Maneuverability of a Mini Mobile Robot

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Advances in Manufacturing 1999年第3期3卷 223-226页

作者： QIAN Jin wu SU jian liang GU jian feng HE Yong yi CHENG Wei ming ZHANG Ya nan (School of Mechanical and Electronic Engineering, Shanghai University) School of Mechanical and Electronic Engineering Shanghai University Shanghai China

Ordinary mobile robots have some kind of moving mechanisms attached to one rigid body. When working on rough terrain or in other hazard environments, there existed some possibilities that the robot will be turned up side down, thus causing losses to the robot's expedition. Multi bodied mobile robots provide a solution to that problem. Using active joints between bodies, the robot can recover from turnover situation by itself. In this paper, the authors discuss the joint arrangements and the additional maneuverability resulted from joints between body segments.

关键词： mobile robot self recovery multi bodied maneuverability

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Studies on the biosynthesis of arteannuin Ⅰ.The biosynthesis of arteannuinic acid in Artemisia annua L.

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Acta Chimica Sinica(English Edition) 1988年第4期 383-385页

作者： HUANG Jing-jian ZHOU feng-yi WU Lian-Fen ZHEN Gui-Hui Shanghai Institute of Organic Chemistry,Academia Sinica,Shanghai

Arteannuin(Qinghaosu)(1)is a very potent natural antimalarial isolated from theChinese medicinal herb,Artemisia annua L.(Qinghao).Its chemical structure elucidation andtotal synthesis have already been *** view of its unusual sesquiterpene structureof an endoperoxide ketal-acetal lactone and its specific biological activity,the authours havebeen studying the problem of the biosynthesis of 1 since *** the consideration of thegeneral isoprene principle in the biogenesis of the sesquiterpene and the finding of all the iso-

关键词： Studies on the biosynthesis of arteannuin The biosynthesis of arteannuinic acid in Artemisia annua L acid

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Prophylaxis of hepatitis B recurrence in post-liver transplantation patients with lamivudine-resistant YMDD mutant

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Chinese Medical Journal 2007年第16期120卷 1400-1403页

作者： ZHANG Qi CAI Chang-jie LU Ming-qiang LI Xi jianG Nan jianG Hua XU Chi LI Hua WANG Gen-shu yi Shu-hong ZHANG jian ZHANG Jun-feng yi Hui-min ZHANG ying-cai CHEN Gui-hua Liver Transplant Center Third Affiliated Hospital of Sun Yat-sen University Transplantation Research Institute of Sun Yat-sen University Guangzhou 510630 China

Background The most frequently used therapy for post-transplantation recurrence of hepatitis B virus （HBV） infection is lamivudine, but this drug is associated with a high resistance rate due to YMDD mutant. In preliminary reports, adefovir dipivoxil （ADV） has been shown to have activity against lamivudine-resistant strains of HBV. However, clinical experience in treatment of HBV infection after liver transplantation （LT） is still not entirely clear. This study was aimed to evaluate the prophylactic efficacy of ADV plus hepatitis B immunoglobulin （HBIG） in patients with YMDD mutant before LT. Methods From March 2004 to March 2006, 16 patients with chronic hepatitis B had lamivudine-resistant YMDD mutants detected prior to liver transplantation and received treatment with ADV plus additional intramuscular HBIG after LT as prophylaxis against graft reinfection. Tests for liver function, serum HBsAg, anti-HBs （HBIG）, HBeAg, anti-HBc, anti-HBe, HBV-DNA, and creatinine were assessed pre- or post-liver transplantation. Results The median follow-up of these patients post-liver transplantation was 19.4 months. Fifteen patients survived and one patient died of recurrence of hepatocellular carcinoma （HCC）. There was significant difference （10.98% vs. 2.26%, P〈0.05） in YMDD mutant rate between the patients with HBV-DNA over 106 copies/ml and those with HBV-DNA less than 106 copies/mi. Fifteen patients （93.8%） had undetectable HBV-DNA at 4 weeks and 1 （6.3%） at 6 months after LT. No hepatitis B recurrence was detected by persistent testing of HBsAg, HBeAg, and HBV-DNA and no increase of serum creatinine level associated with ADV was observed in any of the patients. Conclusion ADV combined with intramuscular HBIG can effectively prevent patients with pre-transplantation YMDD mutant from HBV recurrence after LT.

关键词： liver transplantation adefovir dipivoxil YMDD mutant HBV recurrence

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